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The map label for this gene is 239616890

Identifier: 239616890

GI number: 239616890

Start: 524065

End: 525126

Strand: Reverse

Name: 239616890

Synonym: Kole_0485

Alternate gene names: NA

Gene position: 525126-524065 (Counterclockwise)

Preceding gene: 239616895

Following gene: 239616870

Centisome position: 22.81

GC content: 43.13

Gene sequence:

>1062_bases
GTGAAGGTTATTAAGTTAAACATTGGGGGTTTGAAGCCAAAAATTCCTCTTATACAGGGTGGTATGGCTGTTGGTATATC
ACTTGACAATCTCGCTTCAGCGGTTGCCAATGAAGGCGGGATAGGGGTTATAGGCACAGCCGGCATAGGAATGATGGTGG
GAGGTAATAGAAGGAATTTTGAGCAGGCGAGCATAGAAGGCTTAAAAAGGATCATTCGAAATGCTCGCGAAAAAACCAAC
GGCATTCTTGGTGTGAATATCATGGTTGCATTGACCAATTATAAGGATATGGTGACTACGGCGATCAAGGAAAAGATAGA
TGTCATAATTTCTGGAGCCGGATTGCCGCTGGATCTGCCTTCTTTTTTAAGTGAGGGTTCAAAAACCAGGTTGATTCCAA
TAGTATCTTCGTTGAGATCTGCTCAACTTATTTTCAGGAGATGGTTGAAAAGGCATAACTATATACCGGATGCTTTTGTG
GTTGAAGGACCAAAAGCCGGAGGACATTTAGGATACCGTCCAGAACAGCTCGATGATCCTGAGTCTTCACTTGAGAGGAC
TATTCCTCAGATACGTGATTTTACCGAAGAGATTAAAAGGGAGTATGGTAAGGAGGTTCCTGTTATTGCTGCCGGGGGGA
TATACAGCTCTGATGACGTGAAAAGAGTGTTCTCGTTGGGAGCGTCTGGAATACAGGTAGGAACCCCGTTTATTGCAACT
GAGGAATGCGATGCGGATATCAGGTTTAAGGAAACGATCGTGAACGCTAAAAAAGAGGACATAGTGATTATCAAAAGCCC
GGTTGGTTTACCCGGTAGGGCTGTGCGGAATAAATTCATCGAAGATGTTGAAGCGGGAAAGAAAAAGCCATTCAGATGTG
GGTTTCATTGTATAAAGACGTGTAATGTCAAAGATGCTCCATATTGTATAGCCGAGGCCCTTTTGAATGCTTCAAAAGGG
AACCTTGAAGATGGGTTTGCTTTTATCGGAGCTATGGGATATAAAATCAACAAAATCACGACCGTTAAGGAAGTAATCAG
AAAGCTTTTTAAGGGAGTGTGA

Upstream 100 bases:

>100_bases
CTTTTTTTTGGGATATGGTTTTATGTTAGAATTCTCCTAAAGTAAAACAAGTAAAATGTAGAAATCGGAAATCCTTATTT
TGTTTTGATTTGAAAGGAGC

Downstream 100 bases:

>100_bases
TAAACCACTAGATCAGAGAATCCGAGAGACCGAGAAAGCCGCTTCACGGCGGATACGAATCCCTTCGGGATTGTCACGAA
CTCCTTCGGAGTTGACCCGA

Product: 2-nitropropane dioxygenase NPD

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKVIKLNIGGLKPKIPLIQGGMAVGISLDNLASAVANEGGIGVIGTAGIGMMVGGNRRNFEQASIEGLKRIIRNAREKTN
GILGVNIMVALTNYKDMVTTAIKEKIDVIISGAGLPLDLPSFLSEGSKTRLIPIVSSLRSAQLIFRRWLKRHNYIPDAFV
VEGPKAGGHLGYRPEQLDDPESSLERTIPQIRDFTEEIKREYGKEVPVIAAGGIYSSDDVKRVFSLGASGIQVGTPFIAT
EECDADIRFKETIVNAKKEDIVIIKSPVGLPGRAVRNKFIEDVEAGKKKPFRCGFHCIKTCNVKDAPYCIAEALLNASKG
NLEDGFAFIGAMGYKINKITTVKEVIRKLFKGV

Sequences:

>Translated_353_residues
MKVIKLNIGGLKPKIPLIQGGMAVGISLDNLASAVANEGGIGVIGTAGIGMMVGGNRRNFEQASIEGLKRIIRNAREKTN
GILGVNIMVALTNYKDMVTTAIKEKIDVIISGAGLPLDLPSFLSEGSKTRLIPIVSSLRSAQLIFRRWLKRHNYIPDAFV
VEGPKAGGHLGYRPEQLDDPESSLERTIPQIRDFTEEIKREYGKEVPVIAAGGIYSSDDVKRVFSLGASGIQVGTPFIAT
EECDADIRFKETIVNAKKEDIVIIKSPVGLPGRAVRNKFIEDVEAGKKKPFRCGFHCIKTCNVKDAPYCIAEALLNASKG
NLEDGFAFIGAMGYKINKITTVKEVIRKLFKGV
>Mature_353_residues
MKVIKLNIGGLKPKIPLIQGGMAVGISLDNLASAVANEGGIGVIGTAGIGMMVGGNRRNFEQASIEGLKRIIRNAREKTN
GILGVNIMVALTNYKDMVTTAIKEKIDVIISGAGLPLDLPSFLSEGSKTRLIPIVSSLRSAQLIFRRWLKRHNYIPDAFV
VEGPKAGGHLGYRPEQLDDPESSLERTIPQIRDFTEEIKREYGKEVPVIAAGGIYSSDDVKRVFSLGASGIQVGTPFIAT
EECDADIRFKETIVNAKKEDIVIIKSPVGLPGRAVRNKFIEDVEAGKKKPFRCGFHCIKTCNVKDAPYCIAEALLNASKG
NLEDGFAFIGAMGYKINKITTVKEVIRKLFKGV

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 38312; Mature: 38312

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIKLNIGGLKPKIPLIQGGMAVGISLDNLASAVANEGGIGVIGTAGIGMMVGGNRRNF
CEEEEEECCCCCCCCCEEECCEEEEEEHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCH
EQASIEGLKRIIRNAREKTNGILGVNIMVALTNYKDMVTTAIKEKIDVIISGAGLPLDLP
HHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHEEEECCCCCCCCH
SFLSEGSKTRLIPIVSSLRSAQLIFRRWLKRHNYIPDAFVVEGPKAGGHLGYRPEQLDDP
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
ESSLERTIPQIRDFTEEIKREYGKEVPVIAAGGIYSSDDVKRVFSLGASGIQVGTPFIAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEECCCEEEE
EECDADIRFKETIVNAKKEDIVIIKSPVGLPGRAVRNKFIEDVEAGKKKPFRCGFHCIKT
CCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHCHHHHEE
CNVKDAPYCIAEALLNASKGNLEDGFAFIGAMGYKINKITTVKEVIRKLFKGV
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVIKLNIGGLKPKIPLIQGGMAVGISLDNLASAVANEGGIGVIGTAGIGMMVGGNRRNF
CEEEEEECCCCCCCCCEEECCEEEEEEHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCH
EQASIEGLKRIIRNAREKTNGILGVNIMVALTNYKDMVTTAIKEKIDVIISGAGLPLDLP
HHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHEEEECCCCCCCCH
SFLSEGSKTRLIPIVSSLRSAQLIFRRWLKRHNYIPDAFVVEGPKAGGHLGYRPEQLDDP
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
ESSLERTIPQIRDFTEEIKREYGKEVPVIAAGGIYSSDDVKRVFSLGASGIQVGTPFIAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEECCCEEEE
EECDADIRFKETIVNAKKEDIVIIKSPVGLPGRAVRNKFIEDVEAGKKKPFRCGFHCIKT
CCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHCHHHHEE
CNVKDAPYCIAEALLNASKGNLEDGFAFIGAMGYKINKITTVKEVIRKLFKGV
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA