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The map label for this gene is menE [H]

Identifier: 123965438

GI number: 123965438

Start: 184982

End: 186190

Strand: Reverse

Name: menE [H]

Synonym: P9515_02031

Alternate gene names: 123965438

Gene position: 186190-184982 (Counterclockwise)

Preceding gene: 123965439

Following gene: 123965435

Centisome position: 10.93

GC content: 27.21

Gene sequence:

>1209_bases
ATGAAAAACAAAATTCATATTATTGAAGTTGAAAATAATGAAAAAGAATCTACGAAGAAAATTCTTAAAAACATTAAGGA
AAAGAAAATAATTTACATAAAAAATAGATCTCACGAATATGAAGAAATATATAAGGCTATTAATATAAGAGGACCAGCAG
TTATCTTAAATAGCAGTGGCAGTAGTGGAAAGCCTAGAAAATGTATTCATAAATTAGAAAATCTCTATTCGTCTGCTAAG
GCATCTGGAATTTGGCTTGAACGACAAGGATTTAAATTAAAACACTGTTTAGTTTTTAATACTTTGCCGTTAAATCATAT
CAGTGGATTGATGCCGCTTTTTAGAAGTCAAATTTGGGATTGTGAACATATCAATATTTCCCCAGCATTAATTAAAAACC
CCAAGGAATTAGTAGTCTTCACTAATAAGATAAAAAAAGATAAACATCATCTTATAACTTCATTAGTTCCAATACAATTA
AAAAGACTTTTATCTGAAAAAAATGGAATTGATTGGTTAAAAATTTTTGATTTAATATGGATTGGTGGTGCATCTCTATC
TATTGAAACTTCTCGAAAGTGCAGATTCGAAAAAATAAATTTAGCACCCTGTTACGGATCAACTGAAACTGCCGCTATGG
TGACAAGCTTAAAACCTAAAGAATTTTTAGAAGGGCTTGATAATGTTGGGGAAATATTAACTGATATAAAATTAAGAATT
AGTAAAAATGGTTTAATCCAAATTCAATCAGACAGAATAGGAATTGAATTGCTGGAATCATATCAAACTAAAAATTTCAA
AGATAAAAATGGGTGGTGGGAAAGCAGTGATTCCGGAGAAATCCAAAAATTGAATAATTCTCTGTATTTGAAATTTATTC
GAAGAACAGATAATGCTTTTAATTCTGGAGGGGAAATAGTTTTTCCAGAAGTCATTAAATTACGATTAAATGAATTTATC
CTAAATAAAAAAATTCCAATAGAAAATTTCATAATTTCCAACATACCTAATAAAGAATGGGGAAATAAAATGGTAATCAT
TATTAATTTCAAAAAACAAACTGATAAAACAAATATTGAATATTCATTAAAACTATTAAAAAACTTTTCTAAAAATTGGC
CTAAACATGAGAGGCCGAAAAATTGGATTATTGGTAAACCTAAAAAAGATGATATTAAAAATTCAAATTACATGTTTACA
AAATTATAA

Upstream 100 bases:

>100_bases
AGTTATTGGGACCAACTCCAAAAGTTCCTGGTTTGGCTTTAAAAAAAAATCCCAATTCTTTTCTTTTTCTAAATAAGGCA
CAAAAGATATGGGATCAACT

Downstream 100 bases:

>100_bases
ATAATAATTAGCACTCTTTTAAATGAGTATTTATATTCGAAGCCTCTATCCATCTCTCGAGTTGATCAGGTAATTTAACC
TTCAATTTTGAATTCATATC

Product: putative O-succinylbenzoic acid--CoA ligase (OSB-CoA synthetase)

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKNKIHIIEVENNEKESTKKILKNIKEKKIIYIKNRSHEYEEIYKAINIRGPAVILNSSGSSGKPRKCIHKLENLYSSAK
ASGIWLERQGFKLKHCLVFNTLPLNHISGLMPLFRSQIWDCEHINISPALIKNPKELVVFTNKIKKDKHHLITSLVPIQL
KRLLSEKNGIDWLKIFDLIWIGGASLSIETSRKCRFEKINLAPCYGSTETAAMVTSLKPKEFLEGLDNVGEILTDIKLRI
SKNGLIQIQSDRIGIELLESYQTKNFKDKNGWWESSDSGEIQKLNNSLYLKFIRRTDNAFNSGGEIVFPEVIKLRLNEFI
LNKKIPIENFIISNIPNKEWGNKMVIIINFKKQTDKTNIEYSLKLLKNFSKNWPKHERPKNWIIGKPKKDDIKNSNYMFT
KL

Sequences:

>Translated_402_residues
MKNKIHIIEVENNEKESTKKILKNIKEKKIIYIKNRSHEYEEIYKAINIRGPAVILNSSGSSGKPRKCIHKLENLYSSAK
ASGIWLERQGFKLKHCLVFNTLPLNHISGLMPLFRSQIWDCEHINISPALIKNPKELVVFTNKIKKDKHHLITSLVPIQL
KRLLSEKNGIDWLKIFDLIWIGGASLSIETSRKCRFEKINLAPCYGSTETAAMVTSLKPKEFLEGLDNVGEILTDIKLRI
SKNGLIQIQSDRIGIELLESYQTKNFKDKNGWWESSDSGEIQKLNNSLYLKFIRRTDNAFNSGGEIVFPEVIKLRLNEFI
LNKKIPIENFIISNIPNKEWGNKMVIIINFKKQTDKTNIEYSLKLLKNFSKNWPKHERPKNWIIGKPKKDDIKNSNYMFT
KL
>Mature_402_residues
MKNKIHIIEVENNEKESTKKILKNIKEKKIIYIKNRSHEYEEIYKAINIRGPAVILNSSGSSGKPRKCIHKLENLYSSAK
ASGIWLERQGFKLKHCLVFNTLPLNHISGLMPLFRSQIWDCEHINISPALIKNPKELVVFTNKIKKDKHHLITSLVPIQL
KRLLSEKNGIDWLKIFDLIWIGGASLSIETSRKCRFEKINLAPCYGSTETAAMVTSLKPKEFLEGLDNVGEILTDIKLRI
SKNGLIQIQSDRIGIELLESYQTKNFKDKNGWWESSDSGEIQKLNNSLYLKFIRRTDNAFNSGGEIVFPEVIKLRLNEFI
LNKKIPIENFIISNIPNKEWGNKMVIIINFKKQTDKTNIEYSLKLLKNFSKNWPKHERPKNWIIGKPKKDDIKNSNYMFT
KL

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=297, Percent_Identity=25.2525252525253, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 46587; Mature: 46587

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKIHIIEVENNEKESTKKILKNIKEKKIIYIKNRSHEYEEIYKAINIRGPAVILNSSG
CCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCC
SSGKPRKCIHKLENLYSSAKASGIWLERQGFKLKHCLVFNTLPLNHISGLMPLFRSQIWD
CCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCC
CEHINISPALIKNPKELVVFTNKIKKDKHHLITSLVPIQLKRLLSEKNGIDWLKIFDLIW
CCCCCCCHHHHCCCHHEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IGGASLSIETSRKCRFEKINLAPCYGSTETAAMVTSLKPKEFLEGLDNVGEILTDIKLRI
CCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEE
SKNGLIQIQSDRIGIELLESYQTKNFKDKNGWWESSDSGEIQKLNNSLYLKFIRRTDNAF
CCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHEEHEEHHCCCCC
NSGGEIVFPEVIKLRLNEFILNKKIPIENFIISNIPNKEWGNKMVIIINFKKQTDKTNIE
CCCCCEECHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHH
YSLKLLKNFSKNWPKHERPKNWIIGKPKKDDIKNSNYMFTKL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKNKIHIIEVENNEKESTKKILKNIKEKKIIYIKNRSHEYEEIYKAINIRGPAVILNSSG
CCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCC
SSGKPRKCIHKLENLYSSAKASGIWLERQGFKLKHCLVFNTLPLNHISGLMPLFRSQIWD
CCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCC
CEHINISPALIKNPKELVVFTNKIKKDKHHLITSLVPIQLKRLLSEKNGIDWLKIFDLIW
CCCCCCCHHHHCCCHHEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IGGASLSIETSRKCRFEKINLAPCYGSTETAAMVTSLKPKEFLEGLDNVGEILTDIKLRI
CCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEE
SKNGLIQIQSDRIGIELLESYQTKNFKDKNGWWESSDSGEIQKLNNSLYLKFIRRTDNAF
CCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHEEHEEHHCCCCC
NSGGEIVFPEVIKLRLNEFILNKKIPIENFIISNIPNKEWGNKMVIIINFKKQTDKTNIE
CCCCCEECHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHH
YSLKLLKNFSKNWPKHERPKNWIIGKPKKDDIKNSNYMFTKL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]