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The map label for this gene is menH [H]

Identifier: 119944135

GI number: 119944135

Start: 453637

End: 454434

Strand: Reverse

Name: menH [H]

Synonym: Ping_0351

Alternate gene names: 119944135

Gene position: 454434-453637 (Counterclockwise)

Preceding gene: 119944136

Following gene: 119944134

Centisome position: 9.97

GC content: 38.6

Gene sequence:

>798_bases
ATGCCACTTTTTAGCCAAACGCAGGGGTCGCCTCAATCTCCTGCTTTAGTTTTTTTGCACGGTTTTTTAGGTAGTCATCA
GGATTGGTGTGAAACTATCAAGCAGCTGAAAAAATCTTTTTATTGTATCTCTATCGATCTGCCCGGGCATGGAGATTCCG
CCTCAGTAACAATACCTAAAGATAAAGGCTTTGAGACAACACATCGGTTGATTAAAAATGTATTAAACGACTTAAAAATC
AAAGAATATATTCTTATTGGTTATTCCTTAGGTGGCCGTATTGCGCTTGATTACGCACGCACACAACAGGATCAGCACTT
AAAAGCCTTGGTCTTAGAATCTTGCCATACCGGTTATCAAACTCAGGATGAAAAAGATAAACGTTTTGCACATGATCTTA
GTTGGGCGAAACGCTTTGCCACACAAAGTATTAGACAAAGTTTAAATCAATGGTATGAACAGGATATTTTCAACGATCTT
TCCTGCGAACAAAAAAATAATCTGTTTAATAAGCGCAGTCGTAATTACGGTGTCTGTTTAGCAAATATGCTGCTGGCAAC
CAGTCTCGCTGAGCAAACCGATGCATTGCCTTTTTTACAAAAAAATACTGCACAAGCAAAACCCTTATCCATTTATTACT
GTTTTGGCGAAAAAGATAATAAATTTAAAACCCTAGGCCGCGCATTAGCCAAGCTTAAAAATATCCAACCAACTGAATTT
GCCGGGGCAGGTCATAATATTCACCAACAGAGTCCTGTGCAATATGCGCGCTTTATTAAGCAGCATTTTAGTCAATAA

Upstream 100 bases:

>100_bases
TATCAAAAATCATTACAGAATCGTCTTTCTTTAATTGAGATCTGTGTCAAAAACGACCAAACTTACAACCATCTTGAATA
CATCAAAGAGCAGATAAAGT

Downstream 100 bases:

>100_bases
TTTTTAGATAATAATTAAGAGAAAAATATGCCTGAAAAAGAGGGTTATGCACCCATTGCATGGAAAAAAAGCGCGTTAAA
CTTCACTGACATTCTCTACC

Product: alpha/beta hydrolase fold

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPLFSQTQGSPQSPALVFLHGFLGSHQDWCETIKQLKKSFYCISIDLPGHGDSASVTIPKDKGFETTHRLIKNVLNDLKI
KEYILIGYSLGGRIALDYARTQQDQHLKALVLESCHTGYQTQDEKDKRFAHDLSWAKRFATQSIRQSLNQWYEQDIFNDL
SCEQKNNLFNKRSRNYGVCLANMLLATSLAEQTDALPFLQKNTAQAKPLSIYYCFGEKDNKFKTLGRALAKLKNIQPTEF
AGAGHNIHQQSPVQYARFIKQHFSQ

Sequences:

>Translated_265_residues
MPLFSQTQGSPQSPALVFLHGFLGSHQDWCETIKQLKKSFYCISIDLPGHGDSASVTIPKDKGFETTHRLIKNVLNDLKI
KEYILIGYSLGGRIALDYARTQQDQHLKALVLESCHTGYQTQDEKDKRFAHDLSWAKRFATQSIRQSLNQWYEQDIFNDL
SCEQKNNLFNKRSRNYGVCLANMLLATSLAEQTDALPFLQKNTAQAKPLSIYYCFGEKDNKFKTLGRALAKLKNIQPTEF
AGAGHNIHQQSPVQYARFIKQHFSQ
>Mature_264_residues
PLFSQTQGSPQSPALVFLHGFLGSHQDWCETIKQLKKSFYCISIDLPGHGDSASVTIPKDKGFETTHRLIKNVLNDLKIK
EYILIGYSLGGRIALDYARTQQDQHLKALVLESCHTGYQTQDEKDKRFAHDLSWAKRFATQSIRQSLNQWYEQDIFNDLS
CEQKNNLFNKRSRNYGVCLANMLLATSLAEQTDALPFLQKNTAQAKPLSIYYCFGEKDNKFKTLGRALAKLKNIQPTEFA
GAGHNIHQQSPVQYARFIKQHFSQ

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=253, Percent_Identity=36.7588932806324, Blast_Score=157, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 30236; Mature: 30104

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLFSQTQGSPQSPALVFLHGFLGSHQDWCETIKQLKKSFYCISIDLPGHGDSASVTIPK
CCCCCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCEEECCC
DKGFETTHRLIKNVLNDLKIKEYILIGYSLGGRIALDYARTQQDQHLKALVLESCHTGYQ
CCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEHHHHCCHHHHHHHHHHHHHHCCCC
TQDEKDKRFAHDLSWAKRFATQSIRQSLNQWYEQDIFNDLSCEQKNNLFNKRSRNYGVCL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHH
ANMLLATSLAEQTDALPFLQKNTAQAKPLSIYYCFGEKDNKFKTLGRALAKLKNIQPTEF
HHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
AGAGHNIHQQSPVQYARFIKQHFSQ
CCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLFSQTQGSPQSPALVFLHGFLGSHQDWCETIKQLKKSFYCISIDLPGHGDSASVTIPK
CCCCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCEEECCC
DKGFETTHRLIKNVLNDLKIKEYILIGYSLGGRIALDYARTQQDQHLKALVLESCHTGYQ
CCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEHHHHCCHHHHHHHHHHHHHHCCCC
TQDEKDKRFAHDLSWAKRFATQSIRQSLNQWYEQDIFNDLSCEQKNNLFNKRSRNYGVCL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHH
ANMLLATSLAEQTDALPFLQKNTAQAKPLSIYYCFGEKDNKFKTLGRALAKLKNIQPTEF
HHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
AGAGHNIHQQSPVQYARFIKQHFSQ
CCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA