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The map label for this gene is kinE [H]

Identifier: 116619533

GI number: 116619533

Start: 494026

End: 495624

Strand: Direct

Name: kinE [H]

Synonym: Acid_0392

Alternate gene names: 116619533

Gene position: 494026-495624 (Clockwise)

Preceding gene: 116619531

Following gene: 116619534

Centisome position: 4.96

GC content: 61.66

Gene sequence:

>1599_bases
ATGCCGCGACGTCTGGCGCGGAAGCTGATGCTCTCGATCACCGTCATTGTGATCATCGTGGCGGCGGTTTCCGGGCTGGT
GAACGTAAAAACCGAGGAGCAGCAGCTCCTGAACACCATGATCCTGGGAGCGGATCAACTCTCCAAGGGCATCACCAGCG
CGACGTGGCACGCCATGCTGGACGATCACCGTGAAGCCGCGTACCAGGTAATGCAGACGATCGCGCTCAAGCAGGGGATC
GACCGTATCCGCATGTTCAACCGGGCCGGCCAGGTGATGTTCTCCACCAATGCCGAAGACCGGGCCAGCGCGACCGAGGC
TCTGCCGGAGACCGACCATCCCGACCTGCCTTCACGTGTTCGGATTTTTCGTGGCGCTAACGGGGGGCGGCGGCTGGCTA
TGGCCACGAAGATCTACAACGAGCCTTCCTGCAGCCAGGCGGAGTGCCACGCGCATCCGGCGGAGATGAAAGTGCTCGGC
GTGCTCGACCTGCGCCTGAATCTGGAGAACGTGGATCAGGAGGTCGCCGGGATGAAGTATCGCGTGATGCTGGTGACCGC
CGTCGAGGTAACGCTGATCAGTATTTTCATCATTTACTTTACACGCCGCTTCCTCGGCCGGCCGATCGAGAAGTTGATCG
AAGGGACCAAGGCGATCAGTCAAATGGAACTGGACAAGCCGCTCGACATCATTAATAGCAGCGAGGAGCTCGACGAGCTG
GCGCGCTCTTTCGACGTGATGCGCGACCGGTTGCGTTCGGCGCTGGCGGAAATCAATCAGTTCACGCAGAATCTGGAAAC
CAAAGTCGAGGAGCGCACGCAGCAACTGAAGGCCGCGCAGAAAAAGCTGCTGCAGAGCGACCGGCTGGCGTCGCTCGGTC
AGCTTTCGGCATCGGTGGCGCACGAGATCAACAATCCGGTTTCCGGCGTGCTCAATCTTTCGATGCTGATGCAGCGCATG
TTAAAGGACGACGGCATTCCGCCGGAGCGACTCGCGGAATTTCGCAAGTACCTGGGGCAGGTGACCAGCGAAACTGCGCG
GGTGGGCCGGATTGTCAGCGACCTGCTGGCGTTCTCGCGGCGCGGCAAGCCGCAGCAGGCGCCGGCCGACATCAACCGCA
TCGTGGCGATGACGCTTTCGCTGGTGGAACACAAGATGAAGCTGAGCAACGTGGCGGTGAAGGTTACGCTGTGCGAGCAG
CTTCCTCCGGCGCATTGCGACCCTTCGCAGATTCAGCAGGTGGTGCTCAACCTTGTGCTCAACGCGGCTGAGGCTACTCA
GGCCAGGCCGGACCGGCACGTCACGGTGTCCACTTCGAGCAGCGAAGGCATGGCGCAGATTTTTGTGTCCGACAATGGCG
AGGGCATTCCGCCGGAGAACCTATCGAAGATTTTCGACCCCTTTTTCACGACCAAGCCCGAGGGCAAGGGCGTGGGACTC
GGGCTGGCCGTCACGTATGGAATCATCGAGGCGCACAGCGGGGATATCGAGGTGAAGAGCACGGTGGGCAAAGGCACGAC
GTTTATCGTGTCGCTGCCGCTGGAGGCGCGCGCGCCGCGCGCCGAGCCGCCGCTTGCGGTACTGGCGCGGCGGGAATGA

Upstream 100 bases:

>100_bases
CCAACGAGAATGGCCCAGGCATGCATGGTCCCTATTCTACGCCGCTCAAGAGTGCCGTCCACGCCCCGCGAGACCAGCTA
CAATACTTCCAGGTCTGAAT

Downstream 100 bases:

>100_bases
AGTATTTGTACGTGGGCGCGACGGAAGAGGTGGACCGCGAGGCCGTGGCGGCCCTTTCGCAGCGGGTCGAAACCGAATTC
GGCCTGCCGGTGCGCGGCAT

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MPRRLARKLMLSITVIVIIVAAVSGLVNVKTEEQQLLNTMILGADQLSKGITSATWHAMLDDHREAAYQVMQTIALKQGI
DRIRMFNRAGQVMFSTNAEDRASATEALPETDHPDLPSRVRIFRGANGGRRLAMATKIYNEPSCSQAECHAHPAEMKVLG
VLDLRLNLENVDQEVAGMKYRVMLVTAVEVTLISIFIIYFTRRFLGRPIEKLIEGTKAISQMELDKPLDIINSSEELDEL
ARSFDVMRDRLRSALAEINQFTQNLETKVEERTQQLKAAQKKLLQSDRLASLGQLSASVAHEINNPVSGVLNLSMLMQRM
LKDDGIPPERLAEFRKYLGQVTSETARVGRIVSDLLAFSRRGKPQQAPADINRIVAMTLSLVEHKMKLSNVAVKVTLCEQ
LPPAHCDPSQIQQVVLNLVLNAAEATQARPDRHVTVSTSSSEGMAQIFVSDNGEGIPPENLSKIFDPFFTTKPEGKGVGL
GLAVTYGIIEAHSGDIEVKSTVGKGTTFIVSLPLEARAPRAEPPLAVLARRE

Sequences:

>Translated_532_residues
MPRRLARKLMLSITVIVIIVAAVSGLVNVKTEEQQLLNTMILGADQLSKGITSATWHAMLDDHREAAYQVMQTIALKQGI
DRIRMFNRAGQVMFSTNAEDRASATEALPETDHPDLPSRVRIFRGANGGRRLAMATKIYNEPSCSQAECHAHPAEMKVLG
VLDLRLNLENVDQEVAGMKYRVMLVTAVEVTLISIFIIYFTRRFLGRPIEKLIEGTKAISQMELDKPLDIINSSEELDEL
ARSFDVMRDRLRSALAEINQFTQNLETKVEERTQQLKAAQKKLLQSDRLASLGQLSASVAHEINNPVSGVLNLSMLMQRM
LKDDGIPPERLAEFRKYLGQVTSETARVGRIVSDLLAFSRRGKPQQAPADINRIVAMTLSLVEHKMKLSNVAVKVTLCEQ
LPPAHCDPSQIQQVVLNLVLNAAEATQARPDRHVTVSTSSSEGMAQIFVSDNGEGIPPENLSKIFDPFFTTKPEGKGVGL
GLAVTYGIIEAHSGDIEVKSTVGKGTTFIVSLPLEARAPRAEPPLAVLARRE
>Mature_531_residues
PRRLARKLMLSITVIVIIVAAVSGLVNVKTEEQQLLNTMILGADQLSKGITSATWHAMLDDHREAAYQVMQTIALKQGID
RIRMFNRAGQVMFSTNAEDRASATEALPETDHPDLPSRVRIFRGANGGRRLAMATKIYNEPSCSQAECHAHPAEMKVLGV
LDLRLNLENVDQEVAGMKYRVMLVTAVEVTLISIFIIYFTRRFLGRPIEKLIEGTKAISQMELDKPLDIINSSEELDELA
RSFDVMRDRLRSALAEINQFTQNLETKVEERTQQLKAAQKKLLQSDRLASLGQLSASVAHEINNPVSGVLNLSMLMQRML
KDDGIPPERLAEFRKYLGQVTSETARVGRIVSDLLAFSRRGKPQQAPADINRIVAMTLSLVEHKMKLSNVAVKVTLCEQL
PPAHCDPSQIQQVVLNLVLNAAEATQARPDRHVTVSTSSSEGMAQIFVSDNGEGIPPENLSKIFDPFFTTKPEGKGVGLG
LAVTYGIIEAHSGDIEVKSTVGKGTTFIVSLPLEARAPRAEPPLAVLARRE

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=245, Percent_Identity=35.9183673469388, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=35.7142857142857, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081816, Length=366, Percent_Identity=25.4098360655738, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI48994928, Length=231, Percent_Identity=31.1688311688312, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI145693157, Length=262, Percent_Identity=30.5343511450382, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1790300, Length=249, Percent_Identity=30.5220883534137, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786783, Length=347, Percent_Identity=22.7665706051873, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789149, Length=383, Percent_Identity=23.7597911227154, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786912, Length=259, Percent_Identity=25.8687258687259, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790346, Length=287, Percent_Identity=24.390243902439, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=28.3185840707965, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787894, Length=240, Percent_Identity=26.25, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788393, Length=316, Percent_Identity=21.8354430379747, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58668; Mature: 58537

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRLARKLMLSITVIVIIVAAVSGLVNVKTEEQQLLNTMILGADQLSKGITSATWHAML
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCHHHHHHHHH
DDHREAAYQVMQTIALKQGIDRIRMFNRAGQVMFSTNAEDRASATEALPETDHPDLPSRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCHHH
RIFRGANGGRRLAMATKIYNEPSCSQAECHAHPAEMKVLGVLDLRLNLENVDQEVAGMKY
EEEECCCCCCEEEHHHHHCCCCCCCCCHHCCCCCCEEEEEEEEEEECHHHHHHHHHCCEE
RVMLVTAVEVTLISIFIIYFTRRFLGRPIEKLIEGTKAISQMELDKPLDIINSSEELDEL
EEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHH
ARSFDVMRDRLRSALAEINQFTQNLETKVEERTQQLKAAQKKLLQSDRLASLGQLSASVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEINNPVSGVLNLSMLMQRMLKDDGIPPERLAEFRKYLGQVTSETARVGRIVSDLLAFSR
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGKPQQAPADINRIVAMTLSLVEHKMKLSNVAVKVTLCEQLPPAHCDPSQIQQVVLNLVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHCCCCCCCCHHHHHHHHHHHHH
NAAEATQARPDRHVTVSTSSSEGMAQIFVSDNGEGIPPENLSKIFDPFFTTKPEGKGVGL
HHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
GLAVTYGIIEAHSGDIEVKSTVGKGTTFIVSLPLEARAPRAEPPLAVLARRE
HHHHHHHHHEECCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
PRRLARKLMLSITVIVIIVAAVSGLVNVKTEEQQLLNTMILGADQLSKGITSATWHAML
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCHHHHHHHHH
DDHREAAYQVMQTIALKQGIDRIRMFNRAGQVMFSTNAEDRASATEALPETDHPDLPSRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCHHH
RIFRGANGGRRLAMATKIYNEPSCSQAECHAHPAEMKVLGVLDLRLNLENVDQEVAGMKY
EEEECCCCCCEEEHHHHHCCCCCCCCCHHCCCCCCEEEEEEEEEEECHHHHHHHHHCCEE
RVMLVTAVEVTLISIFIIYFTRRFLGRPIEKLIEGTKAISQMELDKPLDIINSSEELDEL
EEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHH
ARSFDVMRDRLRSALAEINQFTQNLETKVEERTQQLKAAQKKLLQSDRLASLGQLSASVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEINNPVSGVLNLSMLMQRMLKDDGIPPERLAEFRKYLGQVTSETARVGRIVSDLLAFSR
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGKPQQAPADINRIVAMTLSLVEHKMKLSNVAVKVTLCEQLPPAHCDPSQIQQVVLNLVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHCCCCCCCCHHHHHHHHHHHHH
NAAEATQARPDRHVTVSTSSSEGMAQIFVSDNGEGIPPENLSKIFDPFFTTKPEGKGVGL
HHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
GLAVTYGIIEAHSGDIEVKSTVGKGTTFIVSLPLEARAPRAEPPLAVLARRE
HHHHHHHHHEECCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]