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The map label for this gene is glyS

Identifier: 15645587

GI number: 15645587

Start: 1032528

End: 1034633

Strand: Reverse

Name: glyS

Synonym: HP0972

Alternate gene names: 15645587

Gene position: 1034633-1032528 (Counterclockwise)

Preceding gene: 15645591

Following gene: 15645586

Centisome position: 62.03

GC content: 37.32

Gene sequence:

>2106_bases
TTGCATTCAGATGAATTGTTAGTAGAGATTTTAGTTGAAGAATTGCCCGCGCAAGCGTTATTGAATGAATATAAAGAAAT
GCCTAAAAAACTCCACGCTCTTTTTAATAAGCGCGCTTTAGAAGTGGGAAATATAGAGATTTTTTACACCCCTAGGCGTT
TGTGTTTGCTCATTAAAGACTTTCCCCTTTTAACCCAAGAAACCAAAGAGGAATTTTTTGGGCCTCCCGTTAAAATCGCA
TGCAATCATCAAGATAAAACGCAAGGGTTGAACGCGTTAGGTTTAGGGTTTTATCAAAAATTAGGGTTAAAAGATCACCA
GTATTTCCAAACAGCGTTTAAAAATAATAAAGAAGTGCTTTATCACGCTAAAATCCATGAAAAAGAGCCTACAAAAGACT
TAATCATGCCCATTGTGTTAGAGTTTTTAGAGGGTTTGAATTTCGGAAAGTCTATGCGTTGGGGCAATGTGGAAAAAAGC
TTTATCAGACCCATTCATAATATTTGCGTGTTGTTTAATGGGGAAGATTTTAGCGGTATTGAAGTCAAAGAGTATGGCTT
TAAAACCAAGCAAGCCACAAAAGTGCACCGACAAGAGGGTTTTGATTTTATCCAGGTGGATAGCCCTAAAGCGTATTTTG
AAGTTTTAGAAAAAAACCATGTCATTTTAGACCCTAAAAAGCGCGAAGCTAAAATCTTACAAGAAATTAAAGAGCTAGAA
ACAAAGCACCACATCATTGTAGAAACAGATAGGGATTTATTAGATGAGGTGGTAGCGATCACGGAATACCCCAGCGCGCT
TTTAGGGGAGTTTGACAAGGCGTTTTTAAAATTACCCAGTGAAATCATCATCACTTCCATGAAAGAAAACCAACGCTATT
TTGCAACCTTTTGTCAAAAAAGCCAAGAAGAAAGCCCAACATTACACAACGGCTTTATTGTGGTGAGTAACGCTATCAAT
AAAGACAAGCAAAAAATCATTTTAGGCAACCAAAAGGTTTTAAAAGCCCGTTTGAGCGATGCGGTTTTCTTTTATGAAAA
CGATCTCAAAAAGCCTTTAGATAACGCCCCTTTAGAGAGTGTGGTTTTTGTGCAAGGTTTAGGGACTTTAAAAGATAAAA
TGGAGCGCGAATCAATCATCGCCCAATACTTGACGCAAAAATATATTTCATCTTTAAACATGCCTTTAGAAAAAGCCCTT
GAGTTGGTTAAAAGAGCCGTTCAAATCGCTAAGGCGGATTTACTCAGTGAAGTGGTGTATGAATTTAGCGAGCTTCAAGG
GATCATGGGCTATTACTACGCTTTAAAACAAAATGAAAACGAGTTAGTCGCCTTGAGTGTGAAAGAGCAGTATTTGCCCG
CAAGCGAAAACGCTCCCTTGCCCTCTAGCGTTTTTAGTTCAATCGTGGCTTTGAGCTTGAAATTAGACAGCCTGTTTTCT
CTTTTTAGCGTGGGTAAAATCCCTAGCGGATCTAAAGATCCTTTTGCTTTAAGGCGCTTGAGTTTTGGGCTATTGAAAAT
CATCGCGCATTACGGGTTAGAATTTGATTTGAAAGCGGATTTGAAAAACCTTTTTCAAAAAGTGGGCGTTTATCAAAGCT
TTGATTTAGAGATTTTAGAAAAGTTTTTACTGGAGCGCTTTCATAATTTAATAGATTGTAACCCCTCTATTATAAGGAGC
GTGTTAAACACCAACGAGCGAGACATTGTTAAAATCATTCAAAAAGTCAAAGCCTTAAAACGCTTTTTAGACGATCCCAA
GAACGCTCAAAAAAAAGAGTTGCTTTTTAGCGCTTTCAAACGCCTAGCCAATATCAATAAAGACAGAAACCCTAACGAGT
CAAGCGAGTTTTCAACAAATCTTTTTAAAGAGCCAAAAGAGCATGCCCTTTTTGAAGCGTTTAATGCGATCAAAATGAAC
GCTTTTGAGAGTTTGGATAGCAAAATAGAGGCTTATTTTGGTTTGCATGCACCTTTAGAAGAGTATTTTAAAAGCGTGCT
AGTCATGGATAAAGATATAGAAATCCAAAAAAATCGTAAAAATTTCTTGTGGGGCGTGTATCAAAGTTTCTTAGAAATTG
GGGATATTAAAGAAATTGCGATTTAG

Upstream 100 bases:

>100_bases
TTTGTATTAAGAAATAAACTAAACTGAGTGTAAATTCAAGTATAATTCTAACATGATTTAGTCTTAAAAGTGATTTTGTT
TTTTAGGGAGAATTGAGATT

Downstream 100 bases:

>100_bases
TGGCTTCAAACGCAAAGTCTGGCGTTTTTGTTTGGGGGTAACTTTTTTAATGAGCATGTTTAGTGCGGGCATGCTTAGTG
CTAAAATCTTTACCCTACAA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGNIEIFYTPRRLCLLIKDFPLLTQETKEEFFGPPVKIA
CNHQDKTQGLNALGLGFYQKLGLKDHQYFQTAFKNNKEVLYHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKS
FIRPIHNICVLFNGEDFSGIEVKEYGFKTKQATKVHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
TKHHIIVETDRDLLDEVVAITEYPSALLGEFDKAFLKLPSEIIITSMKENQRYFATFCQKSQEESPTLHNGFIVVSNAIN
KDKQKIILGNQKVLKARLSDAVFFYENDLKKPLDNAPLESVVFVQGLGTLKDKMERESIIAQYLTQKYISSLNMPLEKAL
ELVKRAVQIAKADLLSEVVYEFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSSIVALSLKLDSLFS
LFSVGKIPSGSKDPFALRRLSFGLLKIIAHYGLEFDLKADLKNLFQKVGVYQSFDLEILEKFLLERFHNLIDCNPSIIRS
VLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKRLANINKDRNPNESSEFSTNLFKEPKEHALFEAFNAIKMN
AFESLDSKIEAYFGLHAPLEEYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI

Sequences:

>Translated_701_residues
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGNIEIFYTPRRLCLLIKDFPLLTQETKEEFFGPPVKIA
CNHQDKTQGLNALGLGFYQKLGLKDHQYFQTAFKNNKEVLYHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKS
FIRPIHNICVLFNGEDFSGIEVKEYGFKTKQATKVHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
TKHHIIVETDRDLLDEVVAITEYPSALLGEFDKAFLKLPSEIIITSMKENQRYFATFCQKSQEESPTLHNGFIVVSNAIN
KDKQKIILGNQKVLKARLSDAVFFYENDLKKPLDNAPLESVVFVQGLGTLKDKMERESIIAQYLTQKYISSLNMPLEKAL
ELVKRAVQIAKADLLSEVVYEFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSSIVALSLKLDSLFS
LFSVGKIPSGSKDPFALRRLSFGLLKIIAHYGLEFDLKADLKNLFQKVGVYQSFDLEILEKFLLERFHNLIDCNPSIIRS
VLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKRLANINKDRNPNESSEFSTNLFKEPKEHALFEAFNAIKMN
AFESLDSKIEAYFGLHAPLEEYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI
>Mature_701_residues
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGNIEIFYTPRRLCLLIKDFPLLTQETKEEFFGPPVKIA
CNHQDKTQGLNALGLGFYQKLGLKDHQYFQTAFKNNKEVLYHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKS
FIRPIHNICVLFNGEDFSGIEVKEYGFKTKQATKVHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
TKHHIIVETDRDLLDEVVAITEYPSALLGEFDKAFLKLPSEIIITSMKENQRYFATFCQKSQEESPTLHNGFIVVSNAIN
KDKQKIILGNQKVLKARLSDAVFFYENDLKKPLDNAPLESVVFVQGLGTLKDKMERESIIAQYLTQKYISSLNMPLEKAL
ELVKRAVQIAKADLLSEVVYEFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSSIVALSLKLDSLFS
LFSVGKIPSGSKDPFALRRLSFGLLKIIAHYGLEFDLKADLKNLFQKVGVYQSFDLEILEKFLLERFHNLIDCNPSIIRS
VLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKRLANINKDRNPNESSEFSTNLFKEPKEHALFEAFNAIKMN
AFESLDSKIEAYFGLHAPLEEYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=717, Percent_Identity=29.9860529986053, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80636; Mature: 80636

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGNIEIFYTPRRLCLLIKD
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEEECCHHHHHHHHC
FPLLTQETKEEFFGPPVKIACNHQDKTQGLNALGLGFYQKLGLKDHQYFQTAFKNNKEVL
CCHHHHHHHHHHCCCCEEEEECCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
YHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKSFIRPIHNICVLFNGEDFSGI
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCC
EVKEYGFKTKQATKVHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
CHHHHCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
TKHHIIVETDRDLLDEVVAITEYPSALLGEFDKAFLKLPSEIIITSMKENQRYFATFCQK
HCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHH
SQEESPTLHNGFIVVSNAINKDKQKIILGNQKVLKARLSDAVFFYENDLKKPLDNAPLES
CCCCCCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHEEEHHHHCCCCCCCCHHH
VVFVQGLGTLKDKMERESIIAQYLTQKYISSLNMPLEKALELVKRAVQIAKADLLSEVVY
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSSIVALSLKLDSLFS
HHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LFSVGKIPSGSKDPFALRRLSFGLLKIIAHYGLEFDLKADLKNLFQKVGVYQSFDLEILE
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCHHHHH
KFLLERFHNLIDCNPSIIRSVLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFK
HHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RLANINKDRNPNESSEFSTNLFKEPKEHALFEAFNAIKMNAFESLDSKIEAYFGLHAPLE
HHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGNIEIFYTPRRLCLLIKD
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEEECCHHHHHHHHC
FPLLTQETKEEFFGPPVKIACNHQDKTQGLNALGLGFYQKLGLKDHQYFQTAFKNNKEVL
CCHHHHHHHHHHCCCCEEEEECCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
YHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKSFIRPIHNICVLFNGEDFSGI
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCC
EVKEYGFKTKQATKVHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
CHHHHCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
TKHHIIVETDRDLLDEVVAITEYPSALLGEFDKAFLKLPSEIIITSMKENQRYFATFCQK
HCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHH
SQEESPTLHNGFIVVSNAINKDKQKIILGNQKVLKARLSDAVFFYENDLKKPLDNAPLES
CCCCCCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHEEEHHHHCCCCCCCCHHH
VVFVQGLGTLKDKMERESIIAQYLTQKYISSLNMPLEKALELVKRAVQIAKADLLSEVVY
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSSIVALSLKLDSLFS
HHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LFSVGKIPSGSKDPFALRRLSFGLLKIIAHYGLEFDLKADLKNLFQKVGVYQSFDLEILE
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCHHHHH
KFLLERFHNLIDCNPSIIRSVLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFK
HHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RLANINKDRNPNESSEFSTNLFKEPKEHALFEAFNAIKMNAFESLDSKIEAYFGLHAPLE
HHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA