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The map label for this gene is puuB [H]

Identifier: 103486608

GI number: 103486608

Start: 1156409

End: 1157674

Strand: Reverse

Name: puuB [H]

Synonym: Sala_1120

Alternate gene names: 103486608

Gene position: 1157674-1156409 (Counterclockwise)

Preceding gene: 103486609

Following gene: 103486607

Centisome position: 34.61

GC content: 66.19

Gene sequence:

>1266_bases
ATGAGCGACCCGCTGAACCACAGCTATTACGCCGCGACCGCGCACGAATGGCGGCCGCGCGATCATATTGAGGACGATTT
CAGCTGCGACGTTGCGGTCATCGGCGGTGGCTTCACCGGGCTGAGCGCCGCGCTCGCCTGCGCCGAGCGCGGTTTTTCGG
TCATTTTGGTCGAAGCCGAGCATGTCGGCTTCGGCGCCTCGGGGCGCAACGGGGGGCAGTTGATCCCCGGATTGCGCTGG
ACCGCATCCGAACTGGACACCGAGTTCGGCCACGAGCGCGCCGACGCGCTGTTCGACCTGTGCTGGCGCGACAACCGCGT
GAAGGCCCGGATCGCAAAGCATGGCATCGACTGCGACCTCAAGTCCGGGCACCTTGAGGCGGCGTGGACGCCGGGCGACT
TCGACGCGATGCGGCGCGAGGCGGACTATCTGGCGACGCGTTTCGGTTACGAAACCGAGGTTGTCGAAAAGGCGGGGATG
GACGCGCATGTCGCCAGTCCGCGCTATCACGGCGGCGTTTATGACGCCCGCGCCGGGCATTTTCACCCGCTGAACTACGC
CGTCGGTCTGGCGAAGGCCGCCGAGGCGGCAGGAGTCCGGATTTTTGAAGGCCGGCGCGCCACCTCGATCCGCGCGGGCG
ATCCCGGCCAACTGCTGCTCGGCAAGGTGGCGGTCGAAGCGCGCTATTTCATCGACGCCACCGACAGCTGGATCGGCGAC
CTCGAACCCGATCTCGGCCGCTACACCGTGCCGATCATGAACTATAATATCGCGACCGCGCCGCTCGCCAACGCCGACGA
ACTGCTGCCAAGCGACGCCGCGGTCGCCGACAGCCGCTTCGTGCTCAACTATTTCCGCCTGTCCGCCGACAAGCGCCTCA
TATTCGGCGGGGGCGAGCGCTATTCGCAGACGCCGCCGCGCGACATCGCCGCATTCGTGCGGCCCTTCATGACCGGAGTG
TTCCCGCAAATCGCCGACGCGAAGATCGATTATGGCTGGGGCGGCGCGGTTGGGGTGACGATGAACCGCCTGCCGCATAT
CGGACGGCGCGGAAACATGTTTTTCGCGCACGGCTTTTCGGGGCACGGCGCGCTGGTGACGACGCTTGCGGGCGAACTCA
TCGCCGAAGCAATGGCGGGGACCGCCGAGCGCTTTGACCTGCTCGCGAACCTTCCCTCCCGCCCCTTCCCCGGAGGCAAC
TGGCTTCAACGCCCGCTCGCGACGCTGGGACTGCTTTTCTATGCATTGAAGGACCGGCTCGGATGA

Upstream 100 bases:

>100_bases
CCATCCTTCCCGTTCGCGTCGAGCGAGGTCGAGACGCCCATCGCGACGGCGCGGCGTTTCGACTTCGCTCGACGCGAGCC
GGATTAGGGGTGGCGTCGGA

Downstream 100 bases:

>100_bases
TCGCCGGGGCCGCGATCGCGCGCGTCGCGGCGCGCGAGGCGGAGCGCTTTCGCACCCTCAATCCGCGCGCTTTCGCGCAT
CATGCGGCAGCAAAAGGCTG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSDPLNHSYYAATAHEWRPRDHIEDDFSCDVAVIGGGFTGLSAALACAERGFSVILVEAEHVGFGASGRNGGQLIPGLRW
TASELDTEFGHERADALFDLCWRDNRVKARIAKHGIDCDLKSGHLEAAWTPGDFDAMRREADYLATRFGYETEVVEKAGM
DAHVASPRYHGGVYDARAGHFHPLNYAVGLAKAAEAAGVRIFEGRRATSIRAGDPGQLLLGKVAVEARYFIDATDSWIGD
LEPDLGRYTVPIMNYNIATAPLANADELLPSDAAVADSRFVLNYFRLSADKRLIFGGGERYSQTPPRDIAAFVRPFMTGV
FPQIADAKIDYGWGGAVGVTMNRLPHIGRRGNMFFAHGFSGHGALVTTLAGELIAEAMAGTAERFDLLANLPSRPFPGGN
WLQRPLATLGLLFYALKDRLG

Sequences:

>Translated_421_residues
MSDPLNHSYYAATAHEWRPRDHIEDDFSCDVAVIGGGFTGLSAALACAERGFSVILVEAEHVGFGASGRNGGQLIPGLRW
TASELDTEFGHERADALFDLCWRDNRVKARIAKHGIDCDLKSGHLEAAWTPGDFDAMRREADYLATRFGYETEVVEKAGM
DAHVASPRYHGGVYDARAGHFHPLNYAVGLAKAAEAAGVRIFEGRRATSIRAGDPGQLLLGKVAVEARYFIDATDSWIGD
LEPDLGRYTVPIMNYNIATAPLANADELLPSDAAVADSRFVLNYFRLSADKRLIFGGGERYSQTPPRDIAAFVRPFMTGV
FPQIADAKIDYGWGGAVGVTMNRLPHIGRRGNMFFAHGFSGHGALVTTLAGELIAEAMAGTAERFDLLANLPSRPFPGGN
WLQRPLATLGLLFYALKDRLG
>Mature_420_residues
SDPLNHSYYAATAHEWRPRDHIEDDFSCDVAVIGGGFTGLSAALACAERGFSVILVEAEHVGFGASGRNGGQLIPGLRWT
ASELDTEFGHERADALFDLCWRDNRVKARIAKHGIDCDLKSGHLEAAWTPGDFDAMRREADYLATRFGYETEVVEKAGMD
AHVASPRYHGGVYDARAGHFHPLNYAVGLAKAAEAAGVRIFEGRRATSIRAGDPGQLLLGKVAVEARYFIDATDSWIGDL
EPDLGRYTVPIMNYNIATAPLANADELLPSDAAVADSRFVLNYFRLSADKRLIFGGGERYSQTPPRDIAAFVRPFMTGVF
PQIADAKIDYGWGGAVGVTMNRLPHIGRRGNMFFAHGFSGHGALVTTLAGELIAEAMAGTAERFDLLANLPSRPFPGGNW
LQRPLATLGLLFYALKDRLG

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=419, Percent_Identity=39.1408114558473, Blast_Score=312, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 45733; Mature: 45602

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPLNHSYYAATAHEWRPRDHIEDDFSCDVAVIGGGFTGLSAALACAERGFSVILVEAE
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEEC
HVGFGASGRNGGQLIPGLRWTASELDTEFGHERADALFDLCWRDNRVKARIAKHGIDCDL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
KSGHLEAAWTPGDFDAMRREADYLATRFGYETEVVEKAGMDAHVASPRYHGGVYDARAGH
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEECCCCCCCCCCCCCCCC
FHPLNYAVGLAKAAEAAGVRIFEGRRATSIRAGDPGQLLLGKVAVEARYFIDATDSWIGD
CCCHHHHHHHHHHHHHCCEEEECCCCCCEEECCCCCHHHHHHHEEEEEEEEECCCCCCCC
LEPDLGRYTVPIMNYNIATAPLANADELLPSDAAVADSRFVLNYFRLSADKRLIFGGGER
CCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCHHHHCHHHHHHHHHHCCCCEEEEECCCC
YSQTPPRDIAAFVRPFMTGVFPQIADAKIDYGWGGAVGVTMNRLPHIGRRGNMFFAHGFS
CCCCCHHHHHHHHHHHHHHCCHHHHCCEEECCCCCEEEHHHHHCCCCCCCCCEEEEECCC
GHGALVTTLAGELIAEAMAGTAERFDLLANLPSRPFPGGNWLQRPLATLGLLFYALKDRL
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure 
SDPLNHSYYAATAHEWRPRDHIEDDFSCDVAVIGGGFTGLSAALACAERGFSVILVEAE
CCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEEC
HVGFGASGRNGGQLIPGLRWTASELDTEFGHERADALFDLCWRDNRVKARIAKHGIDCDL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
KSGHLEAAWTPGDFDAMRREADYLATRFGYETEVVEKAGMDAHVASPRYHGGVYDARAGH
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEECCCCCCCCCCCCCCCC
FHPLNYAVGLAKAAEAAGVRIFEGRRATSIRAGDPGQLLLGKVAVEARYFIDATDSWIGD
CCCHHHHHHHHHHHHHCCEEEECCCCCCEEECCCCCHHHHHHHEEEEEEEEECCCCCCCC
LEPDLGRYTVPIMNYNIATAPLANADELLPSDAAVADSRFVLNYFRLSADKRLIFGGGER
CCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCHHHHCHHHHHHHHHHCCCCEEEEECCCC
YSQTPPRDIAAFVRPFMTGVFPQIADAKIDYGWGGAVGVTMNRLPHIGRRGNMFFAHGFS
CCCCCHHHHHHHHHHHHHHCCHHHHCCEEECCCCCEEEHHHHHCCCCCCCCCEEEEECCC
GHGALVTTLAGELIAEAMAGTAERFDLLANLPSRPFPGGNWLQRPLATLGLLFYALKDRL
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]