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The map label for this gene is lip2 [H]

Identifier: 157371092

GI number: 157371092

Start: 3130886

End: 3131860

Strand: Direct

Name: lip2 [H]

Synonym: Spro_2852

Alternate gene names: 157371092

Gene position: 3130886-3131860 (Clockwise)

Preceding gene: 157371090

Following gene: 157371099

Centisome position: 57.46

GC content: 58.77

Gene sequence:

>975_bases
ATGTCCAAGAAAACTGTAGATCCCACCCAACCGGCCGCACAGCATGATATCCGCACCTTTCTGGATGCGTTGAATGCCGG
CGGTGGCAAGCCGATGGAACAAATGAAACCGAAAGAGGCGCGCAAAGTGCTGGAGGGCGCGCAGCGTAGCGTCGAAGTGC
CACTGCACGAGGTCGAAATCAGCGAGAAAACCATTCATGTCGAGGGGCAGGATATCCTGCTGCACATTGTGCGACCGGCC
AAGGTCAAGGAAACACTGCCGGTATTTATGTTCTTCCACGGCGGTGGCTGGGTGCTGGGCGACTTCCCTACCCATGAGCG
GCTGGTGCGTGACTTGGTTTACAGCTCAGGTGCGGCAGCGGTGTTCGTCAACTATACCCCGTCACCGGAGGCTCATTACC
CAACCGCCATCAATCAGGCCTATGCCGCCACCGAATGGGTGGCCGAATACGGCGAGAAAATCAACGTTGACGGCTCGCGG
CTGGCAGTGGTCGGCAACAGCGTCGGCGGTAATATGGCGGCGGTGGTCAGCCTGATGGCCAAAGAAAAAGGCACCCCGGC
GCTGCGCACGCAAATCCTGCTGTGGCCGGTAACGCACGCCAGCTTTGATACCGACTCTTACCACCAATTCGCCGAAGGCC
ACTTCCTGACCCGCAACATGATGAAATGGTTCTGGGACAACTACACCACCGATAAAGCGCAGCGTAAGGAGATCTACGCC
TCACCGCTCAATGCCACGCTGGAACAGCTAAAAGGCCTGCCGCCGGCACTGGTGCAGACCGCCGAACTGGACGTGCTGCG
TGACGAGGGTGAAGCCTATGCCCGGCTGCTGAATGCCGCCGGGGTAGAGGTCACTTCCACTCGCTACAATGGACTGATCC
ACGATTACGGTCTGCTAAACCCTATCGCTCATGTGCCGGCGGTGCATTCCGCCATTCATCAGGCCGGGCGGGCGCTCAAA
CATTATCTGGCCTAG

Upstream 100 bases:

>100_bases
CGTGGCTTGGCCCGCCTTTTCTCAACGTGCTGTCGGGCCAGCAAACGGCAGCCATTCAACTACACTGTGATCTCGACCGA
AAAACGCTTGAGGAGCCATG

Downstream 100 bases:

>100_bases
GCTAATCGGCATAATGGCAGGCTACCCGATGCCCTGCTGACACCGTCCGTAATGCCGGGGCCTGTTCCCGGCACTGCGCG
GTGGCCTGCGGACAACGGGT

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSKKTVDPTQPAAQHDIRTFLDALNAGGGKPMEQMKPKEARKVLEGAQRSVEVPLHEVEISEKTIHVEGQDILLHIVRPA
KVKETLPVFMFFHGGGWVLGDFPTHERLVRDLVYSSGAAAVFVNYTPSPEAHYPTAINQAYAATEWVAEYGEKINVDGSR
LAVVGNSVGGNMAAVVSLMAKEKGTPALRTQILLWPVTHASFDTDSYHQFAEGHFLTRNMMKWFWDNYTTDKAQRKEIYA
SPLNATLEQLKGLPPALVQTAELDVLRDEGEAYARLLNAAGVEVTSTRYNGLIHDYGLLNPIAHVPAVHSAIHQAGRALK
HYLA

Sequences:

>Translated_324_residues
MSKKTVDPTQPAAQHDIRTFLDALNAGGGKPMEQMKPKEARKVLEGAQRSVEVPLHEVEISEKTIHVEGQDILLHIVRPA
KVKETLPVFMFFHGGGWVLGDFPTHERLVRDLVYSSGAAAVFVNYTPSPEAHYPTAINQAYAATEWVAEYGEKINVDGSR
LAVVGNSVGGNMAAVVSLMAKEKGTPALRTQILLWPVTHASFDTDSYHQFAEGHFLTRNMMKWFWDNYTTDKAQRKEIYA
SPLNATLEQLKGLPPALVQTAELDVLRDEGEAYARLLNAAGVEVTSTRYNGLIHDYGLLNPIAHVPAVHSAIHQAGRALK
HYLA
>Mature_323_residues
SKKTVDPTQPAAQHDIRTFLDALNAGGGKPMEQMKPKEARKVLEGAQRSVEVPLHEVEISEKTIHVEGQDILLHIVRPAK
VKETLPVFMFFHGGGWVLGDFPTHERLVRDLVYSSGAAAVFVNYTPSPEAHYPTAINQAYAATEWVAEYGEKINVDGSRL
AVVGNSVGGNMAAVVSLMAKEKGTPALRTQILLWPVTHASFDTDSYHQFAEGHFLTRNMMKWFWDNYTTDKAQRKEIYAS
PLNATLEQLKGLPPALVQTAELDVLRDEGEAYARLLNAAGVEVTSTRYNGLIHDYGLLNPIAHVPAVHSAIHQAGRALKH
YLA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=282, Percent_Identity=26.9503546099291, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI21328446, Length=139, Percent_Identity=28.7769784172662, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI157041239, Length=144, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI68051721, Length=158, Percent_Identity=29.746835443038, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786682, Length=213, Percent_Identity=26.7605633802817, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17567059, Length=266, Percent_Identity=26.6917293233083, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71996133, Length=159, Percent_Identity=30.8176100628931, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=124, Percent_Identity=31.4516129032258, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17540028, Length=139, Percent_Identity=30.9352517985612, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 35741; Mature: 35610

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKTVDPTQPAAQHDIRTFLDALNAGGGKPMEQMKPKEARKVLEGAQRSVEVPLHEVEI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHEEE
SEKTIHVEGQDILLHIVRPAKVKETLPVFMFFHGGGWVLGDFPTHERLVRDLVYSSGAAA
CCCEEEECCCEEEEEEECCHHHHHCCEEEEEEECCCEEECCCCCHHHHHHHHHHCCCCCE
VFVNYTPSPEAHYPTAINQAYAATEWVAEYGEKINVDGSRLAVVGNSVGGNMAAVVSLMA
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCEEEEEECCCCCHHHHHHHHHH
KEKGTPALRTQILLWPVTHASFDTDSYHQFAEGHFLTRNMMKWFWDNYTTDKAQRKEIYA
HCCCCHHHHEEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
SPLNATLEQLKGLPPALVQTAELDVLRDEGEAYARLLNAAGVEVTSTRYNGLIHDYGLLN
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEECHHHCCCCHHHHHHH
PIAHVPAVHSAIHQAGRALKHYLA
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKKTVDPTQPAAQHDIRTFLDALNAGGGKPMEQMKPKEARKVLEGAQRSVEVPLHEVEI
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHEEE
SEKTIHVEGQDILLHIVRPAKVKETLPVFMFFHGGGWVLGDFPTHERLVRDLVYSSGAAA
CCCEEEECCCEEEEEEECCHHHHHCCEEEEEEECCCEEECCCCCHHHHHHHHHHCCCCCE
VFVNYTPSPEAHYPTAINQAYAATEWVAEYGEKINVDGSRLAVVGNSVGGNMAAVVSLMA
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCEEEEEECCCCCHHHHHHHHHH
KEKGTPALRTQILLWPVTHASFDTDSYHQFAEGHFLTRNMMKWFWDNYTTDKAQRKEIYA
HCCCCHHHHEEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
SPLNATLEQLKGLPPALVQTAELDVLRDEGEAYARLLNAAGVEVTSTRYNGLIHDYGLLN
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEECHHHCCCCHHHHHHH
PIAHVPAVHSAIHQAGRALKHYLA
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]