Click here to switch to the map view.

The map label for this gene is mlc [H]

Identifier: 157370523

GI number: 157370523

Start: 2486532

End: 2487749

Strand: Direct

Name: mlc [H]

Synonym: Spro_2283

Alternate gene names: 157370523

Gene position: 2486532-2487749 (Clockwise)

Preceding gene: 157370522

Following gene: 157370524

Centisome position: 45.63

GC content: 57.39

Gene sequence:

>1218_bases
GTGATTGCTGTTGGGCAGCCTGGGCATATCGATCAAATCAAGCAAACAAACGCAGGGGCAGTTTATCGGCTAATCGATCA
ACTGGGCCCAATTTCGCGCATCGAACTGTCAAAACGGGCGCAGCTCGCCCCCGCCAGCATCACCAAAATCGTACGCGAAC
TGCTGGAAGCCCATCTGGTTAAAGAAACCGAGTTCCAGGAAGTTGGCAGCCGTGGCCGTCCGGCGGTTGGGCTGGTGCTG
GACACCGAAGCTTGGCACTACCTTTCCGCACGCATCAGCCGTGGCACCATTACGCTGGCGCTGCGCGATATCAGCAGCAA
ACTGGTGGTGGAAGAGTTGTTGCCACTGGCTGCTGAACATCCCGATCCATTGTTAAAACGCATTCTTGCTGAAGTCGACC
AGTTCTTTATCCGCCATCAAAGCAAGCTTGAACGCCTGACGGCGATCGCCATTACGCTGCCGGGCATGGTTGACGCGATT
TCCGGCGTGGTACACCGCATGCCGTTTTACGATGTCGAGGATATGCCGTTAGGCCCGGCGCTGGAAACGCGTACCGGTCT
GCCGGTTTACCTGCAGCACGACATCAACGCCTGGACCATGGCCGAAGCCTTATACGGTGCATCGCGCGGCAGCCAAAACG
TGATTCAGGTGGTGATCGACCACAACGTTGGCGCTGGGGTGATCACCGGTGGCAGGGTGCTGCATGCCGGCAGCCGTAGC
GTGGTGGAGATTGGCCATACTCAGGTCGATCCTTACGGCAAACGTTGTTACTGCGGCAATCACGGTTGCCTGGAAACCGT
CGCCAGCATCGAGAATATGCTGGAAATTGCCCGTTCGCGCCTCGGTACATCAATGAGTTCCAGTCTGCACGGTGCACCGC
TGACGGTCGAGTCGCTGTGTGATGCGGCGCTGGCGGGTGACCAGTTGGCGAAAGACATTATCCTTGGGGTAGGCCACAGC
GTGGGCCGCATTCTGGCGATCATGGTTAATCTGTTCAATCCGGAAAAAATCCTGGTCGGTTCCCCGCTGAACCGCGCAGC
AGAAATTCTGCATCCGGCCATTGCCTCCTGTATACGTCAGCAGTCGCTGCCGGCCTACAGTGAGCAGGTGCAGGTGGAGT
CCACTCAGTTCTTCAATAAGGGCACTATGCCCGGCGCGGCACTGGTGAAAGAAGCGTTATACAACGGTTCGCTGCTGGTG
CAGTTGCTGCAAGGCTAA

Upstream 100 bases:

>100_bases
AAATCACATAACGAATCAAATAGTTGACGCTGTATTACAAAAGCCCCACCATAGCCGATAGTCTTTATTTAGAAGCGCGA
AAAATAGTAGGAGCAGGTTT

Downstream 100 bases:

>100_bases
TACCAATCAGCAATTCTTATCGTTTAACGACAAAAGGTTGAGCTACCTCAAGCCCCGTCTCCCCGGCATCCTCTAGACTT
TTCCCGTTCGTCGCTTTCGC

Product: ROK family protein

Products: NA

Alternate protein names: Making large colonies protein [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MIAVGQPGHIDQIKQTNAGAVYRLIDQLGPISRIELSKRAQLAPASITKIVRELLEAHLVKETEFQEVGSRGRPAVGLVL
DTEAWHYLSARISRGTITLALRDISSKLVVEELLPLAAEHPDPLLKRILAEVDQFFIRHQSKLERLTAIAITLPGMVDAI
SGVVHRMPFYDVEDMPLGPALETRTGLPVYLQHDINAWTMAEALYGASRGSQNVIQVVIDHNVGAGVITGGRVLHAGSRS
VVEIGHTQVDPYGKRCYCGNHGCLETVASIENMLEIARSRLGTSMSSSLHGAPLTVESLCDAALAGDQLAKDIILGVGHS
VGRILAIMVNLFNPEKILVGSPLNRAAEILHPAIASCIRQQSLPAYSEQVQVESTQFFNKGTMPGAALVKEALYNGSLLV
QLLQG

Sequences:

>Translated_405_residues
MIAVGQPGHIDQIKQTNAGAVYRLIDQLGPISRIELSKRAQLAPASITKIVRELLEAHLVKETEFQEVGSRGRPAVGLVL
DTEAWHYLSARISRGTITLALRDISSKLVVEELLPLAAEHPDPLLKRILAEVDQFFIRHQSKLERLTAIAITLPGMVDAI
SGVVHRMPFYDVEDMPLGPALETRTGLPVYLQHDINAWTMAEALYGASRGSQNVIQVVIDHNVGAGVITGGRVLHAGSRS
VVEIGHTQVDPYGKRCYCGNHGCLETVASIENMLEIARSRLGTSMSSSLHGAPLTVESLCDAALAGDQLAKDIILGVGHS
VGRILAIMVNLFNPEKILVGSPLNRAAEILHPAIASCIRQQSLPAYSEQVQVESTQFFNKGTMPGAALVKEALYNGSLLV
QLLQG
>Mature_405_residues
MIAVGQPGHIDQIKQTNAGAVYRLIDQLGPISRIELSKRAQLAPASITKIVRELLEAHLVKETEFQEVGSRGRPAVGLVL
DTEAWHYLSARISRGTITLALRDISSKLVVEELLPLAAEHPDPLLKRILAEVDQFFIRHQSKLERLTAIAITLPGMVDAI
SGVVHRMPFYDVEDMPLGPALETRTGLPVYLQHDINAWTMAEALYGASRGSQNVIQVVIDHNVGAGVITGGRVLHAGSRS
VVEIGHTQVDPYGKRCYCGNHGCLETVASIENMLEIARSRLGTSMSSSLHGAPLTVESLCDAALAGDQLAKDIILGVGHS
VGRILAIMVNLFNPEKILVGSPLNRAAEILHPAIASCIRQQSLPAYSEQVQVESTQFFNKGTMPGAALVKEALYNGSLLV
QLLQG

Specific function: Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI1787878, Length=406, Percent_Identity=75.3694581280788, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1786891, Length=399, Percent_Identity=40.8521303258145, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI87082127, Length=372, Percent_Identity=23.1182795698925, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081733, Length=278, Percent_Identity=25.5395683453237, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: NA

Molecular weight: Translated: 43718; Mature: 43718

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVGQPGHIDQIKQTNAGAVYRLIDQLGPISRIELSKRAQLAPASITKIVRELLEAHLV
CCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KETEFQEVGSRGRPAVGLVLDTEAWHYLSARISRGTITLALRDISSKLVVEELLPLAAEH
CHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCC
PDPLLKRILAEVDQFFIRHQSKLERLTAIAITLPGMVDAISGVVHRMPFYDVEDMPLGPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
LETRTGLPVYLQHDINAWTMAEALYGASRGSQNVIQVVIDHNVGAGVITGGRVLHAGSRS
CCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCEEEECCCCC
VVEIGHTQVDPYGKRCYCGNHGCLETVASIENMLEIARSRLGTSMSSSLHGAPLTVESLC
EEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHH
DAALAGDQLAKDIILGVGHSVGRILAIMVNLFNPEKILVGSPLNRAAEILHPAIASCIRQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHH
QSLPAYSEQVQVESTQFFNKGTMPGAALVKEALYNGSLLVQLLQG
HCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MIAVGQPGHIDQIKQTNAGAVYRLIDQLGPISRIELSKRAQLAPASITKIVRELLEAHLV
CCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KETEFQEVGSRGRPAVGLVLDTEAWHYLSARISRGTITLALRDISSKLVVEELLPLAAEH
CHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCC
PDPLLKRILAEVDQFFIRHQSKLERLTAIAITLPGMVDAISGVVHRMPFYDVEDMPLGPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
LETRTGLPVYLQHDINAWTMAEALYGASRGSQNVIQVVIDHNVGAGVITGGRVLHAGSRS
CCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCEEEECCCCC
VVEIGHTQVDPYGKRCYCGNHGCLETVASIENMLEIARSRLGTSMSSSLHGAPLTVESLC
EEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHH
DAALAGDQLAKDIILGVGHSVGRILAIMVNLFNPEKILVGSPLNRAAEILHPAIASCIRQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHH
QSLPAYSEQVQVESTQFFNKGTMPGAALVKEALYNGSLLVQLLQG
HCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766024; 9097039; 9278503 [H]