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The map label for this gene is cysJ [H]

Identifier: 187735137

GI number: 187735137

Start: 741529

End: 742659

Strand: Direct

Name: cysJ [H]

Synonym: Amuc_0631

Alternate gene names: 187735137

Gene position: 741529-742659 (Clockwise)

Preceding gene: 187735136

Following gene: 187735139

Centisome position: 27.83

GC content: 60.83

Gene sequence:

>1131_bases
ATGACCACTGAACCATACGGCAAAAAAAATCCGTTTCCCGCTCCCGTCCTCTCCGTCAGGCATATCACCGGGGAAGGCAG
CCCCAAGGAAACCATCCACATTGAATACAGCCTGGACGGTTCCGGGATGAATTATATTGCCGGGGACGCCCTGGCCGTCA
TTCCCGCCAATGACCCCTCCCTGGCGGACGCCCTGATTGACAAGCTCGGCCTCAGCCCGGATTCCCAGGTTCCCACGCCC
GAAGGGGAAACCGCAAGCCTGAAAGACGCCCTGGTTCACTGCTATGACATTACCAATGTCAACAAAGCCCTGCTGACCAA
GTGGGCCGCCGCCTCCGGCAGCCAGGAGCTGGACACGCTTCTGGCCGGGGATAAGGACGCCCTCAGCGATTTCCTGTGGG
GCCGGGACGTGCTGGATCTGGCCACGGAATACCCCGCTTCCTTCGAATCCGCGGAGGCTTTTGTCGGCATCCTGAAAAAG
ATCATGCCGCGCCTTTATTCCATCGCCTCCAGCCCGAACGCCCATCCCGAACAGGTTCACCTGTGCGTGGGCGCGGTACG
CTATACGGCCCGTGACCGCAAGCGCGGCGGCGTTTGCTCCACCTATATGGCGGACCGCCTCCAGCCGGGCCATACGGCCA
GGGTATTCGTACATACCAACAAGAATTTCCGCCTTCCGGAAGACGGGGACACCCCCATCATCATGGTGGGCCCCGGCACC
GGCATCGCCCCTTTCCGGGCATTCTGGGAGGAACGCGCCGCTTCCGGCGCCAAGGGCGGCAACTGGCTGTTCTTCGGCAA
TCCGTACAAGGCCACCGACTTCTGTTATGAAGACGAGCTGAACCAGTTGACCGGCACCGGCAAACTGAAGTTGTCCGTGG
CCTGGTCCCGAGACCAGGAAAAGAAGGTGTATGTGCAGCACCTCATGGTCCAGGAAGGCGAAGAGCTCTGGAAATGGCTG
GAAGCCGGCGCCTGCTTCTACGTCTGCGGGGACGCCTCCCGCATGGCGAAGGATGTGGACAACGCCCTTCATGAAGTCAT
CCAGACCTGGGGCCATAAAACTCCGGAGGAAGCCGCGGCATATGTGGCGGACATGAAACAGCACCGCCGTTACCAGCGGG
ACGTGTACTAA

Upstream 100 bases:

>100_bases
AAGGGAGACAACGTCGTCCTGGTCACATTCGGCGCCGGGCTTACCTGGTCCGCCGCCGTTATCCGCTGGTAATATTCTTT
TTCACCCCCCCTCCATCATC

Downstream 100 bases:

>100_bases
TTCAGGATCTCCCCCTTGAACCTGTCACCGCGCCTTTCCATTTTTTCTGGAAAGGCGCTTTTTCTTTTTCCGGAGAAAAC
CCGCCACGCATCATCACTCC

Product: FAD-binding domain protein

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTTEPYGKKNPFPAPVLSVRHITGEGSPKETIHIEYSLDGSGMNYIAGDALAVIPANDPSLADALIDKLGLSPDSQVPTP
EGETASLKDALVHCYDITNVNKALLTKWAAASGSQELDTLLAGDKDALSDFLWGRDVLDLATEYPASFESAEAFVGILKK
IMPRLYSIASSPNAHPEQVHLCVGAVRYTARDRKRGGVCSTYMADRLQPGHTARVFVHTNKNFRLPEDGDTPIIMVGPGT
GIAPFRAFWEERAASGAKGGNWLFFGNPYKATDFCYEDELNQLTGTGKLKLSVAWSRDQEKKVYVQHLMVQEGEELWKWL
EAGACFYVCGDASRMAKDVDNALHEVIQTWGHKTPEEAAAYVADMKQHRRYQRDVY

Sequences:

>Translated_376_residues
MTTEPYGKKNPFPAPVLSVRHITGEGSPKETIHIEYSLDGSGMNYIAGDALAVIPANDPSLADALIDKLGLSPDSQVPTP
EGETASLKDALVHCYDITNVNKALLTKWAAASGSQELDTLLAGDKDALSDFLWGRDVLDLATEYPASFESAEAFVGILKK
IMPRLYSIASSPNAHPEQVHLCVGAVRYTARDRKRGGVCSTYMADRLQPGHTARVFVHTNKNFRLPEDGDTPIIMVGPGT
GIAPFRAFWEERAASGAKGGNWLFFGNPYKATDFCYEDELNQLTGTGKLKLSVAWSRDQEKKVYVQHLMVQEGEELWKWL
EAGACFYVCGDASRMAKDVDNALHEVIQTWGHKTPEEAAAYVADMKQHRRYQRDVY
>Mature_375_residues
TTEPYGKKNPFPAPVLSVRHITGEGSPKETIHIEYSLDGSGMNYIAGDALAVIPANDPSLADALIDKLGLSPDSQVPTPE
GETASLKDALVHCYDITNVNKALLTKWAAASGSQELDTLLAGDKDALSDFLWGRDVLDLATEYPASFESAEAFVGILKKI
MPRLYSIASSPNAHPEQVHLCVGAVRYTARDRKRGGVCSTYMADRLQPGHTARVFVHTNKNFRLPEDGDTPIIMVGPGTG
IAPFRAFWEERAASGAKGGNWLFFGNPYKATDFCYEDELNQLTGTGKLKLSVAWSRDQEKKVYVQHLMVQEGEELWKWLE
AGACFYVCGDASRMAKDVDNALHEVIQTWGHKTPEEAAAYVADMKQHRRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=400, Percent_Identity=34, Blast_Score=208, Evalue=8e-54,
Organism=Homo sapiens, GI127139033, Length=404, Percent_Identity=33.6633663366337, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI221316705, Length=409, Percent_Identity=33.2518337408313, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI221316709, Length=400, Percent_Identity=33.5, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI24041029, Length=402, Percent_Identity=32.3383084577114, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI221316707, Length=357, Percent_Identity=33.0532212885154, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI10835173, Length=409, Percent_Identity=31.7848410757946, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI40254422, Length=409, Percent_Identity=29.3398533007335, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI169790956, Length=426, Percent_Identity=28.169014084507, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI169790958, Length=426, Percent_Identity=28.169014084507, Blast_Score=138, Evalue=9e-33,
Organism=Escherichia coli, GI1789123, Length=375, Percent_Identity=45.0666666666667, Blast_Score=317, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17554134, Length=402, Percent_Identity=33.3333333333333, Blast_Score=215, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17566446, Length=369, Percent_Identity=28.9972899728997, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17531441, Length=413, Percent_Identity=23.9709443099274, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321832, Length=437, Percent_Identity=29.7482837528604, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321143, Length=393, Percent_Identity=28.2442748091603, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6325305, Length=397, Percent_Identity=27.455919395466, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24582192, Length=401, Percent_Identity=34.1645885286783, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137192, Length=401, Percent_Identity=34.1645885286783, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI78706872, Length=374, Percent_Identity=31.0160427807487, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24583543, Length=374, Percent_Identity=31.0160427807487, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI78706876, Length=374, Percent_Identity=31.0160427807487, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24660907, Length=250, Percent_Identity=32.8, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24660903, Length=250, Percent_Identity=32.8, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647438, Length=386, Percent_Identity=23.8341968911917, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 41445; Mature: 41314

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEPYGKKNPFPAPVLSVRHITGEGSPKETIHIEYSLDGSGMNYIAGDALAVIPANDPS
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCEECCCEEEEEECCCCH
LADALIDKLGLSPDSQVPTPEGETASLKDALVHCYDITNVNKALLTKWAAASGSQELDTL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHH
LAGDKDALSDFLWGRDVLDLATEYPASFESAEAFVGILKKIMPRLYSIASSPNAHPEQVH
HCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LCVGAVRYTARDRKRGGVCSTYMADRLQPGHTARVFVHTNKNFRLPEDGDTPIIMVGPGT
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECCCEECCCCCCCCEEEECCCC
GIAPFRAFWEERAASGAKGGNWLFFGNPYKATDFCYEDELNQLTGTGKLKLSVAWSRDQE
CCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCH
KKVYVQHLMVQEGEELWKWLEAGACFYVCGDASRMAKDVDNALHEVIQTWGHKTPEEAAA
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YVADMKQHRRYQRDVY
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTEPYGKKNPFPAPVLSVRHITGEGSPKETIHIEYSLDGSGMNYIAGDALAVIPANDPS
CCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCEECCCEEEEEECCCCH
LADALIDKLGLSPDSQVPTPEGETASLKDALVHCYDITNVNKALLTKWAAASGSQELDTL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHH
LAGDKDALSDFLWGRDVLDLATEYPASFESAEAFVGILKKIMPRLYSIASSPNAHPEQVH
HCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LCVGAVRYTARDRKRGGVCSTYMADRLQPGHTARVFVHTNKNFRLPEDGDTPIIMVGPGT
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECCCEECCCCCCCCEEEECCCC
GIAPFRAFWEERAASGAKGGNWLFFGNPYKATDFCYEDELNQLTGTGKLKLSVAWSRDQE
CCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCH
KKVYVQHLMVQEGEELWKWLEAGACFYVCGDASRMAKDVDNALHEVIQTWGHKTPEEAAA
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YVADMKQHRRYQRDVY
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]