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The map label for this gene is metK [H]

Identifier: 145295724

GI number: 145295724

Start: 1837334

End: 1837792

Strand: Reverse

Name: metK [H]

Synonym: cgR_1651

Alternate gene names: 145295724

Gene position: 1837792-1837334 (Counterclockwise)

Preceding gene: 145295725

Following gene: 145295723

Centisome position: 55.45

GC content: 56.43

Gene sequence:

>459_bases
ATGGGTGATGCGGGTCTGACCGGCCGCAAGATCATCGTGGATACCTACGGTGGCATGGCTCGCCATGGTGGTGGAGCATT
CTCCGGTAAGGATCCAAGCAAGGTGGACCGCTCTGCTGCATACGCCATGCGTTGGGTAGCAAAGAACATCGTGGCAGCAG
GCCTTGCTGATCGCGCTGAAGTTCAGGTTGCATACGCCATTGGACGCGCAAAGCCAGTCGGACTTTACGTTGAAACCTTT
GACACCAACAAGGAAGGCCTGAGCGACGAGCAGATTCAGGCTGCCGTGTTGGAGGTCTTTGACCTGCGTCCAGCAGCAAT
TATCCGTGAGCTTGATCTGCTTCGTCCGATCTACGCTGACACTGCTGCCTACGGCCACTTTGGTCGCACTGATTTGGACC
TTCCTTGGGAGGCTATCGACCGCGTTGATGAACTTCGCGCAGCCCTCAAGTTGGCCTAA

Upstream 100 bases:

>100_bases
ATTGATTGGGTAATCAAAGACGCAGGCATTGAGGATCTGGCAACCGGTGAGATCACCGTGTTGATCAACCCCTTCAGGTT
CCTTCATTCTGGGTGGCCCC

Downstream 100 bases:

>100_bases
AAATCTGATGTAGTATCTTCGGATTCATGGCAAAAACCCGCGTCCCCGCTCCTGAAAAGTCGGTGGCGCGGGTTTTACCT
CTTTTGGGGTTACCTCACCT

Product: hypothetical protein

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLADRAEVQVAYAIGRAKPVGLYVETF
DTNKEGLSDEQIQAAVLEVFDLRPAAIIRELDLLRPIYADTAAYGHFGRTDLDLPWEAIDRVDELRAALKLA

Sequences:

>Translated_152_residues
MGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLADRAEVQVAYAIGRAKPVGLYVETF
DTNKEGLSDEQIQAAVLEVFDLRPAAIIRELDLLRPIYADTAAYGHFGRTDLDLPWEAIDRVDELRAALKLA
>Mature_151_residues
GDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLADRAEVQVAYAIGRAKPVGLYVETFD
TNKEGLSDEQIQAAVLEVFDLRPAAIIRELDLLRPIYADTAAYGHFGRTDLDLPWEAIDRVDELRAALKLA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=136, Percent_Identity=59.5588235294118, Blast_Score=147, Evalue=3e-36,
Organism=Homo sapiens, GI5174529, Length=136, Percent_Identity=59.5588235294118, Blast_Score=142, Evalue=9e-35,
Organism=Escherichia coli, GI1789311, Length=151, Percent_Identity=68.8741721854305, Blast_Score=203, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17538498, Length=137, Percent_Identity=64.2335766423358, Blast_Score=155, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17551082, Length=137, Percent_Identity=62.7737226277372, Blast_Score=155, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32565911, Length=137, Percent_Identity=64.2335766423358, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25145633, Length=137, Percent_Identity=62.7737226277372, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538494, Length=137, Percent_Identity=61.3138686131387, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32565905, Length=137, Percent_Identity=61.3138686131387, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25145635, Length=137, Percent_Identity=62.7737226277372, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI32565903, Length=137, Percent_Identity=61.3138686131387, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6323209, Length=137, Percent_Identity=62.043795620438, Blast_Score=152, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320710, Length=142, Percent_Identity=58.4507042253521, Blast_Score=148, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45552159, Length=137, Percent_Identity=58.3941605839416, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24580529, Length=137, Percent_Identity=58.3941605839416, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24580537, Length=137, Percent_Identity=58.3941605839416, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24580533, Length=137, Percent_Identity=58.3941605839416, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24580535, Length=137, Percent_Identity=58.3941605839416, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24580531, Length=137, Percent_Identity=58.3941605839416, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24580541, Length=137, Percent_Identity=58.3941605839416, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24580527, Length=137, Percent_Identity=58.3941605839416, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24580525, Length=137, Percent_Identity=58.3941605839416, Blast_Score=139, Evalue=6e-34,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 16421; Mature: 16290

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLADRAE
CCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VQVAYAIGRAKPVGLYVETFDTNKEGLSDEQIQAAVLEVFDLRPAAIIRELDLLRPIYAD
EEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TAAYGHFGRTDLDLPWEAIDRVDELRAALKLA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLADRAE
CCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VQVAYAIGRAKPVGLYVETFDTNKEGLSDEQIQAAVLEVFDLRPAAIIRELDLLRPIYAD
EEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TAAYGHFGRTDLDLPWEAIDRVDELRAALKLA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]