Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 238925625

GI number: 238925625

Start: 3115706

End: 3117286

Strand: Reverse

Name: dacB [H]

Synonym: EUBREC_3281

Alternate gene names: 238925625

Gene position: 3117286-3115706 (Counterclockwise)

Preceding gene: 238925627

Following gene: 238925624

Centisome position: 90.36

GC content: 43.14

Gene sequence:

>1581_bases
GTGGGTGTCAAAATAAAATGGAAAAATACGATGCACATGCCCTTGATAACATGTTTTTCATACATTATAATTAGAAATAG
TACATTTTTGGTTATAGTACTGAACAGCTACCCAATTTTAGAATTAATAGGAACAGATATGAAGCATAAAATTATAAATT
TAAAAAAATCAGGAATACTAATAATGGCAGTGTTTGCTATGGTCACTACGCTTTGTGCGAGACCGCTTATGGCATTTGCG
GATGGTGATGACACGTACTGGCCGGAGGGACCGCAGATAAATTCACCGTGCGCCGTGGTGATGGAGGTCAATACAGGTAC
GGTGCTATATGAGAAAAATTCACACGAAAAGCATTATCCTGCAAGTATCACAAAGATCCTCACCACATATCTGACTATAC
TCAACTGCAAGATGGATGAGAAGGTTACATTCTCAAAAGAGGCGGTGAAGGAGAGCAGTGATGGTTCGTCGAGCATCAAG
AGGGATGTGGGCGAGGAGATGACCATGGAGCAGACACTATATGGAGTTATGCTTGAGTCGGCAAACGAGTGCGCATATGC
TGCAGCAGAGCATACAGGCAAAAAGCTTGGGGGAGATTACAGCACATTTATTGATCTGATGAACAAGGAAGCCAAAGAAT
TAGGCTGTACAGATACACATTTCAACAATGCAAATGGTCTGCCCGATGAAAATCACTGGACAAGCGCATACGATATGGGG
CTTATCTCGTGTGCAGCATACAGAAATGACGAGTTTCGAAAGATTACAGGCACAAAGACCTATATCATACCACCTACAAA
CAAGCATACTGAGGATACCCCGCTGTACAATCATCATGCGATGCTGCATCCATTTAAGTCGTATTCACAGTTTGTAAATC
ATGATTGTACCGGCGGCAAGACCGGCTACACTTCAGTGGCTAACAGCACGCTTGTCACTTATGCGGAGAAGGATGGCCTG
ACGCTGTGCGTGGTTATTATGAATGCACAGTCGCCTGACCAGTATGCAGACACAAACACGCTGCTCAATTACTATTTCAG
TAACTTCAAGGCACTAAGCATAGCTGAAAATGAAAACAGCGCAGCTGCTGACAATACGCCGGATCTGGGCGTGATGAATG
AGCACGGCATGTATGCAAAGGTATCGGAAAACTATGTTGTTTTGCCAAACAATGCGGATTTTTCTAGCATTAAAAGGGAA
GCTGCTGACATGAAGTCCGACAACAAGGATGCTCTTGCTACGGTCAGATACAAGTATGGTGATCATGTGGTAGGCTGTGT
GGATCTGCTGAAATCAGGAGCAAAGGTCGATCTGTCGTACTTTGATGAGAACAGCAGACAGAACGTGGAAAGTGATATAA
TAAGGATAAAGCCGGTATATTTTCTCTGGGTGCTCGTACTTATCGCAGTGATTATTCTGAGCATTTTCATGATTAGAAGA
ATATCAGACAATATATATGTGTTGAAACACAAATGGAGCATTAAGAAGGAGCAGCGCGAGCGCTTCAGGGAGCACAAGCT
TGAGAGAAAACAGCGCAGAAGGAGAGACAATCTGAAGTTCAGCGGTCGACATAAATATTAA

Upstream 100 bases:

>100_bases
TGTTGTCGATGACTTCACCGGCGCCAGGGATATTTCTAAGTCTCATGTAAATCTCCTTATAAAAACGTAAAATTTCATAT
AGATAATACCCTACAATTGT

Downstream 100 bases:

>100_bases
TACATTTTTTATTATGACATTATAAATGGAGGCAGAAATGAGTGATGAAAGATGCGCCCTCGTGCTTGAGGGTGGAGCCA
TGAGAGGTATGTATACAGCC

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MGVKIKWKNTMHMPLITCFSYIIIRNSTFLVIVLNSYPILELIGTDMKHKIINLKKSGILIMAVFAMVTTLCARPLMAFA
DGDDTYWPEGPQINSPCAVVMEVNTGTVLYEKNSHEKHYPASITKILTTYLTILNCKMDEKVTFSKEAVKESSDGSSSIK
RDVGEEMTMEQTLYGVMLESANECAYAAAEHTGKKLGGDYSTFIDLMNKEAKELGCTDTHFNNANGLPDENHWTSAYDMG
LISCAAYRNDEFRKITGTKTYIIPPTNKHTEDTPLYNHHAMLHPFKSYSQFVNHDCTGGKTGYTSVANSTLVTYAEKDGL
TLCVVIMNAQSPDQYADTNTLLNYYFSNFKALSIAENENSAAADNTPDLGVMNEHGMYAKVSENYVVLPNNADFSSIKRE
AADMKSDNKDALATVRYKYGDHVVGCVDLLKSGAKVDLSYFDENSRQNVESDIIRIKPVYFLWVLVLIAVIILSIFMIRR
ISDNIYVLKHKWSIKKEQRERFREHKLERKQRRRRDNLKFSGRHKY

Sequences:

>Translated_526_residues
MGVKIKWKNTMHMPLITCFSYIIIRNSTFLVIVLNSYPILELIGTDMKHKIINLKKSGILIMAVFAMVTTLCARPLMAFA
DGDDTYWPEGPQINSPCAVVMEVNTGTVLYEKNSHEKHYPASITKILTTYLTILNCKMDEKVTFSKEAVKESSDGSSSIK
RDVGEEMTMEQTLYGVMLESANECAYAAAEHTGKKLGGDYSTFIDLMNKEAKELGCTDTHFNNANGLPDENHWTSAYDMG
LISCAAYRNDEFRKITGTKTYIIPPTNKHTEDTPLYNHHAMLHPFKSYSQFVNHDCTGGKTGYTSVANSTLVTYAEKDGL
TLCVVIMNAQSPDQYADTNTLLNYYFSNFKALSIAENENSAAADNTPDLGVMNEHGMYAKVSENYVVLPNNADFSSIKRE
AADMKSDNKDALATVRYKYGDHVVGCVDLLKSGAKVDLSYFDENSRQNVESDIIRIKPVYFLWVLVLIAVIILSIFMIRR
ISDNIYVLKHKWSIKKEQRERFREHKLERKQRRRRDNLKFSGRHKY
>Mature_525_residues
GVKIKWKNTMHMPLITCFSYIIIRNSTFLVIVLNSYPILELIGTDMKHKIINLKKSGILIMAVFAMVTTLCARPLMAFAD
GDDTYWPEGPQINSPCAVVMEVNTGTVLYEKNSHEKHYPASITKILTTYLTILNCKMDEKVTFSKEAVKESSDGSSSIKR
DVGEEMTMEQTLYGVMLESANECAYAAAEHTGKKLGGDYSTFIDLMNKEAKELGCTDTHFNNANGLPDENHWTSAYDMGL
ISCAAYRNDEFRKITGTKTYIIPPTNKHTEDTPLYNHHAMLHPFKSYSQFVNHDCTGGKTGYTSVANSTLVTYAEKDGLT
LCVVIMNAQSPDQYADTNTLLNYYFSNFKALSIAENENSAAADNTPDLGVMNEHGMYAKVSENYVVLPNNADFSSIKREA
ADMKSDNKDALATVRYKYGDHVVGCVDLLKSGAKVDLSYFDENSRQNVESDIIRIKPVYFLWVLVLIAVIILSIFMIRRI
SDNIYVLKHKWSIKKEQRERFREHKLERKQRRRRDNLKFSGRHKY

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=308, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787062, Length=275, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87082022, Length=305, Percent_Identity=26.5573770491803, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87082054, Length=244, Percent_Identity=25, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 59631; Mature: 59500

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVKIKWKNTMHMPLITCFSYIIIRNSTFLVIVLNSYPILELIGTDMKHKIINLKKSGIL
CCEEEEECCCCCCHHHHHHHHHHEECCEEEEEEECCCCCHHHHCCCHHHHHHEECCCCHH
IMAVFAMVTTLCARPLMAFADGDDTYWPEGPQINSPCAVVMEVNTGTVLYEKNSHEKHYP
HHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCC
ASITKILTTYLTILNCKMDEKVTFSKEAVKESSDGSSSIKRDVGEEMTMEQTLYGVMLES
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
ANECAYAAAEHTGKKLGGDYSTFIDLMNKEAKELGCTDTHFNNANGLPDENHWTSAYDMG
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
LISCAAYRNDEFRKITGTKTYIIPPTNKHTEDTPLYNHHAMLHPFKSYSQFVNHDCTGGK
HHHHHHHCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCC
TGYTSVANSTLVTYAEKDGLTLCVVIMNAQSPDQYADTNTLLNYYFSNFKALSIAENENS
CCCHHHCCCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEECCCCC
AAADNTPDLGVMNEHGMYAKVSENYVVLPNNADFSSIKREAADMKSDNKDALATVRYKYG
CCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEECC
DHVVGCVDLLKSGAKVDLSYFDENSRQNVESDIIRIKPVYFLWVLVLIAVIILSIFMIRR
CHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHEEECHHHHHHHHHHHHHHHHHHHHHHH
ISDNIYVLKHKWSIKKEQRERFREHKLERKQRRRRDNLKFSGRHKY
HCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GVKIKWKNTMHMPLITCFSYIIIRNSTFLVIVLNSYPILELIGTDMKHKIINLKKSGIL
CEEEEECCCCCCHHHHHHHHHHEECCEEEEEEECCCCCHHHHCCCHHHHHHEECCCCHH
IMAVFAMVTTLCARPLMAFADGDDTYWPEGPQINSPCAVVMEVNTGTVLYEKNSHEKHYP
HHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCC
ASITKILTTYLTILNCKMDEKVTFSKEAVKESSDGSSSIKRDVGEEMTMEQTLYGVMLES
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
ANECAYAAAEHTGKKLGGDYSTFIDLMNKEAKELGCTDTHFNNANGLPDENHWTSAYDMG
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
LISCAAYRNDEFRKITGTKTYIIPPTNKHTEDTPLYNHHAMLHPFKSYSQFVNHDCTGGK
HHHHHHHCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCC
TGYTSVANSTLVTYAEKDGLTLCVVIMNAQSPDQYADTNTLLNYYFSNFKALSIAENENS
CCCHHHCCCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEECCCCC
AAADNTPDLGVMNEHGMYAKVSENYVVLPNNADFSSIKREAADMKSDNKDALATVRYKYG
CCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEECC
DHVVGCVDLLKSGAKVDLSYFDENSRQNVESDIIRIKPVYFLWVLVLIAVIILSIFMIRR
CHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHEEECHHHHHHHHHHHHHHHHHHHHHHH
ISDNIYVLKHKWSIKKEQRERFREHKLERKQRRRRDNLKFSGRHKY
HCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]