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The map label for this gene is ynfL [H]

Identifier: 157370522

GI number: 157370522

Start: 2485484

End: 2486392

Strand: Direct

Name: ynfL [H]

Synonym: Spro_2282

Alternate gene names: 157370522

Gene position: 2485484-2486392 (Clockwise)

Preceding gene: 157370519

Following gene: 157370523

Centisome position: 45.61

GC content: 56.33

Gene sequence:

>909_bases
ATGAATATCGAATTGAGGCATCTGCGTTATTTTGTCGCCGTGGCAGAAGAGTTGCATTTTGGTCGGGCCGCAGAACGTCT
GCGTATCTCTCAGCCGCCGCTTAGCCAGCAAATCCAGGCGCTGGAGGAAATGGTCGGCGCACGTCTGCTGGCGCGTAACA
ACCGCAATGTCAGCCTGACGCAGGCGGGAGACATGTTTCTGAAAGAGGCCTATCAGGTGCTTGATCAGGTGAGTCGCGCT
GCCGAGAAAGCGGCGCGGTTGGAACGTGGCGAACTGGGTGAGTTGACCATAGGTTTCACCTCTTCGGCCCCCTTTATCAG
CGTGGTATCGCGCAGCCTGCGAGCTTTTCGTCAGCAGCACTCACAGGTGCACATCAAGATGCGCGAAGTGAATACCAAGC
AGCAGATCGAACCCTTACTTAATGGCGAGCTGGATCTGGGCGTGATGCGCAACACGCGCTTACCGGACGTGTTAAAGCAT
CAGCTTTTACTGTGTGAACCCTTGGTGGCGGTGGTACCGGAAGGGCATCCGTTAACCCATGTGGCACCCGGTACGCTGCG
CTTCCAACATCTGGCGGAAGAACCCTTTGTCTTTTTCTCGCGTGAAGTCGGAACCGCGCTGCATGACGAGATCTTGTCGC
TGTTGGCCAAAGCGGGGATCACCCCTTATATCACCCAGGAAGTCGGAGAGGCGATGACCATTATCGGCCTGGTCTCCTCC
GGCTTGGGCATCTCGATTCTGCCGACGTCTTTCGCGCGGGTTAAAATCGACGGCGTGCAATATTTACCGCTGGCGGAGCC
TGAGGCGATGACCGAGGTTTGGTTGGTACATCATCGCCAACGCCCTCTGACGGCAGCGGCCAAATCTCTGATGGCGTTAC
TGTTGCCTGAAAGCCAACTGGGCAAATAA

Upstream 100 bases:

>100_bases
AACTAGGGTATATATCAACACATTATGAGTTAACAAAATCATAGGGAGGGGCCAGTTTTTTGTCTAATATATTAATAATT
AAAATTGAGACGTTTAAAGT

Downstream 100 bases:

>100_bases
AACGCATTCCGCAGGGAAAGTGGGGTGAATTTGTGCAGCAAATCACATAACGAATCAAATAGTTGACGCTGTATTACAAA
AGCCCCACCATAGCCGATAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MNIELRHLRYFVAVAEELHFGRAAERLRISQPPLSQQIQALEEMVGARLLARNNRNVSLTQAGDMFLKEAYQVLDQVSRA
AEKAARLERGELGELTIGFTSSAPFISVVSRSLRAFRQQHSQVHIKMREVNTKQQIEPLLNGELDLGVMRNTRLPDVLKH
QLLLCEPLVAVVPEGHPLTHVAPGTLRFQHLAEEPFVFFSREVGTALHDEILSLLAKAGITPYITQEVGEAMTIIGLVSS
GLGISILPTSFARVKIDGVQYLPLAEPEAMTEVWLVHHRQRPLTAAAKSLMALLLPESQLGK

Sequences:

>Translated_302_residues
MNIELRHLRYFVAVAEELHFGRAAERLRISQPPLSQQIQALEEMVGARLLARNNRNVSLTQAGDMFLKEAYQVLDQVSRA
AEKAARLERGELGELTIGFTSSAPFISVVSRSLRAFRQQHSQVHIKMREVNTKQQIEPLLNGELDLGVMRNTRLPDVLKH
QLLLCEPLVAVVPEGHPLTHVAPGTLRFQHLAEEPFVFFSREVGTALHDEILSLLAKAGITPYITQEVGEAMTIIGLVSS
GLGISILPTSFARVKIDGVQYLPLAEPEAMTEVWLVHHRQRPLTAAAKSLMALLLPESQLGK
>Mature_302_residues
MNIELRHLRYFVAVAEELHFGRAAERLRISQPPLSQQIQALEEMVGARLLARNNRNVSLTQAGDMFLKEAYQVLDQVSRA
AEKAARLERGELGELTIGFTSSAPFISVVSRSLRAFRQQHSQVHIKMREVNTKQQIEPLLNGELDLGVMRNTRLPDVLKH
QLLLCEPLVAVVPEGHPLTHVAPGTLRFQHLAEEPFVFFSREVGTALHDEILSLLAKAGITPYITQEVGEAMTIIGLVSS
GLGISILPTSFARVKIDGVQYLPLAEPEAMTEVWLVHHRQRPLTAAAKSLMALLLPESQLGK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=285, Percent_Identity=60.3508771929825, Blast_Score=344, Evalue=4e-96,
Organism=Escherichia coli, GI1788887, Length=263, Percent_Identity=36.8821292775665, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI2367136, Length=291, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1790399, Length=293, Percent_Identity=30.3754266211604, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI157672245, Length=296, Percent_Identity=30.0675675675676, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081904, Length=178, Percent_Identity=34.8314606741573, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693105, Length=296, Percent_Identity=26.6891891891892, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787601, Length=301, Percent_Identity=28.9036544850498, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788748, Length=307, Percent_Identity=25.7328990228013, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=28.8537549407115, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=29.8804780876494, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789506, Length=286, Percent_Identity=24.1258741258741, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788481, Length=302, Percent_Identity=25.4966887417219, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787530, Length=270, Percent_Identity=24.0740740740741, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790262, Length=250, Percent_Identity=28, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789173, Length=172, Percent_Identity=28.4883720930233, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI48994962, Length=259, Percent_Identity=27.7992277992278, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788296, Length=245, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33561; Mature: 33561

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAERLRISQPPLSQQIQALEEMVGARLLARNNRNVSLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
QAGDMFLKEAYQVLDQVSRAAEKAARLERGELGELTIGFTSSAPFISVVSRSLRAFRQQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
SQVHIKMREVNTKQQIEPLLNGELDLGVMRNTRLPDVLKHQLLLCEPLVAVVPEGHPLTH
HHEEEEEECCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
VAPGTLRFQHLAEEPFVFFSREVGTALHDEILSLLAKAGITPYITQEVGEAMTIIGLVSS
ECCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GLGISILPTSFARVKIDGVQYLPLAEPEAMTEVWLVHHRQRPLTAAAKSLMALLLPESQL
CCCEEEECCCCEEEEECCEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHC
GK
CC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAERLRISQPPLSQQIQALEEMVGARLLARNNRNVSLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
QAGDMFLKEAYQVLDQVSRAAEKAARLERGELGELTIGFTSSAPFISVVSRSLRAFRQQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
SQVHIKMREVNTKQQIEPLLNGELDLGVMRNTRLPDVLKHQLLLCEPLVAVVPEGHPLTH
HHEEEEEECCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
VAPGTLRFQHLAEEPFVFFSREVGTALHDEILSLLAKAGITPYITQEVGEAMTIIGLVSS
ECCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GLGISILPTSFARVKIDGVQYLPLAEPEAMTEVWLVHHRQRPLTAAAKSLMALLLPESQL
CCCEEEECCCCEEEEECCEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHC
GK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]