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The map label for this gene is miaA

Identifier: 239616687

GI number: 239616687

Start: 307158

End: 308084

Strand: Direct

Name: miaA

Synonym: Kole_0280

Alternate gene names: 239616687

Gene position: 307158-308084 (Clockwise)

Preceding gene: 239616686

Following gene: 239616688

Centisome position: 13.34

GC content: 39.48

Gene sequence:

>927_bases
ATGAAGATACCAATGATATTAGGGGCAACAGCTGTCGGTAAAACACAATTTCTGGTAAAACTCGCTTCATTAATTCCAAT
AGAGGTAGTATCCGTTGATTCAAGGCAGATATATAGATACATGGATATTGGGACAGCAAAACCTACACGGGAAGAACTTT
CAAAATTAAAACATCATGTTATAGACGTTGTAGATCCGGACGAAGATTTCAATGTTTTTGAATACCGTAAAATTGCTCTG
AAAGCCATGGACGAGATTGTCAAGAAAGGGAGAATCCCCGTTTTTGCTGGGGGAAGTGGTCTTTATGCTGAAACACTGAT
GAAAGGGATAGTAGAAAATGTTCCACGAAATGATAAAGTCAGGGAAGCATTGAAAACGCTTGAAGTGAATGCTCCAGGAT
CTTTGAGAAAACTCCTCGAAAAAGTGGATCATGAAGCCTACGAAAAGATTCATCCAAACGATTTGAAAAGAACTATCCGG
TATCTTGAAGTGTTTTTTACCACCGGGAAAACATTAACGTCACTTCAGAAAATACATAAATGCTCAAATCAATTCACTGT
TATAATATTAGAAAGAGATAGAAGAGAACTTGCCGAAAGAATTGAGAACCGTGTCGATAAAATGGTCGAATCGGGGTTGA
TCGAGGAAGTAGTAAGGTTAAAAGAAATGGGTTACACCCGTGAGTTGAATTCCCAGCAAACGATCGGTTATGCGGAGATC
TGGAGCTACCTTGAAGGGGAGGTCACTTTAGAAAGAGCCACGGAGCTCATAAAACGAAATACAAGACGTTTTGCGAGACG
CCAGATTATCTGGTTCAGACGGTACAAAGATGCAAAACGAATCAGTCTTTCTGAAACCAATGAGAATGATATCTTGAGTC
TTTTGAAGGAAGACATTCTTAATGTTTGGGGGGGTAAATATGGCTGA

Upstream 100 bases:

>100_bases
GCAATACCGATAGTCTCTTTGATTCATTCAAAACCATAAAGTTCGACAGAATATCGGGAGGTAAAAGCAGCGGCGTTTTG
TATGGAGTGATAGAGAAGAA

Downstream 100 bases:

>100_bases
AAAATTCAATCTTCAGGATCGTTTCCTGAATCTCTTGAGGATCAACAAGATCGAGGTTAAGATTTTCCTTGAAGGTGGGT
TTCAAACGAAGGGAATCGTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKIPMILGATAVGKTQFLVKLASLIPIEVVSVDSRQIYRYMDIGTAKPTREELSKLKHHVIDVVDPDEDFNVFEYRKIAL
KAMDEIVKKGRIPVFAGGSGLYAETLMKGIVENVPRNDKVREALKTLEVNAPGSLRKLLEKVDHEAYEKIHPNDLKRTIR
YLEVFFTTGKTLTSLQKIHKCSNQFTVIILERDRRELAERIENRVDKMVESGLIEEVVRLKEMGYTRELNSQQTIGYAEI
WSYLEGEVTLERATELIKRNTRRFARRQIIWFRRYKDAKRISLSETNENDILSLLKEDILNVWGGKYG

Sequences:

>Translated_308_residues
MKIPMILGATAVGKTQFLVKLASLIPIEVVSVDSRQIYRYMDIGTAKPTREELSKLKHHVIDVVDPDEDFNVFEYRKIAL
KAMDEIVKKGRIPVFAGGSGLYAETLMKGIVENVPRNDKVREALKTLEVNAPGSLRKLLEKVDHEAYEKIHPNDLKRTIR
YLEVFFTTGKTLTSLQKIHKCSNQFTVIILERDRRELAERIENRVDKMVESGLIEEVVRLKEMGYTRELNSQQTIGYAEI
WSYLEGEVTLERATELIKRNTRRFARRQIIWFRRYKDAKRISLSETNENDILSLLKEDILNVWGGKYG
>Mature_308_residues
MKIPMILGATAVGKTQFLVKLASLIPIEVVSVDSRQIYRYMDIGTAKPTREELSKLKHHVIDVVDPDEDFNVFEYRKIAL
KAMDEIVKKGRIPVFAGGSGLYAETLMKGIVENVPRNDKVREALKTLEVNAPGSLRKLLEKVDHEAYEKIHPNDLKRTIR
YLEVFFTTGKTLTSLQKIHKCSNQFTVIILERDRRELAERIENRVDKMVESGLIEEVVRLKEMGYTRELNSQQTIGYAEI
WSYLEGEVTLERATELIKRNTRRFARRQIIWFRRYKDAKRISLSETNENDILSLLKEDILNVWGGKYG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=32.3624595469256, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1790613, Length=313, Percent_Identity=37.0607028753994, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=27.3333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=30.9027777777778, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24649830, Length=353, Percent_Identity=30.028328611898, Blast_Score=146, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_KOSOT (C5CD61)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002940009.1
- GeneID:   7969032
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_0280
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35673; Mature: 35673

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPMILGATAVGKTQFLVKLASLIPIEVVSVDSRQIYRYMDIGTAKPTREELSKLKHHV
CCCCEEECCHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHH
IDVVDPDEDFNVFEYRKIALKAMDEIVKKGRIPVFAGGSGLYAETLMKGIVENVPRNDKV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCHHH
REALKTLEVNAPGSLRKLLEKVDHEAYEKIHPNDLKRTIRYLEVFFTTGKTLTSLQKIHK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
CSNQFTVIILERDRRELAERIENRVDKMVESGLIEEVVRLKEMGYTRELNSQQTIGYAEI
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHH
WSYLEGEVTLERATELIKRNTRRFARRQIIWFRRYKDAKRISLSETNENDILSLLKEDIL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHH
NVWGGKYG
HHCCCCCC
>Mature Secondary Structure
MKIPMILGATAVGKTQFLVKLASLIPIEVVSVDSRQIYRYMDIGTAKPTREELSKLKHHV
CCCCEEECCHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHH
IDVVDPDEDFNVFEYRKIALKAMDEIVKKGRIPVFAGGSGLYAETLMKGIVENVPRNDKV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCHHH
REALKTLEVNAPGSLRKLLEKVDHEAYEKIHPNDLKRTIRYLEVFFTTGKTLTSLQKIHK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
CSNQFTVIILERDRRELAERIENRVDKMVESGLIEEVVRLKEMGYTRELNSQQTIGYAEI
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHH
WSYLEGEVTLERATELIKRNTRRFARRQIIWFRRYKDAKRISLSETNENDILSLLKEDIL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHH
NVWGGKYG
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA