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The map label for this gene is focA [H]

Identifier: 145299307

GI number: 145299307

Start: 2501418

End: 2502266

Strand: Direct

Name: focA [H]

Synonym: ASA_2351

Alternate gene names: 145299307

Gene position: 2501418-2502266 (Clockwise)

Preceding gene: 145299306

Following gene: 145299308

Centisome position: 53.19

GC content: 56.54

Gene sequence:

>849_bases
TTGAAAGCAGAATCTCCCTTTGATTGTCTCAAGCCAGAAGCCATCGCAGCATTGGCAGAAGACATCACCTATGCCAAGGC
TACCAAGCCTGCACACAAGGTCATCCCCCTTGCCATCACAGCAGGCGCTTTCATCGCCATCGCGTTTGTCTTTTTCATCA
CTGTCACCACGGGTGCCGGCGATGTGGCCTGGGGCCTCAGCAAACTGGTCGGCGGCCTCTGTTTCTCCCTCGGTCTTATT
CTGTGTGTGCTGCTCGGTGCCGAGTTGTTCACCTCCACGACCCTGACGCTGGTGGCCAAGGCCGCCAATCGCATCACCTG
GGGTCAACTGCTCAAGAACTGGGGGCTGGTCTACTTCGGCAACCTCATCGGCGGTCTCATCATAGTCGCGCTCATCATCA
TGTCGGCCGAGTACACGGCGGCAAATGGCCAATGGGGCCTCAATGCCCTGAAAGTGGCCCAGCACAAGATCCACCACACC
TTCTTCGAGGCCCTCGCCCTCGGTATTCTCTGCAACCTCATGGTGTGTCTCGCCGTGTGGATGGCCTTCGGTGCCCGCAG
TGCAACCGACAAGGTGATGGTCATGCTGCTCCCGGTGGCCATGTTCGTGGCATCCGGTTTCGAGCACAGCATCGCAAACA
TGTTTATGATCCCGGTCGGTATCGCTATCCATAGCGTCGCAGGCCCGGAATTTTGGCAGGCCATCGGCCAGGATCCGGCG
ACCTTTGCCGATCTGACCGTATCCAACTTCGTCCTACACAACCTCATTCCTGTCACCATAGGTAATATCATTGGTGGTGG
CGTCATGGTTGGTCTGACTTACTGGTTTATCTTCCGTCGTCATCATTAA

Upstream 100 bases:

>100_bases
TAAAATTACATTCACAATCTGCAATTCTTGATCGGAGTCAATTTTGACGATCTGGCCCTTTGTTATGATTTCCACAGGCA
AAACAGCAGGTACATGAGAA

Downstream 100 bases:

>100_bases
GGATGATTGCGGGATACTACATCAATACCGTGTGAGGTAATGAAAATGGCAGAACTTAACGAACAGTTCCAAAAAGCATG
GGAAGGCTTCGCGGCCGGTG

Product: formate transporter 1 FocA

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKAESPFDCLKPEAIAALAEDITYAKATKPAHKVIPLAITAGAFIAIAFVFFITVTTGAGDVAWGLSKLVGGLCFSLGLI
LCVLLGAELFTSTTLTLVAKAANRITWGQLLKNWGLVYFGNLIGGLIIVALIIMSAEYTAANGQWGLNALKVAQHKIHHT
FFEALALGILCNLMVCLAVWMAFGARSATDKVMVMLLPVAMFVASGFEHSIANMFMIPVGIAIHSVAGPEFWQAIGQDPA
TFADLTVSNFVLHNLIPVTIGNIIGGGVMVGLTYWFIFRRHH

Sequences:

>Translated_282_residues
MKAESPFDCLKPEAIAALAEDITYAKATKPAHKVIPLAITAGAFIAIAFVFFITVTTGAGDVAWGLSKLVGGLCFSLGLI
LCVLLGAELFTSTTLTLVAKAANRITWGQLLKNWGLVYFGNLIGGLIIVALIIMSAEYTAANGQWGLNALKVAQHKIHHT
FFEALALGILCNLMVCLAVWMAFGARSATDKVMVMLLPVAMFVASGFEHSIANMFMIPVGIAIHSVAGPEFWQAIGQDPA
TFADLTVSNFVLHNLIPVTIGNIIGGGVMVGLTYWFIFRRHH
>Mature_282_residues
MKAESPFDCLKPEAIAALAEDITYAKATKPAHKVIPLAITAGAFIAIAFVFFITVTTGAGDVAWGLSKLVGGLCFSLGLI
LCVLLGAELFTSTTLTLVAKAANRITWGQLLKNWGLVYFGNLIGGLIIVALIIMSAEYTAANGQWGLNALKVAQHKIHHT
FFEALALGILCNLMVCLAVWMAFGARSATDKVMVMLLPVAMFVASGFEHSIANMFMIPVGIAIHSVAGPEFWQAIGQDPA
TFADLTVSNFVLHNLIPVTIGNIIGGGVMVGLTYWFIFRRHH

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=285, Percent_Identity=65.6140350877193, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1788837, Length=282, Percent_Identity=45.0354609929078, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI87082249, Length=275, Percent_Identity=29.0909090909091, Blast_Score=82, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321779, Length=209, Percent_Identity=25.3588516746411, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 30149; Mature: 30149

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]