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The map label for this gene is 119357699

Identifier: 119357699

GI number: 119357699

Start: 2211310

End: 2212200

Strand: Reverse

Name: 119357699

Synonym: Cpha266_1903

Alternate gene names: NA

Gene position: 2212200-2211310 (Counterclockwise)

Preceding gene: 119357700

Following gene: 119357698

Centisome position: 70.59

GC content: 53.87

Gene sequence:

>891_bases
ATGCCGTCGGAAACAGTTACCATCGCACTTCTTCAAACAACATCGTCAGAAAGACCGGAAGAGAACCTCGCCGAAGCGGA
TCGCCTTATCAGGAGCGCTGCTGCCGGCGGAGCACAGGTTATCTGCCTGCAGGAGCTGTTCACCACACGGTACTTCTGTC
AGATCGAGGATTATGAACCCTTTGCTTACGCTGAACCTGTTCCCGGTCCGACAACCCAGGCTTTGCAGGAACTGGCACGC
GAGCTTCAGGTCGTCATCGTCGCCTCGCTTTTTGAAGCGCGGGCCAGAGGTCTCTATCATAATACCGCTGCGGTTATCGA
TGCAGATGGCAGCTATCTCGGCAAGTACAGGAAAATGCATATTCCCGATGATCCCGGGTTTTACGAGAAGTTTTATTTCA
CCCCCGGAGATCTCGGTTACAAAGTTTTTAAAACCCGGTATGCAACCATCGGCGTTCTGATCTGCTGGGATCAATGGTAC
CCTGAAGCGGCAAGGCTGGTTGCGCTCAGGGGTGCCGAAATCATTTTTTATCCAACGGCCATAGGCTGGGCAGCCAGCGA
GATTTCCGACGAGGTACGCCGAGCGCAACGGACAGCATGGAAAACCATGCAGCTCAGCCATGCGGTTGCCAATGGCGTAT
TTGTCGCTGCGGCCAACAGGGTTGGTACTGAAGGTGAGCTTGAGTTCTGGGGAAACAGCTTTGTCTCTGATCCTTTTGGT
CAGGTTATTGCCGAAGCTCCCCATCAGAACGAAGCCGTTCTGCTTGCCCGGTGCGATCTCGGTCGTATCGGATACTACCG
TTCGCACTGGCCTTTTTTGCGTGATCGTCGCATTGAATCCTACGGGGATGTGCAGAAGCGCTACATAGATGCCGATAGCG
GACAGGGTTAG

Upstream 100 bases:

>100_bases
GGGCGTTGAACGATGTTCTTGCCGGTACGGGAAAAGTCATGACTATTCACGGCCTTCTTTTTGCCGCGGGGCTTCTCATT
CACTAACAACGATGATATCA

Downstream 100 bases:

>100_bases
GGGGGAGATCGTGTTAAGAGTTAAGGGTTAAGATGGAAGAGGGACGACTCGAATATCTTTTTAGCATCCGGAATACCCTT
TTTGCAGCCCGAATTTCTTT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPSETVTIALLQTTSSERPEENLAEADRLIRSAAAGGAQVICLQELFTTRYFCQIEDYEPFAYAEPVPGPTTQALQELAR
ELQVVIVASLFEARARGLYHNTAAVIDADGSYLGKYRKMHIPDDPGFYEKFYFTPGDLGYKVFKTRYATIGVLICWDQWY
PEAARLVALRGAEIIFYPTAIGWAASEISDEVRRAQRTAWKTMQLSHAVANGVFVAAANRVGTEGELEFWGNSFVSDPFG
QVIAEAPHQNEAVLLARCDLGRIGYYRSHWPFLRDRRIESYGDVQKRYIDADSGQG

Sequences:

>Translated_296_residues
MPSETVTIALLQTTSSERPEENLAEADRLIRSAAAGGAQVICLQELFTTRYFCQIEDYEPFAYAEPVPGPTTQALQELAR
ELQVVIVASLFEARARGLYHNTAAVIDADGSYLGKYRKMHIPDDPGFYEKFYFTPGDLGYKVFKTRYATIGVLICWDQWY
PEAARLVALRGAEIIFYPTAIGWAASEISDEVRRAQRTAWKTMQLSHAVANGVFVAAANRVGTEGELEFWGNSFVSDPFG
QVIAEAPHQNEAVLLARCDLGRIGYYRSHWPFLRDRRIESYGDVQKRYIDADSGQG
>Mature_295_residues
PSETVTIALLQTTSSERPEENLAEADRLIRSAAAGGAQVICLQELFTTRYFCQIEDYEPFAYAEPVPGPTTQALQELARE
LQVVIVASLFEARARGLYHNTAAVIDADGSYLGKYRKMHIPDDPGFYEKFYFTPGDLGYKVFKTRYATIGVLICWDQWYP
EAARLVALRGAEIIFYPTAIGWAASEISDEVRRAQRTAWKTMQLSHAVANGVFVAAANRVGTEGELEFWGNSFVSDPFGQ
VIAEAPHQNEAVLLARCDLGRIGYYRSHWPFLRDRRIESYGDVQKRYIDADSGQG

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=286, Percent_Identity=32.8671328671329, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI7706509, Length=274, Percent_Identity=32.4817518248175, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI5031947, Length=293, Percent_Identity=27.3037542662116, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI297632348, Length=293, Percent_Identity=27.6450511945392, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI297632350, Length=293, Percent_Identity=27.6450511945392, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI297632346, Length=189, Percent_Identity=28.042328042328, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17533173, Length=273, Percent_Identity=34.7985347985348, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17556280, Length=283, Percent_Identity=25.0883392226148, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323383, Length=296, Percent_Identity=27.7027027027027, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322335, Length=318, Percent_Identity=23.8993710691824, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21358471, Length=305, Percent_Identity=30.4918032786885, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21355835, Length=297, Percent_Identity=27.9461279461279, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17933642, Length=284, Percent_Identity=23.943661971831, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33185; Mature: 33053

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSETVTIALLQTTSSERPEENLAEADRLIRSAAAGGAQVICLQELFTTRYFCQIEDYEP
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCEEEEEEECCCC
FAYAEPVPGPTTQALQELARELQVVIVASLFEARARGLYHNTAAVIDADGSYLGKYRKMH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCEEC
IPDDPGFYEKFYFTPGDLGYKVFKTRYATIGVLICWDQWYPEAARLVALRGAEIIFYPTA
CCCCCCCCCEEEECCCCCCHHHHHHHHEEEEEEEEECCCCCHHHHEEEECCCEEEEECCC
IGWAASEISDEVRRAQRTAWKTMQLSHAVANGVFVAAANRVGTEGELEFWGNSFVSDPFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCCCCCCHHH
QVIAEAPHQNEAVLLARCDLGRIGYYRSHWPFLRDRRIESYGDVQKRYIDADSGQG
HHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
PSETVTIALLQTTSSERPEENLAEADRLIRSAAAGGAQVICLQELFTTRYFCQIEDYEP
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCEEEEEEECCCC
FAYAEPVPGPTTQALQELARELQVVIVASLFEARARGLYHNTAAVIDADGSYLGKYRKMH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCEEC
IPDDPGFYEKFYFTPGDLGYKVFKTRYATIGVLICWDQWYPEAARLVALRGAEIIFYPTA
CCCCCCCCCEEEECCCCCCHHHHHHHHEEEEEEEEECCCCCHHHHEEEECCCEEEEECCC
IGWAASEISDEVRRAQRTAWKTMQLSHAVANGVFVAAANRVGTEGELEFWGNSFVSDPFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCCCCCCHHH
QVIAEAPHQNEAVLLARCDLGRIGYYRSHWPFLRDRRIESYGDVQKRYIDADSGQG
HHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]