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The map label for this gene is mraW

Identifier: 123965435

GI number: 123965435

Start: 182385

End: 183287

Strand: Reverse

Name: mraW

Synonym: P9515_02001

Alternate gene names: 123965435

Gene position: 183287-182385 (Counterclockwise)

Preceding gene: 123965438

Following gene: 123965433

Centisome position: 10.76

GC content: 30.01

Gene sequence:

>903_bases
ATGCAAACTGACCTTTCTAATTCATCTTTGTTCAATCATAAGTCAGTGATGACAGATGAAATTTTATATTCTATAGATCA
ATATCCATTTATATCAGACAATAAACTAACAGCTATAGATGCGACTTTAGGCGGTGGTGGGCACTCATATCAATTATTAA
AAAAATATCCTGATTTAAAAATAATTGGATTAGACCACGATCCAATCGCAAGGGAATCAGCACTTAATAAACTTGAAGAA
TTCAAATCGAGGATTGAAATAATTCCTTCAAATTTTTCTAACTTTGAGCCAAAAGAAAAAGTTTCTTTTGTGATAGCAGA
TCTTGGAGTAAATAGTAATCAAATAGATAGCCCGGAAAGAGGATTTAGTTTTCAAAAAGATGGTCCTTTAGATATGCGAA
TGAATCCTTTAATTAAGATGAATGCGGAGAACTTAATTGAGACTTTAAGTGAAAAAGATCTGGCTGATTTAATTTTTAAA
TTTGGTGATGAAAGATTATCACGAAAGATTTCGAGGAAAATTAAAAAAGATTTAAAAGAAAAAGGTAAATATTCAGGAAC
AAAAGATTTAGCATATTCAATTGCTGGTTGTTTCCCGCCCAAACAAAGATATCGAAAAATTCATCCTGCAACTAGAACAT
TCCAAGCACTTAGAATAGCTGTAAATAACGAGATTGAAGCATTAGAAAAATTTCTAAAGATTGCTCCTGATTGGCTATTG
ACTGGGGGAATAATTTCAATTATTAGCTTTCATTCAATAGAGGATCGGTTGGTGAAAAATTCTTTTAAAGGTGATTACAG
ATTAAAAAATCTTACAAAAAAACCAATTACACCTAATAAAAAAGAAATTGAAAATAATAAAAGATCTCGAAGTGCAAAAT
TAAGAATTGCTCAATTGAAATAA

Upstream 100 bases:

>100_bases
GATGGATGATGAGGGCCAAAGTTGACCACCATCGGTTCTGTTCTAGTCTCTAGTTGAGCCATTAATAATTTAACCTCTAT
TTAAATCTTAGTAGAAAGTT

Downstream 100 bases:

>100_bases
TTAAGCCCTATCGCAATAAATTAATAGTATCTGTAATTATCTGAATTGAATTTTCTATATCTTCTGAAGTAGTCATCAAT
CCAATACTTAATCTTAATGA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MQTDLSNSSLFNHKSVMTDEILYSIDQYPFISDNKLTAIDATLGGGGHSYQLLKKYPDLKIIGLDHDPIARESALNKLEE
FKSRIEIIPSNFSNFEPKEKVSFVIADLGVNSNQIDSPERGFSFQKDGPLDMRMNPLIKMNAENLIETLSEKDLADLIFK
FGDERLSRKISRKIKKDLKEKGKYSGTKDLAYSIAGCFPPKQRYRKIHPATRTFQALRIAVNNEIEALEKFLKIAPDWLL
TGGIISIISFHSIEDRLVKNSFKGDYRLKNLTKKPITPNKKEIENNKRSRSAKLRIAQLK

Sequences:

>Translated_300_residues
MQTDLSNSSLFNHKSVMTDEILYSIDQYPFISDNKLTAIDATLGGGGHSYQLLKKYPDLKIIGLDHDPIARESALNKLEE
FKSRIEIIPSNFSNFEPKEKVSFVIADLGVNSNQIDSPERGFSFQKDGPLDMRMNPLIKMNAENLIETLSEKDLADLIFK
FGDERLSRKISRKIKKDLKEKGKYSGTKDLAYSIAGCFPPKQRYRKIHPATRTFQALRIAVNNEIEALEKFLKIAPDWLL
TGGIISIISFHSIEDRLVKNSFKGDYRLKNLTKKPITPNKKEIENNKRSRSAKLRIAQLK
>Mature_300_residues
MQTDLSNSSLFNHKSVMTDEILYSIDQYPFISDNKLTAIDATLGGGGHSYQLLKKYPDLKIIGLDHDPIARESALNKLEE
FKSRIEIIPSNFSNFEPKEKVSFVIADLGVNSNQIDSPERGFSFQKDGPLDMRMNPLIKMNAENLIETLSEKDLADLIFK
FGDERLSRKISRKIKKDLKEKGKYSGTKDLAYSIAGCFPPKQRYRKIHPATRTFQALRIAVNNEIEALEKFLKIAPDWLL
TGGIISIISFHSIEDRLVKNSFKGDYRLKNLTKKPITPNKKEIENNKRSRSAKLRIAQLK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=342, Percent_Identity=33.6257309941521, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI165377202, Length=174, Percent_Identity=34.4827586206897, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=35.8490566037736, Blast_Score=173, Evalue=1e-44,
Organism=Drosophila melanogaster, GI62472493, Length=307, Percent_Identity=33.8762214983713, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI28571637, Length=307, Percent_Identity=33.8762214983713, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PROM5 (A2BUE8)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010516.1
- ProteinModelPortal:   A2BUE8
- SMR:   A2BUE8
- STRING:   A2BUE8
- GeneID:   4720336
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_02001
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   PMAR167542:P9515ORF_0207-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34208; Mature: 34208

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS01048 RIBOSOMAL_S6

Important sites: BINDING 65-65 BINDING 92-92 BINDING 107-107 BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTDLSNSSLFNHKSVMTDEILYSIDQYPFISDNKLTAIDATLGGGGHSYQLLKKYPDLK
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCEE
IIGLDHDPIARESALNKLEEFKSRIEIIPSNFSNFEPKEKVSFVIADLGVNSNQIDSPER
EEECCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHEEEEEECCCCCCCCCCCHH
GFSFQKDGPLDMRMNPLIKMNAENLIETLSEKDLADLIFKFGDERLSRKISRKIKKDLKE
CCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
KGKYSGTKDLAYSIAGCFPPKQRYRKIHPATRTFQALRIAVNNEIEALEKFLKIAPDWLL
HCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHH
TGGIISIISFHSIEDRLVKNSFKGDYRLKNLTKKPITPNKKEIENNKRSRSAKLRIAQLK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MQTDLSNSSLFNHKSVMTDEILYSIDQYPFISDNKLTAIDATLGGGGHSYQLLKKYPDLK
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCEE
IIGLDHDPIARESALNKLEEFKSRIEIIPSNFSNFEPKEKVSFVIADLGVNSNQIDSPER
EEECCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHEEEEEECCCCCCCCCCCHH
GFSFQKDGPLDMRMNPLIKMNAENLIETLSEKDLADLIFKFGDERLSRKISRKIKKDLKE
CCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
KGKYSGTKDLAYSIAGCFPPKQRYRKIHPATRTFQALRIAVNNEIEALEKFLKIAPDWLL
HCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHH
TGGIISIISFHSIEDRLVKNSFKGDYRLKNLTKKPITPNKKEIENNKRSRSAKLRIAQLK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA