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The map label for this gene is mtlK [H]

Identifier: 150397553

GI number: 150397553

Start: 2428481

End: 2429965

Strand: Reverse

Name: mtlK [H]

Synonym: Smed_2353

Alternate gene names: 150397553

Gene position: 2429965-2428481 (Counterclockwise)

Preceding gene: 150397554

Following gene: 150397552

Centisome position: 64.25

GC content: 60.74

Gene sequence:

>1485_bases
ATGACGACCAAACTCTCGCTTGCCACGCTCGATGCGGTCAGGGCCAAGGCTGGCATTCCGAATTATGGCCGGCACGATCT
CAGGGCCGGCATCGTTCATTTCGGCGTCGGAAACTTTCATCGCGCCCATCAGGCGGTTTATCTCGACGATCTCTTCAACA
TGGGACGCGACCGGGATTGGGCGATTATCGGCGCGGGTGTCCTTCCATCGGACAGGATCATGCGCGACAAGCTCGAGGCG
CAGGACTTCCTGACGACGGTGGTGGAGCAGGACAACAACCGGACGGGCGCACATGTCACGGGGGCCATGATCGCTTATCT
CGAGCCGGGTGACGTGCCGGCGATCGTGGCGCAACTCGCGAGCCCCTTAATCCGCATCGTTTCCTTGACCATCACCGAAG
GCGGCTATTTCATCGATCCAGCTTCCGGGGTCTTCGACCCTGCCCATCCGGCTATCGCCGAGGATGCGCGAAACCCACTG
GCGCCGAAAACCGTCTTCGGTCTTATCCTTGCCGGGCTCGCCGAACGGAGAGCCAAGGGCATTCCGCCGTTTACGATCAT
GTCCTGCGACAACATCCCCGGAAACGGCGAAGTCACCCACGCTGCCGTTTCCGGCCTTGCGCGCCTGTCGGATCCTGGCT
TCGCGGATTGGATCGACGCCAATGTCGCCTTTCCCAATGGCATGGTCGATCGCATCACGCCTGCAACCGGCACGCGCGAG
ATCGGCATCGTTGTGTCGGAATACGGGATCGACGATGCCTGGCCGGTCTTCTGCGAGGAGTTCAAGCAATGGGTGCTGGA
GGACCGCTTCCCGCAGGGTCGCCCGGCGCTCGAAGAGGTCGGCGTGCAGTTCGTCCCCGATGTGGCGCCGTACGAGCACA
TGAAGATACGCATTCTCAACGGCGGCCATGCTGCCATCGCCTATCCGGCGGCATTGCTCGACATACACTTCGTCCATGAG
GCGATGGCGGAACCGCTGATCCGCGCTTTCCTGGAGAAACTGGAGCATGAAGAGATCATCCCCGTCATTCCGCCGGTTCC
GGACACGGATCTGAGGGAGTATTACAAGCTCATCGAAACGCGTTTTTCAAACCCGAAGATCGGTGACACGGTTGCTCGGC
TGGCGCAGGACGGATCGAACCGGCAGCCGAAATTCATCCTGCCATCGACGGCGGACCGCCTGCGCCGCGGCGACGACGTC
GTCGGTCTGTCGCTGGTCTCGGCGCTGTGGTGCCGCTACCTCGCCGGAAAATCCGACAGCGGCAGGGAGATCCTGTTCAA
CGACCCAAATTCGGACCGGCTGCATGCCGCTGCGCTTTTGGCAAAGGACGACCCCATGGAATTCCTCGCGCTTTCCGATA
TTTTCGGCGATGTCGCGCAATCCGATCTTTTCCGTCGCCGATTTTCCCACGCCTTGAAAACACTTTGGGAAAAAGGTACG
CGCCAAACGCTCCAGGTCTATCTGGACGGAAAGCTCGGGGAATGA

Upstream 100 bases:

>100_bases
CTCGCCTCGTCCGAAAGCGACTACGTTGTCGCTCAGACCTACAACGTCGACGGCGGCAACTGGATGAGCTGAGACGCGGG
TTCTCGAAAGGAACAACAGG

Downstream 100 bases:

>100_bases
CGATGGCGGGGAGCACCTCGAAGCTGGTGATTTTCGATTGTGATGGCGTGTTGGTGGACAGCGAGCCGATTTCGCTTGAG
GTTCTTGTGGAAGCGCTGGC

Product: mannitol dehydrogenase domain-containing protein

Products: NA

Alternate protein names: M2DH; MDH [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTTKLSLATLDAVRAKAGIPNYGRHDLRAGIVHFGVGNFHRAHQAVYLDDLFNMGRDRDWAIIGAGVLPSDRIMRDKLEA
QDFLTTVVEQDNNRTGAHVTGAMIAYLEPGDVPAIVAQLASPLIRIVSLTITEGGYFIDPASGVFDPAHPAIAEDARNPL
APKTVFGLILAGLAERRAKGIPPFTIMSCDNIPGNGEVTHAAVSGLARLSDPGFADWIDANVAFPNGMVDRITPATGTRE
IGIVVSEYGIDDAWPVFCEEFKQWVLEDRFPQGRPALEEVGVQFVPDVAPYEHMKIRILNGGHAAIAYPAALLDIHFVHE
AMAEPLIRAFLEKLEHEEIIPVIPPVPDTDLREYYKLIETRFSNPKIGDTVARLAQDGSNRQPKFILPSTADRLRRGDDV
VGLSLVSALWCRYLAGKSDSGREILFNDPNSDRLHAAALLAKDDPMEFLALSDIFGDVAQSDLFRRRFSHALKTLWEKGT
RQTLQVYLDGKLGE

Sequences:

>Translated_494_residues
MTTKLSLATLDAVRAKAGIPNYGRHDLRAGIVHFGVGNFHRAHQAVYLDDLFNMGRDRDWAIIGAGVLPSDRIMRDKLEA
QDFLTTVVEQDNNRTGAHVTGAMIAYLEPGDVPAIVAQLASPLIRIVSLTITEGGYFIDPASGVFDPAHPAIAEDARNPL
APKTVFGLILAGLAERRAKGIPPFTIMSCDNIPGNGEVTHAAVSGLARLSDPGFADWIDANVAFPNGMVDRITPATGTRE
IGIVVSEYGIDDAWPVFCEEFKQWVLEDRFPQGRPALEEVGVQFVPDVAPYEHMKIRILNGGHAAIAYPAALLDIHFVHE
AMAEPLIRAFLEKLEHEEIIPVIPPVPDTDLREYYKLIETRFSNPKIGDTVARLAQDGSNRQPKFILPSTADRLRRGDDV
VGLSLVSALWCRYLAGKSDSGREILFNDPNSDRLHAAALLAKDDPMEFLALSDIFGDVAQSDLFRRRFSHALKTLWEKGT
RQTLQVYLDGKLGE
>Mature_493_residues
TTKLSLATLDAVRAKAGIPNYGRHDLRAGIVHFGVGNFHRAHQAVYLDDLFNMGRDRDWAIIGAGVLPSDRIMRDKLEAQ
DFLTTVVEQDNNRTGAHVTGAMIAYLEPGDVPAIVAQLASPLIRIVSLTITEGGYFIDPASGVFDPAHPAIAEDARNPLA
PKTVFGLILAGLAERRAKGIPPFTIMSCDNIPGNGEVTHAAVSGLARLSDPGFADWIDANVAFPNGMVDRITPATGTREI
GIVVSEYGIDDAWPVFCEEFKQWVLEDRFPQGRPALEEVGVQFVPDVAPYEHMKIRILNGGHAAIAYPAALLDIHFVHEA
MAEPLIRAFLEKLEHEEIIPVIPPVPDTDLREYYKLIETRFSNPKIGDTVARLAQDGSNRQPKFILPSTADRLRRGDDVV
GLSLVSALWCRYLAGKSDSGREILFNDPNSDRLHAAALLAKDDPMEFLALSDIFGDVAQSDLFRRRFSHALKTLWEKGTR
QTLQVYLDGKLGE

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=471, Percent_Identity=38.0042462845011, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1788497, Length=470, Percent_Identity=36.8085106382979, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1787823, Length=481, Percent_Identity=36.3825363825364, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI48994885, Length=378, Percent_Identity=27.2486772486773, Blast_Score=95, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324401, Length=491, Percent_Identity=40.5295315682281, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6320765, Length=491, Percent_Identity=40.5295315682281, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: =1.1.1.67 [H]

Molecular weight: Translated: 54283; Mature: 54152

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKLSLATLDAVRAKAGIPNYGRHDLRAGIVHFGVGNFHRAHQAVYLDDLFNMGRDRDW
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCE
AIIGAGVLPSDRIMRDKLEAQDFLTTVVEQDNNRTGAHVTGAMIAYLEPGDVPAIVAQLA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHHHHHH
SPLIRIVSLTITEGGYFIDPASGVFDPAHPAIAEDARNPLAPKTVFGLILAGLAERRAKG
HHHHHHHHEEEECCCEEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
IPPFTIMSCDNIPGNGEVTHAAVSGLARLSDPGFADWIDANVAFPNGMVDRITPATGTRE
CCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCEECCCCCHHCCCCCCCCCE
IGIVVSEYGIDDAWPVFCEEFKQWVLEDRFPQGRPALEEVGVQFVPDVAPYEHMKIRILN
EEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEECCCCCCCCCEEEEEEE
GGHAAIAYPAALLDIHFVHEAMAEPLIRAFLEKLEHEEIIPVIPPVPDTDLREYYKLIET
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
RFSNPKIGDTVARLAQDGSNRQPKFILPSTADRLRRGDDVVGLSLVSALWCRYLAGKSDS
HCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
GREILFNDPNSDRLHAAALLAKDDPMEFLALSDIFGDVAQSDLFRRRFSHALKTLWEKGT
CCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RQTLQVYLDGKLGE
CEEEEEEECCCCCC
>Mature Secondary Structure 
TTKLSLATLDAVRAKAGIPNYGRHDLRAGIVHFGVGNFHRAHQAVYLDDLFNMGRDRDW
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCE
AIIGAGVLPSDRIMRDKLEAQDFLTTVVEQDNNRTGAHVTGAMIAYLEPGDVPAIVAQLA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHHHHHH
SPLIRIVSLTITEGGYFIDPASGVFDPAHPAIAEDARNPLAPKTVFGLILAGLAERRAKG
HHHHHHHHEEEECCCEEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
IPPFTIMSCDNIPGNGEVTHAAVSGLARLSDPGFADWIDANVAFPNGMVDRITPATGTRE
CCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCEECCCCCHHCCCCCCCCCE
IGIVVSEYGIDDAWPVFCEEFKQWVLEDRFPQGRPALEEVGVQFVPDVAPYEHMKIRILN
EEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEECCCCCCCCCEEEEEEE
GGHAAIAYPAALLDIHFVHEAMAEPLIRAFLEKLEHEEIIPVIPPVPDTDLREYYKLIET
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
RFSNPKIGDTVARLAQDGSNRQPKFILPSTADRLRRGDDVVGLSLVSALWCRYLAGKSDS
HCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
GREILFNDPNSDRLHAAALLAKDDPMEFLALSDIFGDVAQSDLFRRRFSHALKTLWEKGT
CCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RQTLQVYLDGKLGE
CEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8254318 [H]