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The map label for this gene is metK [H]

Identifier: 145295725

GI number: 145295725

Start: 1837785

End: 1838558

Strand: Reverse

Name: metK [H]

Synonym: cgR_1652

Alternate gene names: 145295725

Gene position: 1838558-1837785 (Counterclockwise)

Preceding gene: 145295726

Following gene: 145295724

Centisome position: 55.48

GC content: 55.43

Gene sequence:

>774_bases
GTGGCTCAGCCAACCGCCGTCCGTTTGTTCACCAGTGAATCTGTAACTGAGGGACATCCAGACAAAATATGTGATGCTAT
TTCCGATACCATTTTGGACGCGCTGCTCGAAAAAGATCCGCAGTCGCGCGTCGCAGTGGAAACTGTGGTCACCACCGGAA
TCGTCCATGTTGTTGGCGAGGTCCGTACCAGCGCTTACGTAGAGATCCCTCAATTAGTCCGCAACAAGCTCATCGAAATC
GGATTCAACTCCTCTGAGGTTGGATTCGACGGACGCACCTGTGGCGTCTCAGTATCTATCGGTGAGCAGTCCCAGGAAAT
CGCTGACGGCGTGGATAACTCCGACGAAGCCCGCACCAACGGCGACGTTGAAGAAGACGACCGCGCAGGTGCTGGCGACC
AGGGCCTGATGTTCGGCTACGCCACCAACGAAACCGAAGAGTACATGCCTCTTCCTATCGCGTTGGCGCACCGACTGTCA
CGTCGTCTGACCCAGGTTCGTAAAGAGGGTATCGTTCCTCACCTGCGTCCAGACGGAAAAACCCAGGTCACCTTCGCATA
CGATGCGCAAGACCGCCCTAGTCACCTAGATACCGTTGTCATCTCCACCCAGCACGACCCAGAAGTTGACCGTGCATGGT
TGGAAACCCAGCTGCGCGAACACGTCATTGATTGGGTAATCAAAGACGCAGGCATTGAGGATCTGGCAACCGGTGAGATC
ACCGTGTTGATCAACCCCTTCAGGTTCCTTCATTCTGGGTGGCCCCATGGGTGA

Upstream 100 bases:

>100_bases
CAGCTAGACCACTGACATTGCAGTTTTAGACAGCTTGGTCTATATTGGTTTTTTGTATTTAAGACTATTTATTCTCAACT
TCTTCGAAAGAAGGGTATTT

Downstream 100 bases:

>100_bases
TGCGGGTCTGACCGGCCGCAAGATCATCGTGGATACCTACGGTGGCATGGCTCGCCATGGTGGTGGAGCATTCTCCGGTA
AGGATCCAAGCAAGGTGGAC

Product: hypothetical protein

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MAQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGEVRTSAYVEIPQLVRNKLIEI
GFNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTNGDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLS
RRLTQVRKEGIVPHLRPDGKTQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEI
TVLINPFRFLHSGWPHG

Sequences:

>Translated_257_residues
MAQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGEVRTSAYVEIPQLVRNKLIEI
GFNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTNGDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLS
RRLTQVRKEGIVPHLRPDGKTQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEI
TVLINPFRFLHSGWPHG
>Mature_256_residues
AQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGEVRTSAYVEIPQLVRNKLIEIG
FNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTNGDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLSR
RLTQVRKEGIVPHLRPDGKTQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEIT
VLINPFRFLHSGWPHG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=223, Percent_Identity=51.1210762331839, Blast_Score=207, Evalue=8e-54,
Organism=Homo sapiens, GI4557737, Length=226, Percent_Identity=48.6725663716814, Blast_Score=202, Evalue=2e-52,
Organism=Escherichia coli, GI1789311, Length=252, Percent_Identity=49.6031746031746, Blast_Score=226, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17538498, Length=257, Percent_Identity=46.3035019455253, Blast_Score=207, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17538494, Length=252, Percent_Identity=46.8253968253968, Blast_Score=207, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17551082, Length=252, Percent_Identity=46.8253968253968, Blast_Score=206, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI25145633, Length=252, Percent_Identity=45.2380952380952, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI32565905, Length=252, Percent_Identity=40.0793650793651, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI32565911, Length=207, Percent_Identity=42.0289855072464, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25145635, Length=207, Percent_Identity=40.0966183574879, Blast_Score=138, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32565903, Length=180, Percent_Identity=42.2222222222222, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=51.8072289156626, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320710, Length=252, Percent_Identity=46.4285714285714, Blast_Score=207, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323209, Length=252, Percent_Identity=45.6349206349206, Blast_Score=199, Evalue=3e-52,
Organism=Drosophila melanogaster, GI45552159, Length=255, Percent_Identity=41.9607843137255, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24580529, Length=255, Percent_Identity=41.9607843137255, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24580537, Length=255, Percent_Identity=41.9607843137255, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24580533, Length=255, Percent_Identity=41.9607843137255, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24580535, Length=255, Percent_Identity=41.9607843137255, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24580531, Length=255, Percent_Identity=41.9607843137255, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24580527, Length=255, Percent_Identity=43.1372549019608, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24580525, Length=255, Percent_Identity=43.1372549019608, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24580541, Length=240, Percent_Identity=40.8333333333333, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24580539, Length=129, Percent_Identity=48.8372093023256, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 28281; Mature: 28150

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00376 ADOMET_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGE
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VRTSAYVEIPQLVRNKLIEIGFNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTN
HHHHHHEEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCCCCCCCC
GDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLSRRLTQVRKEGIVPHLRPDGK
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEI
EEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
TVLINPFRFLHSGWPHG
EEEECCHHHHHCCCCCC
>Mature Secondary Structure 
AQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGE
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VRTSAYVEIPQLVRNKLIEIGFNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTN
HHHHHHEEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCCCCCCCC
GDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLSRRLTQVRKEGIVPHLRPDGK
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEI
EEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
TVLINPFRFLHSGWPHG
EEEECCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]