Click here to switch to the map view.

The map label for this gene is estB [H]

Identifier: 158318206

GI number: 158318206

Start: 7866573

End: 7867244

Strand: Reverse

Name: estB [H]

Synonym: Franean1_6471

Alternate gene names: 158318206

Gene position: 7867244-7866573 (Counterclockwise)

Preceding gene: 158318207

Following gene: 158318205

Centisome position: 87.59

GC content: 65.62

Gene sequence:

>672_bases
ATGTCCAGAGCGGCCAGGATCCTTGGCGCTCTCGCGGTCGCCGCCGCAGCTTTGCTGGGCACGTCGACCGCCGCCTCCGC
GGCGACACATGAACCCGTCATCTTCGTCTACGGATTCAACGGATCGACATCCACCTGGACCGACATGGTCGCCGACTTCC
AGGCCAACGGCTGGTCGTCGTCCGACCTGGTCGTGTTCACCTACGACTCGCACCAGTCGAACGTGACGACCGCGAACCAG
CTGGCCGCCAGAATCTCCTCCGTGCGCAGCGCGACCGGCGCGGCCAAGGTGGACCTCGTCACCCACTCCATGGGCGCGCT
GTCCTCGCGCTATTACCTCAAGAACCTCGGCGGGACGTCCGTCGTCGACGACTTCGTGTCGCTGGGTGGCCCGAACCACG
GGACCAGCACCGCGACCCTGTGCGGATGGCTCTACACGGCCTGCTCGGAGATGAGCCCGGGATCGTCCTTCCTGAACACC
CTGAACTCCGGTGACGAGACTCCGGGCGCGGTCAACTACGGTACGTGGTGGTCGTCCTGCGACGACACCATCAACCCGGA
TTCCAGCGTCTCCCTCTCGGGTGCCACGAACACCGCCGCCGGTTGCCTCGGCCATTCCGAGCTGCACGACGACAACACCG
TGGCCAGTCAGGTCAGAGCCTTCATCGGTTGA

Upstream 100 bases:

>100_bases
CCGTGACGGCAACCGTTGGTGAACGTCCCACGGATTCAAAGGGAGCTCCCCGCGGGGATGCAAGGCTTGCTGCGCTGACC
CCGCAACGACAGGAGTTCCC

Downstream 100 bases:

>100_bases
GCTGGGCTGAGCCGGGCTCAGCCGCCGAGCGGGCGGCTAACGAGCGATCCTGGTCCGGTGTGTGCGAACGTCCCGGGAAC
GGGTACGCCGAGGCGCACCA

Product: lipase class 2

Products: NA

Alternate protein names: Lipase B; Triacylglycerol lipase [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSRAARILGALAVAAAALLGTSTAASAATHEPVIFVYGFNGSTSTWTDMVADFQANGWSSSDLVVFTYDSHQSNVTTANQ
LAARISSVRSATGAAKVDLVTHSMGALSSRYYLKNLGGTSVVDDFVSLGGPNHGTSTATLCGWLYTACSEMSPGSSFLNT
LNSGDETPGAVNYGTWWSSCDDTINPDSSVSLSGATNTAAGCLGHSELHDDNTVASQVRAFIG

Sequences:

>Translated_223_residues
MSRAARILGALAVAAAALLGTSTAASAATHEPVIFVYGFNGSTSTWTDMVADFQANGWSSSDLVVFTYDSHQSNVTTANQ
LAARISSVRSATGAAKVDLVTHSMGALSSRYYLKNLGGTSVVDDFVSLGGPNHGTSTATLCGWLYTACSEMSPGSSFLNT
LNSGDETPGAVNYGTWWSSCDDTINPDSSVSLSGATNTAAGCLGHSELHDDNTVASQVRAFIG
>Mature_222_residues
SRAARILGALAVAAAALLGTSTAASAATHEPVIFVYGFNGSTSTWTDMVADFQANGWSSSDLVVFTYDSHQSNVTTANQL
AARISSVRSATGAAKVDLVTHSMGALSSRYYLKNLGGTSVVDDFVSLGGPNHGTSTATLCGWLYTACSEMSPGSSFLNTL
NSGDETPGAVNYGTWWSSCDDTINPDSSVSLSGATNTAAGCLGHSELHDDNTVASQVRAFIG

Specific function: An esterase which preferentially hydrolyzes triacylglyceride substrates with short chain fatty acids (less than C10) with the maximum activity towards tricaprylin (C8:0). Active against p-nitrophenylesters with fatty acid chain lengths from C6 to C18 [H]

COG id: COG1075

COG function: function code R; Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002918 [H]

Pfam domain/function: PF01674 Lipase_2 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 22951; Mature: 22820

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAARILGALAVAAAALLGTSTAASAATHEPVIFVYGFNGSTSTWTDMVADFQANGWSS
CCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCC
SDLVVFTYDSHQSNVTTANQLAARISSVRSATGAAKVDLVTHSMGALSSRYYLKNLGGTS
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCHH
VVDDFVSLGGPNHGTSTATLCGWLYTACSEMSPGSSFLNTLNSGDETPGAVNYGTWWSSC
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
DDTINPDSSVSLSGATNTAAGCLGHSELHDDNTVASQVRAFIG
CCCCCCCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SRAARILGALAVAAAALLGTSTAASAATHEPVIFVYGFNGSTSTWTDMVADFQANGWSS
CHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCC
SDLVVFTYDSHQSNVTTANQLAARISSVRSATGAAKVDLVTHSMGALSSRYYLKNLGGTS
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCHH
VVDDFVSLGGPNHGTSTATLCGWLYTACSEMSPGSSFLNTLNSGDETPGAVNYGTWWSSC
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
DDTINPDSSVSLSGATNTAAGCLGHSELHDDNTVASQVRAFIG
CCCCCCCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]