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The map label for this gene is phoB [H]

Identifier: 157960817

GI number: 157960817

Start: 1201053

End: 1202495

Strand: Reverse

Name: phoB [H]

Synonym: Spea_0989

Alternate gene names: 157960817

Gene position: 1202495-1201053 (Counterclockwise)

Preceding gene: 157960818

Following gene: 157960816

Centisome position: 23.24

GC content: 49.0

Gene sequence:

>1443_bases
ATGACTCACTTAACCTCGATGGATCGCTTCCCTGCAATCTGCTCAAGTCTAATTATCGCAAGCCTATGCAGCACAGCCTT
ATGTAGCACAGTTCTGTTAAGTAAAGAGGCTGCAGCAGACACAGACGATCCCTTTGCGCCAATTATTGCCAACGAATACC
TACCGAAAAATATCATCTACCTGATTGGTGACGGTATGGGGCCAGCTTATACCAGCGCCTACCGCTACTACAGCGATAAC
CCGCAAACTCAAAGAGTCGAGAAGACCATTTTCGACAAATTGTTAGTCGGCATGTCGAGCACCTACCCTGATGACGATAC
TTATGTCACCGACTCTGCGGCGGCGGCTACCGCGCTCGCGACCAGCTATAAGAGCTATAACGGCGCCATATCAGTAGACC
ATCACGGCGGGTCTTTCCCCACACTGCTGGAGATGGCGAAAGCGCAAGGAAAAACGACAGCGGTGGTGGTAACGTCACAG
ATTAACCACGCGACCCCTGCCAGCTTTTTAGCCCATAACGAAAGCCGCCGAAACTACGACCAGATTGCCGATAGCTATTT
AACTAACCTTATCAACAAGCGTCCCGTTGCCGATTTGATGCTGGGCGGCGGTAGCCAATACTTCGCACGTGATGACCGAG
ATCTGACCTTAGAATTTCAAGAGTATGGCTACCAGTACGTCGATAGCCTAAGCGATTTAAACAGCATCGAAAAACTGCCA
GCCCTAGGCCTATTCGCCCCTAAAGGCTTACCTAGCACATTGGAGAGCCAAGACCCTCTTCACCTCACCACTATGACCCG
AAAAGCCTTGGCTTTATTATCAGAGCAAGACGCCCCCTTTGTGATGATGGTAGAGGCCAGTCAAATTGACTGGTGTGGAC
ACAGCAATGATATTGTGTGCGCCATGAATGAAATGCACGACTTTGCTAATACTTTGAGCCTAGTAAAGCACTATGTCGAT
ACCCACCCAGACACCTTACTCATTGCAACGGCTGATCACTCAACGGGTGGCTTAACCTTAGGACGAGAAGGCACTTACCA
ATGGCAAGGCCAGTTACTGCATCAAGTTCACTCGCTGCCGAGTTCCATCGCAAAACAAATTGTTGAGACACCAGAGGTCC
TCAATGATTTACCTAGCTTCACTCACTTTATTCGCCCCCATATCAGCATAGATATTGAGCCCAAGAAGCTCGACTTCCTA
CGCAGCCGGCTTAATAGTCGCCTGTCTCGACACGAACCCACAAGAGAGATGGCAGACATTCTTAAGCAAGCAATTGATAG
GTTCACCTATACTGGATGGACTACTGGAGGTCATGATGCTATCGATGTTCCGGTTTACGCCTATGGTAAAGGTGCAGTGT
ACTTTCGAGGTCATAAAGACAATACCGAAATAGCCGCCACCTTGATTCAGATGGTGCAAGACGATCGCTACAAGGTACAG
TAA

Upstream 100 bases:

>100_bases
CTGTACCTAAAAAATCCAACACTCAGCAGAGGCAGCGCTCACTTTGAACCTGGCCAGCCAGACAAAACACAAGTAACATT
CCATTAAGGATAACTAGAAC

Downstream 100 bases:

>100_bases
CACTGCTCCTCATTACACTTGGTTGTAGCAACAATAAATTTCATTTTGGAACTGTTTACTTCGTAATTTAGCAAAACATA
AAGTACAGTCGACAGTTACT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTHLTSMDRFPAICSSLIIASLCSTALCSTVLLSKEAAADTDDPFAPIIANEYLPKNIIYLIGDGMGPAYTSAYRYYSDN
PQTQRVEKTIFDKLLVGMSSTYPDDDTYVTDSAAAATALATSYKSYNGAISVDHHGGSFPTLLEMAKAQGKTTAVVVTSQ
INHATPASFLAHNESRRNYDQIADSYLTNLINKRPVADLMLGGGSQYFARDDRDLTLEFQEYGYQYVDSLSDLNSIEKLP
ALGLFAPKGLPSTLESQDPLHLTTMTRKALALLSEQDAPFVMMVEASQIDWCGHSNDIVCAMNEMHDFANTLSLVKHYVD
THPDTLLIATADHSTGGLTLGREGTYQWQGQLLHQVHSLPSSIAKQIVETPEVLNDLPSFTHFIRPHISIDIEPKKLDFL
RSRLNSRLSRHEPTREMADILKQAIDRFTYTGWTTGGHDAIDVPVYAYGKGAVYFRGHKDNTEIAATLIQMVQDDRYKVQ

Sequences:

>Translated_480_residues
MTHLTSMDRFPAICSSLIIASLCSTALCSTVLLSKEAAADTDDPFAPIIANEYLPKNIIYLIGDGMGPAYTSAYRYYSDN
PQTQRVEKTIFDKLLVGMSSTYPDDDTYVTDSAAAATALATSYKSYNGAISVDHHGGSFPTLLEMAKAQGKTTAVVVTSQ
INHATPASFLAHNESRRNYDQIADSYLTNLINKRPVADLMLGGGSQYFARDDRDLTLEFQEYGYQYVDSLSDLNSIEKLP
ALGLFAPKGLPSTLESQDPLHLTTMTRKALALLSEQDAPFVMMVEASQIDWCGHSNDIVCAMNEMHDFANTLSLVKHYVD
THPDTLLIATADHSTGGLTLGREGTYQWQGQLLHQVHSLPSSIAKQIVETPEVLNDLPSFTHFIRPHISIDIEPKKLDFL
RSRLNSRLSRHEPTREMADILKQAIDRFTYTGWTTGGHDAIDVPVYAYGKGAVYFRGHKDNTEIAATLIQMVQDDRYKVQ
>Mature_479_residues
THLTSMDRFPAICSSLIIASLCSTALCSTVLLSKEAAADTDDPFAPIIANEYLPKNIIYLIGDGMGPAYTSAYRYYSDNP
QTQRVEKTIFDKLLVGMSSTYPDDDTYVTDSAAAATALATSYKSYNGAISVDHHGGSFPTLLEMAKAQGKTTAVVVTSQI
NHATPASFLAHNESRRNYDQIADSYLTNLINKRPVADLMLGGGSQYFARDDRDLTLEFQEYGYQYVDSLSDLNSIEKLPA
LGLFAPKGLPSTLESQDPLHLTTMTRKALALLSEQDAPFVMMVEASQIDWCGHSNDIVCAMNEMHDFANTLSLVKHYVDT
HPDTLLIATADHSTGGLTLGREGTYQWQGQLLHQVHSLPSSIAKQIVETPEVLNDLPSFTHFIRPHISIDIEPKKLDFLR
SRLNSRLSRHEPTREMADILKQAIDRFTYTGWTTGGHDAIDVPVYAYGKGAVYFRGHKDNTEIAATLIQMVQDDRYKVQ

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=339, Percent_Identity=27.1386430678466, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI94721246, Length=329, Percent_Identity=27.0516717325228, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI157266296, Length=329, Percent_Identity=27.0516717325228, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI294660770, Length=329, Percent_Identity=26.7477203647416, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI157266292, Length=360, Percent_Identity=27.2222222222222, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI294660772, Length=276, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI48994877, Length=336, Percent_Identity=34.5238095238095, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320689, Length=499, Percent_Identity=32.064128256513, Blast_Score=180, Evalue=4e-46,
Organism=Drosophila melanogaster, GI21355149, Length=339, Percent_Identity=28.023598820059, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI18859923, Length=317, Percent_Identity=29.0220820189274, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24651554, Length=339, Percent_Identity=30.3834808259587, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355981, Length=333, Percent_Identity=27.9279279279279, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355151, Length=335, Percent_Identity=27.4626865671642, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI85725200, Length=339, Percent_Identity=27.4336283185841, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI85815833, Length=339, Percent_Identity=27.4336283185841, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 53123; Mature: 52992

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLTSMDRFPAICSSLIIASLCSTALCSTVLLSKEAAADTDDPFAPIIANEYLPKNIIY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEE
LIGDGMGPAYTSAYRYYSDNPQTQRVEKTIFDKLLVGMSSTYPDDDTYVTDSAAAATALA
EEECCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHH
TSYKSYNGAISVDHHGGSFPTLLEMAKAQGKTTAVVVTSQINHATPASFLAHNESRRNYD
HHHHCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCCCCCHH
QIADSYLTNLINKRPVADLMLGGGSQYFARDDRDLTLEFQEYGYQYVDSLSDLNSIEKLP
HHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHCC
ALGLFAPKGLPSTLESQDPLHLTTMTRKALALLSEQDAPFVMMVEASQIDWCGHSNDIVC
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEE
AMNEMHDFANTLSLVKHYVDTHPDTLLIATADHSTGGLTLGREGTYQWQGQLLHQVHSLP
EHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHH
SSIAKQIVETPEVLNDLPSFTHFIRPHISIDIEPKKLDFLRSRLNSRLSRHEPTREMADI
HHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKQAIDRFTYTGWTTGGHDAIDVPVYAYGKGAVYFRGHKDNTEIAATLIQMVQDDRYKVQ
HHHHHHHHEECCCCCCCCCCEECCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
THLTSMDRFPAICSSLIIASLCSTALCSTVLLSKEAAADTDDPFAPIIANEYLPKNIIY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEE
LIGDGMGPAYTSAYRYYSDNPQTQRVEKTIFDKLLVGMSSTYPDDDTYVTDSAAAATALA
EEECCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHH
TSYKSYNGAISVDHHGGSFPTLLEMAKAQGKTTAVVVTSQINHATPASFLAHNESRRNYD
HHHHCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCCCCCHH
QIADSYLTNLINKRPVADLMLGGGSQYFARDDRDLTLEFQEYGYQYVDSLSDLNSIEKLP
HHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHCC
ALGLFAPKGLPSTLESQDPLHLTTMTRKALALLSEQDAPFVMMVEASQIDWCGHSNDIVC
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEE
AMNEMHDFANTLSLVKHYVDTHPDTLLIATADHSTGGLTLGREGTYQWQGQLLHQVHSLP
EHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHH
SSIAKQIVETPEVLNDLPSFTHFIRPHISIDIEPKKLDFLRSRLNSRLSRHEPTREMADI
HHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKQAIDRFTYTGWTTGGHDAIDVPVYAYGKGAVYFRGHKDNTEIAATLIQMVQDDRYKVQ
HHHHHHHHEECCCCCCCCCCEECCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]