Click here to switch to the map view.

The map label for this gene is yieH [H]

Identifier: 150397552

GI number: 150397552

Start: 2427810

End: 2428478

Strand: Reverse

Name: yieH [H]

Synonym: Smed_2352

Alternate gene names: 150397552

Gene position: 2428478-2427810 (Counterclockwise)

Preceding gene: 150397553

Following gene: 150397551

Centisome position: 64.21

GC content: 62.48

Gene sequence:

>669_bases
ATGGCGGGGAGCACCTCGAAGCTGGTGATTTTCGATTGTGATGGCGTGTTGGTGGACAGCGAGCCGATTTCGCTTGAGGT
TCTTGTGGAAGCGCTGGCGGCCGCCGGCGTTGCGATGACGACGGAGGAGGCGAGCGAGCGCTTCCTTGGCCGCAGCCTCA
AGACCATGTCGGCCATCCTGCATGAAGAACACGGGCTTGCGACCGACGAGGCTTTTCTCGAGGGGATGCGGACCCGGCTC
TATGCGCGCTTTCGCGAGGAATTGAGGCCCGTCGCCGGCATACGCGAGGCGGTCGAACGGCTGGGGACCGCCCATTGTGT
GGCTTCGTCGAGCCAGCCTGAGCGCATTCGTCTTTCGCTGACGGTGACGGGGCTCATCGATCTTTTTGAGCCAAGTATTT
TCAGCGCCAGCATGGTGTCACGCGGCAAGCCGGCGCCCGACCTTTTCCTGCATGCAAGTGCCGAGATGGGGTACGATCCG
TCCGACTGCGTCGTCGTCGAAGACAGCCCGGCGGGCATCGAGGCCGCAAAGGCCGCGGGCATGCGGGTCTTCGCATTTGC
GGGGGCCAGCCATGCGCGAAACGAGCGTCATCGGCGGGTGCTTGCCAGTCTTGACCCCGACGTGCTGTTTGACGACATGC
ATGAATTGATACAGTTTGTGCGGCAATAG

Upstream 100 bases:

>100_bases
TTTTCCGTCGCCGATTTTCCCACGCCTTGAAAACACTTTGGGAAAAAGGTACGCGCCAAACGCTCCAGGTCTATCTGGAC
GGAAAGCTCGGGGAATGACG

Downstream 100 bases:

>100_bases
AACACTTGCGGGAAAGAATGCTTGCGGGAAAAACGTGTGACGGTTTTCCGGTCGCACCGTTTCTTCGGCGGCAATTCATC
ACCTTTTTTCGGGACGGGGG

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MAGSTSKLVIFDCDGVLVDSEPISLEVLVEALAAAGVAMTTEEASERFLGRSLKTMSAILHEEHGLATDEAFLEGMRTRL
YARFREELRPVAGIREAVERLGTAHCVASSSQPERIRLSLTVTGLIDLFEPSIFSASMVSRGKPAPDLFLHASAEMGYDP
SDCVVVEDSPAGIEAAKAAGMRVFAFAGASHARNERHRRVLASLDPDVLFDDMHELIQFVRQ

Sequences:

>Translated_222_residues
MAGSTSKLVIFDCDGVLVDSEPISLEVLVEALAAAGVAMTTEEASERFLGRSLKTMSAILHEEHGLATDEAFLEGMRTRL
YARFREELRPVAGIREAVERLGTAHCVASSSQPERIRLSLTVTGLIDLFEPSIFSASMVSRGKPAPDLFLHASAEMGYDP
SDCVVVEDSPAGIEAAKAAGMRVFAFAGASHARNERHRRVLASLDPDVLFDDMHELIQFVRQ
>Mature_221_residues
AGSTSKLVIFDCDGVLVDSEPISLEVLVEALAAAGVAMTTEEASERFLGRSLKTMSAILHEEHGLATDEAFLEGMRTRLY
ARFREELRPVAGIREAVERLGTAHCVASSSQPERIRLSLTVTGLIDLFEPSIFSASMVSRGKPAPDLFLHASAEMGYDPS
DCVVVEDSPAGIEAAKAAGMRVFAFAGASHARNERHRRVLASLDPDVLFDDMHELIQFVRQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=193, Percent_Identity=31.6062176165803, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788021, Length=217, Percent_Identity=32.7188940092166, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1789046, Length=179, Percent_Identity=31.2849162011173, Blast_Score=65, Evalue=4e-12,
Organism=Drosophila melanogaster, GI116008157, Length=210, Percent_Identity=32.8571428571429, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137324, Length=216, Percent_Identity=30.5555555555556, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20129151, Length=203, Percent_Identity=26.1083743842365, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24104; Mature: 23973

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSTSKLVIFDCDGVLVDSEPISLEVLVEALAAAGVAMTTEEASERFLGRSLKTMSAIL
CCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
HEEHGLATDEAFLEGMRTRLYARFREELRPVAGIREAVERLGTAHCVASSSQPERIRLSL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCEEEEEE
TVTGLIDLFEPSIFSASMVSRGKPAPDLFLHASAEMGYDPSDCVVVEDSPAGIEAAKAAG
EEEHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHCC
MRVFAFAGASHARNERHRRVLASLDPDVLFDDMHELIQFVRQ
EEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AGSTSKLVIFDCDGVLVDSEPISLEVLVEALAAAGVAMTTEEASERFLGRSLKTMSAIL
CCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
HEEHGLATDEAFLEGMRTRLYARFREELRPVAGIREAVERLGTAHCVASSSQPERIRLSL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCEEEEEE
TVTGLIDLFEPSIFSASMVSRGKPAPDLFLHASAEMGYDPSDCVVVEDSPAGIEAAKAAG
EEEHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHCC
MRVFAFAGASHARNERHRRVLASLDPDVLFDDMHELIQFVRQ
EEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]