Click here to switch to the map view.

The map label for this gene is rpfG [H]

Identifier: 239616991

GI number: 239616991

Start: 638026

End: 639678

Strand: Reverse

Name: rpfG [H]

Synonym: Kole_0590

Alternate gene names: 239616991

Gene position: 639678-638026 (Counterclockwise)

Preceding gene: 239616993

Following gene: 239616985

Centisome position: 27.79

GC content: 39.99

Gene sequence:

>1653_bases
ATGAAAAAGGACGTTGAGGAGTTACTTTTAGGTTTAGAAATTTCTGAGAAAAGTATTCCTAAAATATGTGAGATTCTTAA
AAAGATTGAAGATAAAAAAGAGCGAGATTACCTTGACGCTTTGAAAAAACTTTGTACCACTGCAGAGGGAAGGGATACTC
TCAGTAGCTATGATTTAGAATGGTTTAGAAAACTTGCTGCTTTCAATGCGAAACGTGGAAAGTTGACAAGAGCCTATGAT
ATTGTGAAGAGAGTTATGGAAATAGCCGAAAAGTTTCCTGAAAACAGCCGGGAGAAGATTTCTTTCAGGCTTTTTTATAT
GATGATCAGTTACGAGCTCAATAAAAGAACGGAGGTCATAAAACAGGCGAAGGAATTACTCGGCATAGAAAAGTTTGTAC
CGGAGGACCTCAGAGCTACGTATTATAATGATCTTGGTCTGGTGGCATCTTATTTTGAAATCGGAGAAGACCCTGTTGAA
CTTCTTGAAAAGGCAATGAAGTACCCGGAAGATTTTGCCGGTTCATTGCTTGCTCCCTACAACCTTACGGAGTATTACTA
TTATCGTGGTGACTTTGAAAAAGCGCTTAACCTTGTTACCTCTCTTAAAAAGAAGGCCTTTGACTATCTTCTTTCCAAAT
GTGCTGTTATGGAGTTAAAATGTTTTCTTGCTCTCTCTGAGATAGAAAAAGCTGAGAATGTCGTTAAAGAACTCGAAAAA
ATGAACTCTGAGAATCCTGAGCGTTTGGATATGGGAATATCTCTGGTCTTTCTCGGTTATTACTATGTTAGTCGGGGAGA
TATTGACAAAGCCAAAGAGTATGTTCGGTGGATAGATCGCATACTGGAGAGAGAATACACTCCTTATCTCTATGGAGAGA
GTCAGGCGCTAAAAGCTGAGATCCTTAACGCTCAAGGAGAGAGCTTTAAGGCGATAGAGGTAATGATAGAAGCTTTCGAA
ATACTTCGGAAAGAGAAGATATTATCTCCCCACGTTAGAAAATATCTTAAATCCGCTTTGTTCAGACTATGGGACATTTT
CAAGAAATTGGTAAATGAGTTGAGACAAAGAGATGATTATACAGCCAGCCATACTCTCAGAGTTTCGTATTTTGCTTACT
CGTTAGCGCGTCATATGAATTATTCTACAACCGATTTGTTCTATCTGATAATTGGCGGAATGCTTCACGATTATGGGAAG
ATTAAAGTTCCTCTTGAAATACTCAACAAGAAAGGAAAGCTTGACCCTGAAGAATTGAAAGCGATACGGAAGCACCCGGT
TTATGGGGCGGAATTCCTTGAAGGGTTGAAATTTCCGGATTTAATACGCCACGTAGTTTTGATGCATCATGAGCGGATAG
ATGGCAAAGGATACCCACTTGGCCTGAAGAATGGGGAGATACCTGAAGTTGCTCAGATAGTGGCCATTGCCGACATTTTT
GATGCTCTGACCACAGATAGGCCTTACAGGAAGGCGTTATCCAAAGAAGAGGCTGTAAGGTATCTATTGAGCCTTCGCGG
GCAACTGGTTGAAGATCACTTGCTGAATAATTTTGCTAAGATGGCGGAGATGATCCAACCAAGGAACTATCGTATCGCTT
TTGAAGAAATCTGGAGAGAAACGATAGAAGATTTGTTTGAAGTTGTAGAATGA

Upstream 100 bases:

>100_bases
GACAAGGTTTTTTGTGAGTCTTTCTCAGACTTTATAACAGCTTTGAATGATATAATTAAGCAAGAAATTAACTGTTCAAC
CGCAGATTCTGGAGGTTTTT

Downstream 100 bases:

>100_bases
AGACAGGGCTCCATATCATTGGAGCCCTGTCTTCTATATAGTATTAAAGATTAATCTTCGCTAGGATCGAGCCATGCTCC
GGGCATTCTATCTGAGAGTG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKKDVEELLLGLEISEKSIPKICEILKKIEDKKERDYLDALKKLCTTAEGRDTLSSYDLEWFRKLAAFNAKRGKLTRAYD
IVKRVMEIAEKFPENSREKISFRLFYMMISYELNKRTEVIKQAKELLGIEKFVPEDLRATYYNDLGLVASYFEIGEDPVE
LLEKAMKYPEDFAGSLLAPYNLTEYYYYRGDFEKALNLVTSLKKKAFDYLLSKCAVMELKCFLALSEIEKAENVVKELEK
MNSENPERLDMGISLVFLGYYYVSRGDIDKAKEYVRWIDRILEREYTPYLYGESQALKAEILNAQGESFKAIEVMIEAFE
ILRKEKILSPHVRKYLKSALFRLWDIFKKLVNELRQRDDYTASHTLRVSYFAYSLARHMNYSTTDLFYLIIGGMLHDYGK
IKVPLEILNKKGKLDPEELKAIRKHPVYGAEFLEGLKFPDLIRHVVLMHHERIDGKGYPLGLKNGEIPEVAQIVAIADIF
DALTTDRPYRKALSKEEAVRYLLSLRGQLVEDHLLNNFAKMAEMIQPRNYRIAFEEIWRETIEDLFEVVE

Sequences:

>Translated_550_residues
MKKDVEELLLGLEISEKSIPKICEILKKIEDKKERDYLDALKKLCTTAEGRDTLSSYDLEWFRKLAAFNAKRGKLTRAYD
IVKRVMEIAEKFPENSREKISFRLFYMMISYELNKRTEVIKQAKELLGIEKFVPEDLRATYYNDLGLVASYFEIGEDPVE
LLEKAMKYPEDFAGSLLAPYNLTEYYYYRGDFEKALNLVTSLKKKAFDYLLSKCAVMELKCFLALSEIEKAENVVKELEK
MNSENPERLDMGISLVFLGYYYVSRGDIDKAKEYVRWIDRILEREYTPYLYGESQALKAEILNAQGESFKAIEVMIEAFE
ILRKEKILSPHVRKYLKSALFRLWDIFKKLVNELRQRDDYTASHTLRVSYFAYSLARHMNYSTTDLFYLIIGGMLHDYGK
IKVPLEILNKKGKLDPEELKAIRKHPVYGAEFLEGLKFPDLIRHVVLMHHERIDGKGYPLGLKNGEIPEVAQIVAIADIF
DALTTDRPYRKALSKEEAVRYLLSLRGQLVEDHLLNNFAKMAEMIQPRNYRIAFEEIWRETIEDLFEVVE
>Mature_550_residues
MKKDVEELLLGLEISEKSIPKICEILKKIEDKKERDYLDALKKLCTTAEGRDTLSSYDLEWFRKLAAFNAKRGKLTRAYD
IVKRVMEIAEKFPENSREKISFRLFYMMISYELNKRTEVIKQAKELLGIEKFVPEDLRATYYNDLGLVASYFEIGEDPVE
LLEKAMKYPEDFAGSLLAPYNLTEYYYYRGDFEKALNLVTSLKKKAFDYLLSKCAVMELKCFLALSEIEKAENVVKELEK
MNSENPERLDMGISLVFLGYYYVSRGDIDKAKEYVRWIDRILEREYTPYLYGESQALKAEILNAQGESFKAIEVMIEAFE
ILRKEKILSPHVRKYLKSALFRLWDIFKKLVNELRQRDDYTASHTLRVSYFAYSLARHMNYSTTDLFYLIIGGMLHDYGK
IKVPLEILNKKGKLDPEELKAIRKHPVYGAEFLEGLKFPDLIRHVVLMHHERIDGKGYPLGLKNGEIPEVAQIVAIADIF
DALTTDRPYRKALSKEEAVRYLLSLRGQLVEDHLLNNFAKMAEMIQPRNYRIAFEEIWRETIEDLFEVVE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 64395; Mature: 64395

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDVEELLLGLEISEKSIPKICEILKKIEDKKERDYLDALKKLCTTAEGRDTLSSYDLE
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHH
WFRKLAAFNAKRGKLTRAYDIVKRVMEIAEKFPENSREKISFRLFYMMISYELNKRTEVI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQAKELLGIEKFVPEDLRATYYNDLGLVASYFEIGEDPVELLEKAMKYPEDFAGSLLAPY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCC
NLTEYYYYRGDFEKALNLVTSLKKKAFDYLLSKCAVMELKCFLALSEIEKAENVVKELEK
CCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNSENPERLDMGISLVFLGYYYVSRGDIDKAKEYVRWIDRILEREYTPYLYGESQALKAE
HCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHH
ILNAQGESFKAIEVMIEAFEILRKEKILSPHVRKYLKSALFRLWDIFKKLVNELRQRDDY
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TASHTLRVSYFAYSLARHMNYSTTDLFYLIIGGMLHDYGKIKVPLEILNKKGKLDPEELK
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCHHHHH
AIRKHPVYGAEFLEGLKFPDLIRHVVLMHHERIDGKGYPLGLKNGEIPEVAQIVAIADIF
HHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
DALTTDRPYRKALSKEEAVRYLLSLRGQLVEDHLLNNFAKMAEMIQPRNYRIAFEEIWRE
HHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
TIEDLFEVVE
HHHHHHHHHC
>Mature Secondary Structure
MKKDVEELLLGLEISEKSIPKICEILKKIEDKKERDYLDALKKLCTTAEGRDTLSSYDLE
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHH
WFRKLAAFNAKRGKLTRAYDIVKRVMEIAEKFPENSREKISFRLFYMMISYELNKRTEVI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQAKELLGIEKFVPEDLRATYYNDLGLVASYFEIGEDPVELLEKAMKYPEDFAGSLLAPY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCC
NLTEYYYYRGDFEKALNLVTSLKKKAFDYLLSKCAVMELKCFLALSEIEKAENVVKELEK
CCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNSENPERLDMGISLVFLGYYYVSRGDIDKAKEYVRWIDRILEREYTPYLYGESQALKAE
HCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHH
ILNAQGESFKAIEVMIEAFEILRKEKILSPHVRKYLKSALFRLWDIFKKLVNELRQRDDY
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TASHTLRVSYFAYSLARHMNYSTTDLFYLIIGGMLHDYGKIKVPLEILNKKGKLDPEELK
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCHHHHH
AIRKHPVYGAEFLEGLKFPDLIRHVVLMHHERIDGKGYPLGLKNGEIPEVAQIVAIADIF
HHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
DALTTDRPYRKALSKEEAVRYLLSLRGQLVEDHLLNNFAKMAEMIQPRNYRIAFEEIWRE
HHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
TIEDLFEVVE
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]