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The map label for this gene is lptD [H]

Identifier: 189424300

GI number: 189424300

Start: 1281347

End: 1283464

Strand: Reverse

Name: lptD [H]

Synonym: Glov_1235

Alternate gene names: 189424300

Gene position: 1283464-1281347 (Counterclockwise)

Preceding gene: 189424301

Following gene: 189424299

Centisome position: 32.76

GC content: 58.17

Gene sequence:

>2118_bases
ATGCGTCTCCCTGTAAACGTAAGACGTGCATGTTGTAAGGATCCTATCTTGAAACTACGTACCTGCATTTTTCTACTATT
CCTGCTGATGCCGGCCCTGCTGCAGGCTGCCGAACAGACCGCCGCGCCCGGCATGGTTATCCGCGCCGATGTCATGAACC
ATGACGCTGCCAGCGATCAGATCAAGGCCAGCGGCAAGGTTGAGATGATCTGGCAGGACATGACCATGACCGCCGATGAG
GCCACCTTCAACCGCAGCAACCAGACCCTGGTGGCCAGAGGCAACGTCCATCTGGTCAAGGCCGGCGACACACTCTGGGG
CGATCAGCTGGTACTGGACACCGAGACCGGGCGGGCGGAGATGGAGAACGGCCATATCTTCATGGCCCAGGGCAACTTCA
GGGCTGACGGCCGACAGGTTGCCAAAACCGGTGATGATGATTATGCCCTGCGGGACGGCGGCCTGACCACCTGTGATGCA
GCCGTTCCCAGCTGGAAATTCGGGGCATCGGAACTGGATGTCACCCTGGAGGAGTTTGCCACCGGCAAACATGTCATCTT
CTATGTCAAAGACATCCCGGTCTTTTACTTCCCCTACATCATCCTGCCGGTCAAGCGCGAGCGTCAGTCCGGTTTCCTGT
TCCCCAAATTCGGCAACTCCTCAAAACGCGGTGCCGCCGTTGATATCCCCTTCTACTGGGCCATCTCCCCTTCGCAGGAG
GCGACCGTTAACCTGGATGTCCAGACCAAGCGGGGGGTCGGCCTGGGGCTTGACTACCGCTACAAGCTCAGCCGTAGCAG
TGAAGGCTCACTGGGCGGCTACCTGATTTACGACAACAACGAAAACAAGGAACGGGGTCAACTGGTGCAGTTCCACAAAG
AACTGTTCTCCGACAGTTTTTCCCTGATCAGCAGCCTTAACCTGACCAGCGACCGGAACTTCCTGACCGACTATGGCGAA
AAAAGCGGTGAGTACAACCGGCAGTACTATGATTCACGGATCGTACTGACCAAATTCTGGGATCACTGGCTGACCAGTGC
CCAGACCATCTATACCCAGGATTACTACACCGGCAGTAACACCACCACCCTGCAACGTGCGCCGGAGCTGTCACTGTATG
CGGTCCGGGAGCAACTTCCCTTCATCCCCAACCTCTATTTTGACCTGGACCTGCTGGCTACCAACTACTACCGCGAAAAA
GGGACCGATGGCCAGCGGGCCGTGCTGGAACCACGCCTGACCTCAACCAACAGCCTGTTTGACGGCCGGCTGAACCTCTC
CCTGTTCGGCAGCCTGCAGGTGCGGGGCTATAACACCAACCGGGTCGATCCCGGCCTCAAGGATGATCAACTGCTGGCCA
TACCAAAGGCCGGGGCCCAGATCAGCAGTTCCTTCAGCCGGATCTTTGACGTCTCATTTCTTGATCTGCAGCGCATGCGC
CATGAGCTGGTTCCCACCCTCTCCTATCTCTACGTCGGAGATAAGGACCAGAGCAGTGTGCCGCTCTTTGACCAGCTGGA
CCGTCTGAATGCCCAGCAGACCCTGGCATTCTCCCTCGCCAGCCACCTGGGCGGCCGGATAGCCAAAAAGGAAGGGGCAG
CAGAATACCGCAACCTGATGACCCTGCGTTTGTTGCAGAACTACAGTCTGAGCGGCAGCCGTACCGACCTGTTGACCCTG
ACCGATGATTCCCGGCGTTGGGGCGATCTGACCCTGGAGAGCGAGACCTGGGTACATCGCTCTGTCCGGCTGCTGGCCGA
TCTCCGCTACAGCCATTATGACCACCGTATCTCCAGCACCGCCAGCGGCGTCGAATACAATGATCTGCGCGGCAATGCGG
CCCGGATCAGCTACCGGATGGTTGACCGCCAACTTGACTACCTTGAAGGCGCTACAAGCATCGCCCTGACCAGGCCGCTC
TATGCGACCTACACCACCCGCTACTCATTTGATAAACATGACTTCCTTGAATCCTACTACGCCCTTGAGTTCCGCCATCA
GTGCTGGAGTGTGACCGCCGGCTACCGTGAACGCCCGAACGACAGGGCCTGGACTGTCAACTTCAACCTCACCGGTCTGT
TCGGCATCGGCAACAGCCCCACAGGAGGCGCACGATGA

Upstream 100 bases:

>100_bases
CCGGACCCGGTGACTTGATCGTAGCTGCCGGCTCACTCTTTCTGGTGGGTGAACTCAAGGCGCTGCTGGCCGGTGTTCCC
TGTGAAGCGGTGCGGGGGTA

Downstream 100 bases:

>100_bases
AACGTCCCCTGCCCAAACTGGTCTATGCCGATGCCCAGGGCCAGATCTACGACCACCCCTTTCTGACCATGGCCGGCATG
AGTGGCCCGGAAGCGGTTTT

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MRLPVNVRRACCKDPILKLRTCIFLLFLLMPALLQAAEQTAAPGMVIRADVMNHDAASDQIKASGKVEMIWQDMTMTADE
ATFNRSNQTLVARGNVHLVKAGDTLWGDQLVLDTETGRAEMENGHIFMAQGNFRADGRQVAKTGDDDYALRDGGLTTCDA
AVPSWKFGASELDVTLEEFATGKHVIFYVKDIPVFYFPYIILPVKRERQSGFLFPKFGNSSKRGAAVDIPFYWAISPSQE
ATVNLDVQTKRGVGLGLDYRYKLSRSSEGSLGGYLIYDNNENKERGQLVQFHKELFSDSFSLISSLNLTSDRNFLTDYGE
KSGEYNRQYYDSRIVLTKFWDHWLTSAQTIYTQDYYTGSNTTTLQRAPELSLYAVREQLPFIPNLYFDLDLLATNYYREK
GTDGQRAVLEPRLTSTNSLFDGRLNLSLFGSLQVRGYNTNRVDPGLKDDQLLAIPKAGAQISSSFSRIFDVSFLDLQRMR
HELVPTLSYLYVGDKDQSSVPLFDQLDRLNAQQTLAFSLASHLGGRIAKKEGAAEYRNLMTLRLLQNYSLSGSRTDLLTL
TDDSRRWGDLTLESETWVHRSVRLLADLRYSHYDHRISSTASGVEYNDLRGNAARISYRMVDRQLDYLEGATSIALTRPL
YATYTTRYSFDKHDFLESYYALEFRHQCWSVTAGYRERPNDRAWTVNFNLTGLFGIGNSPTGGAR

Sequences:

>Translated_705_residues
MRLPVNVRRACCKDPILKLRTCIFLLFLLMPALLQAAEQTAAPGMVIRADVMNHDAASDQIKASGKVEMIWQDMTMTADE
ATFNRSNQTLVARGNVHLVKAGDTLWGDQLVLDTETGRAEMENGHIFMAQGNFRADGRQVAKTGDDDYALRDGGLTTCDA
AVPSWKFGASELDVTLEEFATGKHVIFYVKDIPVFYFPYIILPVKRERQSGFLFPKFGNSSKRGAAVDIPFYWAISPSQE
ATVNLDVQTKRGVGLGLDYRYKLSRSSEGSLGGYLIYDNNENKERGQLVQFHKELFSDSFSLISSLNLTSDRNFLTDYGE
KSGEYNRQYYDSRIVLTKFWDHWLTSAQTIYTQDYYTGSNTTTLQRAPELSLYAVREQLPFIPNLYFDLDLLATNYYREK
GTDGQRAVLEPRLTSTNSLFDGRLNLSLFGSLQVRGYNTNRVDPGLKDDQLLAIPKAGAQISSSFSRIFDVSFLDLQRMR
HELVPTLSYLYVGDKDQSSVPLFDQLDRLNAQQTLAFSLASHLGGRIAKKEGAAEYRNLMTLRLLQNYSLSGSRTDLLTL
TDDSRRWGDLTLESETWVHRSVRLLADLRYSHYDHRISSTASGVEYNDLRGNAARISYRMVDRQLDYLEGATSIALTRPL
YATYTTRYSFDKHDFLESYYALEFRHQCWSVTAGYRERPNDRAWTVNFNLTGLFGIGNSPTGGAR
>Mature_705_residues
MRLPVNVRRACCKDPILKLRTCIFLLFLLMPALLQAAEQTAAPGMVIRADVMNHDAASDQIKASGKVEMIWQDMTMTADE
ATFNRSNQTLVARGNVHLVKAGDTLWGDQLVLDTETGRAEMENGHIFMAQGNFRADGRQVAKTGDDDYALRDGGLTTCDA
AVPSWKFGASELDVTLEEFATGKHVIFYVKDIPVFYFPYIILPVKRERQSGFLFPKFGNSSKRGAAVDIPFYWAISPSQE
ATVNLDVQTKRGVGLGLDYRYKLSRSSEGSLGGYLIYDNNENKERGQLVQFHKELFSDSFSLISSLNLTSDRNFLTDYGE
KSGEYNRQYYDSRIVLTKFWDHWLTSAQTIYTQDYYTGSNTTTLQRAPELSLYAVREQLPFIPNLYFDLDLLATNYYREK
GTDGQRAVLEPRLTSTNSLFDGRLNLSLFGSLQVRGYNTNRVDPGLKDDQLLAIPKAGAQISSSFSRIFDVSFLDLQRMR
HELVPTLSYLYVGDKDQSSVPLFDQLDRLNAQQTLAFSLASHLGGRIAKKEGAAEYRNLMTLRLLQNYSLSGSRTDLLTL
TDDSRRWGDLTLESETWVHRSVRLLADLRYSHYDHRISSTASGVEYNDLRGNAARISYRMVDRQLDYLEGATSIALTRPL
YATYTTRYSFDKHDFLESYYALEFRHQCWSVTAGYRERPNDRAWTVNFNLTGLFGIGNSPTGGAR

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=762, Percent_Identity=21.1286089238845, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 79880; Mature: 79880

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPVNVRRACCKDPILKLRTCIFLLFLLMPALLQAAEQTAAPGMVIRADVMNHDAASDQ
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
IKASGKVEMIWQDMTMTADEATFNRSNQTLVARGNVHLVKAGDTLWGDQLVLDTETGRAE
EECCCCEEEEEECCEECCCCHHCCCCCCEEEEECCEEEEECCCCCCCCEEEEECCCCCCE
MENGHIFMAQGNFRADGRQVAKTGDDDYALRDGGLTTCDAAVPSWKFGASELDVTLEEFA
ECCCEEEEEECCCCCCCHHHHCCCCCCEEEECCCCCEECCCCCCCCCCCHHHCEEHHHHC
TGKHVIFYVKDIPVFYFPYIILPVKRERQSGFLFPKFGNSSKRGAAVDIPFYWAISPSQE
CCCEEEEEEECCCEEEECEEEEEEEECCCCCEEEECCCCCCCCCCEEECEEEEEECCCCC
ATVNLDVQTKRGVGLGLDYRYKLSRSSEGSLGGYLIYDNNENKERGQLVQFHKELFSDSF
CEEEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
SLISSLNLTSDRNFLTDYGEKSGEYNRQYYDSRIVLTKFWDHWLTSAQTIYTQDYYTGSN
HHHHHCCCCCCCCHHHHCCCCCCCCCHHHHCCEEEEHHHHHHHHCCCEEEEEEEEEECCC
TTTLQRAPELSLYAVREQLPFIPNLYFDLDLLATNYYREKGTDGQRAVLEPRLTSTNSLF
CEEEECCCCCCEEHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEE
DGRLNLSLFGSLQVRGYNTNRVDPGLKDDQLLAIPKAGAQISSSFSRIFDVSFLDLQRMR
CCEEEEEEEEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHH
HELVPTLSYLYVGDKDQSSVPLFDQLDRLNAQQTLAFSLASHLGGRIAKKEGAAEYRNLM
HHHCCCEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
TLRLLQNYSLSGSRTDLLTLTDDSRRWGDLTLESETWVHRSVRLLADLRYSHYDHRISST
HHHHHHCCCCCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
ASGVEYNDLRGNAARISYRMVDRQLDYLEGATSIALTRPLYATYTTRYSFDKHDFLESYY
CCCCEECCCCCCHHEEEEHHHHHHHHHHCCCCEEEEECCEEEEEEEEECCCHHHHHHHHH
ALEFRHQCWSVTAGYRERPNDRAWTVNFNLTGLFGIGNSPTGGAR
HHHHHHHHHEECCCHHCCCCCCEEEEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MRLPVNVRRACCKDPILKLRTCIFLLFLLMPALLQAAEQTAAPGMVIRADVMNHDAASDQ
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
IKASGKVEMIWQDMTMTADEATFNRSNQTLVARGNVHLVKAGDTLWGDQLVLDTETGRAE
EECCCCEEEEEECCEECCCCHHCCCCCCEEEEECCEEEEECCCCCCCCEEEEECCCCCCE
MENGHIFMAQGNFRADGRQVAKTGDDDYALRDGGLTTCDAAVPSWKFGASELDVTLEEFA
ECCCEEEEEECCCCCCCHHHHCCCCCCEEEECCCCCEECCCCCCCCCCCHHHCEEHHHHC
TGKHVIFYVKDIPVFYFPYIILPVKRERQSGFLFPKFGNSSKRGAAVDIPFYWAISPSQE
CCCEEEEEEECCCEEEECEEEEEEEECCCCCEEEECCCCCCCCCCEEECEEEEEECCCCC
ATVNLDVQTKRGVGLGLDYRYKLSRSSEGSLGGYLIYDNNENKERGQLVQFHKELFSDSF
CEEEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
SLISSLNLTSDRNFLTDYGEKSGEYNRQYYDSRIVLTKFWDHWLTSAQTIYTQDYYTGSN
HHHHHCCCCCCCCHHHHCCCCCCCCCHHHHCCEEEEHHHHHHHHCCCEEEEEEEEEECCC
TTTLQRAPELSLYAVREQLPFIPNLYFDLDLLATNYYREKGTDGQRAVLEPRLTSTNSLF
CEEEECCCCCCEEHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEE
DGRLNLSLFGSLQVRGYNTNRVDPGLKDDQLLAIPKAGAQISSSFSRIFDVSFLDLQRMR
CCEEEEEEEEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHH
HELVPTLSYLYVGDKDQSSVPLFDQLDRLNAQQTLAFSLASHLGGRIAKKEGAAEYRNLM
HHHCCCEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
TLRLLQNYSLSGSRTDLLTLTDDSRRWGDLTLESETWVHRSVRLLADLRYSHYDHRISST
HHHHHHCCCCCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
ASGVEYNDLRGNAARISYRMVDRQLDYLEGATSIALTRPLYATYTTRYSFDKHDFLESYY
CCCCEECCCCCCHHEEEEHHHHHHHHHHCCCCEEEEECCEEEEEEEEECCCHHHHHHHHH
ALEFRHQCWSVTAGYRERPNDRAWTVNFNLTGLFGIGNSPTGGAR
HHHHHHHHHEECCCHHCCCCCCEEEEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]