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The map label for this gene is spo0F [H]

Identifier: 239616421

GI number: 239616421

Start: 10636

End: 10995

Strand: Direct

Name: spo0F [H]

Synonym: Kole_0010

Alternate gene names: 239616421

Gene position: 10636-10995 (Clockwise)

Preceding gene: 239616420

Following gene: 239616422

Centisome position: 0.46

GC content: 42.5

Gene sequence:

>360_bases
ATGGAGACGAAAAAAATAATGATCGTAGATGATGAAGAGAACATGCGTTTTTTGTTGACTGAAGAGCTTGGTGAAGAAGG
ATTTCACGTCATAACCGCTGCTTCCGCAGAAGAAGCCCTGGACCTTTTCGAGAAACATCCGGATATTTCCCTCGTTACCA
TAGACATCGAGATGCCAGGAATCAGCGGCCTTGAATTAGCAGGCATGCTTAGAAATAAAAAACCTGACCTTAAAATTGTG
TTGCTCACCGCCTACACACATTACAAACAGGATATGGCCTCATGGGCTGCAGACGCCTATGTGGTAAAATCCACGGATTT
GCGTGAATTCAAAGAAACGATAAAAAAATTGCTTGATTGA

Upstream 100 bases:

>100_bases
TGTAAACGAATAGTCGAAGAACATGGTGGAAACATAACAGCTCAGAGTGATTCTAACGGTACTATCTTCGTGGTATCATT
GCCCTCAAGGAGGTATGCGA

Downstream 100 bases:

>100_bases
TACGGAGGTGCATCATGGATTATAAGGACACTCTGAATCTTCCCAAAACATCTTTTTCTATGCGTGCCAATCTTGTCAGG
AAGGAACCTGAACAACTGAA

Product: response regulator receiver protein

Products: NA

Alternate protein names: Stage 0 sporulation protein F [H]

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
METKKIMIVDDEENMRFLLTEELGEEGFHVITAASAEEALDLFEKHPDISLVTIDIEMPGISGLELAGMLRNKKPDLKIV
LLTAYTHYKQDMASWAADAYVVKSTDLREFKETIKKLLD

Sequences:

>Translated_119_residues
METKKIMIVDDEENMRFLLTEELGEEGFHVITAASAEEALDLFEKHPDISLVTIDIEMPGISGLELAGMLRNKKPDLKIV
LLTAYTHYKQDMASWAADAYVVKSTDLREFKETIKKLLD
>Mature_119_residues
METKKIMIVDDEENMRFLLTEELGEEGFHVITAASAEEALDLFEKHPDISLVTIDIEMPGISGLELAGMLRNKKPDLKIV
LLTAYTHYKQDMASWAADAYVVKSTDLREFKETIKKLLD

Specific function: Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation [H]

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=32.2033898305085, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13517; Mature: 13517

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKKIMIVDDEENMRFLLTEELGEEGFHVITAASAEEALDLFEKHPDISLVTIDIEMPG
CCCCEEEEEECCCCCEEEEEHHCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEECCC
ISGLELAGMLRNKKPDLKIVLLTAYTHYKQDMASWAADAYVVKSTDLREFKETIKKLLD
CCCHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
METKKIMIVDDEENMRFLLTEELGEEGFHVITAASAEEALDLFEKHPDISLVTIDIEMPG
CCCCEEEEEECCCCCEEEEEHHCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEECCC
ISGLELAGMLRNKKPDLKIVLLTAYTHYKQDMASWAADAYVVKSTDLREFKETIKKLLD
CCCHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3003689; 2997779; 2457578; 9353933; 9384377; 8528078; 9254596; 8805550; 9335530; 10997904 [H]