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The map label for this gene is metG [H]

Identifier: 145295063

GI number: 145295063

Start: 1111388

End: 1113220

Strand: Direct

Name: metG [H]

Synonym: cgR_1006

Alternate gene names: 145295063

Gene position: 1111388-1113220 (Clockwise)

Preceding gene: 145295062

Following gene: 145295064

Centisome position: 33.53

GC content: 56.03

Gene sequence:

>1833_bases
ATGACGAAGAACGTGCTCGTATCTGTTGCCTGGCCGTATGCCAACGGACCCCGTCACATTGGACATGTGGCGGGGTTTGG
TGTCCCCTCCGATGTGTTCGCAAGGTTCCAGCGAATGTCTGGCAACAACGTGCTCATGGTGTCCGGCACCGATGAGCACG
GCACGCCACTTCTGGTTCAAGCAGACAAAGAAGGCGTCACCGTTCAAGACCTCGCGGATAAGTACAACCGCCAGATCGTC
GAAGACCTCACCGGCCTGGGCCTGTCCTATGACCTTTTCACCCGCACCACCACCTCCAACCACTACGCAGTAGTGCAGGA
ACTGTTCCGTGGTCTGTACGACAACGGTTACATGATCAAGGAAACCACCCTCGGTGCGATTTCCCCATCCACTGGCCGTA
CCCTGCCAGACCGCTACATTGAAGGCACCTGCCCAATCTGTGGCACCGACGGCGCTCGTGGCGACCAGTGCGACAACTGC
GGAAACCAGCTCGATCCAGCGGACCTGATCAACCCGGTATCCAAGATCAACGGCGAAACCCCAGAGTTCGTTGAGACCGA
ACACTTCCTGCTCGACCTGCCAGCACTAGCTGAAGCACTAACCGAGTGGCTGAAGGGACGCGAAGACTGGCGTCCAAACG
TGTTGAAGTTCTCGCTCAACCTGCTGGACGATATCCGCCCACGCGCAATGTCCCGCGATATCGACTGGGGCATCCCAATC
CCAGTTGAAGGATGGCAAGACAACAACGCCAAGAAGCTCTACGTTTGGTTTGACGCTGTCGTTGGCTACTTGTCCGCATC
AATCGAATGGGCCTATCGCTCCGGCGACCCAGAAGCATGGCGCACCTTCTGGAATGATCCAGAAACCAAGTCCTACTACT
TCATGGGCAAAGACAACATCACCTTCCACTCCCAGATCTGGCCAGCTGAACTTCTCGGCTACGCAGGTAAGGGCTCCCGA
GGTGGAGAGATCGGCGACCTGGGTGTTCTGAACCTGCCTACTGAGGTTGTTTCCTCTGAGTTCCTGACTATGTCTGGATC
CAAGTTCTCCTCATCCAAGGGCGTTGTCATCTACGTGAAGGACTTCCTCAAGGAGTTCGGCCCAGATGCGCTGCGATACT
TCATCGCTGTCGCAGGCCCAGAAAACAACGACACCGACTTCACCTGGGATGAATTTGTCCGCCGCGTAAACAACGAGCTG
GCAAACGGCTGGGGCAACCTGGTCAACCGTACTGTATCCATGGCGCACAAGAACTTCGGTGAAGTACCAGTACCTGGCGC
ACTGGAAGAATCTGACAAGAAGATCCTTGATCTTGCTACCGCTGCCTTTGAATCCGTTGCTGCGAACCTGGATCAGTCCA
AGTTCAAGGCCGGTATCTCTGAAATCATGCACGTTGTCGGTGAGGCCAACGCCTACATCGCAGAGCAAGAACCATGGAAG
CTTGCCAAGGATGACACCAAGCGCGAGCGTCTTGCCACCGTGCTGTGGACCGCGCTGCAGGTTGTTTCTGACTGCAACAC
CATGCTGACTCCATACCTGCCACACACTGCCCAAAAGGTTCATGAAACCTTGGGCCGTGATGGTATCTGGGCAGCATCAC
CACAGATCGTGGAAGTCACCAACGAATCACCACGCCAGCCAATCGGTGTGGGCCTGCCAGATCCAGAGCACACCTACCCA
GTAATCATGGGCGATTACAAGACCCAGCTGGCTAAGTGGCAGCGCATCGACGTTGTGCCAGGCACCACCTTGGAGAAGCC
AGCACCGCTGATTGCTAAGCTCGATCCAGAACTTGGTGAAACCGGCCCAGAATGGGCACCAGTGCAGAACTAA

Upstream 100 bases:

>100_bases
CGACGCAGGGAGCGTAAGGCGAGTGGTGCGGGGAAGCGTCGATAGGCAATTTTAACCCCTGATACCCCTTTCCGGCCGGG
CATAAATTAAGGTGGTACGC

Downstream 100 bases:

>100_bases
AGCATCTTTAGCATGAACCGAGCAGGTTGATCAGAAACATCTGATTGACCTGCTCTTATTAGTTGGCGGGGGTGGGGCGG
CGAGTGCGTGTCCACCCCAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MTKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQADKEGVTVQDLADKYNRQIV
EDLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIKETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNC
GNQLDPADLINPVSKINGETPEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPI
PVEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNITFHSQIWPAELLGYAGKGSR
GGEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVKDFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNEL
ANGWGNLVNRTVSMAHKNFGEVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWK
LAKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAASPQIVEVTNESPRQPIGVGLPDPEHTYP
VIMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGETGPEWAPVQN

Sequences:

>Translated_610_residues
MTKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQADKEGVTVQDLADKYNRQIV
EDLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIKETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNC
GNQLDPADLINPVSKINGETPEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPI
PVEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNITFHSQIWPAELLGYAGKGSR
GGEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVKDFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNEL
ANGWGNLVNRTVSMAHKNFGEVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWK
LAKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAASPQIVEVTNESPRQPIGVGLPDPEHTYP
VIMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGETGPEWAPVQN
>Mature_609_residues
TKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQADKEGVTVQDLADKYNRQIVE
DLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIKETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNCG
NQLDPADLINPVSKINGETPEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPIP
VEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNITFHSQIWPAELLGYAGKGSRG
GEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVKDFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNELA
NGWGNLVNRTVSMAHKNFGEVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWKL
AKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAASPQIVEVTNESPRQPIGVGLPDPEHTYPV
IMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGETGPEWAPVQN

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=531, Percent_Identity=33.3333333333333, Blast_Score=331, Evalue=2e-90,
Organism=Homo sapiens, GI24308436, Length=539, Percent_Identity=25.974025974026, Blast_Score=162, Evalue=1e-39,
Organism=Escherichia coli, GI1788432, Length=534, Percent_Identity=29.9625468164794, Blast_Score=275, Evalue=7e-75,
Organism=Escherichia coli, GI1786861, Length=153, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=597, Percent_Identity=29.9832495812395, Blast_Score=294, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI212645096, Length=527, Percent_Identity=26.5654648956357, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71992781, Length=363, Percent_Identity=26.4462809917355, Blast_Score=122, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321704, Length=536, Percent_Identity=32.4626865671642, Blast_Score=306, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6321610, Length=549, Percent_Identity=22.5865209471767, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24655311, Length=529, Percent_Identity=32.5141776937618, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI21358483, Length=534, Percent_Identity=25.4681647940075, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 67606; Mature: 67474

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQ
CCCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEE
ADKEGVTVQDLADKYNRQIVEDLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHEEECCCCCHHHHHHHHHHHHCCCCEEEE
ETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNCGNQLDPADLINPVSKINGET
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPI
CCCCCCHHHEEECHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCHHHHCCCCCCCCCC
PVEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNI
CCCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCE
TFHSQIWPAELLGYAGKGSRGGEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVK
EEECCCCHHHHHCCCCCCCCCCCCCCCCEECCCHHHHCCHHEEECCCCCCCCCCEEEEHH
DFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNELANGWGNLVNRTVSMAHKNFG
HHHHHHCHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LAKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAASPQIVEVT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCEEEEC
NESPRQPIGVGLPDPEHTYPVIMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGE
CCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHEEECCCCCCCCCCCCHHEECCCHHCC
TGPEWAPVQN
CCCCCCCCCC
>Mature Secondary Structure 
TKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQ
CCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEE
ADKEGVTVQDLADKYNRQIVEDLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHEEECCCCCHHHHHHHHHHHHCCCCEEEE
ETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNCGNQLDPADLINPVSKINGET
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPI
CCCCCCHHHEEECHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCHHHHCCCCCCCCCC
PVEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNI
CCCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCE
TFHSQIWPAELLGYAGKGSRGGEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVK
EEECCCCHHHHHCCCCCCCCCCCCCCCCEECCCHHHHCCHHEEECCCCCCCCCCEEEEHH
DFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNELANGWGNLVNRTVSMAHKNFG
HHHHHHCHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LAKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAASPQIVEVT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCEEEEC
NESPRQPIGVGLPDPEHTYPVIMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGE
CCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHEEECCCCCCCCCCCCHHEECCCHHCC
TGPEWAPVQN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]