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The map label for this gene is cooH [H]

Identifier: 239616969

GI number: 239616969

Start: 618398

End: 619606

Strand: Reverse

Name: cooH [H]

Synonym: Kole_0568

Alternate gene names: 239616969

Gene position: 619606-618398 (Counterclockwise)

Preceding gene: 239616970

Following gene: 239616968

Centisome position: 26.91

GC content: 43.42

Gene sequence:

>1209_bases
ATGAGTAAAGAATTTAATCTACCGGTTGGACCCTTTCATCCGTTACTCGAAGAAGCAGAATATTTCAAAATAAAACTCGA
CGGAGAGCGGGTAGTCGACATTGAGATGGAAACCGGATGGATGCACAGGGGTATAGAAAAAATCTCTGAAGAAAGCACCT
TTGACCAATCTATATTTGTGGTGGAAAGAATTTGCGGTATATGCTCCGCTTCCCATCCCTTTGCCTGTATAAACGCTTTT
GAGGATATTGGAGAGATTGAAGTCCCACTTAAAGCCCGCTATATAAGATCTCTTATTGGTGAGTTAGAAAGGGTTCACAG
TCATCTTCTGTGGTTGGGACTCGCGGGACATTTCATTGGTTACGATACTTTGTGGATGTGGGCATGGAAATACAGGGAGC
CGGTACTTGAGGTTATTGAGATGATAACCGGGAACAGAAACCATTATGGGACGTTCAAAATCGGTGGTGTGAGAAGAGAT
TACGATGTTTCTTTGAATTCTGTTGTTCATGAAAAACTTGACTTTCTCGAGAAAAAACTAACTATGGTTGCCAAAGCCGT
AGCGAATGATCCGGTAGTAAGGAGCAGATTGTGTGGAGTTGGAGTACTCACCAAAGAAGAGGCAATAAGCTATTGCGCTG
TTGGTCCCACCGCGAGAGCTTCTGGTGTTGCTATCGATGTCAGGAAAGATGAACCTTACGCGGCCTATGATCTGCTCGAT
TTTGGGGTTGTTGTAAGGGAAGAGGGCGATATTTTTGCCAAAACAGTGGTACGGATTTTAGAGGTATTTGAAAGCATAAA
GATGATCAGGCAAATAATAGAGAAGTTGAGGAAGGTTAAAGGGCCGATATACAATGACGTTAAGGAAATACCTGCCGGCG
AGGGGATAGGACGGCACGAAGCTCCAAGAGGAGAGGTTTTCCACTATATAAGAACGGATGGTTCTAACCGCCCTGTCAGG
CACAAAATAAGAGCACCAAGTTACGTTAATATCCCGACTTACAAAGCTTCCTGTATCGGCGAAAATCTTGCCGACGTATT
GATTACCCTCGCTTCTGTTGATCCCTGTTATTGCTGCACCGAAAGATCCGTCAGACTTCAGACCGAAAAAGGGATGGAAA
TAACCAGCAACCTGTTGAAACTATCCCAGGATAAGACTGATAGACTCATTAAAAACATGGGAGAGTATAAATTATGGAAA
TCCTTATGA

Upstream 100 bases:

>100_bases
TTTGGTGTTGGGTTTATAGACCATCCCGGATTGAAACCGCTTCTGACGGAGAATAATGAGAAGATACCAAAAAAACCTTT
GAGAGTGGAGAGAAGAAAAC

Downstream 100 bases:

>100_bases
CTCCGATTTCTTTTATGCTTTTTTTGCCGTTAGTTGTGATTCTATTAGATCTTGTTTTTCCGGTAAAAATCGTGCGGTGG
CTTAACATAATAACTATCGC

Product: NADH-ubiquinone oxidoreductase chain 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSKEFNLPVGPFHPLLEEAEYFKIKLDGERVVDIEMETGWMHRGIEKISEESTFDQSIFVVERICGICSASHPFACINAF
EDIGEIEVPLKARYIRSLIGELERVHSHLLWLGLAGHFIGYDTLWMWAWKYREPVLEVIEMITGNRNHYGTFKIGGVRRD
YDVSLNSVVHEKLDFLEKKLTMVAKAVANDPVVRSRLCGVGVLTKEEAISYCAVGPTARASGVAIDVRKDEPYAAYDLLD
FGVVVREEGDIFAKTVVRILEVFESIKMIRQIIEKLRKVKGPIYNDVKEIPAGEGIGRHEAPRGEVFHYIRTDGSNRPVR
HKIRAPSYVNIPTYKASCIGENLADVLITLASVDPCYCCTERSVRLQTEKGMEITSNLLKLSQDKTDRLIKNMGEYKLWK
SL

Sequences:

>Translated_402_residues
MSKEFNLPVGPFHPLLEEAEYFKIKLDGERVVDIEMETGWMHRGIEKISEESTFDQSIFVVERICGICSASHPFACINAF
EDIGEIEVPLKARYIRSLIGELERVHSHLLWLGLAGHFIGYDTLWMWAWKYREPVLEVIEMITGNRNHYGTFKIGGVRRD
YDVSLNSVVHEKLDFLEKKLTMVAKAVANDPVVRSRLCGVGVLTKEEAISYCAVGPTARASGVAIDVRKDEPYAAYDLLD
FGVVVREEGDIFAKTVVRILEVFESIKMIRQIIEKLRKVKGPIYNDVKEIPAGEGIGRHEAPRGEVFHYIRTDGSNRPVR
HKIRAPSYVNIPTYKASCIGENLADVLITLASVDPCYCCTERSVRLQTEKGMEITSNLLKLSQDKTDRLIKNMGEYKLWK
SL
>Mature_401_residues
SKEFNLPVGPFHPLLEEAEYFKIKLDGERVVDIEMETGWMHRGIEKISEESTFDQSIFVVERICGICSASHPFACINAFE
DIGEIEVPLKARYIRSLIGELERVHSHLLWLGLAGHFIGYDTLWMWAWKYREPVLEVIEMITGNRNHYGTFKIGGVRRDY
DVSLNSVVHEKLDFLEKKLTMVAKAVANDPVVRSRLCGVGVLTKEEAISYCAVGPTARASGVAIDVRKDEPYAAYDLLDF
GVVVREEGDIFAKTVVRILEVFESIKMIRQIIEKLRKVKGPIYNDVKEIPAGEGIGRHEAPRGEVFHYIRTDGSNRPVRH
KIRAPSYVNIPTYKASCIGENLADVLITLASVDPCYCCTERSVRLQTEKGMEITSNLLKLSQDKTDRLIKNMGEYKLWKS
L

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=386, Percent_Identity=28.4974093264249, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI260898743, Length=376, Percent_Identity=28.7234042553192, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1789076, Length=366, Percent_Identity=33.879781420765, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1788832, Length=384, Percent_Identity=30.9895833333333, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI145693162, Length=387, Percent_Identity=28.6821705426357, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17568379, Length=389, Percent_Identity=27.2493573264781, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17555284, Length=382, Percent_Identity=26.4397905759162, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI221459469, Length=371, Percent_Identity=30.188679245283, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24638644, Length=386, Percent_Identity=28.2383419689119, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 45627; Mature: 45496

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEFNLPVGPFHPLLEEAEYFKIKLDGERVVDIEMETGWMHRGIEKISEESTFDQSIFV
CCCCCCCCCCCCHHHHCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHH
VERICGICSASHPFACINAFEDIGEIEVPLKARYIRSLIGELERVHSHLLWLGLAGHFIG
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDTLWMWAWKYREPVLEVIEMITGNRNHYGTFKIGGVRRDYDVSLNSVVHEKLDFLEKKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
TMVAKAVANDPVVRSRLCGVGVLTKEEAISYCAVGPTARASGVAIDVRKDEPYAAYDLLD
HHHHHHHCCCCHHHHHHHCCCCEEHHHHHHECCCCCCCCCCCEEEEEECCCCCHHHHHHH
FGVVVREEGDIFAKTVVRILEVFESIKMIRQIIEKLRKVKGPIYNDVKEIPAGEGIGRHE
HCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCC
APRGEVFHYIRTDGSNRPVRHKIRAPSYVNIPTYKASCIGENLADVLITLASVDPCYCCT
CCCCCEEEEEECCCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEC
ERSVRLQTEKGMEITSNLLKLSQDKTDRLIKNMGEYKLWKSL
CCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SKEFNLPVGPFHPLLEEAEYFKIKLDGERVVDIEMETGWMHRGIEKISEESTFDQSIFV
CCCCCCCCCCCHHHHCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHH
VERICGICSASHPFACINAFEDIGEIEVPLKARYIRSLIGELERVHSHLLWLGLAGHFIG
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDTLWMWAWKYREPVLEVIEMITGNRNHYGTFKIGGVRRDYDVSLNSVVHEKLDFLEKKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
TMVAKAVANDPVVRSRLCGVGVLTKEEAISYCAVGPTARASGVAIDVRKDEPYAAYDLLD
HHHHHHHCCCCHHHHHHHCCCCEEHHHHHHECCCCCCCCCCCEEEEEECCCCCHHHHHHH
FGVVVREEGDIFAKTVVRILEVFESIKMIRQIIEKLRKVKGPIYNDVKEIPAGEGIGRHE
HCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCC
APRGEVFHYIRTDGSNRPVRHKIRAPSYVNIPTYKASCIGENLADVLITLASVDPCYCCT
CCCCCEEEEEECCCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEC
ERSVRLQTEKGMEITSNLLKLSQDKTDRLIKNMGEYKLWKSL
CCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]