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The map label for this gene is menD

Identifier: 119944136

GI number: 119944136

Start: 454421

End: 456166

Strand: Reverse

Name: menD

Synonym: Ping_0352

Alternate gene names: 119944136

Gene position: 456166-454421 (Counterclockwise)

Preceding gene: 119944137

Following gene: 119944135

Centisome position: 10.0

GC content: 41.35

Gene sequence:

>1746_bases
ATGATCACTACGTTATATAAAGAGCCTGCTTCATTTGCTCATGAGTTTTCTAATATCAACCTGTTGTGGGCATCACTGTT
TATTGAAGAGCTGGTGCGCAACGGTATTTCAGATTTTTGTATTGCCCCCGGATCCCGTTCAACCCCTTTAACATTAGCGG
CTGACCAGCATACAGAGGCGCATACCCATGTCCATTTTGATGAACGGGGGTTAGGTTTTTTAGCACTTGGTCTGAGTCTC
TTTAGCCATAAACCCGTGGTTATTATTACCACCTCCGGCACCGCGGTCGCAAACCTTTATCCTGCCGTTATTGAAGCCAA
ACTAAGCGCGATTCCCTTAATAATTTTATCTGCTGACAGACCCGTTGAATTAATCGATTGTGGCGCGAATCAAGCCATTG
ACCAATATCGAATCTTTTCCCATTATCCGGTTTTTTTTGCACAAATACCCAGTGCTACGACACACATTAAACCTAATTAC
CTGTTAACCACTATCAATCAAGGGTTACAGCAGCAGCGGCAGACCCCCGCAGCGATTCATTTTAATATCGCGTTTTCAGA
GCCGTTATATCCGCAAACAGCAACGCTTAATTATCAAGGCTATTTACAATCATTAAAACAGTGGCTAATAGATAAACAAC
CCTTTAGCCAATATTTTCAGAATAAAGACTCTTTTCAAGCGGCAAGCAATACTCAGCTTAGGGATAAAAAGGTGCTGGTT
ATTGCCGGTCGCGTCACTGGTATCAATCAAGCTCAAGCCATTGCTGAATTTGCTGCATTGAATAATTACCCACTTTTAGC
CGATCTGCAATCCGCATTAACAGGCAATGCAAATAACCTGCACTATTATGACCTGTTATTGGTCAATAAACAGTTTACTG
AAAACCTGCAGCAGGCCGATATTATTGTACAGTTTGGGGGTAAGCTTATTTCCAAACGCCTTAGTCAATTTATTGAAGGA
TTTGCAGGCGAGTATTTGTTGGTCGATCCAGGTAATACTAGAATCGATCCTGCTCACAGCTTAAGAAAAAGATTTGTATG
CTCAGCCACGCAATGGATAAAAAGTCTACAGAATAAAATACCAGATATTGATAAACACTGGTCACAGGTACTGCAACAGC
AAAACAATTATATAACGAAGCAGATAATAAGCCCTTTTTTGGACAATAATCTCATTAGTGAAATATCTGTCATCTCTGCA
CTGGATAAGTTGCTGCCTGCTGACAACCCGGTATTTATCGGTAACAGTATGCCGGTTCGTTTGAGTGATATGTTTTTCAG
ACAAAACGCAGCATTGCCCTTTAGCAATCGAGGGGCAAGCGGCATTGATGGTTTGCTGGCAACGGCAAGCGGCATTGCAA
AGTCTTGCGCGAGTATTACCACACTCTTAATTGGCGACACCTCTTTTCTTCACGATTTAAATTCCCTGGCTTTATTAAAA
CAACTGCAAGGGCCCTTTGTGATCATTGTGTTTAACAATGATGGCGGCGCGATATTTAATCTTCTGCCGGTGCCTACCCA
GCAAAAACAAGATTATTACCAATTACCCCATGGTTTAACCTTTGCCGACAGCTGCAGGCAATTTTCAATCGATTATTACC
AACCCGAAAGTCTGGATCAATTTGTCGGTGATTATCAAAAATCATTACAGAATCGTCTTTCTTTAATTGAGATCTGTGTC
AAAAACGACCAAACTTACAACCATCTTGAATACATCAAAGAGCAGATAAAGTATGCCACTTTTTAG

Upstream 100 bases:

>100_bases
GCATTGTGGTAGGTTCAATTGCAGATCAAGAATGGCAGGAATTGGAAAATAAAATTCACATTATTTTAGATATTTTATCG
GTGTAATTTAAGGTAAAAAA

Downstream 100 bases:

>100_bases
CCAAACGCAGGGGTCGCCTCAATCTCCTGCTTTAGTTTTTTTGCACGGTTTTTTAGGTAGTCATCAGGATTGGTGTGAAA
CTATCAAGCAGCTGAAAAAA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MITTLYKEPASFAHEFSNINLLWASLFIEELVRNGISDFCIAPGSRSTPLTLAADQHTEAHTHVHFDERGLGFLALGLSL
FSHKPVVIITTSGTAVANLYPAVIEAKLSAIPLIILSADRPVELIDCGANQAIDQYRIFSHYPVFFAQIPSATTHIKPNY
LLTTINQGLQQQRQTPAAIHFNIAFSEPLYPQTATLNYQGYLQSLKQWLIDKQPFSQYFQNKDSFQAASNTQLRDKKVLV
IAGRVTGINQAQAIAEFAALNNYPLLADLQSALTGNANNLHYYDLLLVNKQFTENLQQADIIVQFGGKLISKRLSQFIEG
FAGEYLLVDPGNTRIDPAHSLRKRFVCSATQWIKSLQNKIPDIDKHWSQVLQQQNNYITKQIISPFLDNNLISEISVISA
LDKLLPADNPVFIGNSMPVRLSDMFFRQNAALPFSNRGASGIDGLLATASGIAKSCASITTLLIGDTSFLHDLNSLALLK
QLQGPFVIIVFNNDGGAIFNLLPVPTQQKQDYYQLPHGLTFADSCRQFSIDYYQPESLDQFVGDYQKSLQNRLSLIEICV
KNDQTYNHLEYIKEQIKYATF

Sequences:

>Translated_581_residues
MITTLYKEPASFAHEFSNINLLWASLFIEELVRNGISDFCIAPGSRSTPLTLAADQHTEAHTHVHFDERGLGFLALGLSL
FSHKPVVIITTSGTAVANLYPAVIEAKLSAIPLIILSADRPVELIDCGANQAIDQYRIFSHYPVFFAQIPSATTHIKPNY
LLTTINQGLQQQRQTPAAIHFNIAFSEPLYPQTATLNYQGYLQSLKQWLIDKQPFSQYFQNKDSFQAASNTQLRDKKVLV
IAGRVTGINQAQAIAEFAALNNYPLLADLQSALTGNANNLHYYDLLLVNKQFTENLQQADIIVQFGGKLISKRLSQFIEG
FAGEYLLVDPGNTRIDPAHSLRKRFVCSATQWIKSLQNKIPDIDKHWSQVLQQQNNYITKQIISPFLDNNLISEISVISA
LDKLLPADNPVFIGNSMPVRLSDMFFRQNAALPFSNRGASGIDGLLATASGIAKSCASITTLLIGDTSFLHDLNSLALLK
QLQGPFVIIVFNNDGGAIFNLLPVPTQQKQDYYQLPHGLTFADSCRQFSIDYYQPESLDQFVGDYQKSLQNRLSLIEICV
KNDQTYNHLEYIKEQIKYATF
>Mature_581_residues
MITTLYKEPASFAHEFSNINLLWASLFIEELVRNGISDFCIAPGSRSTPLTLAADQHTEAHTHVHFDERGLGFLALGLSL
FSHKPVVIITTSGTAVANLYPAVIEAKLSAIPLIILSADRPVELIDCGANQAIDQYRIFSHYPVFFAQIPSATTHIKPNY
LLTTINQGLQQQRQTPAAIHFNIAFSEPLYPQTATLNYQGYLQSLKQWLIDKQPFSQYFQNKDSFQAASNTQLRDKKVLV
IAGRVTGINQAQAIAEFAALNNYPLLADLQSALTGNANNLHYYDLLLVNKQFTENLQQADIIVQFGGKLISKRLSQFIEG
FAGEYLLVDPGNTRIDPAHSLRKRFVCSATQWIKSLQNKIPDIDKHWSQVLQQQNNYITKQIISPFLDNNLISEISVISA
LDKLLPADNPVFIGNSMPVRLSDMFFRQNAALPFSNRGASGIDGLLATASGIAKSCASITTLLIGDTSFLHDLNSLALLK
QLQGPFVIIVFNNDGGAIFNLLPVPTQQKQDYYQLPHGLTFADSCRQFSIDYYQPESLDQFVGDYQKSLQNRLSLIEICV
KNDQTYNHLEYIKEQIKYATF

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=569, Percent_Identity=37.0826010544815, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1790104, Length=305, Percent_Identity=24.5901639344262, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_PSYIN (A1SRV2)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941816.1
- ProteinModelPortal:   A1SRV2
- STRING:   A1SRV2
- GeneID:   4626729
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0352
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- PhylomeDB:   A1SRV2
- ProtClustDB:   PRK07449
- BioCyc:   PING357804:PING_0352-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 64901; Mature: 64901

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITTLYKEPASFAHEFSNINLLWASLFIEELVRNGISDFCIAPGSRSTPLTLAADQHTEA
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCCEEEEECCCCCC
HTHVHFDERGLGFLALGLSLFSHKPVVIITTSGTAVANLYPAVIEAKLSAIPLIILSADR
CEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEECCC
PVELIDCGANQAIDQYRIFSHYPVFFAQIPSATTHIKPNYLLTTINQGLQQQRQTPAAIH
CEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHHCCCCEEE
FNIAFSEPLYPQTATLNYQGYLQSLKQWLIDKQPFSQYFQNKDSFQAASNTQLRDKKVLV
EEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHCCCCCCCCCEEEE
IAGRVTGINQAQAIAEFAALNNYPLLADLQSALTGNANNLHYYDLLLVNKQFTENLQQAD
EEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEEEECHHHHHCHHHHH
IIVQFGGKLISKRLSQFIEGFAGEYLLVDPGNTRIDPAHSLRKRFVCSATQWIKSLQNKI
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
PDIDKHWSQVLQQQNNYITKQIISPFLDNNLISEISVISALDKLLPADNPVFIGNSMPVR
CCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEE
LSDMFFRQNAALPFSNRGASGIDGLLATASGIAKSCASITTLLIGDTSFLHDLNSLALLK
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHH
QLQGPFVIIVFNNDGGAIFNLLPVPTQQKQDYYQLPHGLTFADSCRQFSIDYYQPESLDQ
HCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCHHHH
FVGDYQKSLQNRLSLIEICVKNDQTYNHLEYIKEQIKYATF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITTLYKEPASFAHEFSNINLLWASLFIEELVRNGISDFCIAPGSRSTPLTLAADQHTEA
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCCEEEEECCCCCC
HTHVHFDERGLGFLALGLSLFSHKPVVIITTSGTAVANLYPAVIEAKLSAIPLIILSADR
CEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEECCC
PVELIDCGANQAIDQYRIFSHYPVFFAQIPSATTHIKPNYLLTTINQGLQQQRQTPAAIH
CEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHHCCCCEEE
FNIAFSEPLYPQTATLNYQGYLQSLKQWLIDKQPFSQYFQNKDSFQAASNTQLRDKKVLV
EEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHCCCCCCCCCEEEE
IAGRVTGINQAQAIAEFAALNNYPLLADLQSALTGNANNLHYYDLLLVNKQFTENLQQAD
EEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEEEECHHHHHCHHHHH
IIVQFGGKLISKRLSQFIEGFAGEYLLVDPGNTRIDPAHSLRKRFVCSATQWIKSLQNKI
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
PDIDKHWSQVLQQQNNYITKQIISPFLDNNLISEISVISALDKLLPADNPVFIGNSMPVR
CCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEE
LSDMFFRQNAALPFSNRGASGIDGLLATASGIAKSCASITTLLIGDTSFLHDLNSLALLK
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHH
QLQGPFVIIVFNNDGGAIFNLLPVPTQQKQDYYQLPHGLTFADSCRQFSIDYYQPESLDQ
HCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCHHHH
FVGDYQKSLQNRLSLIEICVKNDQTYNHLEYIKEQIKYATF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA