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The map label for this gene is bcr [H]

Identifier: 116688987

GI number: 116688987

Start: 1069923

End: 1071140

Strand: Direct

Name: bcr [H]

Synonym: Bcen2424_0964

Alternate gene names: 116688987

Gene position: 1069923-1071140 (Clockwise)

Preceding gene: 116688976

Following gene: 116688991

Centisome position: 30.71

GC content: 70.61

Gene sequence:

>1218_bases
ATGTCCCACGTCGTCCGGCGCCGGCCCGATGCCCGGCTGATCCTGTTGCTCGGCGCGCTCGCGGCCTGCGGGCCGATCGC
CACCGACATGTACCTGCCGAGCCTGCCGTCGATCGCCGCGGGCTTCTCCGTCAGCGCCGGCGCCGCGCAGCGCACGCTGA
CGAGCTTCATGGCCGGCTTCTCGATCGGCATGCTGCTGTACGGCCCGCTGTCCGACACGTGGGGCCGCCGCCCCGTGCTG
CTCGGCGGGATCGCGCTGTTCACGCTCGCGAGTATCGGCTGCTTCGTGGCGGGTTCGATCGACATGCTGATCGTCGTGCG
CTTTTTGCAGGCGCTCGGCGCCGGCGCCGCGTCGGTGCTCGCGCGGGCGATCGCGCGCGACGCGCACGAGCCGAGCGACG
CGGCGAAGGTGCTGTCGATGGTCGCGATCGTTACCGCGGTCGGGCCGCTGCTCGCGCCGCTGATCGGCGGCCAGATCCTG
CGTTTCTCCGGCTGGCGCGGCGTGTTCGTCGTGCTGGCCGTGTTCGGCGCGCTCTGCGCGACGACCGCGTACCTGCGCGT
GCCCGAAACCTGGCCGAAGGAGCGGCGCAAGAGCGCGGCCGTGCTCGCGTCGTTCGCGTCGTACGGGCGCATCCTGTCCG
ACCCCGTCGCATGGGGGCACATGCTGTGCGGCGGGATGGCGTTCGCATCGATGTTCTCGTACATCACCGCGACGCCGTTC
GTGTACATCGAGTATTTCCACGTGTCGCCGCAGCACTACGGGCTGCTGTTCGGCCTGAACGTCGTCGGGATCATGATCGG
CAACTTCGCGAACTCGCGGCTCGTCGGCCGCATCGGTTCGCTGCGCATCATCGCGGCCGCGTCGCTCGTGAGCTGCATCG
CGTCGCTCGCGGTCGCGTTCGTCGCGCTGACCGGATTGGGCGGGCTGTGGTCGATCGTCGTGTGCCTGTTCTTCGTCGTC
GGCGTGGTCGGGATCCTGTCCGCGAACTGCACGACCGACCTGATGCATCGCTATCCGCACAACGCGGGCGCGTCGGCGGC
CGTGTTCGGCGCGATGCAGCTCGCGCTCGGCGCGCTGGCGAGCGTCGCGATCGGCGCACTCGCGGACGGCACGCCGTTCG
CGATGGGCGTGACGATCGGTGTGTCGGGCCTGCTGTGCTTCGCCGGGCGCTATCTGGTGCTGCGCTGGCACGGCCGGCCG
GTGAAAGCGGGCGCCTGA

Upstream 100 bases:

>100_bases
CGCGTACAATCATGGGCCTGAACGCGCCGCTTGCGGCGCGGCTTCCCGTCTTCACCGCCACGCGTGCCCCGATGCGCGCG
GGCTCCCATTCCGGATACCC

Downstream 100 bases:

>100_bases
ACCGCGCCGCCCGCCGGGTCGCACAATGAAAAACGCCACCGCGCGCGATGCGCCGGTGGCGTTTTTCGTTGGGACGCGTG
AAGCGGCTGACGCCGCGCCA

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAAGFSVSAGAAQRTLTSFMAGFSIGMLLYGPLSDTWGRRPVL
LGGIALFTLASIGCFVAGSIDMLIVVRFLQALGAGAASVLARAIARDAHEPSDAAKVLSMVAIVTAVGPLLAPLIGGQIL
RFSGWRGVFVVLAVFGALCATTAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFANSRLVGRIGSLRIIAAASLVSCIASLAVAFVALTGLGGLWSIVVCLFFVV
GVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALASVAIGALADGTPFAMGVTIGVSGLLCFAGRYLVLRWHGRP
VKAGA

Sequences:

>Translated_405_residues
MSHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAAGFSVSAGAAQRTLTSFMAGFSIGMLLYGPLSDTWGRRPVL
LGGIALFTLASIGCFVAGSIDMLIVVRFLQALGAGAASVLARAIARDAHEPSDAAKVLSMVAIVTAVGPLLAPLIGGQIL
RFSGWRGVFVVLAVFGALCATTAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFANSRLVGRIGSLRIIAAASLVSCIASLAVAFVALTGLGGLWSIVVCLFFVV
GVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALASVAIGALADGTPFAMGVTIGVSGLLCFAGRYLVLRWHGRP
VKAGA
>Mature_404_residues
SHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAAGFSVSAGAAQRTLTSFMAGFSIGMLLYGPLSDTWGRRPVLL
GGIALFTLASIGCFVAGSIDMLIVVRFLQALGAGAASVLARAIARDAHEPSDAAKVLSMVAIVTAVGPLLAPLIGGQILR
FSGWRGVFVVLAVFGALCATTAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPFV
YIEYFHVSPQHYGLLFGLNVVGIMIGNFANSRLVGRIGSLRIIAAASLVSCIASLAVAFVALTGLGGLWSIVVCLFFVVG
VVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALASVAIGALADGTPFAMGVTIGVSGLLCFAGRYLVLRWHGRPV
KAGA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=397, Percent_Identity=35.7682619647355, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI48994889, Length=395, Percent_Identity=29.620253164557, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790146, Length=202, Percent_Identity=34.6534653465347, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082312, Length=344, Percent_Identity=28.1976744186047, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787065, Length=257, Percent_Identity=27.6264591439689, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790794, Length=223, Percent_Identity=24.6636771300448, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790195, Length=138, Percent_Identity=29.7101449275362, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=168, Percent_Identity=27.9761904761905, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=178, Percent_Identity=24.7191011235955, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322071, Length=165, Percent_Identity=28.4848484848485, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42038; Mature: 41907

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAAGFSVSAGAAQRTLTSFMAGF
CCCCHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHH
SIGMLLYGPLSDTWGRRPVLLGGIALFTLASIGCFVAGSIDMLIVVRFLQALGAGAASVL
HHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHH
ARAIARDAHEPSDAAKVLSMVAIVTAVGPLLAPLIGGQILRFSGWRGVFVVLAVFGALCA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHH
TTAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCE
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFANSRLVGRIGSLRIIAAASLVSCIASLAVAF
EEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VALTGLGGLWSIVVCLFFVVGVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SVAIGALADGTPFAMGVTIGVSGLLCFAGRYLVLRWHGRPVKAGA
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
SHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAAGFSVSAGAAQRTLTSFMAGF
CCCHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHH
SIGMLLYGPLSDTWGRRPVLLGGIALFTLASIGCFVAGSIDMLIVVRFLQALGAGAASVL
HHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHH
ARAIARDAHEPSDAAKVLSMVAIVTAVGPLLAPLIGGQILRFSGWRGVFVVLAVFGALCA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHH
TTAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCE
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFANSRLVGRIGSLRIIAAASLVSCIASLAVAF
EEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VALTGLGGLWSIVVCLFFVVGVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SVAIGALADGTPFAMGVTIGVSGLLCFAGRYLVLRWHGRPVKAGA
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]