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The map label for this gene is bioD2 [H]

Identifier: 157370524

GI number: 157370524

Start: 2487901

End: 2488572

Strand: Direct

Name: bioD2 [H]

Synonym: Spro_2284

Alternate gene names: 157370524

Gene position: 2487901-2488572 (Clockwise)

Preceding gene: 157370523

Following gene: 157370526

Centisome position: 45.66

GC content: 54.46

Gene sequence:

>672_bases
ATGTTAACGCGTTTATTTGTTACAGGTACGGATACCGATGTCGGTAAAACCGTGGTTTCTCGCGCTTTATTACAGGCGCT
TGCTGCGCAGGGCCGTTCTGTGGCCGGCTATAAACCTGTCGCTGCGCGCTGTCAGGAAACCAGCGAAGGCCTTCGCAATA
AAGACGCTTTGGTTTTGCAGGCATCTTCGACCATGCCGCTGTCGTATCAGGAAATTAATCCCATTACCTGTCTGGAAGAA
GTCTTTCACGCGCAACCTACCAAGGAAACCAACTACGGTGTGATGAGCAGTGGGCTGCAACATTTGTCCACCAAAGCCGA
TACCGTGGTGGTGGAAGGGAGCGGCGGTTGGCGCGTGTTGATGAACGATATGCGGCCTTACGCCGAATGGGTCATTCAGG
AACAACTGCCGGTGGTTTTGGTGGTGGGGATCAAACTGGGCTGTGTCAGCCACGCTTTATTAACGGCGCAGGCGATCATT
AATGACGGCTTGCCGCTGCTGGGCTGGGTCGCGAACCGCATCAATCCGGGGCTGGCACATTACGCCGAAACTATCGATGC
GATGCAGCAACGCATTCCTGCGCCGCTGCTGGGTGAAATACCTTATCTGCCACGCGCCGAACAGCGGGAACTGGCACGTT
ATCTGGATATCTCTCCACTGCTAAACCGTTAG

Upstream 100 bases:

>100_bases
GCCCCGTCTCCCCGGCATCCTCTAGACTTTTCCCGTTCGTCGCTTTCGCCACATTCTTGGCGACTTTTTGGTTAACGGTG
CATCTCGGGGCAATTAAGTC

Downstream 100 bases:

>100_bases
GCGTTGTGATAGACCGACACCGAACGTAAACCGCGTGAGACGGTGGTGGCGATCACACAGGCCAACAGGATCCCCGGTAG
CAGGGTATATTCACCGGTCA

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase 2; DTBS 2; Dethiobiotin synthase 2 [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MLTRLFVTGTDTDVGKTVVSRALLQALAAQGRSVAGYKPVAARCQETSEGLRNKDALVLQASSTMPLSYQEINPITCLEE
VFHAQPTKETNYGVMSSGLQHLSTKADTVVVEGSGGWRVLMNDMRPYAEWVIQEQLPVVLVVGIKLGCVSHALLTAQAII
NDGLPLLGWVANRINPGLAHYAETIDAMQQRIPAPLLGEIPYLPRAEQRELARYLDISPLLNR

Sequences:

>Translated_223_residues
MLTRLFVTGTDTDVGKTVVSRALLQALAAQGRSVAGYKPVAARCQETSEGLRNKDALVLQASSTMPLSYQEINPITCLEE
VFHAQPTKETNYGVMSSGLQHLSTKADTVVVEGSGGWRVLMNDMRPYAEWVIQEQLPVVLVVGIKLGCVSHALLTAQAII
NDGLPLLGWVANRINPGLAHYAETIDAMQQRIPAPLLGEIPYLPRAEQRELARYLDISPLLNR
>Mature_223_residues
MLTRLFVTGTDTDVGKTVVSRALLQALAAQGRSVAGYKPVAARCQETSEGLRNKDALVLQASSTMPLSYQEINPITCLEE
VFHAQPTKETNYGVMSSGLQHLSTKADTVVVEGSGGWRVLMNDMRPYAEWVIQEQLPVVLVVGIKLGCVSHALLTAQAII
NDGLPLLGWVANRINPGLAHYAETIDAMQQRIPAPLLGEIPYLPRAEQRELARYLDISPLLNR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=223, Percent_Identity=67.2645739910314, Blast_Score=312, Evalue=1e-86,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=50.6666666666667, Blast_Score=216, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 24344; Mature: 24344

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLFVTGTDTDVGKTVVSRALLQALAAQGRSVAGYKPVAARCQETSEGLRNKDALVLQ
CCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
ASSTMPLSYQEINPITCLEEVFHAQPTKETNYGVMSSGLQHLSTKADTVVVEGSGGWRVL
ECCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEH
MNDMRPYAEWVIQEQLPVVLVVGIKLGCVSHALLTAQAIINDGLPLLGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
YAETIDAMQQRIPAPLLGEIPYLPRAEQRELARYLDISPLLNR
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MLTRLFVTGTDTDVGKTVVSRALLQALAAQGRSVAGYKPVAARCQETSEGLRNKDALVLQ
CCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
ASSTMPLSYQEINPITCLEEVFHAQPTKETNYGVMSSGLQHLSTKADTVVVEGSGGWRVL
ECCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEH
MNDMRPYAEWVIQEQLPVVLVVGIKLGCVSHALLTAQAIINDGLPLLGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
YAETIDAMQQRIPAPLLGEIPYLPRAEQRELARYLDISPLLNR
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]