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The map label for this gene is hypF [H]

Identifier: 239616961

GI number: 239616961

Start: 609324

End: 611567

Strand: Reverse

Name: hypF [H]

Synonym: Kole_0559

Alternate gene names: 239616961

Gene position: 611567-609324 (Counterclockwise)

Preceding gene: 239616962

Following gene: 239616960

Centisome position: 26.57

GC content: 41.93

Gene sequence:

>2244_bases
ATGCAGGAAATAGAGGTGTTTGAAATAATGATCAACGGTATCGTTCAAGGCGTTGGTTTTAGGCCTTTCGTGGCAACCCT
GGCTGAAGACCTCGGGTTACACGGTTATGTGGAGAACTGCGAAAGTGGCGTAAGAATCGTTCTTGATTGCGATGAAACGA
AGTTGAGAGAATTCATCGAAATTTTGAATGAAAGAAAGCCAATGCCAGCGAAAATTAGAGAGCTGTCCTTTTCAAAAAAA
GCTGTGAAAACAAACAAGATTAGGGGTTTCAGAATAATCAACAGTAAGAAGACGGGAAAAATCAACACATCGATACCACC
GGACATAGCCATGTGCGACAAATGCATTGGTGAGATGTTTTCAAAAGGGAACAGAAGATATCTTTATCCCTTTACCAACT
GTGCCCGATGTGGACCACGTTTTTCAATAGCTTATGTGCTGCCTTACGACAGAGACAAAACATCAATGGATGACTTTTCC
ATGTGCCATGACTGTAATACCGAATACACAAACCTGAGAGATTGGCGATACCACGCTCAAACCAATTGTTGCGAAGATTG
TGGACCAAAATACTGGATCGTAGAAATTATCAAAAACGAGATTGTAAGCCACTTTTCAGGTGAAGAAGCTGTTAAGCAGG
CGATAGGCATAATACGTGCCGGTGGCACCGTGGCTATAAAAGGAATAGGAGGGATTCACCTCGTTTGTAAACCAGACCCG
GATGCTATCAAAAAACTGCGGAATTTGAAAGACAGACCCAGGAAACCCTTTGCGATTATGGCAAAAGATCTGGAGACAGT
TAAAAAATTTGCATATGTTGGGGAGGTCGAGGAAAGGCTGCTTCTTTCTCCTGCAAGGCCAATCGTTCTTTTAAGAAAGC
GTGGAGACATTTTCTACGATGTTTCGCCGGGATTTCATACCATAGGAGTGATGCTTCCCTATACCGGTATCCATTACCTT
CTTCTGGAAGATTTTTTTGTGCTGGTGATGACGAGCGGAAATATATCCGGAGAGATGCTGTGTGCCGATAATTTTGAGGC
TCTTACAAAACTTTCGAAACTAAGTGATGGTCTTTTGCTGCATGACAGAAAAATTGTAAACAGATGCGACGATTCCGTTA
TTAAGGTTTTAGCAGGAAAGCCGGTTTTTGTAAGAAAATCCCGTGGTTTTATTCCGGAGGTCATAAAGATACCGTTAAGA
AACAGAAAGCACCCGGAAATTCTCGCCACAGGAGCGGACCTAAAATCCAGTTTCGGTTTTTTCAGAGAAGAGAGTTTTTA
TGGGAGCCAGTATCTTGGGGATCTTTCGTATTACATAAATCAGGTGCAGTATCAAAAAATGCTAAAGCGCTTCGAAAAGC
TTTTCGATTTACACGCTAGGGTTGTAGTTACCGATCTTCATCCAGATTACTTTTCGAAAACGCTTGGTGAAGAATACGCT
GAAAACAGGGATTGTGAAATATATTCCTGTCAACACCATGTTGCCCATATTTACAGCGTTATGGCTGAGAAAGCATTAAA
GAAATGTATAGGTGTTGCTTTTGACGGTACCGGATACGGATTTGATGGGAAAATCTGGGGAGGAGAGTTTTTCTCTATTG
ATAATTCGAAATGTATGAGATTTGCACATTTACGTTACATCCCAATGCCCTCAGGAGAGCTGGCTATTGAAAAACCGGAG
CTCATGGCTTATGCTTACCTCATTGATTCCGGGGTGGATCTGAAAGATGAAAAAGCTCACCTGTTGTATGAAAACGCTCC
CGTGAAGACTTCTTCAGCTGGAAGGTTGTTCGATGCTGCCGCTGCTGTACTCGATGTTTGCAAAGAGAATACCTTTGAAG
GGGAAGCCCCAATGAAGCTGGAATCTGTTGCCAAAAGGCATTCTGAATCATTTTCCTGGGTATTGGATAAATCTGCCTTA
CCATGGCAAATTGACTTTCGGAAAATGATAAGGGAACTTTATAGCGAAAAAGATAAAAATTCAATCTCAGAACTCGCTTC
GAAATTCCATCGAACGCTCGCCGAAGCTATTTCTGTTGTGTGTGGAGAAATAAAGAAACTTACAGGAGAAAAGAACGTAT
GCCTTTCTGGAGGTGTTTTTCAAAACAAACTTTTGCTTGAGGTGGCCGTTGCCCTGTTAGAGAAAAAAGGTTTTAATGTT
TGGTTTAATGAAAAAGTCTCTCCAAACGATGAGGGAATAGCGTTTGGGCAGATCTACTGGCATGTGATTGGAAGTGATGG
ATGA

Upstream 100 bases:

>100_bases
ACCTGGTTGGATTAAGGCCCGCCGTCCTTTCTCTTGGCACGACCCTTTCTCTGGAAATGAAAAGGATTATCGAGACCATT
GTGAAAGGATTTGAAAGAGA

Downstream 100 bases:

>100_bases
TGTGCTTGGCAATACCCTTGAAGGTTATCAAAATCAAAGATAATAAAGCCCTTGTAGGACGGGAAGGAATAAAACTTGAG
GTTGACATAAGACTTGTCCC

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MQEIEVFEIMINGIVQGVGFRPFVATLAEDLGLHGYVENCESGVRIVLDCDETKLREFIEILNERKPMPAKIRELSFSKK
AVKTNKIRGFRIINSKKTGKINTSIPPDIAMCDKCIGEMFSKGNRRYLYPFTNCARCGPRFSIAYVLPYDRDKTSMDDFS
MCHDCNTEYTNLRDWRYHAQTNCCEDCGPKYWIVEIIKNEIVSHFSGEEAVKQAIGIIRAGGTVAIKGIGGIHLVCKPDP
DAIKKLRNLKDRPRKPFAIMAKDLETVKKFAYVGEVEERLLLSPARPIVLLRKRGDIFYDVSPGFHTIGVMLPYTGIHYL
LLEDFFVLVMTSGNISGEMLCADNFEALTKLSKLSDGLLLHDRKIVNRCDDSVIKVLAGKPVFVRKSRGFIPEVIKIPLR
NRKHPEILATGADLKSSFGFFREESFYGSQYLGDLSYYINQVQYQKMLKRFEKLFDLHARVVVTDLHPDYFSKTLGEEYA
ENRDCEIYSCQHHVAHIYSVMAEKALKKCIGVAFDGTGYGFDGKIWGGEFFSIDNSKCMRFAHLRYIPMPSGELAIEKPE
LMAYAYLIDSGVDLKDEKAHLLYENAPVKTSSAGRLFDAAAAVLDVCKENTFEGEAPMKLESVAKRHSESFSWVLDKSAL
PWQIDFRKMIRELYSEKDKNSISELASKFHRTLAEAISVVCGEIKKLTGEKNVCLSGGVFQNKLLLEVAVALLEKKGFNV
WFNEKVSPNDEGIAFGQIYWHVIGSDG

Sequences:

>Translated_747_residues
MQEIEVFEIMINGIVQGVGFRPFVATLAEDLGLHGYVENCESGVRIVLDCDETKLREFIEILNERKPMPAKIRELSFSKK
AVKTNKIRGFRIINSKKTGKINTSIPPDIAMCDKCIGEMFSKGNRRYLYPFTNCARCGPRFSIAYVLPYDRDKTSMDDFS
MCHDCNTEYTNLRDWRYHAQTNCCEDCGPKYWIVEIIKNEIVSHFSGEEAVKQAIGIIRAGGTVAIKGIGGIHLVCKPDP
DAIKKLRNLKDRPRKPFAIMAKDLETVKKFAYVGEVEERLLLSPARPIVLLRKRGDIFYDVSPGFHTIGVMLPYTGIHYL
LLEDFFVLVMTSGNISGEMLCADNFEALTKLSKLSDGLLLHDRKIVNRCDDSVIKVLAGKPVFVRKSRGFIPEVIKIPLR
NRKHPEILATGADLKSSFGFFREESFYGSQYLGDLSYYINQVQYQKMLKRFEKLFDLHARVVVTDLHPDYFSKTLGEEYA
ENRDCEIYSCQHHVAHIYSVMAEKALKKCIGVAFDGTGYGFDGKIWGGEFFSIDNSKCMRFAHLRYIPMPSGELAIEKPE
LMAYAYLIDSGVDLKDEKAHLLYENAPVKTSSAGRLFDAAAAVLDVCKENTFEGEAPMKLESVAKRHSESFSWVLDKSAL
PWQIDFRKMIRELYSEKDKNSISELASKFHRTLAEAISVVCGEIKKLTGEKNVCLSGGVFQNKLLLEVAVALLEKKGFNV
WFNEKVSPNDEGIAFGQIYWHVIGSDG
>Mature_747_residues
MQEIEVFEIMINGIVQGVGFRPFVATLAEDLGLHGYVENCESGVRIVLDCDETKLREFIEILNERKPMPAKIRELSFSKK
AVKTNKIRGFRIINSKKTGKINTSIPPDIAMCDKCIGEMFSKGNRRYLYPFTNCARCGPRFSIAYVLPYDRDKTSMDDFS
MCHDCNTEYTNLRDWRYHAQTNCCEDCGPKYWIVEIIKNEIVSHFSGEEAVKQAIGIIRAGGTVAIKGIGGIHLVCKPDP
DAIKKLRNLKDRPRKPFAIMAKDLETVKKFAYVGEVEERLLLSPARPIVLLRKRGDIFYDVSPGFHTIGVMLPYTGIHYL
LLEDFFVLVMTSGNISGEMLCADNFEALTKLSKLSDGLLLHDRKIVNRCDDSVIKVLAGKPVFVRKSRGFIPEVIKIPLR
NRKHPEILATGADLKSSFGFFREESFYGSQYLGDLSYYINQVQYQKMLKRFEKLFDLHARVVVTDLHPDYFSKTLGEEYA
ENRDCEIYSCQHHVAHIYSVMAEKALKKCIGVAFDGTGYGFDGKIWGGEFFSIDNSKCMRFAHLRYIPMPSGELAIEKPE
LMAYAYLIDSGVDLKDEKAHLLYENAPVKTSSAGRLFDAAAAVLDVCKENTFEGEAPMKLESVAKRHSESFSWVLDKSAL
PWQIDFRKMIRELYSEKDKNSISELASKFHRTLAEAISVVCGEIKKLTGEKNVCLSGGVFQNKLLLEVAVALLEKKGFNV
WFNEKVSPNDEGIAFGQIYWHVIGSDG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=761, Percent_Identity=33.7713534822602, Blast_Score=353, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 84527; Mature: 84527

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00136 SUBTILASE_ASP ; PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIEVFEIMINGIVQGVGFRPFVATLAEDLGLHGYVENCESGVRIVLDCDETKLREFIE
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCHHHHHHHHH
ILNERKPMPAKIRELSFSKKAVKTNKIRGFRIINSKKTGKINTSIPPDIAMCDKCIGEMF
HHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCHHHHHHHHHHHH
SKGNRRYLYPFTNCARCGPRFSIAYVLPYDRDKTSMDDFSMCHDCNTEYTNLRDWRYHAQ
HCCCCEEEECCCHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCEECCC
TNCCEDCGPKYWIVEIIKNEIVSHFSGEEAVKQAIGIIRAGGTVAIKGIGGIHLVCKPDP
CCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCEEEEEECCCEEEEECCCH
DAIKKLRNLKDRPRKPFAIMAKDLETVKKFAYVGEVEERLLLSPARPIVLLRKRGDIFYD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEE
VSPGFHTIGVMLPYTGIHYLLLEDFFVLVMTSGNISGEMLCADNFEALTKLSKLSDGLLL
CCCCCEEHHEECCHHHHHHHHHHHHHEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCEE
HDRKIVNRCDDSVIKVLAGKPVFVRKSRGFIPEVIKIPLRNRKHPEILATGADLKSSFGF
EHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCCCCCEEEECCCHHHHHCC
FREESFYGSQYLGDLSYYINQVQYQKMLKRFEKLFDLHARVVVTDLHPDYFSKTLGEEYA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHH
ENRDCEIYSCQHHVAHIYSVMAEKALKKCIGVAFDGTGYGFDGKIWGGEFFSIDNSKCMR
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCEEECCEEEECCCHHHHE
FAHLRYIPMPSGELAIEKPELMAYAYLIDSGVDLKDEKAHLLYENAPVKTSSAGRLFDAA
EEHEEEEECCCCCCEEECCHHHEEEEHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
AAVLDVCKENTFEGEAPMKLESVAKRHSESFSWVLDKSALPWQIDFRKMIRELYSEKDKN
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCEEEHHHHHHHHHHHCCCCH
SISELASKFHRTLAEAISVVCGEIKKLTGEKNVCLSGGVFQNKLLLEVAVALLEKKGFNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCE
WFNEKVSPNDEGIAFGQIYWHVIGSDG
EECCCCCCCCCCEEEEEEEEEEECCCC
>Mature Secondary Structure
MQEIEVFEIMINGIVQGVGFRPFVATLAEDLGLHGYVENCESGVRIVLDCDETKLREFIE
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCHHHHHHHHH
ILNERKPMPAKIRELSFSKKAVKTNKIRGFRIINSKKTGKINTSIPPDIAMCDKCIGEMF
HHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCHHHHHHHHHHHH
SKGNRRYLYPFTNCARCGPRFSIAYVLPYDRDKTSMDDFSMCHDCNTEYTNLRDWRYHAQ
HCCCCEEEECCCHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCEECCC
TNCCEDCGPKYWIVEIIKNEIVSHFSGEEAVKQAIGIIRAGGTVAIKGIGGIHLVCKPDP
CCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCEEEEEECCCEEEEECCCH
DAIKKLRNLKDRPRKPFAIMAKDLETVKKFAYVGEVEERLLLSPARPIVLLRKRGDIFYD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEE
VSPGFHTIGVMLPYTGIHYLLLEDFFVLVMTSGNISGEMLCADNFEALTKLSKLSDGLLL
CCCCCEEHHEECCHHHHHHHHHHHHHEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCEE
HDRKIVNRCDDSVIKVLAGKPVFVRKSRGFIPEVIKIPLRNRKHPEILATGADLKSSFGF
EHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCCCCCEEEECCCHHHHHCC
FREESFYGSQYLGDLSYYINQVQYQKMLKRFEKLFDLHARVVVTDLHPDYFSKTLGEEYA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHH
ENRDCEIYSCQHHVAHIYSVMAEKALKKCIGVAFDGTGYGFDGKIWGGEFFSIDNSKCMR
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCEEECCEEEECCCHHHHE
FAHLRYIPMPSGELAIEKPELMAYAYLIDSGVDLKDEKAHLLYENAPVKTSSAGRLFDAA
EEHEEEEECCCCCCEEECCHHHEEEEHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
AAVLDVCKENTFEGEAPMKLESVAKRHSESFSWVLDKSALPWQIDFRKMIRELYSEKDKN
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCEEEHHHHHHHHHHHCCCCH
SISELASKFHRTLAEAISVVCGEIKKLTGEKNVCLSGGVFQNKLLLEVAVALLEKKGFNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCE
WFNEKVSPNDEGIAFGQIYWHVIGSDG
EECCCCCCCCCCEEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]