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The map label for this gene is pth

Identifier: 239618099

GI number: 239618099

Start: 1859501

End: 1860073

Strand: Reverse

Name: pth

Synonym: Kole_1731

Alternate gene names: 239618099

Gene position: 1860073-1859501 (Counterclockwise)

Preceding gene: 239618100

Following gene: 239618098

Centisome position: 80.8

GC content: 45.72

Gene sequence:

>573_bases
ATGCTGGTCTTTATTGGACTGGGTAATCCTGGCCCACGTTATGTTCTCACACGGCATAACGTGGGCTTTCTCTTCGTGGA
CGAGTTACTGAAGAAATTTCAAATTGGAAGTAGTTACAGAGCAGAAACCTATGAAGCTTTCAAATTAAAGTCACAATCAC
CTGCAATCGCTGTCAAGCCTCTCACCTATATGAACAACAGCGGGATCGCCGTAAGGGATCTAATCCGCGATTTTGGATTG
TCTCAGAACGACAGGCTTATCGTAATTTATGATGATGTCTGGATACCACTCGGCCGCCTGAGGATCAGGGAACGCGGAAG
TGACGGGGGACACAACGGGGTAAAATCAATCATATCTTCTCTTGGAACCCAGGAATTTTCCAGAATCAGGATTGGAATCG
GTCCCAAGCCTGATGACATTGACATGGTTTCCTATGTTCTCGGGGAATTTACAGATTCCGAATTAAAGGTATTGTGGAAA
GTTCTTGATCTCGCGATATCAGCCGCTCAAGAGATGTTCGCTGCGGATTTTAAGAAAGTCATGTCCCGCTACAACTCTAT
AGGGGTGGAGTAG

Upstream 100 bases:

>100_bases
ACCACGCTCTATGCTGGCAGAGGGAACCGCTGCCGAGAAGGAAGAGGAAGCGGAACCTGAAGTCATTGAAAAGGGAAAGA
AAGAGGAAGAAGAGGAATAA

Downstream 100 bases:

>100_bases
ACTTATGAGGTTCAACCTGAAAGCTCCCTTTGAACCAAGGGGGGATCAACCCGAGGCGATAACCAGGTTGGTGGAAGGTT
TAAGGCGAGGAGACAGGTTT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MLVFIGLGNPGPRYVLTRHNVGFLFVDELLKKFQIGSSYRAETYEAFKLKSQSPAIAVKPLTYMNNSGIAVRDLIRDFGL
SQNDRLIVIYDDVWIPLGRLRIRERGSDGGHNGVKSIISSLGTQEFSRIRIGIGPKPDDIDMVSYVLGEFTDSELKVLWK
VLDLAISAAQEMFAADFKKVMSRYNSIGVE

Sequences:

>Translated_190_residues
MLVFIGLGNPGPRYVLTRHNVGFLFVDELLKKFQIGSSYRAETYEAFKLKSQSPAIAVKPLTYMNNSGIAVRDLIRDFGL
SQNDRLIVIYDDVWIPLGRLRIRERGSDGGHNGVKSIISSLGTQEFSRIRIGIGPKPDDIDMVSYVLGEFTDSELKVLWK
VLDLAISAAQEMFAADFKKVMSRYNSIGVE
>Mature_190_residues
MLVFIGLGNPGPRYVLTRHNVGFLFVDELLKKFQIGSSYRAETYEAFKLKSQSPAIAVKPLTYMNNSGIAVRDLIRDFGL
SQNDRLIVIYDDVWIPLGRLRIRERGSDGGHNGVKSIISSLGTQEFSRIRIGIGPKPDDIDMVSYVLGEFTDSELKVLWK
VLDLAISAAQEMFAADFKKVMSRYNSIGVE

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=168, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1787455, Length=197, Percent_Identity=32.994923857868, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321983, Length=185, Percent_Identity=30.8108108108108, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_KOSOT (C5CFR9)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941421.1
- GeneID:   7968132
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1731
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21293; Mature: 21293

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVFIGLGNPGPRYVLTRHNVGFLFVDELLKKFQIGSSYRAETYEAFKLKSQSPAIAVKP
CEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHEECCCCCCEEEEE
LTYMNNSGIAVRDLIRDFGLSQNDRLIVIYDDVWIPLGRLRIRERGSDGGHNGVKSIISS
EEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHEECCCCCCCCHHHHHHHHH
LGTQEFSRIRIGIGPKPDDIDMVSYVLGEFTDSELKVLWKVLDLAISAAQEMFAADFKKV
HCCHHHHEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSRYNSIGVE
HHHHHHCCCC
>Mature Secondary Structure
MLVFIGLGNPGPRYVLTRHNVGFLFVDELLKKFQIGSSYRAETYEAFKLKSQSPAIAVKP
CEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHEECCCCCCEEEEE
LTYMNNSGIAVRDLIRDFGLSQNDRLIVIYDDVWIPLGRLRIRERGSDGGHNGVKSIISS
EEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHEECCCCCCCCHHHHHHHHH
LGTQEFSRIRIGIGPKPDDIDMVSYVLGEFTDSELKVLWKVLDLAISAAQEMFAADFKKV
HCCHHHHEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSRYNSIGVE
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA