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The map label for this gene is mleN [H]

Identifier: 238925448

GI number: 238925448

Start: 2963570

End: 2964928

Strand: Reverse

Name: mleN [H]

Synonym: EUBREC_3103

Alternate gene names: 238925448

Gene position: 2964928-2963570 (Counterclockwise)

Preceding gene: 238925449

Following gene: 238925445

Centisome position: 85.95

GC content: 44.52

Gene sequence:

>1359_bases
ATGATGAAAATTGCTAAAAAAATGTCAAGGAAACATATAAACGTGAAAAAACAAAATCCAATAGCCCTGTTACCAATAGC
AGTATTTCTTGTACTGTATCTGGGATTGGGAATACTTTTTGAATATGTAATGAAAATTCCGATGGGATTTTATAATGTGC
CCATCATAATAGCATTTCTCGTGGCAATACTGGTGGCGTGTCTGCAGAACCGTAGTGTGAAATTCGACCAAAAGCTAGAG
ATAATGGCACAGGGACTGGCTGACAAAAATATCATAACAATGCTGCTCATTTTCCTTACCGCGGGAGCATTCGTGGGTGT
AGTAGGTCGAAGCAGTGCGGAGAGCGTGGCATATTTCATGCTGTCACTGATACCGGCAAGGTTCGCGGTGATGGTGCTTT
TTGTTGTGGCATGCTTTGTGTCTATTGCGATGGGGACATCGGTTGGAACCATAACACTGATTGTGCCGATAGCGGCAGCA
GTATCGGATGCATCGGGATTTGGTCTGCCGCTTTGTATAGGCTCCGTAATGGGTGGCGCGATGTTCGGAGACAATCTTTC
CTTTATATCAGATACGACCATTGCCGCATGTAACGGACAGGGTTGTCAGATGAAGGACAAATTCAGGGAGAATTTTTTTA
TTGCACTTCCGGCGGCAGTAATGACGCTGGTTGTTATAGCAATTCTGTCATTCAGGACAGATATCGCGGGCGCTGTATCG
CAGGAATATCATCTGTTGCAGATAGTACCGTATATTCTCGTGCTGATCGGTGGAATTGCCGGAATAAATGTATTTGTAGT
GCTTATAATAGGAATCATATCGGGAACGGTCATAATGCTCGCAACAGGAAATACAGCACCATACGATCTGCTTACCAATA
TGGGAAGTGGAGCGTCAGGCATGTTTGAAACCTGTATGGTTGCAGTGCTTGTGGCAGCCATGTGTGCGCTGATTCGTGAA
TATGGTGGCTTTGATGCCCTGCTTTCGGGTATTTACAGGACATTCAAGGGCAAAAGAGGTGGACTGCTCGGTATGGGACT
TCTGGTAGGACTTATCGATATTGCCACTGCCAACAACACAGTTGCTATAGTTATGGCAAATCCGATAGCAAAGGAAATGG
CTCAGAAATATGATATAACCCCACGTAAAACAGCGTCTATACTGGATACATTTTCGTGCATTTTTCAGGGGATGATACCA
TATGGAGCGCAGATGCTAGTGGCTATATCGGCAGTACACGAGCTGGGGCATGATGTGTCAGCCTTTAATATACTGCCGTA
TCTGTTGTATCCGATGTTTCTGCTGGTAAGCTCGCTGGTGGCGGTTTTTGTTGTTGAAAATGGGAGAAAATTTAATTGA

Upstream 100 bases:

>100_bases
GCGCGAAACGCTTGCGGCTCACAACTGTGAAGGTACTGAACAGTTACATAAAATTATAATAAATAGCATATTATTATTGA
CATACTAATGATGATTACTT

Downstream 100 bases:

>100_bases
AGATGAAGTTTAAAGCAAAAGAATAATGAGGTTATAAATGAAAATGCCGGATAGAAATATGATATTTCATCCGGCATTTA
TTATGTGAAATTTAGGCTGC

Product: malate-H+/Na+-lactate antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MMKIAKKMSRKHINVKKQNPIALLPIAVFLVLYLGLGILFEYVMKIPMGFYNVPIIIAFLVAILVACLQNRSVKFDQKLE
IMAQGLADKNIITMLLIFLTAGAFVGVVGRSSAESVAYFMLSLIPARFAVMVLFVVACFVSIAMGTSVGTITLIVPIAAA
VSDASGFGLPLCIGSVMGGAMFGDNLSFISDTTIAACNGQGCQMKDKFRENFFIALPAAVMTLVVIAILSFRTDIAGAVS
QEYHLLQIVPYILVLIGGIAGINVFVVLIIGIISGTVIMLATGNTAPYDLLTNMGSGASGMFETCMVAVLVAAMCALIRE
YGGFDALLSGIYRTFKGKRGGLLGMGLLVGLIDIATANNTVAIVMANPIAKEMAQKYDITPRKTASILDTFSCIFQGMIP
YGAQMLVAISAVHELGHDVSAFNILPYLLYPMFLLVSSLVAVFVVENGRKFN

Sequences:

>Translated_452_residues
MMKIAKKMSRKHINVKKQNPIALLPIAVFLVLYLGLGILFEYVMKIPMGFYNVPIIIAFLVAILVACLQNRSVKFDQKLE
IMAQGLADKNIITMLLIFLTAGAFVGVVGRSSAESVAYFMLSLIPARFAVMVLFVVACFVSIAMGTSVGTITLIVPIAAA
VSDASGFGLPLCIGSVMGGAMFGDNLSFISDTTIAACNGQGCQMKDKFRENFFIALPAAVMTLVVIAILSFRTDIAGAVS
QEYHLLQIVPYILVLIGGIAGINVFVVLIIGIISGTVIMLATGNTAPYDLLTNMGSGASGMFETCMVAVLVAAMCALIRE
YGGFDALLSGIYRTFKGKRGGLLGMGLLVGLIDIATANNTVAIVMANPIAKEMAQKYDITPRKTASILDTFSCIFQGMIP
YGAQMLVAISAVHELGHDVSAFNILPYLLYPMFLLVSSLVAVFVVENGRKFN
>Mature_452_residues
MMKIAKKMSRKHINVKKQNPIALLPIAVFLVLYLGLGILFEYVMKIPMGFYNVPIIIAFLVAILVACLQNRSVKFDQKLE
IMAQGLADKNIITMLLIFLTAGAFVGVVGRSSAESVAYFMLSLIPARFAVMVLFVVACFVSIAMGTSVGTITLIVPIAAA
VSDASGFGLPLCIGSVMGGAMFGDNLSFISDTTIAACNGQGCQMKDKFRENFFIALPAAVMTLVVIAILSFRTDIAGAVS
QEYHLLQIVPYILVLIGGIAGINVFVVLIIGIISGTVIMLATGNTAPYDLLTNMGSGASGMFETCMVAVLVAAMCALIRE
YGGFDALLSGIYRTFKGKRGGLLGMGLLVGLIDIATANNTVAIVMANPIAKEMAQKYDITPRKTASILDTFSCIFQGMIP
YGAQMLVAISAVHELGHDVSAFNILPYLLYPMFLLVSSLVAVFVVENGRKFN

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 48232; Mature: 48232

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]