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The map label for this gene is plsB [H]

Identifier: 120596969

GI number: 120596969

Start: 163225

End: 165648

Strand: Direct

Name: plsB [H]

Synonym: Sputw3181_0136

Alternate gene names: 120596969

Gene position: 163225-165648 (Clockwise)

Preceding gene: 120596957

Following gene: 120596970

Centisome position: 3.47

GC content: 43.56

Gene sequence:

>2424_bases
ATGCCTAAACAAGACTCTCTTTGGTTGAAATCATTACGTTGGATCCAAAAACACTTAGTCCACACTATTGTGGTGCCTCA
GGATCCTTTTGCCGATCTGAATCTGGATACCTCCAGACCACTTGCTTATGTCATGAAAACAGAATCGCTGAGTGACATTG
CGGCACTAAGTGAAGTCACCGAAAAATTAGGCTTGCCAAGCCCCTACGAACCTTTAGTTGTTAACGGTGTTGTTGCGCCA
CGGGTTGTTTGCCTTCAAGGACGTAAACCTTTGTTTGGTGAACGTGCGGGTAACGAGCCATTCCTTGAGTGTTTTATGCG
TCTCTTGGCTGTTCATAAGGAGCGCCCGGAGCTTGATATTCAGTTAGTGCCAGTGAGTCTTTATTGGGGACGTACTCCAG
GTAAAGAAGACGATACGATGAAAGCGGCTGTGTTGGAGCGTGAAAATCCAACTTGGCTACGTAAGTGCTTCATGATTTTG
TTTCTAGGCCGTCATAATTTTGTACAGTTTTCGAATGCAGTTTCACTGCGTTATATGGCAGATGAACACGGTACTGATAT
GGGGATAGCCCATAAATTAGCGCGTGTGGCACGGGTGCATTTTCGCCGCCAACGTAAGGTGATGACGGGTCCTTTACTGC
CAAATCGTCAGGCATTATTTGATTCGTTGCTCAAATCGGAGTCATTACGTAAGGCGATTCAAGAAGAGGCTGTGAGCAAA
AAGATTTCTGAAACTCAATCTCGTGAAACTGCAATTGAGTATCTTGATGAAATAGCAGCAAATTATTCAGATAGTTTAGT
GCGCATTGCCGAGCGTTTTTTAACCTGGTTATGGAATAAGCTCTACAGTGGCATCAATATTAAAGGTGCCGAACATATTC
GTCAGTTACACCATGATGGTCACGAAATTGTCTATGTGCCTTGTCATCGAAGTCATATGGATTATCTATTGCTATCCTAC
ATTTTGTATTACCAAGGTATGGTGCCGCCGCATATTGCAGCAGGGATAAACCTTAACTTTTGGCCCGCGGGACCATTGTT
CCGCCGTGGTGGCGCGTTCTTTATCCGTCGTAGTTTTAATGGTAATAAGCTTTATACCGCAGTCTTTCGTGAATATTTAG
ATCAACTGTTTGCCAAGGGCTATTCGGTGGAGTATTTCTCTGAGGGCGGTCGGTCTCGTACGGGGCGTTTGTTAGCGCCC
AAAACGGGCATGATAGCAATGACAATGAACAGCGTGCTGCGCGGAATTGAGCGCCCAGTTACTTTGGTTCCCGTATATTT
AGGTTATGACCATGTGATGGAAGTCGCCACGTATCACAAAGAGTTAAGTGGCAAGAAAAAACAAAAAGAATCCGTTTGGC
AAGTTTTTGGAGCTATTCGCAAGTTAGGTAATTTTGGTCAAGGTTATGTGAACTTTGGAGAGCCGATTACCCTGCAAACC
TTTTTAAACGAAATGGCACCTAACTGGCGCGCAGAGCTTGCCGATGATCCTGAACAAAAGCCAACTTGGTTAACTCCAGC
GGTTAACGTGTTAGCAAATCGGGTGATGACACGTATTAATGATGCGGCAGCGGCCAGTTCCGTCACGCTCACCAGTTTAG
CTTTATTGGCTTCTGAGCAAAATGCGCTTGAGCGTTGTTTGTTAGAACGTCAGTTAGATTTGTACCTCACGCTGCTGAAG
CGGGTGCCTTATACCTCTTTTACTTCTGTCGCGGAAGGTGATGGTAAGCATCTTGTTCAGCAGGGTATTGATTTAAATAA
GTTTTCAGTATCCAGTGATCCTTTAGGTGAGATAGTGTCTATCGATGCTAATCAGGCCATTTCGATGACCTATTATCGCA
ATAATATTATCCACTTATTTATTATTCCTTCTCTTATTGCGAGTTGTTTGACCCATAATGAGCAAAGTCAGCGACAGCAA
ATTGTGGCAATCGTGAATGACTTTTATCCGTTGCTAAAGGCTGAGCTGTTTATGGGAATTAAGGATATACCTTCCTATGT
CAATCAAGTGCTGGATCTCTTTATTGAGCAAGGCTTAATAACTGAGTCAGATAACTTATCAGTTGTAACGGAACATTCCA
GTCAGTTGGTATTGCTCTCAGGATCGGTCAGTGAGACATTGCAACGCTATGCCATCATTTTTAATTTGCTAGCACATCGT
CCTAAGATGGAACGTTCAGAACTCGAGTCTGAAAGCCATTTACTTGCTCAGCGTTTAGGGGCATTGCATGGCATTACAGC
ACCAGAGTTTTACGATAAAAAACTCTACGGCACTTTGAGTGTTAAGTTAAAAGAGTTAGGTTATCTTTCTGATAAAGATG
ATAAATCTGATGTTAAGCGTATTCGAGATCAAGCCAATAGCCTATTGAGAGCCTCAGTGAGACAGACGATAGTTGCGAGT
GTCACTGCGGAGCATATTAGTTAA

Upstream 100 bases:

>100_bases
TGTTAGGGCGATTTAGGGATATATAGGAGAGAATTAGCGAATACTCCCCAGAGTCTGGTATTCTATTGGGCTAGTAAACG
TACTTAATTGCATAAGAATA

Downstream 100 bases:

>100_bases
TGACAAATCATATGAATGCGGTTAAGCATAAAACCGCAGGTAAGTCTTTGCTCGGTGGCGCCATGATTATTGCTGGGACG
ACCGTAGGGGCGGGCATGTT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MPKQDSLWLKSLRWIQKHLVHTIVVPQDPFADLNLDTSRPLAYVMKTESLSDIAALSEVTEKLGLPSPYEPLVVNGVVAP
RVVCLQGRKPLFGERAGNEPFLECFMRLLAVHKERPELDIQLVPVSLYWGRTPGKEDDTMKAAVLERENPTWLRKCFMIL
FLGRHNFVQFSNAVSLRYMADEHGTDMGIAHKLARVARVHFRRQRKVMTGPLLPNRQALFDSLLKSESLRKAIQEEAVSK
KISETQSRETAIEYLDEIAANYSDSLVRIAERFLTWLWNKLYSGINIKGAEHIRQLHHDGHEIVYVPCHRSHMDYLLLSY
ILYYQGMVPPHIAAGINLNFWPAGPLFRRGGAFFIRRSFNGNKLYTAVFREYLDQLFAKGYSVEYFSEGGRSRTGRLLAP
KTGMIAMTMNSVLRGIERPVTLVPVYLGYDHVMEVATYHKELSGKKKQKESVWQVFGAIRKLGNFGQGYVNFGEPITLQT
FLNEMAPNWRAELADDPEQKPTWLTPAVNVLANRVMTRINDAAAASSVTLTSLALLASEQNALERCLLERQLDLYLTLLK
RVPYTSFTSVAEGDGKHLVQQGIDLNKFSVSSDPLGEIVSIDANQAISMTYYRNNIIHLFIIPSLIASCLTHNEQSQRQQ
IVAIVNDFYPLLKAELFMGIKDIPSYVNQVLDLFIEQGLITESDNLSVVTEHSSQLVLLSGSVSETLQRYAIIFNLLAHR
PKMERSELESESHLLAQRLGALHGITAPEFYDKKLYGTLSVKLKELGYLSDKDDKSDVKRIRDQANSLLRASVRQTIVAS
VTAEHIS

Sequences:

>Translated_807_residues
MPKQDSLWLKSLRWIQKHLVHTIVVPQDPFADLNLDTSRPLAYVMKTESLSDIAALSEVTEKLGLPSPYEPLVVNGVVAP
RVVCLQGRKPLFGERAGNEPFLECFMRLLAVHKERPELDIQLVPVSLYWGRTPGKEDDTMKAAVLERENPTWLRKCFMIL
FLGRHNFVQFSNAVSLRYMADEHGTDMGIAHKLARVARVHFRRQRKVMTGPLLPNRQALFDSLLKSESLRKAIQEEAVSK
KISETQSRETAIEYLDEIAANYSDSLVRIAERFLTWLWNKLYSGINIKGAEHIRQLHHDGHEIVYVPCHRSHMDYLLLSY
ILYYQGMVPPHIAAGINLNFWPAGPLFRRGGAFFIRRSFNGNKLYTAVFREYLDQLFAKGYSVEYFSEGGRSRTGRLLAP
KTGMIAMTMNSVLRGIERPVTLVPVYLGYDHVMEVATYHKELSGKKKQKESVWQVFGAIRKLGNFGQGYVNFGEPITLQT
FLNEMAPNWRAELADDPEQKPTWLTPAVNVLANRVMTRINDAAAASSVTLTSLALLASEQNALERCLLERQLDLYLTLLK
RVPYTSFTSVAEGDGKHLVQQGIDLNKFSVSSDPLGEIVSIDANQAISMTYYRNNIIHLFIIPSLIASCLTHNEQSQRQQ
IVAIVNDFYPLLKAELFMGIKDIPSYVNQVLDLFIEQGLITESDNLSVVTEHSSQLVLLSGSVSETLQRYAIIFNLLAHR
PKMERSELESESHLLAQRLGALHGITAPEFYDKKLYGTLSVKLKELGYLSDKDDKSDVKRIRDQANSLLRASVRQTIVAS
VTAEHIS
>Mature_806_residues
PKQDSLWLKSLRWIQKHLVHTIVVPQDPFADLNLDTSRPLAYVMKTESLSDIAALSEVTEKLGLPSPYEPLVVNGVVAPR
VVCLQGRKPLFGERAGNEPFLECFMRLLAVHKERPELDIQLVPVSLYWGRTPGKEDDTMKAAVLERENPTWLRKCFMILF
LGRHNFVQFSNAVSLRYMADEHGTDMGIAHKLARVARVHFRRQRKVMTGPLLPNRQALFDSLLKSESLRKAIQEEAVSKK
ISETQSRETAIEYLDEIAANYSDSLVRIAERFLTWLWNKLYSGINIKGAEHIRQLHHDGHEIVYVPCHRSHMDYLLLSYI
LYYQGMVPPHIAAGINLNFWPAGPLFRRGGAFFIRRSFNGNKLYTAVFREYLDQLFAKGYSVEYFSEGGRSRTGRLLAPK
TGMIAMTMNSVLRGIERPVTLVPVYLGYDHVMEVATYHKELSGKKKQKESVWQVFGAIRKLGNFGQGYVNFGEPITLQTF
LNEMAPNWRAELADDPEQKPTWLTPAVNVLANRVMTRINDAAAASSVTLTSLALLASEQNALERCLLERQLDLYLTLLKR
VPYTSFTSVAEGDGKHLVQQGIDLNKFSVSSDPLGEIVSIDANQAISMTYYRNNIIHLFIIPSLIASCLTHNEQSQRQQI
VAIVNDFYPLLKAELFMGIKDIPSYVNQVLDLFIEQGLITESDNLSVVTEHSSQLVLLSGSVSETLQRYAIIFNLLAHRP
KMERSELESESHLLAQRLGALHGITAPEFYDKKLYGTLSVKLKELGYLSDKDDKSDVKRIRDQANSLLRASVRQTIVASV
TAEHIS

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=264, Percent_Identity=35.9848484848485, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI190358539, Length=550, Percent_Identity=24.3636363636364, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI87082362, Length=791, Percent_Identity=49.3046776232617, Blast_Score=782, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71988723, Length=452, Percent_Identity=28.0973451327434, Blast_Score=152, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI25147672, Length=305, Percent_Identity=35.0819672131148, Blast_Score=150, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71988728, Length=393, Percent_Identity=30.0254452926209, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17864692, Length=325, Percent_Identity=31.6923076923077, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24650754, Length=485, Percent_Identity=27.2164948453608, Blast_Score=138, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21357731, Length=484, Percent_Identity=27.2727272727273, Blast_Score=138, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24650752, Length=485, Percent_Identity=27.2164948453608, Blast_Score=138, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 91377; Mature: 91245

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQDSLWLKSLRWIQKHLVHTIVVPQDPFADLNLDTSRPLAYVMKTESLSDIAALSEVT
CCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
EKLGLPSPYEPLVVNGVVAPRVVCLQGRKPLFGERAGNEPFLECFMRLLAVHKERPELDI
HHCCCCCCCCCEEECCCHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEE
QLVPVSLYWGRTPGKEDDTMKAAVLERENPTWLRKCFMILFLGRHNFVQFSNAVSLRYMA
EEEEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEC
DEHGTDMGIAHKLARVARVHFRRQRKVMTGPLLPNRQALFDSLLKSESLRKAIQEEAVSK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KISETQSRETAIEYLDEIAANYSDSLVRIAERFLTWLWNKLYSGINIKGAEHIRQLHHDG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
HEIVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPLFRRGGAFFIRRSFN
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCHHHHCCCEEEEEECCC
GNKLYTAVFREYLDQLFAKGYSVEYFSEGGRSRTGRLLAPKTGMIAMTMNSVLRGIERPV
CCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCE
TLVPVYLGYDHVMEVATYHKELSGKKKQKESVWQVFGAIRKLGNFGQGYVNFGEPITLQT
EEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHH
FLNEMAPNWRAELADDPEQKPTWLTPAVNVLANRVMTRINDAAAASSVTLTSLALLASEQ
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALERCLLERQLDLYLTLLKRVPYTSFTSVAEGDGKHLVQQGIDLNKFSVSSDPLGEIVS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHCCCCCCEEECCCCCCCCEEE
IDANQAISMTYYRNNIIHLFIIPSLIASCLTHNEQSQRQQIVAIVNDFYPLLKAELFMGI
ECCCCEEEEEEEECCEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCH
KDIPSYVNQVLDLFIEQGLITESDNLSVVTEHSSQLVLLSGSVSETLQRYAIIFNLLAHR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCC
PKMERSELESESHLLAQRLGALHGITAPEFYDKKLYGTLSVKLKELGYLSDKDDKSDVKR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEHHHHCCCCCCCCCHHHHHH
IRDQANSLLRASVRQTIVASVTAEHIS
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKQDSLWLKSLRWIQKHLVHTIVVPQDPFADLNLDTSRPLAYVMKTESLSDIAALSEVT
CCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
EKLGLPSPYEPLVVNGVVAPRVVCLQGRKPLFGERAGNEPFLECFMRLLAVHKERPELDI
HHCCCCCCCCCEEECCCHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEE
QLVPVSLYWGRTPGKEDDTMKAAVLERENPTWLRKCFMILFLGRHNFVQFSNAVSLRYMA
EEEEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEC
DEHGTDMGIAHKLARVARVHFRRQRKVMTGPLLPNRQALFDSLLKSESLRKAIQEEAVSK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KISETQSRETAIEYLDEIAANYSDSLVRIAERFLTWLWNKLYSGINIKGAEHIRQLHHDG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
HEIVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPLFRRGGAFFIRRSFN
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCHHHHCCCEEEEEECCC
GNKLYTAVFREYLDQLFAKGYSVEYFSEGGRSRTGRLLAPKTGMIAMTMNSVLRGIERPV
CCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCE
TLVPVYLGYDHVMEVATYHKELSGKKKQKESVWQVFGAIRKLGNFGQGYVNFGEPITLQT
EEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHH
FLNEMAPNWRAELADDPEQKPTWLTPAVNVLANRVMTRINDAAAASSVTLTSLALLASEQ
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALERCLLERQLDLYLTLLKRVPYTSFTSVAEGDGKHLVQQGIDLNKFSVSSDPLGEIVS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHCCCCCCEEECCCCCCCCEEE
IDANQAISMTYYRNNIIHLFIIPSLIASCLTHNEQSQRQQIVAIVNDFYPLLKAELFMGI
ECCCCEEEEEEEECCEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCH
KDIPSYVNQVLDLFIEQGLITESDNLSVVTEHSSQLVLLSGSVSETLQRYAIIFNLLAHR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCC
PKMERSELESESHLLAQRLGALHGITAPEFYDKKLYGTLSVKLKELGYLSDKDDKSDVKR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEHHHHCCCCCCCCCHHHHHH
IRDQANSLLRASVRQTIVASVTAEHIS
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]