Click here to switch to the map view.

The map label for this gene is gltA [H]

Identifier: 145299318

GI number: 145299318

Start: 2515764

End: 2517050

Strand: Direct

Name: gltA [H]

Synonym: ASA_2362

Alternate gene names: 145299318

Gene position: 2515764-2517050 (Clockwise)

Preceding gene: 145299308

Following gene: 145299319

Centisome position: 53.5

GC content: 57.58

Gene sequence:

>1287_bases
ATGGCTGATAAGATAGCCACCCTACATCTGCCCGGTAAAGACCCGGTAGAACTACCGATCCTGTCAGGGACTGTCGGACC
CGACGTGATTGACGTCAGAAAATTGGGCGCGCAAGGGTACTTCACCTTTGACCCCGGATTTATGGCAACCGGTGCTTGTA
AATCATCCATTACCTATATCGACGGAGATCAGGGTGTCTTGCTGCACCGTGGTTACCCCATCGCCCAGTTGGCCACCCAG
GCCAGCTACCTCGAAGTCTGCTATATCCTGCTGTACGGCGAGGCACCTACCAAGGCGCAGTACACGGAATTCGATCGTCT
GGTCACCCGCCATACCATGGTGCACGAACAGATCGCCTTCTTCTTCCGCGGCTTCCGTCGTGACTCCCACCCCATGGCCA
TCATGTGCGGCGTGGTCAGTGCGCTGTCTGCCTTCTATCACGATGCGCTCGACATCAACAATGAAGAGCATCGCGAGATC
TGTGCCTTCCGTCTGCTCTCCAAGATGCCGACCCTGGCCGCCATGTGCTACAAGTACTCCATCGGTCAGCCGTTCATGCA
GCCGCGTAACGCCCTCTCCTATGCCGGCAACTTCCTGCACATGATGTTTGGCGTGCCGACCGAAGAGTACAAGGTCAATC
CCATCGTCGAACGCGCCATGGACCGCATCTTTACCCTGCATGCGGATCACGAGCAAAACGCCTCCACCTCCACAGTGCGT
CTGGCCGGCTCCTCCGGTGCCAACCCGTTTGCCTGTATTGCCGCCGGGATCGCCTCCCTGTGGGGTCCGGCCCACGGTGG
TGCCAACGAAGCTTGCCTCAAGATGCTCGAAGAGATCGGCTCGGTGGAACGCATTCCCGAGTACATCGCCAAGGCCAAGG
ACAAGAATGATCCCTTCCGTCTGATGGGTTTCGGTCACCGTGTCTACAAGAACCACGATCCCCGTGCCCAGATGATGCGT
CAGACCTGTCACGAAGTGCTGGAAGAGCTGCAGATCAAGGATCCGCTGCTGGAAGTGGCCATGGAGCTGGAACGCATTGC
GCTGTCCGACCCCTACTTCATCGAGAAGAAGCTCTACCCGAACGTGGACTTCTACTCCGGCATCATCATGAAGGCCATCG
GCATCCCCATGTCCATGTTCACCGTGATCTTCGCCATCTCCCGTACCATCGGCTGGATTGCCCACTGGAACGAAATGCAC
TCGGATCCGGATCAGAAGATCGGTCGTCCGCGTCAGCTCTATACAGGGCAACCACTGCGGGAATTCACTCCTATCGATCA
GCGCTGA

Upstream 100 bases:

>100_bases
CTCCAGCCGCCCTGGTACGGGTCAGAACCTTTGCCAGCGTCATCAAACTCGTAATAAAGTAGAGCTGGAACCAATTATAA
AAGAGCAAAGGAGACGTGCT

Downstream 100 bases:

>100_bases
TGCCCTGAGTCGACATGGTTTGTTACCAAGGCGGTCATTGACCGCCTTTTTATTTGCCGCTCTAATGCCCCTTTTCGATC
ACAAAGGAGTCCATCATGAG

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MADKIATLHLPGKDPVELPILSGTVGPDVIDVRKLGAQGYFTFDPGFMATGACKSSITYIDGDQGVLLHRGYPIAQLATQ
ASYLEVCYILLYGEAPTKAQYTEFDRLVTRHTMVHEQIAFFFRGFRRDSHPMAIMCGVVSALSAFYHDALDINNEEHREI
CAFRLLSKMPTLAAMCYKYSIGQPFMQPRNALSYAGNFLHMMFGVPTEEYKVNPIVERAMDRIFTLHADHEQNASTSTVR
LAGSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIGSVERIPEYIAKAKDKNDPFRLMGFGHRVYKNHDPRAQMMR
QTCHEVLEELQIKDPLLEVAMELERIALSDPYFIEKKLYPNVDFYSGIIMKAIGIPMSMFTVIFAISRTIGWIAHWNEMH
SDPDQKIGRPRQLYTGQPLREFTPIDQR

Sequences:

>Translated_428_residues
MADKIATLHLPGKDPVELPILSGTVGPDVIDVRKLGAQGYFTFDPGFMATGACKSSITYIDGDQGVLLHRGYPIAQLATQ
ASYLEVCYILLYGEAPTKAQYTEFDRLVTRHTMVHEQIAFFFRGFRRDSHPMAIMCGVVSALSAFYHDALDINNEEHREI
CAFRLLSKMPTLAAMCYKYSIGQPFMQPRNALSYAGNFLHMMFGVPTEEYKVNPIVERAMDRIFTLHADHEQNASTSTVR
LAGSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIGSVERIPEYIAKAKDKNDPFRLMGFGHRVYKNHDPRAQMMR
QTCHEVLEELQIKDPLLEVAMELERIALSDPYFIEKKLYPNVDFYSGIIMKAIGIPMSMFTVIFAISRTIGWIAHWNEMH
SDPDQKIGRPRQLYTGQPLREFTPIDQR
>Mature_427_residues
ADKIATLHLPGKDPVELPILSGTVGPDVIDVRKLGAQGYFTFDPGFMATGACKSSITYIDGDQGVLLHRGYPIAQLATQA
SYLEVCYILLYGEAPTKAQYTEFDRLVTRHTMVHEQIAFFFRGFRRDSHPMAIMCGVVSALSAFYHDALDINNEEHREIC
AFRLLSKMPTLAAMCYKYSIGQPFMQPRNALSYAGNFLHMMFGVPTEEYKVNPIVERAMDRIFTLHADHEQNASTSTVRL
AGSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIGSVERIPEYIAKAKDKNDPFRLMGFGHRVYKNHDPRAQMMRQ
TCHEVLEELQIKDPLLEVAMELERIALSDPYFIEKKLYPNVDFYSGIIMKAIGIPMSMFTVIFAISRTIGWIAHWNEMHS
DPDQKIGRPRQLYTGQPLREFTPIDQR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=353, Percent_Identity=26.628895184136, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786939, Length=422, Percent_Identity=75.3554502369668, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1786527, Length=371, Percent_Identity=30.9973045822102, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17555174, Length=372, Percent_Identity=26.0752688172043, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324328, Length=344, Percent_Identity=27.3255813953488, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319850, Length=347, Percent_Identity=27.9538904899135, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325257, Length=366, Percent_Identity=27.5956284153005, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640126, Length=404, Percent_Identity=27.7227722772277, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24640124, Length=404, Percent_Identity=27.7227722772277, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI21356863, Length=373, Percent_Identity=25.4691689008043, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48141; Mature: 48010

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKIATLHLPGKDPVELPILSGTVGPDVIDVRKLGAQGYFTFDPGFMATGACKSSITYI
CCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHCCCEEE
DGDQGVLLHRGYPIAQLATQASYLEVCYILLYGEAPTKAQYTEFDRLVTRHTMVHEQIAF
ECCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
FFRGFRRDSHPMAIMCGVVSALSAFYHDALDINNEEHREICAFRLLSKMPTLAAMCYKYS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
IGQPFMQPRNALSYAGNFLHMMFGVPTEEYKVNPIVERAMDRIFTLHADHEQNASTSTVR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIGSVERIPEYIAKAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
LMGFGHRVYKNHDPRAQMMRQTCHEVLEELQIKDPLLEVAMELERIALSDPYFIEKKLYP
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHHHCCC
NVDFYSGIIMKAIGIPMSMFTVIFAISRTIGWIAHWNEMHSDPDQKIGRPRQLYTGQPLR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHH
EFTPIDQR
HCCCCCCC
>Mature Secondary Structure 
ADKIATLHLPGKDPVELPILSGTVGPDVIDVRKLGAQGYFTFDPGFMATGACKSSITYI
CCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHCCCEEE
DGDQGVLLHRGYPIAQLATQASYLEVCYILLYGEAPTKAQYTEFDRLVTRHTMVHEQIAF
ECCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
FFRGFRRDSHPMAIMCGVVSALSAFYHDALDINNEEHREICAFRLLSKMPTLAAMCYKYS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
IGQPFMQPRNALSYAGNFLHMMFGVPTEEYKVNPIVERAMDRIFTLHADHEQNASTSTVR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIGSVERIPEYIAKAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
LMGFGHRVYKNHDPRAQMMRQTCHEVLEELQIKDPLLEVAMELERIALSDPYFIEKKLYP
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHHHCCC
NVDFYSGIIMKAIGIPMSMFTVIFAISRTIGWIAHWNEMHSDPDQKIGRPRQLYTGQPLR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHH
EFTPIDQR
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]