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The map label for this gene is recG [H]

Identifier: 239616417

GI number: 239616417

Start: 4561

End: 6924

Strand: Direct

Name: recG [H]

Synonym: Kole_0006

Alternate gene names: 239616417

Gene position: 4561-6924 (Clockwise)

Preceding gene: 239616416

Following gene: 239616418

Centisome position: 0.2

GC content: 44.59

Gene sequence:

>2364_bases
ATGCTGTTAGAAGATTATCTGGATCGTTGCGAGAAACTTTTAGAAAAGTTTTTAAGCGGAAAGGTTGATACAATTGAGTT
AATCCAGGAAATCAAGCGGGGAGCAGAACTCCTTGAACCAGAGGAGTTATCTGCAAATGAGGGATTAAAAGACTACATCG
GAAAGTTTGTCTCTTATTATCTTCCACTGGCATCACTTCCGAATGAACGGCAACACCTTCGTGTAAAAAATGGTTTCACC
ATGATCAACAAACTCAGGGGTAAATTTCTTCTAACATCCTTTGATGAAAGCACTTCCGCTATACCCGTGAAGGTTTCTTC
GCTGAACTTTGACATAAAATATGCAAAAGGTGTTGGAGAAAGGCGCGCGAAGATTCTGAAAAAGCTTGGTATAGAAACAG
TTAAGGACCTGCTCTGGTGGTTACCGAGAGATTATGAAGACCGTCGAAGAATCGTTCCACTGTCTTCCATTGTGGCTGAC
CGCAAGGTAACTATCAGAGCGAAACTTCAGAACTTTAGCGTTAAAAAGGTAAAGGAATACGTGATTATTTCTGCTGTTGT
TTCCGATGGATTTGGACAGATCATTCTGAAGTGGTTCAACCAGGAATACATAACGGACAGACTTATAAAAGAAAGGGAGT
ACCTCATTACAGGCATCCCCAAAAAAACACCCTTTGGCCCCTACGAAATGAATTCTCCTGAGATAGAGGAGATAACCGGG
CGTGTTCCCCGGGAGATTCTCCCGCTTTACAGCCTTTCCGCCGGGATCTCCCAAAAGGTAATGCGGAAAATCGTGCGAAA
AAATATCACCAACGTACGCTTGCTGAAAGAGTTTATTCCTGCCGAAGTTATCAAAGAGAGAAATCTGCTGCCAAGACATC
ATGCCATGTACACCGTGCATTTTCCAAAGAGCCTATATGAGTTGAAAGAATCCAGAAGGCGCCTGGCATATGAAGAGCTG
TTTCTTTTTGAGGTTGCTGTTCTTTACAACCGGGAAAAGTTGAAAACCACGAAAGGTGGAATCAGCAAGAGCATATCCGG
AAAACTTGCCGAAAGATTCATCGACAGTCTCAACTTTGTCCTTACAGGCGACCAGATGAGGGCCTTTGAAGAGATAAGGG
AGGATATGAAAGCTCCCACGCCTATGAATCGATTACTTCAGGGCGACGTTGGTTCAGGAAAAACGGTTGTTGCTGAGCTG
GCTATTATTGATAATTTTGAGGCAGGCTACCAGAGTGCGATGATGGTTCCAACTACCGTCCTTGCAACCCAGCAGCATCA
AAAATTGGTGAAGGATTTAGAACCGCTTGGTATAAAAGTCGAACTGCTTGTCGGTTCTCAAAAAAAATCGCAGCAGGAAG
AAATAAAGAAACGAATAGCAATAGGAGAGGTTGATGTTGTTGTTGGAACACACGCCCTGATCCAGGAGAATGTGAAGTTT
AAAGACCTGGGGCTCGTTATAATTGATGAACAACACAGGTTTGGAGTAAAGCAACGTGAAGCTCTCATGAACAAAGGAGC
ACTTGTCGACACACTCGTTATGACCGCAACTCCAATCCCGCGAACCCTGGCATTGACCGCATATGGCGATCTCGATATCT
CCACCATTACTGAAATGCCCCCAGGGAGAGCCCCCATCAGGACGATGCTGATAAGCGAAAAACGCTTACCGGAACTCTAT
GCCTTCATTCGCGATGAAGTAAATCACGGTCATCAGGCCTTCTTTATCTATCCTTTAATAGAAGAATCCGAGCAAATGGA
TCTGAAAGCAGCGACAGATGAGGCCGAGAGATTGCAAAAGGAAGTGTTCCCGGATATCGGGGTTGAACTGCTTCATGGCA
GGATGAGCGATGAAGAGAAAAACAGGATCATGCACCGCTTCAAAAACAAAGAGGCAATGATTCTCGTGTCGACCTCTGTT
GTTGAGGTCGGGATAGACATCCCTACAGCCACCGTTATGGTAATAGAACATCCCGAAAGATTTGGACTGGCGCAGCTCCA
TCAGTTGAGAGGAAGGGTTGGTCGCAGTTCTTTGAAATCTTACTGTATGCTCGTTCTTAATAGCAACATCTCCGGAGAAG
CTCTCGACAGGTTGAGAAAATTTGCGGGTACACAAAACGGATTTAAGCTCGCTGAGATAGATCTGAGCCTCAGAGGCCCC
GGGGAGTTTATGGGGACGAGACAACATGGCCTTCCGGACTTTCTGGTGGCTGATATTGTTAAAGACAGTGAACTCCTAAT
TATGGCGCGTAACGATGCCATGGAATTGCTGAAAAGGGATCCGAATCTTGAAAAACATAATAGAATAATAGAAGAAATAA
AGGAACGATTTGGTGAAAATATATCTCTCATCGAGGTAGGCTGA

Upstream 100 bases:

>100_bases
TCACCCGTAAACCGCATTATTAGTTGAACCACAAATCCTCTTCGAAAGTCAAATCACAACACGCCATGACCCTTAAAAAA
ATTTGCAGGGAGAATACTCA

Downstream 100 bases:

>100_bases
TGCTGACAATAACCGGAGGGAACTGGAAAGGAAGAAAGGTTGAAGTAACGCCCAGGCTGATAACAAGATATACTCCCCAA
TTTGCGAGAAAAGCGCTCTT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MLLEDYLDRCEKLLEKFLSGKVDTIELIQEIKRGAELLEPEELSANEGLKDYIGKFVSYYLPLASLPNERQHLRVKNGFT
MINKLRGKFLLTSFDESTSAIPVKVSSLNFDIKYAKGVGERRAKILKKLGIETVKDLLWWLPRDYEDRRRIVPLSSIVAD
RKVTIRAKLQNFSVKKVKEYVIISAVVSDGFGQIILKWFNQEYITDRLIKEREYLITGIPKKTPFGPYEMNSPEIEEITG
RVPREILPLYSLSAGISQKVMRKIVRKNITNVRLLKEFIPAEVIKERNLLPRHHAMYTVHFPKSLYELKESRRRLAYEEL
FLFEVAVLYNREKLKTTKGGISKSISGKLAERFIDSLNFVLTGDQMRAFEEIREDMKAPTPMNRLLQGDVGSGKTVVAEL
AIIDNFEAGYQSAMMVPTTVLATQQHQKLVKDLEPLGIKVELLVGSQKKSQQEEIKKRIAIGEVDVVVGTHALIQENVKF
KDLGLVIIDEQHRFGVKQREALMNKGALVDTLVMTATPIPRTLALTAYGDLDISTITEMPPGRAPIRTMLISEKRLPELY
AFIRDEVNHGHQAFFIYPLIEESEQMDLKAATDEAERLQKEVFPDIGVELLHGRMSDEEKNRIMHRFKNKEAMILVSTSV
VEVGIDIPTATVMVIEHPERFGLAQLHQLRGRVGRSSLKSYCMLVLNSNISGEALDRLRKFAGTQNGFKLAEIDLSLRGP
GEFMGTRQHGLPDFLVADIVKDSELLIMARNDAMELLKRDPNLEKHNRIIEEIKERFGENISLIEVG

Sequences:

>Translated_787_residues
MLLEDYLDRCEKLLEKFLSGKVDTIELIQEIKRGAELLEPEELSANEGLKDYIGKFVSYYLPLASLPNERQHLRVKNGFT
MINKLRGKFLLTSFDESTSAIPVKVSSLNFDIKYAKGVGERRAKILKKLGIETVKDLLWWLPRDYEDRRRIVPLSSIVAD
RKVTIRAKLQNFSVKKVKEYVIISAVVSDGFGQIILKWFNQEYITDRLIKEREYLITGIPKKTPFGPYEMNSPEIEEITG
RVPREILPLYSLSAGISQKVMRKIVRKNITNVRLLKEFIPAEVIKERNLLPRHHAMYTVHFPKSLYELKESRRRLAYEEL
FLFEVAVLYNREKLKTTKGGISKSISGKLAERFIDSLNFVLTGDQMRAFEEIREDMKAPTPMNRLLQGDVGSGKTVVAEL
AIIDNFEAGYQSAMMVPTTVLATQQHQKLVKDLEPLGIKVELLVGSQKKSQQEEIKKRIAIGEVDVVVGTHALIQENVKF
KDLGLVIIDEQHRFGVKQREALMNKGALVDTLVMTATPIPRTLALTAYGDLDISTITEMPPGRAPIRTMLISEKRLPELY
AFIRDEVNHGHQAFFIYPLIEESEQMDLKAATDEAERLQKEVFPDIGVELLHGRMSDEEKNRIMHRFKNKEAMILVSTSV
VEVGIDIPTATVMVIEHPERFGLAQLHQLRGRVGRSSLKSYCMLVLNSNISGEALDRLRKFAGTQNGFKLAEIDLSLRGP
GEFMGTRQHGLPDFLVADIVKDSELLIMARNDAMELLKRDPNLEKHNRIIEEIKERFGENISLIEVG
>Mature_787_residues
MLLEDYLDRCEKLLEKFLSGKVDTIELIQEIKRGAELLEPEELSANEGLKDYIGKFVSYYLPLASLPNERQHLRVKNGFT
MINKLRGKFLLTSFDESTSAIPVKVSSLNFDIKYAKGVGERRAKILKKLGIETVKDLLWWLPRDYEDRRRIVPLSSIVAD
RKVTIRAKLQNFSVKKVKEYVIISAVVSDGFGQIILKWFNQEYITDRLIKEREYLITGIPKKTPFGPYEMNSPEIEEITG
RVPREILPLYSLSAGISQKVMRKIVRKNITNVRLLKEFIPAEVIKERNLLPRHHAMYTVHFPKSLYELKESRRRLAYEEL
FLFEVAVLYNREKLKTTKGGISKSISGKLAERFIDSLNFVLTGDQMRAFEEIREDMKAPTPMNRLLQGDVGSGKTVVAEL
AIIDNFEAGYQSAMMVPTTVLATQQHQKLVKDLEPLGIKVELLVGSQKKSQQEEIKKRIAIGEVDVVVGTHALIQENVKF
KDLGLVIIDEQHRFGVKQREALMNKGALVDTLVMTATPIPRTLALTAYGDLDISTITEMPPGRAPIRTMLISEKRLPELY
AFIRDEVNHGHQAFFIYPLIEESEQMDLKAATDEAERLQKEVFPDIGVELLHGRMSDEEKNRIMHRFKNKEAMILVSTSV
VEVGIDIPTATVMVIEHPERFGLAQLHQLRGRVGRSSLKSYCMLVLNSNISGEALDRLRKFAGTQNGFKLAEIDLSLRGP
GEFMGTRQHGLPDFLVADIVKDSELLIMARNDAMELLKRDPNLEKHNRIIEEIKERFGENISLIEVG

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=672, Percent_Identity=38.6904761904762, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1787357, Length=383, Percent_Identity=38.9033942558747, Blast_Score=262, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 89695; Mature: 89695

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEDYLDRCEKLLEKFLSGKVDTIELIQEIKRGAELLEPEELSANEGLKDYIGKFVSYY
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LPLASLPNERQHLRVKNGFTMINKLRGKFLLTSFDESTSAIPVKVSSLNFDIKYAKGVGE
HHHHCCCCCHHHEEECCCHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEEEEEECCCCCH
RRAKILKKLGIETVKDLLWWLPRDYEDRRRIVPLSSIVADRKVTIRAKLQNFSVKKVKEY
HHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCEEEEEEEHHCCCHHHHHHH
VIISAVVSDGFGQIILKWFNQEYITDRLIKEREYLITGIPKKTPFGPYEMNSPEIEEITG
HHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHC
RVPREILPLYSLSAGISQKVMRKIVRKNITNVRLLKEFIPAEVIKERNLLPRHHAMYTVH
CCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEE
FPKSLYELKESRRRLAYEELFLFEVAVLYNREKLKTTKGGISKSISGKLAERFIDSLNFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEE
LTGDQMRAFEEIREDMKAPTPMNRLLQGDVGSGKTVVAELAIIDNFEAGYQSAMMVPTTV
EECHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEEEHHHCCCCHHHHCCHHHHHHH
LATQQHQKLVKDLEPLGIKVELLVGSQKKSQQEEIKKRIAIGEVDVVVGTHALIQENVKF
HHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCE
KDLGLVIIDEQHRFGVKQREALMNKGALVDTLVMTATPIPRTLALTAYGDLDISTITEMP
EECCEEEECCHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHCCC
PGRAPIRTMLISEKRLPELYAFIRDEVNHGHQAFFIYPLIEESEQMDLKAATDEAERLQK
CCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHCCHHHHHHH
EVFPDIGVELLHGRMSDEEKNRIMHRFKNKEAMILVSTSVVEVGIDIPTATVMVIEHPER
HHCHHHCHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCC
FGLAQLHQLRGRVGRSSLKSYCMLVLNSNISGEALDRLRKFAGTQNGFKLAEIDLSLRGP
CCHHHHHHHHHHHCHHHHHHHEEHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCC
GEFMGTRQHGLPDFLVADIVKDSELLIMARNDAMELLKRDPNLEKHNRIIEEIKERFGEN
HHHCCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
ISLIEVG
CEEEEEC
>Mature Secondary Structure
MLLEDYLDRCEKLLEKFLSGKVDTIELIQEIKRGAELLEPEELSANEGLKDYIGKFVSYY
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LPLASLPNERQHLRVKNGFTMINKLRGKFLLTSFDESTSAIPVKVSSLNFDIKYAKGVGE
HHHHCCCCCHHHEEECCCHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEEEEEECCCCCH
RRAKILKKLGIETVKDLLWWLPRDYEDRRRIVPLSSIVADRKVTIRAKLQNFSVKKVKEY
HHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCEEEEEEEHHCCCHHHHHHH
VIISAVVSDGFGQIILKWFNQEYITDRLIKEREYLITGIPKKTPFGPYEMNSPEIEEITG
HHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHC
RVPREILPLYSLSAGISQKVMRKIVRKNITNVRLLKEFIPAEVIKERNLLPRHHAMYTVH
CCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEE
FPKSLYELKESRRRLAYEELFLFEVAVLYNREKLKTTKGGISKSISGKLAERFIDSLNFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEE
LTGDQMRAFEEIREDMKAPTPMNRLLQGDVGSGKTVVAELAIIDNFEAGYQSAMMVPTTV
EECHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEEEHHHCCCCHHHHCCHHHHHHH
LATQQHQKLVKDLEPLGIKVELLVGSQKKSQQEEIKKRIAIGEVDVVVGTHALIQENVKF
HHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCE
KDLGLVIIDEQHRFGVKQREALMNKGALVDTLVMTATPIPRTLALTAYGDLDISTITEMP
EECCEEEECCHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHCCC
PGRAPIRTMLISEKRLPELYAFIRDEVNHGHQAFFIYPLIEESEQMDLKAATDEAERLQK
CCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHCCHHHHHHH
EVFPDIGVELLHGRMSDEEKNRIMHRFKNKEAMILVSTSVVEVGIDIPTATVMVIEHPER
HHCHHHCHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCC
FGLAQLHQLRGRVGRSSLKSYCMLVLNSNISGEALDRLRKFAGTQNGFKLAEIDLSLRGP
CCHHHHHHHHHHHCHHHHHHHEEHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCC
GEFMGTRQHGLPDFLVADIVKDSELLIMARNDAMELLKRDPNLEKHNRIIEEIKERFGEN
HHHCCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
ISLIEVG
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]