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The map label for this gene is ackA [H]

Identifier: 15594967

GI number: 15594967

Start: 649170

End: 650411

Strand: Reverse

Name: ackA [H]

Synonym: BB0622

Alternate gene names: 15594967

Gene position: 650411-649170 (Counterclockwise)

Preceding gene: 15594968

Following gene: 15594965

Centisome position: 71.42

GC content: 28.99

Gene sequence:

>1242_bases
TTGTCAAATACAAAAGGAAAAAAAATGAAAATATTAATCATAAACACAGGAAGTTCTTCATTAAAATTTGCTATTTATCA
ATATGAAAATTCAAAAAAAATAATATCTGGAATTGTTGAAAAAATAAAATCACAAAAATCAATCATAAAAATTGTAAATA
CTGACGGATCAACAACAGAAAGATTTGAAAAAGGAATTGAAAATCACCAAAAAGCAATAGAAAAAATGTTTAAAATACTC
ACAAACAGCGATTTAAAAATCCTTAAAACTCTTAGCGAAATTAAAATAATAGGACACAGGGTTGTACATGGAGGATCAAG
CCTTAAAAATTCAGTAATTCTTAATAATAGTATTTTAAATAAATTAAAACAAATTTCTGAACTTGCTCCACTTCATAACC
CAAATGCAATCACCGCAATAGAAGCGGTGCTTAAAATTTTACCACACGCAAAGCAAGTTTTATGCTTCGATACATCCTGG
CATCAAACTATAAAAGAACATGCCTTTCTTTATGCAATTCCATATTCTTGGTATAAAAACCACAATATTAGAAAATATGG
CTTTCATGGCCTTTCTTATTCGTACATAACAAAAAGATCCTCAGAAATTTTAAATAAAAAAATAGATAGTCTAAATTTAA
TAATATTGCATCTTGGTAACGGAGCAAGTATTAATGCTGTTAAAAATGGAAAATCTTATGACACAAGCATGGGAATTACT
CCGCTTGAAGGCCTTGCAATGGGAACAAGAAGCGGTGATATAGACCCATCAATTATTAATTTGATGAGCACTATATTAAA
CAAAACCACCAAACAAATTGAAGAAATATTAAATAAAGAAAGTGGCATACTGGGAATTTCTGAAAAATCAAATGACATGC
GGGATATTTGGAACAAAATTGAAGAAGGAGAATATCAATCAAAACTTGCAGTAGAAATAATGACATATAGAATAAAAAAA
TATATTGGATCTTACATTGCCGCTCTTGATTTTAATGTCGATGCAATAGTTTTTACAGGCGGAATTGGTGTTACTGATTA
TGGAATAAGAGCTCTTGCACTAAAGGGGTTTGAAAAAATTGGAATAGAACTGGACCTTGAAAAAAATGAAATGGCCCAAA
GTAAGTATTTAGAATCTGAAATATCAACCATTAATAGCAAACTAAAAATACTGGCAATACCAACAAACGAAGAATCAACC
ATTCTTGAAGACATTTATAATTTAATTCCAAAAAATTTATAA

Upstream 100 bases:

>100_bases
TATCAAAAATCAATATTTTTAAGCTTTAAAAATATTGAAAAATCTGAAAAAATAAATTACATATATAAAAATATTAACTT
ACTAAAAACAAGCACATAAC

Downstream 100 bases:

>100_bases
TTTACAATTTTAAAAACTAAAATCTGGTTTATTTATTATAATCATTTAAAATAGATTTATATTTAACTCTTGCAAAGCAA
AGTTGCTTTTTTAACATCTT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSNTKGKKMKILIINTGSSSLKFAIYQYENSKKIISGIVEKIKSQKSIIKIVNTDGSTTERFEKGIENHQKAIEKMFKIL
TNSDLKILKTLSEIKIIGHRVVHGGSSLKNSVILNNSILNKLKQISELAPLHNPNAITAIEAVLKILPHAKQVLCFDTSW
HQTIKEHAFLYAIPYSWYKNHNIRKYGFHGLSYSYITKRSSEILNKKIDSLNLIILHLGNGASINAVKNGKSYDTSMGIT
PLEGLAMGTRSGDIDPSIINLMSTILNKTTKQIEEILNKESGILGISEKSNDMRDIWNKIEEGEYQSKLAVEIMTYRIKK
YIGSYIAALDFNVDAIVFTGGIGVTDYGIRALALKGFEKIGIELDLEKNEMAQSKYLESEISTINSKLKILAIPTNEEST
ILEDIYNLIPKNL

Sequences:

>Translated_413_residues
MSNTKGKKMKILIINTGSSSLKFAIYQYENSKKIISGIVEKIKSQKSIIKIVNTDGSTTERFEKGIENHQKAIEKMFKIL
TNSDLKILKTLSEIKIIGHRVVHGGSSLKNSVILNNSILNKLKQISELAPLHNPNAITAIEAVLKILPHAKQVLCFDTSW
HQTIKEHAFLYAIPYSWYKNHNIRKYGFHGLSYSYITKRSSEILNKKIDSLNLIILHLGNGASINAVKNGKSYDTSMGIT
PLEGLAMGTRSGDIDPSIINLMSTILNKTTKQIEEILNKESGILGISEKSNDMRDIWNKIEEGEYQSKLAVEIMTYRIKK
YIGSYIAALDFNVDAIVFTGGIGVTDYGIRALALKGFEKIGIELDLEKNEMAQSKYLESEISTINSKLKILAIPTNEEST
ILEDIYNLIPKNL
>Mature_412_residues
SNTKGKKMKILIINTGSSSLKFAIYQYENSKKIISGIVEKIKSQKSIIKIVNTDGSTTERFEKGIENHQKAIEKMFKILT
NSDLKILKTLSEIKIIGHRVVHGGSSLKNSVILNNSILNKLKQISELAPLHNPNAITAIEAVLKILPHAKQVLCFDTSWH
QTIKEHAFLYAIPYSWYKNHNIRKYGFHGLSYSYITKRSSEILNKKIDSLNLIILHLGNGASINAVKNGKSYDTSMGITP
LEGLAMGTRSGDIDPSIINLMSTILNKTTKQIEEILNKESGILGISEKSNDMRDIWNKIEEGEYQSKLAVEIMTYRIKKY
IGSYIAALDFNVDAIVFTGGIGVTDYGIRALALKGFEKIGIELDLEKNEMAQSKYLESEISTINSKLKILAIPTNEESTI
LEDIYNLIPKNL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=401, Percent_Identity=41.1471321695761, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI145693184, Length=398, Percent_Identity=37.9396984924623, Blast_Score=295, Evalue=3e-81,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 46211; Mature: 46080

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTKGKKMKILIINTGSSSLKFAIYQYENSKKIISGIVEKIKSQKSIIKIVNTDGSTTE
CCCCCCCEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
RFEKGIENHQKAIEKMFKILTNSDLKILKTLSEIKIIGHRVVHGGSSLKNSVILNNSILN
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHH
KLKQISELAPLHNPNAITAIEAVLKILPHAKQVLCFDTSWHQTIKEHAFLYAIPYSWYKN
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCEEEEEECHHHHCC
HNIRKYGFHGLSYSYITKRSSEILNKKIDSLNLIILHLGNGASINAVKNGKSYDTSMGIT
CCCEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCCC
PLEGLAMGTRSGDIDPSIINLMSTILNKTTKQIEEILNKESGILGISEKSNDMRDIWNKI
CCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHH
EEGEYQSKLAVEIMTYRIKKYIGSYIAALDFNVDAIVFTGGIGVTDYGIRALALKGFEKI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHHHC
GIELDLEKNEMAQSKYLESEISTINSKLKILAIPTNEESTILEDIYNLIPKNL
CEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNTKGKKMKILIINTGSSSLKFAIYQYENSKKIISGIVEKIKSQKSIIKIVNTDGSTTE
CCCCCCEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
RFEKGIENHQKAIEKMFKILTNSDLKILKTLSEIKIIGHRVVHGGSSLKNSVILNNSILN
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHH
KLKQISELAPLHNPNAITAIEAVLKILPHAKQVLCFDTSWHQTIKEHAFLYAIPYSWYKN
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCEEEEEECHHHHCC
HNIRKYGFHGLSYSYITKRSSEILNKKIDSLNLIILHLGNGASINAVKNGKSYDTSMGIT
CCCEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCCC
PLEGLAMGTRSGDIDPSIINLMSTILNKTTKQIEEILNKESGILGISEKSNDMRDIWNKI
CCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHH
EEGEYQSKLAVEIMTYRIKKYIGSYIAALDFNVDAIVFTGGIGVTDYGIRALALKGFEKI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHHHC
GIELDLEKNEMAQSKYLESEISTINSKLKILAIPTNEESTILEDIYNLIPKNL
CEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]