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The map label for this gene is puuA [H]

Identifier: 103486609

GI number: 103486609

Start: 1157794

End: 1159140

Strand: Reverse

Name: puuA [H]

Synonym: Sala_1121

Alternate gene names: 103486609

Gene position: 1159140-1157794 (Counterclockwise)

Preceding gene: 103486611

Following gene: 103486608

Centisome position: 34.65

GC content: 61.1

Gene sequence:

>1347_bases
ATGTCAGTCAATCTGGAACAATGGATCAGCGAGCACAAGATCGACGAAGTCGAATGTATCGTCCCCGACATCAATGGGGT
TCAGCGCGGCAAGGTGTTGCCGGCGCGCAAGTTTCTCACCTCGGTCAAGGACAAGACGCTGCGCATTCCCGGCAGCGTCT
TCATCTGCACGATCGACGGTCATTACCCCGAGGACATCGACGATGTCTGGGACAAGGACCCGGACAAGATATTGATCGCC
GATCCCGACACGATCTGCGTCGCGCCGGGCTTTACCTCGCCGACCGCTTTCGTCATCGCCGACGCCTTCAACGGCGACGG
GACCGAGGTCGACATCGCGCCGCGCACGATCCTCAAAAAAGTGCTGGGCCTCTACGAAGACAGGGGCTGGAAACCGATCA
TTGCGCCCGAGGTGGAGTTTTACCTCGTTTCGCAGAACACCGATCCCGATTTCCCGCTGACTCCGCCCACCGGCCAGTCG
GGCCGTGCCGAAACGGCGAGCCAGCCCTATGGGCTGGAGGCGATGAACGAATATGAAGACATCATCGATCATATCTATGA
CGATTGCGAGCTGATGGGGCTCAATATCGACACGATGATTCACGAAATGGGCGCCGCGCAGCTGGAGGTGAACTTCAACC
ATGGCGATCCGCTGCGGCTCGCCGACGAGGTCTTCCTGTTCAAACGCGTCGTCCGCAATGTGGCGCGGCAACATGACGTC
TATGCCACTTTCATGGCGAACCCGATGGCGGGCCAGCCAGGCAGCGCGATGCACATCCATCAGTCGGTCATCGATGCCGA
TACGGGTCGCAACCTGTTCGCGACCGCGAATGGCCGCGACAGCGCCGCCTTTCGGTCCTTTGTCGCGGGGCTCGTCCGTT
TCATGCCGCAAATTGCGCCGCTCTGGGCGCCCAATGTGAACAGCTTTCGCCGGATGCGCCCCGACAGCGCGGCGCCGATC
AACGTCCAGTGGGGCGCCGACAACCGCTCGTGCGGTTTTCGTATCCCGGTGTCCGACAAGCACAACCGCCGCGTCGAAAA
CCGCCTGCCCGGCGCCGACAGCAATCCCTATCTCGCGATCGCCGCGTCGCTCGTATGCGGCTATATCGGCATGGTCGACC
GGATGGTCCCGCCCAAGCCGATCACCGGCAGCGCCTATAATCGCGCACGCACCCTGCCGCGCACGCTGGAGGCCGCGCTC
GACCGTTTCGCCTCGTGCAAAAAGGTCCGCAACCTGCTCGGCGACGATTTCTTCGAGATTTTCTATGCCGTGAAGGATTA
TGAGCTGTTCAATTACCAGTCGGTGGTGTCGAGCTGGGAGCGCGAACATCTGCTGATGCGGGTCTGA

Upstream 100 bases:

>100_bases
GCTGGCAATGGACAAAACCGGCCCTCTGGCGCTTCACCCGACTTCACGATAGGCTGTCCGCTCCACAAACGGGCCGGCTC
CTCGGCCCAATCGGGGGCGC

Downstream 100 bases:

>100_bases
ACCGCCGATGATATTCTGCCCATCCTTCCCGTTCGCGTCGAGCGAGGTCGAGACGCCCATCGCGACGGCGCGGCGTTTCG
ACTTCGCTCGACGCGAGCCG

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSVNLEQWISEHKIDEVECIVPDINGVQRGKVLPARKFLTSVKDKTLRIPGSVFICTIDGHYPEDIDDVWDKDPDKILIA
DPDTICVAPGFTSPTAFVIADAFNGDGTEVDIAPRTILKKVLGLYEDRGWKPIIAPEVEFYLVSQNTDPDFPLTPPTGQS
GRAETASQPYGLEAMNEYEDIIDHIYDDCELMGLNIDTMIHEMGAAQLEVNFNHGDPLRLADEVFLFKRVVRNVARQHDV
YATFMANPMAGQPGSAMHIHQSVIDADTGRNLFATANGRDSAAFRSFVAGLVRFMPQIAPLWAPNVNSFRRMRPDSAAPI
NVQWGADNRSCGFRIPVSDKHNRRVENRLPGADSNPYLAIAASLVCGYIGMVDRMVPPKPITGSAYNRARTLPRTLEAAL
DRFASCKKVRNLLGDDFFEIFYAVKDYELFNYQSVVSSWEREHLLMRV

Sequences:

>Translated_448_residues
MSVNLEQWISEHKIDEVECIVPDINGVQRGKVLPARKFLTSVKDKTLRIPGSVFICTIDGHYPEDIDDVWDKDPDKILIA
DPDTICVAPGFTSPTAFVIADAFNGDGTEVDIAPRTILKKVLGLYEDRGWKPIIAPEVEFYLVSQNTDPDFPLTPPTGQS
GRAETASQPYGLEAMNEYEDIIDHIYDDCELMGLNIDTMIHEMGAAQLEVNFNHGDPLRLADEVFLFKRVVRNVARQHDV
YATFMANPMAGQPGSAMHIHQSVIDADTGRNLFATANGRDSAAFRSFVAGLVRFMPQIAPLWAPNVNSFRRMRPDSAAPI
NVQWGADNRSCGFRIPVSDKHNRRVENRLPGADSNPYLAIAASLVCGYIGMVDRMVPPKPITGSAYNRARTLPRTLEAAL
DRFASCKKVRNLLGDDFFEIFYAVKDYELFNYQSVVSSWEREHLLMRV
>Mature_447_residues
SVNLEQWISEHKIDEVECIVPDINGVQRGKVLPARKFLTSVKDKTLRIPGSVFICTIDGHYPEDIDDVWDKDPDKILIAD
PDTICVAPGFTSPTAFVIADAFNGDGTEVDIAPRTILKKVLGLYEDRGWKPIIAPEVEFYLVSQNTDPDFPLTPPTGQSG
RAETASQPYGLEAMNEYEDIIDHIYDDCELMGLNIDTMIHEMGAAQLEVNFNHGDPLRLADEVFLFKRVVRNVARQHDVY
ATFMANPMAGQPGSAMHIHQSVIDADTGRNLFATANGRDSAAFRSFVAGLVRFMPQIAPLWAPNVNSFRRMRPDSAAPIN
VQWGADNRSCGFRIPVSDKHNRRVENRLPGADSNPYLAIAASLVCGYIGMVDRMVPPKPITGSAYNRARTLPRTLEAALD
RFASCKKVRNLLGDDFFEIFYAVKDYELFNYQSVVSSWEREHLLMRV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=443, Percent_Identity=24.3792325056433, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI87081870, Length=444, Percent_Identity=32.6576576576577, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790301, Length=430, Percent_Identity=27.6744186046512, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50111; Mature: 49980

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNLEQWISEHKIDEVECIVPDINGVQRGKVLPARKFLTSVKDKTLRIPGSVFICTIDG
CCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEEECC
HYPEDIDDVWDKDPDKILIADPDTICVAPGFTSPTAFVIADAFNGDGTEVDIAPRTILKK
CCCCCHHHHHCCCCCEEEEECCCEEEEECCCCCCCEEEEEEEECCCCCEEECCHHHHHHH
VLGLYEDRGWKPIIAPEVEFYLVSQNTDPDFPLTPPTGQSGRAETASQPYGLEAMNEYED
HHHHHHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
IIDHIYDDCELMGLNIDTMIHEMGAAQLEVNFNHGDPLRLADEVFLFKRVVRNVARQHDV
HHHHHHCCCEEECCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCE
YATFMANPMAGQPGSAMHIHQSVIDADTGRNLFATANGRDSAAFRSFVAGLVRFMPQIAP
EEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCC
LWAPNVNSFRRMRPDSAAPINVQWGADNRSCGFRIPVSDKHNRRVENRLPGADSNPYLAI
CCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCCCCCCCHHHHCCCCCCCCCHHHH
AASLVCGYIGMVDRMVPPKPITGSAYNRARTLPRTLEAALDRFASCKKVRNLLGDDFFEI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FYAVKDYELFNYQSVVSSWEREHLLMRV
HHHHHCCHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SVNLEQWISEHKIDEVECIVPDINGVQRGKVLPARKFLTSVKDKTLRIPGSVFICTIDG
CCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEEECC
HYPEDIDDVWDKDPDKILIADPDTICVAPGFTSPTAFVIADAFNGDGTEVDIAPRTILKK
CCCCCHHHHHCCCCCEEEEECCCEEEEECCCCCCCEEEEEEEECCCCCEEECCHHHHHHH
VLGLYEDRGWKPIIAPEVEFYLVSQNTDPDFPLTPPTGQSGRAETASQPYGLEAMNEYED
HHHHHHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
IIDHIYDDCELMGLNIDTMIHEMGAAQLEVNFNHGDPLRLADEVFLFKRVVRNVARQHDV
HHHHHHCCCEEECCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCE
YATFMANPMAGQPGSAMHIHQSVIDADTGRNLFATANGRDSAAFRSFVAGLVRFMPQIAP
EEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCC
LWAPNVNSFRRMRPDSAAPINVQWGADNRSCGFRIPVSDKHNRRVENRLPGADSNPYLAI
CCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCCCCCCCHHHHCCCCCCCCCHHHH
AASLVCGYIGMVDRMVPPKPITGSAYNRARTLPRTLEAALDRFASCKKVRNLLGDDFFEI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FYAVKDYELFNYQSVVSSWEREHLLMRV
HHHHHCCHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]