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The map label for this gene is pflB [H]

Identifier: 145299308

GI number: 145299308

Start: 2502306

End: 2504594

Strand: Direct

Name: pflB [H]

Synonym: ASA_2352

Alternate gene names: 145299308

Gene position: 2502306-2504594 (Clockwise)

Preceding gene: 145299307

Following gene: 145299318

Centisome position: 53.21

GC content: 56.14

Gene sequence:

>2289_bases
ATGAAAATGGCAGAACTTAACGAACAGTTCCAAAAAGCATGGGAAGGCTTCGCGGCCGGTGAATGGCAGACCTCTGTCAA
CACCCGTGACTTCATCCAGAAGAACTACACCCCGTATGAAGGTGACGAGTCCTTCCTGGCTGGCGCGACCGAAGCTACCA
CCAAGCTGTGGGACAAGGTCATGGAAGGCATCAAGATCGAGAACCGCACCCATGCGCCGGTCGATTTTGATACCGACCTG
CCCTCCACCATTACCGCTCACGATGCCGGCTATATCGATCAGAGCCTGGAGCAAATTGTAGGTCTGCAGACCGACAAGCC
GCTCAAGCGCGCCATCATCGCCAACGGCGGTATCAAGATGGTCAAGACCTCTTGCGAAGTGTACGGTCGTCAACTTGACC
CCATGGTCGAGAAGATCTTCACCGAGTACCGCAAGACTCACAACCAGGGCGTGTTCGACGTTTACACCAAGGACATCCTG
AACTGCCGCAAGTCCGGCGTCATCACAGGTCTGCCGGATGCCTACGGTCGTGGCCGTATCATCGGTGACTACCGTCGTGT
TGCCCTGTACGGCATCGACTTCCTGATGGCCGACAAACTGGCCCAGTTCAAGTCCCTGCAAGCTGATCTGGAAAATGGCG
TCAACCTAGAAGCCACCATCCGTCTGCGCGAAGAGATCTCCGAGCAGCACCGTGCCCTGGCACAGATGAAGGTCATGGCT
GCCAAATACGGCTGCGATATCTCAGTACCGGCCAAGAACGCCAAAGAAGCCGTACAGTGGACCTACTTCGCCTACCTGGC
GGCAGTCAAGTCCCAGAACGGTGCCGCCATGTCCTTCGGTCGTACCTCCAGCTTCCTGGACGTCTACATCGAACGTGACC
TGAAAAAAGGCGTGCTCACCGAGCAAGAAGCCCAGGAGCTGATGGACCACATGGTCATGAAGCTGCGTATGGTTCGCTTC
CTGCGTACTCCCGAATACGATTCACTGTTCTCCGGCGACCCCATGTGGGCAACCGAGACCCTGGCTGGTATGGGCGTTGA
TGGCCGTACCCTGGTCACCAAGAACAGCTTCCGTATGCTGAACACCCTGTACACCATGGGCCCGTCCCCTGAGCCGAACC
TGACCATACTGTGGTCCGAGCAGCTGCCAATCGGGTTCAAGAAGTACTGTGCCAAGGTATCTATCGAGACCTCTTCCGTT
CAGTACGAGAACGACGATCTGATGCGTCCGGACTTCAACAACGATGACTATGCCATCGCTTGCTGCGTGTCCCCGATGAT
CGTCGGCAAACAGATGCAGTTCTTCGGTGCCCGTGCCAACCTGGCCAAGACCATGCTGTATGCAATCAACGGCGGTATGG
ACGAGAAACTCAAAATCCAGATCGGTCCCAAGACCGAAATGGTCAAGAGCGAGTACCTCGACTACAACGACGTGATGGAT
CGTCTGGATCACTTCATGGACTGGCTGGCCAAGCAGTACGTGGCTGCCCTGAACATCATCCACTACATGCACGACAAGTA
CAGCTACGAAGCCTCCCTGATGGCGCTGCACGACCGTGATGTCTATCGCACCATGGCTTGCGGCATCGCCGGTCTGTCTG
TTGCGGCTGACTCCCTGTCTGCCATCAAGTACGCCAAGGTCAAGCCGGTTCGTGACGAAGATGGCGTTGCCATCGACTTC
GAAATCGACGGCGAATACCCGCAGTTCGGTAACAACGACGCTCGCGTCGATGACATCGCCTGTGACCTGGTTGAACGCTT
CATGAAGAAAATTCAGAAGCTGAACACCTACCGTGGCGCCGTGGCGACCCAGTCCGTACTGACCATCACCTCCAACGTCG
TGTATGGCAAGAAGACCGGTAACACCCCGGATGGTCGTCGCTCAGGCGCGCCGTTTGGCCCGGGTGCCAACCCGATGCAC
GGTCGTGACCAGAAGGGTGCCGTTGCTTCCCTGACTTCCGTTGCCAAGCTGCCGTTCGCTTACGCCAAAGATGGTATCTC
CTATACCTTCTCCATCGTGCCGAACGCCCTGGGTAAAGACATGGAATCCCAGAAAGCCAACCTGGCTGGCCTGATGGACG
GTTACTTCCATCACGAGTCCAACCTGGAAGGTGGTCAGCACCTGAACGTGAACGTGATGAACCGTGAAATGCTGCTGGAC
GCCATGGAAAACCCGGAGAAATATCCGCAGTTGACCATCCGCGTATCCGGTTATGCCGTTCGCTTCAACTCTTTGACCAA
AGAGCAACAGCAAGACGTCATTACCCGTACCTTCACCGCTTCTCTGTAA

Upstream 100 bases:

>100_bases
CATTGGTGGTGGCGTCATGGTTGGTCTGACTTACTGGTTTATCTTCCGTCGTCATCATTAAGGATGATTGCGGGATACTA
CATCAATACCGTGTGAGGTA

Downstream 100 bases:

>100_bases
TCGCTTATTGGTAAGCAAGAGTCGCAGTGACTAAAAACCGGCGCCCAGGCGCCGGTTTTTTTATGTCTGCTTGCCAGCAG
GCATCCACTCGAAAGGGATC

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 762; Mature: 762

Protein sequence:

>762_residues
MKMAELNEQFQKAWEGFAAGEWQTSVNTRDFIQKNYTPYEGDESFLAGATEATTKLWDKVMEGIKIENRTHAPVDFDTDL
PSTITAHDAGYIDQSLEQIVGLQTDKPLKRAIIANGGIKMVKTSCEVYGRQLDPMVEKIFTEYRKTHNQGVFDVYTKDIL
NCRKSGVITGLPDAYGRGRIIGDYRRVALYGIDFLMADKLAQFKSLQADLENGVNLEATIRLREEISEQHRALAQMKVMA
AKYGCDISVPAKNAKEAVQWTYFAYLAAVKSQNGAAMSFGRTSSFLDVYIERDLKKGVLTEQEAQELMDHMVMKLRMVRF
LRTPEYDSLFSGDPMWATETLAGMGVDGRTLVTKNSFRMLNTLYTMGPSPEPNLTILWSEQLPIGFKKYCAKVSIETSSV
QYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGMDEKLKIQIGPKTEMVKSEYLDYNDVMD
RLDHFMDWLAKQYVAALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRDEDGVAIDF
EIDGEYPQFGNNDARVDDIACDLVERFMKKIQKLNTYRGAVATQSVLTITSNVVYGKKTGNTPDGRRSGAPFGPGANPMH
GRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDMESQKANLAGLMDGYFHHESNLEGGQHLNVNVMNREMLLD
AMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTASL

Sequences:

>Translated_762_residues
MKMAELNEQFQKAWEGFAAGEWQTSVNTRDFIQKNYTPYEGDESFLAGATEATTKLWDKVMEGIKIENRTHAPVDFDTDL
PSTITAHDAGYIDQSLEQIVGLQTDKPLKRAIIANGGIKMVKTSCEVYGRQLDPMVEKIFTEYRKTHNQGVFDVYTKDIL
NCRKSGVITGLPDAYGRGRIIGDYRRVALYGIDFLMADKLAQFKSLQADLENGVNLEATIRLREEISEQHRALAQMKVMA
AKYGCDISVPAKNAKEAVQWTYFAYLAAVKSQNGAAMSFGRTSSFLDVYIERDLKKGVLTEQEAQELMDHMVMKLRMVRF
LRTPEYDSLFSGDPMWATETLAGMGVDGRTLVTKNSFRMLNTLYTMGPSPEPNLTILWSEQLPIGFKKYCAKVSIETSSV
QYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGMDEKLKIQIGPKTEMVKSEYLDYNDVMD
RLDHFMDWLAKQYVAALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRDEDGVAIDF
EIDGEYPQFGNNDARVDDIACDLVERFMKKIQKLNTYRGAVATQSVLTITSNVVYGKKTGNTPDGRRSGAPFGPGANPMH
GRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDMESQKANLAGLMDGYFHHESNLEGGQHLNVNVMNREMLLD
AMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTASL
>Mature_762_residues
MKMAELNEQFQKAWEGFAAGEWQTSVNTRDFIQKNYTPYEGDESFLAGATEATTKLWDKVMEGIKIENRTHAPVDFDTDL
PSTITAHDAGYIDQSLEQIVGLQTDKPLKRAIIANGGIKMVKTSCEVYGRQLDPMVEKIFTEYRKTHNQGVFDVYTKDIL
NCRKSGVITGLPDAYGRGRIIGDYRRVALYGIDFLMADKLAQFKSLQADLENGVNLEATIRLREEISEQHRALAQMKVMA
AKYGCDISVPAKNAKEAVQWTYFAYLAAVKSQNGAAMSFGRTSSFLDVYIERDLKKGVLTEQEAQELMDHMVMKLRMVRF
LRTPEYDSLFSGDPMWATETLAGMGVDGRTLVTKNSFRMLNTLYTMGPSPEPNLTILWSEQLPIGFKKYCAKVSIETSSV
QYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGMDEKLKIQIGPKTEMVKSEYLDYNDVMD
RLDHFMDWLAKQYVAALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRDEDGVAIDF
EIDGEYPQFGNNDARVDDIACDLVERFMKKIQKLNTYRGAVATQSVLTITSNVVYGKKTGNTPDGRRSGAPFGPGANPMH
GRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDMESQKANLAGLMDGYFHHESNLEGGQHLNVNVMNREMLLD
AMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTASL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=760, Percent_Identity=85.3947368421053, Blast_Score=1396, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=753, Percent_Identity=77.2908366533864, Blast_Score=1256, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=592, Percent_Identity=26.0135135135135, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1790388, Length=642, Percent_Identity=24.9221183800623, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=78.125, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85396; Mature: 85396

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMAELNEQFQKAWEGFAAGEWQTSVNTRDFIQKNYTPYEGDESFLAGATEATTKLWDKV
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHH
MEGIKIENRTHAPVDFDTDLPSTITAHDAGYIDQSLEQIVGLQTDKPLKRAIIANGGIKM
HHCCEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEE
VKTSCEVYGRQLDPMVEKIFTEYRKTHNQGVFDVYTKDILNCRKSGVITGLPDAYGRGRI
EEHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCE
IGDYRRVALYGIDFLMADKLAQFKSLQADLENGVNLEATIRLREEISEQHRALAQMKVMA
EHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
AKYGCDISVPAKNAKEAVQWTYFAYLAAVKSQNGAAMSFGRTSSFLDVYIERDLKKGVLT
HHHCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCC
EQEAQELMDHMVMKLRMVRFLRTPEYDSLFSGDPMWATETLAGMGVDGRTLVTKNSFRML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCHHHHH
NTLYTMGPSPEPNLTILWSEQLPIGFKKYCAKVSIETSSVQYENDDLMRPDFNNDDYAIA
HHHHHCCCCCCCCEEEEECCCCCCCHHHHHHEEEEECCCEEECCCCCCCCCCCCCCCEEE
CCVSPMIVGKQMQFFGARANLAKTMLYAINGGMDEKLKIQIGPKTEMVKSEYLDYNDVMD
EECCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCHHHHHH
RLDHFMDWLAKQYVAALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AIKYAKVKPVRDEDGVAIDFEIDGEYPQFGNNDARVDDIACDLVERFMKKIQKLNTYRGA
HHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCH
VATQSVLTITSNVVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFA
HHHHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
YAKDGISYTFSIVPNALGKDMESQKANLAGLMDGYFHHESNLEGGQHLNVNVMNREMLLD
HHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHH
AMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTASL
HHHCCCCCCEEEEEEECEEEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKMAELNEQFQKAWEGFAAGEWQTSVNTRDFIQKNYTPYEGDESFLAGATEATTKLWDKV
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHH
MEGIKIENRTHAPVDFDTDLPSTITAHDAGYIDQSLEQIVGLQTDKPLKRAIIANGGIKM
HHCCEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEE
VKTSCEVYGRQLDPMVEKIFTEYRKTHNQGVFDVYTKDILNCRKSGVITGLPDAYGRGRI
EEHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCE
IGDYRRVALYGIDFLMADKLAQFKSLQADLENGVNLEATIRLREEISEQHRALAQMKVMA
EHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
AKYGCDISVPAKNAKEAVQWTYFAYLAAVKSQNGAAMSFGRTSSFLDVYIERDLKKGVLT
HHHCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCC
EQEAQELMDHMVMKLRMVRFLRTPEYDSLFSGDPMWATETLAGMGVDGRTLVTKNSFRML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCHHHHH
NTLYTMGPSPEPNLTILWSEQLPIGFKKYCAKVSIETSSVQYENDDLMRPDFNNDDYAIA
HHHHHCCCCCCCCEEEEECCCCCCCHHHHHHEEEEECCCEEECCCCCCCCCCCCCCCEEE
CCVSPMIVGKQMQFFGARANLAKTMLYAINGGMDEKLKIQIGPKTEMVKSEYLDYNDVMD
EECCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCHHHHHH
RLDHFMDWLAKQYVAALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AIKYAKVKPVRDEDGVAIDFEIDGEYPQFGNNDARVDDIACDLVERFMKKIQKLNTYRGA
HHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCH
VATQSVLTITSNVVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFA
HHHHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
YAKDGISYTFSIVPNALGKDMESQKANLAGLMDGYFHHESNLEGGQHLNVNVMNREMLLD
HHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHH
AMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTASL
HHHCCCCCCEEEEEEECEEEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]