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The map label for this gene is cht60 [H]

Identifier: 15594965

GI number: 15594965

Start: 646659

End: 648326

Strand: Reverse

Name: cht60 [H]

Synonym: BB0620

Alternate gene names: 15594965

Gene position: 648326-646659 (Counterclockwise)

Preceding gene: 15594967

Following gene: 15594963

Centisome position: 71.19

GC content: 26.26

Gene sequence:

>1668_bases
ATGAAAAGAAATTTTTATCTCATTGTCCTTTTTATAGCTAATAACTGCTTTTCTATTGATTTTTGGGATACGATGGAAAG
AGAAAAATTAATAAATGAAATGGTAAGCAAAATGCAAGATCATGAATTACTGGGGCAAATGTTCATGATAAGCTACCCAA
ATCAATCAATCACAAATTTTGTTCTTGATTTTATAAGTAAAAAAAATCTTGGGGGAATTAAAATTTTTGGATGGAACGCA
AAAGATTTAAAAAATTTAACAGAAAGTATTCATAAAGCTCAAAAAACATCTCAAAATAATAAATTTAAAATTCCTTTATT
TGTAGCAACAGATCAAGAAGGGGGATTGGCGCAGCACATAAAATTAAATACATCAGAAACAATTGGCAATCTTGGAATTG
CAGCATCGCTTTCTCCAAAAGATTCTTATAATACAGGATATTACATAGCACAAGAGCTAAGGCAACTTGGAATAAATCTA
AACTTTGCGCCCATAGTAGATATATACAGCCATGAAAATAATTTTGCAATAGGACCAAGAACATATTCGGATAACCCCAA
AATAGTATCACTTCTTTCTCTGGCCTTTTATAAAGGACAAAAGCAAGGAGGAGTAATTTCTACTGCAAAACATTTCCCAG
GACACGGCAATACCACTCTTGACTCTCATATCAATATTCCAATAATAAATTCTAATTTACTAGAAATAAGCTTAAATGAA
CTTTTACCATATAAAATATTAATCCAAGAAAACATCCCCGTAATAATGACAGGTCATTTAGCATATCCAAAGCTTACAAA
TGGAGAAAATATTCCTGCATCATCCTCAATAAAAATAATTAAAGACATACTTAGAAAAAAATTAAAATATAATAACATAA
TAATTACTGATGACCTATTAATGAACGCAGTAAACTACAATAATGAGAGCATTTATAATACGATTGAAAGAATAGTTAGA
ACCAAAAGTGACATTTTTTTAATATCTTTAAATGAAAATATACAACAAAATGCTTACAACATGCTATTAAATTTAATGAA
AAAAGATTCAGAAATAAAAAACAATATTATTGAATCTAATAAAAGAATATTAAGAATAAAATTAATGTACTTAAAAGAAA
ATAAAAATAAATCTGATCTTTATCCTAATTTAAACAAAAATGAGAAAATATATTCAAAAGAAGGTGAAAAATTTTTTGAA
CAAAACACATTAAGGAGTATTACAAAAGTAAGAATAGAAAAAGAAATATCTAAAACCAAAAAAACACTTATAATATCTCC
TTACTACAAAATGATTGTAGAGGGTAAAAAAATATTTCAAAATACATACGCTTATTATTACAACTATTATCCCTTAAACG
GAATTAATCCCCAAAAACTCGACGAAATTAAAAAATTAATTAATAAATTTGAACAAGTAATATTTAATTTATCCACACCT
GGAAGCTTGAAATATTTAGAAAATTTGAAAGAATACAAAGACAAAATAAGCGTAATTGTATCTCTTACTCCTCACCATAT
TAAAAAATTAAATTGGATAAAAAACATAGTAATTATTTATGGAACAACACCCTTGGCATTTAAATCTGGATTTTTAACAC
TCACTAAAGATTTTGATCCAAAAGGAACCATCCCTTTAAAAAATATTATAAATAAATATTATCCTTAA

Upstream 100 bases:

>100_bases
TCAATGTTATTATTCAAAAGTATTATTATAAAGTATAATACAAAATGGCTTGCTAATAAATATAAATTACTAAATTAGCA
TTTATTATTTATATGGTTAA

Downstream 100 bases:

>100_bases
TGTATGCTAGCATTCGATTTTTTACAATCTCTAGAGAACCTTGTTTGATTCTAAACCCACTGGCATTTTTATGCCCACCG
CCTCCAAAATCTTCTGCCAA

Product: beta-glucosidase

Products: NA

Alternate protein names: Beta-N-acetylhexosaminidase; Chitobiase; N-acetyl-beta-glucosaminidase [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKRNFYLIVLFIANNCFSIDFWDTMEREKLINEMVSKMQDHELLGQMFMISYPNQSITNFVLDFISKKNLGGIKIFGWNA
KDLKNLTESIHKAQKTSQNNKFKIPLFVATDQEGGLAQHIKLNTSETIGNLGIAASLSPKDSYNTGYYIAQELRQLGINL
NFAPIVDIYSHENNFAIGPRTYSDNPKIVSLLSLAFYKGQKQGGVISTAKHFPGHGNTTLDSHINIPIINSNLLEISLNE
LLPYKILIQENIPVIMTGHLAYPKLTNGENIPASSSIKIIKDILRKKLKYNNIIITDDLLMNAVNYNNESIYNTIERIVR
TKSDIFLISLNENIQQNAYNMLLNLMKKDSEIKNNIIESNKRILRIKLMYLKENKNKSDLYPNLNKNEKIYSKEGEKFFE
QNTLRSITKVRIEKEISKTKKTLIISPYYKMIVEGKKIFQNTYAYYYNYYPLNGINPQKLDEIKKLINKFEQVIFNLSTP
GSLKYLENLKEYKDKISVIVSLTPHHIKKLNWIKNIVIIYGTTPLAFKSGFLTLTKDFDPKGTIPLKNIINKYYP

Sequences:

>Translated_555_residues
MKRNFYLIVLFIANNCFSIDFWDTMEREKLINEMVSKMQDHELLGQMFMISYPNQSITNFVLDFISKKNLGGIKIFGWNA
KDLKNLTESIHKAQKTSQNNKFKIPLFVATDQEGGLAQHIKLNTSETIGNLGIAASLSPKDSYNTGYYIAQELRQLGINL
NFAPIVDIYSHENNFAIGPRTYSDNPKIVSLLSLAFYKGQKQGGVISTAKHFPGHGNTTLDSHINIPIINSNLLEISLNE
LLPYKILIQENIPVIMTGHLAYPKLTNGENIPASSSIKIIKDILRKKLKYNNIIITDDLLMNAVNYNNESIYNTIERIVR
TKSDIFLISLNENIQQNAYNMLLNLMKKDSEIKNNIIESNKRILRIKLMYLKENKNKSDLYPNLNKNEKIYSKEGEKFFE
QNTLRSITKVRIEKEISKTKKTLIISPYYKMIVEGKKIFQNTYAYYYNYYPLNGINPQKLDEIKKLINKFEQVIFNLSTP
GSLKYLENLKEYKDKISVIVSLTPHHIKKLNWIKNIVIIYGTTPLAFKSGFLTLTKDFDPKGTIPLKNIINKYYP
>Mature_555_residues
MKRNFYLIVLFIANNCFSIDFWDTMEREKLINEMVSKMQDHELLGQMFMISYPNQSITNFVLDFISKKNLGGIKIFGWNA
KDLKNLTESIHKAQKTSQNNKFKIPLFVATDQEGGLAQHIKLNTSETIGNLGIAASLSPKDSYNTGYYIAQELRQLGINL
NFAPIVDIYSHENNFAIGPRTYSDNPKIVSLLSLAFYKGQKQGGVISTAKHFPGHGNTTLDSHINIPIINSNLLEISLNE
LLPYKILIQENIPVIMTGHLAYPKLTNGENIPASSSIKIIKDILRKKLKYNNIIITDDLLMNAVNYNNESIYNTIERIVR
TKSDIFLISLNENIQQNAYNMLLNLMKKDSEIKNNIIESNKRILRIKLMYLKENKNKSDLYPNLNKNEKIYSKEGEKFFE
QNTLRSITKVRIEKEISKTKKTLIISPYYKMIVEGKKIFQNTYAYYYNYYPLNGINPQKLDEIKKLINKFEQVIFNLSTP
GSLKYLENLKEYKDKISVIVSLTPHHIKKLNWIKNIVIIYGTTPLAFKSGFLTLTKDFDPKGTIPLKNIINKYYP

Specific function: Most active towards p-nitrophenyl-N-acetyl-beta-D- glucosaminide(PNP-beta-GlcNAc) and diacetylchitobiose [H]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=244, Percent_Identity=31.1475409836066, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3 [H]

EC number: =3.2.1.52 [H]

Molecular weight: Translated: 63965; Mature: 63965

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNFYLIVLFIANNCFSIDFWDTMEREKLINEMVSKMQDHELLGQMFMISYPNQSITNF
CCCCEEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHH
VLDFISKKNLGGIKIFGWNAKDLKNLTESIHKAQKTSQNNKFKIPLFVATDQEGGLAQHI
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEE
KLNTSETIGNLGIAASLSPKDSYNTGYYIAQELRQLGINLNFAPIVDIYSHENNFAIGPR
EECCHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCEEECCC
TYSDNPKIVSLLSLAFYKGQKQGGVISTAKHFPGHGNTTLDSHINIPIINSNLLEISLNE
CCCCCCHHHHHHHHHHHCCCCCCCEEEEHHCCCCCCCCEECCCEEEEEECCCEEEEEHHH
LLPYKILIQENIPVIMTGHLAYPKLTNGENIPASSSIKIIKDILRKKLKYNNIIITDDLL
CCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHH
MNAVNYNNESIYNTIERIVRTKSDIFLISLNENIQQNAYNMLLNLMKKDSEIKNNIIESN
HHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCC
KRILRIKLMYLKENKNKSDLYPNLNKNEKIYSKEGEKFFEQNTLRSITKVRIEKEISKTK
CEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLIISPYYKMIVEGKKIFQNTYAYYYNYYPLNGINPQKLDEIKKLINKFEQVIFNLSTP
HEEEECHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCC
GSLKYLENLKEYKDKISVIVSLTPHHIKKLNWIKNIVIIYGTTPLAFKSGFLTLTKDFDP
CHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHCCEEEEECCCCHHHCCCCEEEEECCCC
KGTIPLKNIINKYYP
CCCCCHHHHHHHCCC
>Mature Secondary Structure
MKRNFYLIVLFIANNCFSIDFWDTMEREKLINEMVSKMQDHELLGQMFMISYPNQSITNF
CCCCEEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHH
VLDFISKKNLGGIKIFGWNAKDLKNLTESIHKAQKTSQNNKFKIPLFVATDQEGGLAQHI
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEE
KLNTSETIGNLGIAASLSPKDSYNTGYYIAQELRQLGINLNFAPIVDIYSHENNFAIGPR
EECCHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCEEECCC
TYSDNPKIVSLLSLAFYKGQKQGGVISTAKHFPGHGNTTLDSHINIPIINSNLLEISLNE
CCCCCCHHHHHHHHHHHCCCCCCCEEEEHHCCCCCCCCEECCCEEEEEECCCEEEEEHHH
LLPYKILIQENIPVIMTGHLAYPKLTNGENIPASSSIKIIKDILRKKLKYNNIIITDDLL
CCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHH
MNAVNYNNESIYNTIERIVRTKSDIFLISLNENIQQNAYNMLLNLMKKDSEIKNNIIESN
HHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCC
KRILRIKLMYLKENKNKSDLYPNLNKNEKIYSKEGEKFFEQNTLRSITKVRIEKEISKTK
CEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLIISPYYKMIVEGKKIFQNTYAYYYNYYPLNGINPQKLDEIKKLINKFEQVIFNLSTP
HEEEECHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCC
GSLKYLENLKEYKDKISVIVSLTPHHIKKLNWIKNIVIIYGTTPLAFKSGFLTLTKDFDP
CHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHCCEEEEECCCCHHHCCCCEEEEECCCC
KGTIPLKNIINKYYP
CCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063094 [H]