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The map label for this gene is ykfA [H]

Identifier: 239616712

GI number: 239616712

Start: 331344

End: 332351

Strand: Direct

Name: ykfA [H]

Synonym: Kole_0305

Alternate gene names: 239616712

Gene position: 331344-332351 (Clockwise)

Preceding gene: 239616711

Following gene: 239616734

Centisome position: 14.39

GC content: 39.58

Gene sequence:

>1008_bases
TTGATTTGTGCGGACGGTGAAGTAGTGATGAAAAGAATTCTTTTATTGCTTTTGATTTTTTCCATAATCCTATCCTTTAT
GCAACTATCTGCAAACGTCAGCAAAAGAATCTCTGTTTGGAACTTCATTCATAAAAACAAAGTGAATACAGTTTTTGTAA
CTTCACCGGCTCGTTTTCCTGACCAGACCCTACTCGATGCAGGAATAAGAACACTCCAAAGTGCAGGGTTAACAGTCATA
GTAGGCGACAGTTGCCGAAGATATCTATCTGACGAAGAAAAAGCCAGAGAAATCAACGAAGCCTTTGCAGATCCGAATAT
TGACATGATAATTACATCTCGCGGTGGCCATGGATCGTTCAGAGTTCTGGATTACCTGGACTGGAAAACCATCTCAAAAA
ATCCTAAACCTATCGTCGGATACAGTGATATCACAGCTTTGCTTTTAGGAATCTATTTCAAGACAGGTATCATCACCTAC
CACGGCCCAATGGTAACCGTTGAACTAGATAATGACGGAAAATCACTTGAGAAAATGCTGAAACTTTTGAACGGTGATAA
ATTGATTACCTTTGATTATAATTCAAAGCCGATAGTACCTGGTAAAATGGTTGGAAGGCTGATCGTAGGCAACCTGTCCC
TTTTCAAAACTCTTCAAGGTACAAAATATTTTGGTTCTCTGAAAGATTCCATTTTGGTTCTTGAAGATGTAGGAGAAAGC
CGGGAGTCAATAGAACGCATGGTATGGAATATAGCACATCTGAAAGACTATCAAACCTTAAGGGGAATAGTTTTTGCGGG
ATTCACCCAGATAAATAACGATGACTTTGACGGTGTTTTCAAAATCCTCGAAAGATTTTTCGAAGATGCCCCATTTCCTG
TTTGGGTAGGACTTCCAGTATTCCACGGAAATTTTACAAAGCTAACTTTGCCCGTTGGTGCATGGGTTGAGATGGATCTC
GAGAATCACTATATAAAGATTTTGAATGGTCCTGAACCAGAGAGATAA

Upstream 100 bases:

>100_bases
ATTCGCCAGTGTATAGAATTGATGCAAAATTCGTGGATATTGAAAGATATCCAGATCATCTGGACAAAATTGTTTCCGAC
GTCAAAAGAATTTTGAAGAT

Downstream 100 bases:

>100_bases
AAAAAGAGGGAACCCAACAGTGAGTTCCCTCTTTTTTTACTTAGTTCTTTATTCTTCATCAAAGTAGGTTCTTGATGCAT
GGCTTACAGCTATTGCACCG

Product: peptidase U61 LD-carboxypeptidase A

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MICADGEVVMKRILLLLLIFSIILSFMQLSANVSKRISVWNFIHKNKVNTVFVTSPARFPDQTLLDAGIRTLQSAGLTVI
VGDSCRRYLSDEEKAREINEAFADPNIDMIITSRGGHGSFRVLDYLDWKTISKNPKPIVGYSDITALLLGIYFKTGIITY
HGPMVTVELDNDGKSLEKMLKLLNGDKLITFDYNSKPIVPGKMVGRLIVGNLSLFKTLQGTKYFGSLKDSILVLEDVGES
RESIERMVWNIAHLKDYQTLRGIVFAGFTQINNDDFDGVFKILERFFEDAPFPVWVGLPVFHGNFTKLTLPVGAWVEMDL
ENHYIKILNGPEPER

Sequences:

>Translated_335_residues
MICADGEVVMKRILLLLLIFSIILSFMQLSANVSKRISVWNFIHKNKVNTVFVTSPARFPDQTLLDAGIRTLQSAGLTVI
VGDSCRRYLSDEEKAREINEAFADPNIDMIITSRGGHGSFRVLDYLDWKTISKNPKPIVGYSDITALLLGIYFKTGIITY
HGPMVTVELDNDGKSLEKMLKLLNGDKLITFDYNSKPIVPGKMVGRLIVGNLSLFKTLQGTKYFGSLKDSILVLEDVGES
RESIERMVWNIAHLKDYQTLRGIVFAGFTQINNDDFDGVFKILERFFEDAPFPVWVGLPVFHGNFTKLTLPVGAWVEMDL
ENHYIKILNGPEPER
>Mature_335_residues
MICADGEVVMKRILLLLLIFSIILSFMQLSANVSKRISVWNFIHKNKVNTVFVTSPARFPDQTLLDAGIRTLQSAGLTVI
VGDSCRRYLSDEEKAREINEAFADPNIDMIITSRGGHGSFRVLDYLDWKTISKNPKPIVGYSDITALLLGIYFKTGIITY
HGPMVTVELDNDGKSLEKMLKLLNGDKLITFDYNSKPIVPGKMVGRLIVGNLSLFKTLQGTKYFGSLKDSILVLEDVGES
RESIERMVWNIAHLKDYQTLRGIVFAGFTQINNDDFDGVFKILERFFEDAPFPVWVGLPVFHGNFTKLTLPVGAWVEMDL
ENHYIKILNGPEPER

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=265, Percent_Identity=27.9245283018868, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 37770; Mature: 37770

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICADGEVVMKRILLLLLIFSIILSFMQLSANVSKRISVWNFIHKNKVNTVFVTSPARFP
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
DQTLLDAGIRTLQSAGLTVIVGDSCRRYLSDEEKAREINEAFADPNIDMIITSRGGHGSF
CHHHHHHHHHHHHHCCCEEEECHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCE
RVLDYLDWKTISKNPKPIVGYSDITALLLGIYFKTGIITYHGPMVTVELDNDGKSLEKML
EEEEEECCHHCCCCCCCEECHHHHHHHHHHHHHHHCEEEEECCEEEEEECCCCHHHHHHH
KLLNGDKLITFDYNSKPIVPGKMVGRLIVGNLSLFKTLQGTKYFGSLKDSILVLEDVGES
HHHCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHCCCHHHCCCCCCEEEEECCCCH
RESIERMVWNIAHLKDYQTLRGIVFAGFTQINNDDFDGVFKILERFFEDAPFPVWVGLPV
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEECEE
FHGNFTKLTLPVGAWVEMDLENHYIKILNGPEPER
EECCCEEEEEECCCEEEEECCCCEEEEECCCCCCC
>Mature Secondary Structure
MICADGEVVMKRILLLLLIFSIILSFMQLSANVSKRISVWNFIHKNKVNTVFVTSPARFP
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
DQTLLDAGIRTLQSAGLTVIVGDSCRRYLSDEEKAREINEAFADPNIDMIITSRGGHGSF
CHHHHHHHHHHHHHCCCEEEECHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCE
RVLDYLDWKTISKNPKPIVGYSDITALLLGIYFKTGIITYHGPMVTVELDNDGKSLEKML
EEEEEECCHHCCCCCCCEECHHHHHHHHHHHHHHHCEEEEECCEEEEEECCCCHHHHHHH
KLLNGDKLITFDYNSKPIVPGKMVGRLIVGNLSLFKTLQGTKYFGSLKDSILVLEDVGES
HHHCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHCCCHHHCCCCCCEEEEECCCCH
RESIERMVWNIAHLKDYQTLRGIVFAGFTQINNDDFDGVFKILERFFEDAPFPVWVGLPV
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEECEE
FHGNFTKLTLPVGAWVEMDLENHYIKILNGPEPER
EECCCEEEEEECCCEEEEECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]