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The map label for this gene is punA [H]

Identifier: 239617848

GI number: 239617848

Start: 1572785

End: 1573591

Strand: Reverse

Name: punA [H]

Synonym: Kole_1474

Alternate gene names: 239617848

Gene position: 1573591-1572785 (Counterclockwise)

Preceding gene: 239617854

Following gene: 239617847

Centisome position: 68.35

GC content: 40.64

Gene sequence:

>807_bases
ATGAAAGACTACAAAAAACTTGCAAAACACCTTCTTGAACGCACAAACGTAAAGCCTGAATTCGCTCTGATTCTTGGATC
TGGATTGGGATATCTAACAAAATATTTTGAAGATTCCCATGCTATTTCATATTCCGAACTGGAAGGTTTTCCTCAAAGTA
CTGTTGAAGGACACGCAGGAAGATTCGTTCTCGGAAAATTCCATGGAAAAAACGTTATAGCCATGGACGGTCGTTTCCAC
TATTATGAAGGCCACGAAATAAAAGATGTTGTGACACCGATCTATGTTTTCAAAGAAATGGGAATTTCGCGTCTCATAAT
AACCAATGCCGCAGGTGGTATAAATCGTTCCTTCGAACCCGGAGATATAGTGGCAATAACAGATATTATAAATTTCGCTT
TCAGAAATCCACTCATCGGCCCTAATGACGAAAGATACGGTGTCAGATTTCCTGATATGTCGAAAACAATCGACTTCGAT
TGGCTTGAAAAAGTCGAAAAAACTGCTGAATCCAGGGGAATAAAACTAAAACGCGGAACATATGTGTGGGTTACCGGTCC
ATCCTACGAAACACCAGCCGAGATAAAGGCTTACGAATTTTCGGGAGCTGACATGGTTGGAATGTCAACAGTTCCTGAGA
TAATTGCCGCGAGACATTGCGGTATAAATCTCATAGCTTTTTCTTGCATAACGAACATGGCTTCAGGTATATTAGACAAA
AAACTCGTCCATGAAGAGGTTGTCCAGACAGCTCAAAGAGTCAAAAGCGCTTTCAGCAAAGTCGTAGGCATAGCTTTAGA
AGTTTAA

Upstream 100 bases:

>100_bases
ATTCTACCACGGCTTGTTATATAATCAATAATGCCTACCAAAAAACTTATGTTTGGGTATATACTTATGATTAAAGATTT
TTTAATATGGAGGAATGAGT

Downstream 100 bases:

>100_bases
AGGAGGATGAATAATGCGAACAATGAATTCACTCATGTCTGAAATCGAACAAGCAAAAAAAATCGTTGTATGCGGTCATA
TCATGCCTGATGGTGATTGT

Product: purine nucleoside phosphorylase I, inosine and guanosine-specific

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKDYKKLAKHLLERTNVKPEFALILGSGLGYLTKYFEDSHAISYSELEGFPQSTVEGHAGRFVLGKFHGKNVIAMDGRFH
YYEGHEIKDVVTPIYVFKEMGISRLIITNAAGGINRSFEPGDIVAITDIINFAFRNPLIGPNDERYGVRFPDMSKTIDFD
WLEKVEKTAESRGIKLKRGTYVWVTGPSYETPAEIKAYEFSGADMVGMSTVPEIIAARHCGINLIAFSCITNMASGILDK
KLVHEEVVQTAQRVKSAFSKVVGIALEV

Sequences:

>Translated_268_residues
MKDYKKLAKHLLERTNVKPEFALILGSGLGYLTKYFEDSHAISYSELEGFPQSTVEGHAGRFVLGKFHGKNVIAMDGRFH
YYEGHEIKDVVTPIYVFKEMGISRLIITNAAGGINRSFEPGDIVAITDIINFAFRNPLIGPNDERYGVRFPDMSKTIDFD
WLEKVEKTAESRGIKLKRGTYVWVTGPSYETPAEIKAYEFSGADMVGMSTVPEIIAARHCGINLIAFSCITNMASGILDK
KLVHEEVVQTAQRVKSAFSKVVGIALEV
>Mature_268_residues
MKDYKKLAKHLLERTNVKPEFALILGSGLGYLTKYFEDSHAISYSELEGFPQSTVEGHAGRFVLGKFHGKNVIAMDGRFH
YYEGHEIKDVVTPIYVFKEMGISRLIITNAAGGINRSFEPGDIVAITDIINFAFRNPLIGPNDERYGVRFPDMSKTIDFD
WLEKVEKTAESRGIKLKRGTYVWVTGPSYETPAEIKAYEFSGADMVGMSTVPEIIAARHCGINLIAFSCITNMASGILDK
KLVHEEVVQTAQRVKSAFSKVVGIALEV

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=274, Percent_Identity=45.6204379562044, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI47132622, Length=222, Percent_Identity=28.8288288288288, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788746, Length=249, Percent_Identity=42.1686746987952, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17541190, Length=251, Percent_Identity=37.4501992031873, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323238, Length=237, Percent_Identity=41.7721518987342, Blast_Score=169, Evalue=4e-43,
Organism=Drosophila melanogaster, GI45552887, Length=267, Percent_Identity=42.3220973782771, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45552885, Length=267, Percent_Identity=42.3220973782771, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24656093, Length=267, Percent_Identity=42.3220973782771, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24656090, Length=267, Percent_Identity=42.3220973782771, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24762376, Length=239, Percent_Identity=31.7991631799163, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI221459247, Length=175, Percent_Identity=27.4285714285714, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29849; Mature: 29849

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDYKKLAKHLLERTNVKPEFALILGSGLGYLTKYFEDSHAISYSELEGFPQSTVEGHAG
CCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCCCCHHHHCCCCCHHHCCCCC
RFVLGKFHGKNVIAMDGRFHYYEGHEIKDVVTPIYVFKEMGISRLIITNAAGGINRSFEP
EEEEEEECCCEEEEECCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
GDIVAITDIINFAFRNPLIGPNDERYGVRFPDMSKTIDFDWLEKVEKTAESRGIKLKRGT
CCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCE
YVWVTGPSYETPAEIKAYEFSGADMVGMSTVPEIIAARHCGINLIAFSCITNMASGILDK
EEEEECCCCCCCCCEEEEEECCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KLVHEEVVQTAQRVKSAFSKVVGIALEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDYKKLAKHLLERTNVKPEFALILGSGLGYLTKYFEDSHAISYSELEGFPQSTVEGHAG
CCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCCCCHHHHCCCCCHHHCCCCC
RFVLGKFHGKNVIAMDGRFHYYEGHEIKDVVTPIYVFKEMGISRLIITNAAGGINRSFEP
EEEEEEECCCEEEEECCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
GDIVAITDIINFAFRNPLIGPNDERYGVRFPDMSKTIDFDWLEKVEKTAESRGIKLKRGT
CCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCE
YVWVTGPSYETPAEIKAYEFSGADMVGMSTVPEIIAARHCGINLIAFSCITNMASGILDK
EEEEECCCCCCCCCEEEEEECCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KLVHEEVVQTAQRVKSAFSKVVGIALEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]