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The map label for this gene is leuA [H]

Identifier: 161621797

GI number: 161621797

Start: 1022078

End: 1023781

Strand: Direct

Name: leuA [H]

Synonym: PPA0941

Alternate gene names: 161621797

Gene position: 1022078-1023781 (Clockwise)

Preceding gene: 50842423

Following gene: 50842428

Centisome position: 39.92

GC content: 59.1

Gene sequence:

>1704_bases
ATGCCCAGCGGGCGTTATAAACCTTTCGTTCCGCACACGTTGCCGGACCGTACCTGGCCAGACAAGACCTTGGACCATGC
TCCGCGGTGGCTGTCCACTGATTTGCGTGATGGCAACCAGGCGCTTGTTGACCCCATGACTCCGGCTCGCAAGATGCGGA
TGTTCGAACTTCTTGTTGGGATGGGGTACAAGGAGATTGAAATTGGCTTCCCCTCAGCTTCTCAAACGGATTTCGACTTC
GTTCGGCAGGTTATTGAGTCCGGCATTCTCCCTGACGACGTCACTATTTCGGTGCTGACCCAGGCCCGTGAGGATCTCAT
CGAGCGCACCATGCGGAGCTTAGTCGGCGCTCACCGTGCCACTGTCCACATGTATAATGCAGTTGCCGAACTCTTCCGAC
GCGTTGTCTTCAACATCGACGAAAAGCAGTGCATTGACCTGGCGCACCGTGGTACTGAGTGGGTCGTCAGGTACGCCGAC
AAGTACCTCGGCGACGTTGAGTTCGGCTACGAGTACTCTCCGGAGATGTTTAGCCAGACCCGCACGGACTTCGCTATCGA
CGTCTGTCACTCCGTGATGGACGTGTGGCAGCCGGGGCCAGGCCGTGAGATTATCCTTAATCTGCCGGCTACCGTCGAGA
TGAGTACTCCGAACACCTACGCCGACCAAATCGAGTACTTCTGCCGCAATATCCGTGATCGTGAGCACGTGTGCGTCTCT
TTGCACCCGCACAATGATCGTGGCACGGCGATCGCGGCCGCCGAGTTCGCGCAGATGGCGGGCGCCGATCGCGTCGAGGG
CTGTCTCTTTGGCCACGGCGAGCGCACCGGCAACGTTGATCTCGTCACCTTAGGTATGAATCTTGTCAGCCAGGGAGTTG
ACGCGGGTATCGACTTCTCTGACATGCCAAAAATCCGCCGCACCGTCGAGTACTGCACTGGACTACCGGTGCCGGCACGC
CAGCCCTACGCCGGCGACCTCGTCTTCACAGCCTTTTCGGGCTCGCACCAAGACGCTATCAAGAAAGGCCTGGAGGACCT
CGAACGCCAGGCCCAGGAGCAGGGCATCGGTATCCACGAGGTTGACTGGCAGGCCCCCTACCTGACCATCGACCCACACG
ACGTCGGACGCACCTACGAGGCTGTCATTCGCGTCAACAGCCAGTCCGGTAAGGGCGGCATGGCCTACCTCCTGAAGACC
GAGCACCAAGTCGACCTGCCGCGTCGTCTGCAGATCGAGTTCTCCCGTGCAGTGCAGAATTACACCGATGACCAAGCCCG
CGAGATCAGCCACGACGAGATCTGGGATCTGTTTAACCGCGAGTATCTGCAAATGCCTGGCATCGAGCTGGTGAGCCGCA
CGACGTCCACCGAAACCGGTACCTATCAGCTCACCGCCAACCTTCGGATTAATGGGGCGGACACTCAGATCCGTGGTGAG
GGTAACGGTGCGATGTCGGCTTTCATCGACGCACTGGTCTCCCTCGACATCGTTGTCCACGTGCTCGACTACTCCGAGCA
CGCCATGACAGCGGGTGGTACTGCGAGGGCCGCCGCGTATGTCGAGTGTGAAATTGGGGACGGCGAGACCTCACAAATTC
TATGGGGAGTCGGCATTGACCCTGATATCACCCAGGCTTCCCTGAAGGCGGTCGTCAACGCGGTAAACCGCTCCCGTCGG
CAAACCTTGGATAGGGAGTCATGA

Upstream 100 bases:

>100_bases
GGCGGCCTCCTTGCTGCGGGTGATCGGGCTGCCCTACACATCACACACGGGAGACACCATGACGCACATTCAGCCCCGTC
CCTGCCAGCTGACTAGCCCG

Downstream 100 bases:

>100_bases
CGCCGTGATCATGGGGTGGGGTTGACCTCATTGGGGACGGCCCCACCAAATCTCCGGTCCCGCTTGGCGTATTCCATCAC
TGCGGCCCAGAGGTCGCGGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MPSGRYKPFVPHTLPDRTWPDKTLDHAPRWLSTDLRDGNQALVDPMTPARKMRMFELLVGMGYKEIEIGFPSASQTDFDF
VRQVIESGILPDDVTISVLTQAREDLIERTMRSLVGAHRATVHMYNAVAELFRRVVFNIDEKQCIDLAHRGTEWVVRYAD
KYLGDVEFGYEYSPEMFSQTRTDFAIDVCHSVMDVWQPGPGREIILNLPATVEMSTPNTYADQIEYFCRNIRDREHVCVS
LHPHNDRGTAIAAAEFAQMAGADRVEGCLFGHGERTGNVDLVTLGMNLVSQGVDAGIDFSDMPKIRRTVEYCTGLPVPAR
QPYAGDLVFTAFSGSHQDAIKKGLEDLERQAQEQGIGIHEVDWQAPYLTIDPHDVGRTYEAVIRVNSQSGKGGMAYLLKT
EHQVDLPRRLQIEFSRAVQNYTDDQAREISHDEIWDLFNREYLQMPGIELVSRTTSTETGTYQLTANLRINGADTQIRGE
GNGAMSAFIDALVSLDIVVHVLDYSEHAMTAGGTARAAAYVECEIGDGETSQILWGVGIDPDITQASLKAVVNAVNRSRR
QTLDRES

Sequences:

>Translated_567_residues
MPSGRYKPFVPHTLPDRTWPDKTLDHAPRWLSTDLRDGNQALVDPMTPARKMRMFELLVGMGYKEIEIGFPSASQTDFDF
VRQVIESGILPDDVTISVLTQAREDLIERTMRSLVGAHRATVHMYNAVAELFRRVVFNIDEKQCIDLAHRGTEWVVRYAD
KYLGDVEFGYEYSPEMFSQTRTDFAIDVCHSVMDVWQPGPGREIILNLPATVEMSTPNTYADQIEYFCRNIRDREHVCVS
LHPHNDRGTAIAAAEFAQMAGADRVEGCLFGHGERTGNVDLVTLGMNLVSQGVDAGIDFSDMPKIRRTVEYCTGLPVPAR
QPYAGDLVFTAFSGSHQDAIKKGLEDLERQAQEQGIGIHEVDWQAPYLTIDPHDVGRTYEAVIRVNSQSGKGGMAYLLKT
EHQVDLPRRLQIEFSRAVQNYTDDQAREISHDEIWDLFNREYLQMPGIELVSRTTSTETGTYQLTANLRINGADTQIRGE
GNGAMSAFIDALVSLDIVVHVLDYSEHAMTAGGTARAAAYVECEIGDGETSQILWGVGIDPDITQASLKAVVNAVNRSRR
QTLDRES
>Mature_566_residues
PSGRYKPFVPHTLPDRTWPDKTLDHAPRWLSTDLRDGNQALVDPMTPARKMRMFELLVGMGYKEIEIGFPSASQTDFDFV
RQVIESGILPDDVTISVLTQAREDLIERTMRSLVGAHRATVHMYNAVAELFRRVVFNIDEKQCIDLAHRGTEWVVRYADK
YLGDVEFGYEYSPEMFSQTRTDFAIDVCHSVMDVWQPGPGREIILNLPATVEMSTPNTYADQIEYFCRNIRDREHVCVSL
HPHNDRGTAIAAAEFAQMAGADRVEGCLFGHGERTGNVDLVTLGMNLVSQGVDAGIDFSDMPKIRRTVEYCTGLPVPARQ
PYAGDLVFTAFSGSHQDAIKKGLEDLERQAQEQGIGIHEVDWQAPYLTIDPHDVGRTYEAVIRVNSQSGKGGMAYLLKTE
HQVDLPRRLQIEFSRAVQNYTDDQAREISHDEIWDLFNREYLQMPGIELVSRTTSTETGTYQLTANLRINGADTQIRGEG
NGAMSAFIDALVSLDIVVHVLDYSEHAMTAGGTARAAAYVECEIGDGETSQILWGVGIDPDITQASLKAVVNAVNRSRRQ
TLDRES

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=540, Percent_Identity=23.1481481481481, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324225, Length=567, Percent_Identity=47.9717813051146, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6324682, Length=569, Percent_Identity=47.2759226713532, Blast_Score=538, Evalue=1e-153,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63438; Mature: 63307

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00086 CYTOCHROME_P450 ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSGRYKPFVPHTLPDRTWPDKTLDHAPRWLSTDLRDGNQALVDPMTPARKMRMFELLVG
CCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHH
MGYKEIEIGFPSASQTDFDFVRQVIESGILPDDVTISVLTQAREDLIERTMRSLVGAHRA
CCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVHMYNAVAELFRRVVFNIDEKQCIDLAHRGTEWVVRYADKYLGDVEFGYEYSPEMFSQT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
RTDFAIDVCHSVMDVWQPGPGREIILNLPATVEMSTPNTYADQIEYFCRNIRDREHVCVS
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEE
LHPHNDRGTAIAAAEFAQMAGADRVEGCLFGHGERTGNVDLVTLGMNLVSQGVDAGIDFS
EECCCCCCCCHHHHHHHHHCCCCHHCCEEEECCCCCCCEEEEEECHHHHHCCCCCCCCHH
DMPKIRRTVEYCTGLPVPARQPYAGDLVFTAFSGSHQDAIKKGLEDLERQAQEQGIGIHE
CCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEE
VDWQAPYLTIDPHDVGRTYEAVIRVNSQSGKGGMAYLLKTEHQVDLPRRLQIEFSRAVQN
ECCCCCEEEECCHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHC
YTDDQAREISHDEIWDLFNREYLQMPGIELVSRTTSTETGTYQLTANLRINGADTQIRGE
CCCHHHHHCCHHHHHHHHCHHHHCCCCHHHHHCCCCCCCCEEEEEEEEEEECCCCEEEEC
GNGAMSAFIDALVSLDIVVHVLDYSEHAMTAGGTARAAAYVECEIGDGETSQILWGVGID
CCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEECCCCCCCEEEEECCCC
PDITQASLKAVVNAVNRSRRQTLDRES
CCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PSGRYKPFVPHTLPDRTWPDKTLDHAPRWLSTDLRDGNQALVDPMTPARKMRMFELLVG
CCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHH
MGYKEIEIGFPSASQTDFDFVRQVIESGILPDDVTISVLTQAREDLIERTMRSLVGAHRA
CCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVHMYNAVAELFRRVVFNIDEKQCIDLAHRGTEWVVRYADKYLGDVEFGYEYSPEMFSQT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
RTDFAIDVCHSVMDVWQPGPGREIILNLPATVEMSTPNTYADQIEYFCRNIRDREHVCVS
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEE
LHPHNDRGTAIAAAEFAQMAGADRVEGCLFGHGERTGNVDLVTLGMNLVSQGVDAGIDFS
EECCCCCCCCHHHHHHHHHCCCCHHCCEEEECCCCCCCEEEEEECHHHHHCCCCCCCCHH
DMPKIRRTVEYCTGLPVPARQPYAGDLVFTAFSGSHQDAIKKGLEDLERQAQEQGIGIHE
CCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEE
VDWQAPYLTIDPHDVGRTYEAVIRVNSQSGKGGMAYLLKTEHQVDLPRRLQIEFSRAVQN
ECCCCCEEEECCHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHC
YTDDQAREISHDEIWDLFNREYLQMPGIELVSRTTSTETGTYQLTANLRINGADTQIRGE
CCCHHHHHCCHHHHHHHHCHHHHCCCCHHHHHCCCCCCCCEEEEEEEEEEECCCCEEEEC
GNGAMSAFIDALVSLDIVVHVLDYSEHAMTAGGTARAAAYVECEIGDGETSQILWGVGID
CCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEECCCCCCCEEEEECCCC
PDITQASLKAVVNAVNRSRRQTLDRES
CCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA