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The map label for this gene is opuE [H]

Identifier: 238924748

GI number: 238924748

Start: 2265337

End: 2266788

Strand: Direct

Name: opuE [H]

Synonym: EUBREC_2399

Alternate gene names: 238924748

Gene position: 2265337-2266788 (Clockwise)

Preceding gene: 238924747

Following gene: 238924751

Centisome position: 65.67

GC content: 45.52

Gene sequence:

>1452_bases
ATGAAAACATCAGAAATTATTGTATTTATCGTCTATCTGCTGTGCATGCTGGGTATCGGTATCTTCTTCTTCGTGAAAAA
CAGAAACGGCGGAGAAAAGACCTACTTCCTCGGCGGACGTGAGATGGGACCATGGGTTGCGGCGCTGTCAGCCGGAGCCT
CAGACATGAGTGCATGGGTACTCATGGGACTTCCTACTTCTATCTATGCACTGGGCTTAGGTCAGGTATGGATTCCTGTA
GGACTTGCAATCGGCTACACTATAAGCTGGCTGCTAGAGGCCCCTAGACTGAGAAAATTCTCGATTGTAGCAAACGACTC
CATCACCATTCCGCAGTATCTGACCAACAGATTCCTGTCCAAATCACTTGCACTGCAGGTGATTTGTGCAATTGTGTTTT
TGGTTGCATACACTATTTATGCGGCATCGAGCGTAAAGGCATGTGGTACTCTTTTCCACACTGTAATAGGAATGGATCCA
CAGATTGCCATGTATCTTGCAGCAGTTGTCATCGTAGGCTATACCTTCCTCGGCGGCTTCTCGGCTGTATGCTGGACCGA
TTTTTTCCAGGGCATGCTCGTACTTGGTTCTATGTTTATAGCACCGATTTTTGCAGCATTTATGCTTGATACATCATCTA
TGTCATCACTCCCGGGCGGTTACTTAAATCCATTCTCAAGCTGGCAGGATATCGTGTCAGGACTGGCATGGGGACTTGGT
TACTTTGGTATGCCACATATCATTATCAGATTCATGTCCATCAAATCACAGAAGGATTTGAAAAAATCAGCAAAAATCGG
AATCACATGGACCTTGCTTATTGTATTTTTTGCAACCTTAATCGGAGTAATCGGAAGACTTTTCCTTGGCTTTGACGAAT
CAATCAATGAAAACTCTCTTGTTTTCATCCAGATTGTAAGAAAGATTTTCCCGGGTGTTATCTCAGGTATACTCCTCTCT
GCAGTGCTTGCTGCTTCCATGAGCACCGCAGACAGCCAGCTGCTTGCTGCTTCATCTGCATTCTCGAGTGATGTGTACAA
GCCTGTCATCAGAAAAGGCAAGGCAAGTGATAAAGAGATGATGTGGACCGGCCGTATTGTCGTTCTTGGAATCTCTGTTG
CAGCACTCATCATCGCATCAAACCCTAATGCCGGCTCTATCATGGCACTTGTTTCCAATGCATGGGGTATCTTTGGAGCT
GCCTTTGGACCTGCAATCATGCTCAGCCTCTTCTGGAAGCGCTTTAACTTCAAGGGTGCTGTTGCAGGAATCATAACAGG
TGCTGCCGTTGATATATTGTGGTATGCATTCCTCACATCAACCGGAATCTACGAGCTGCTGCCGGGATTTGTTTTAGGAC
TTATCGCAGCCGTTGTTGTGACACTTTGCACAAATGAGCCTTCAAAAGAGGTTTATGAGCTCTTCGATAAGGCTCAAAAA
TTTGATGAGTAA

Upstream 100 bases:

>100_bases
ATGTCAACGCAGTGTATTCGTCAAGAGAAAGTTTTTCTTGCCTTTATCACGCTAACGTGGTAGCATGGTTACTAAATAAA
CTATTAAAGAGGTACTACAA

Downstream 100 bases:

>100_bases
TTGAAATAATTAATCATTTAAGAACATAAGTAAAACCGTCGTACCCTGCCATTTACGGCCCGGTACGACGGTTTTATTTT
TCTTATGCTTTATGTTGTGC

Product: sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKTSEIIVFIVYLLCMLGIGIFFFVKNRNGGEKTYFLGGREMGPWVAALSAGASDMSAWVLMGLPTSIYALGLGQVWIPV
GLAIGYTISWLLEAPRLRKFSIVANDSITIPQYLTNRFLSKSLALQVICAIVFLVAYTIYAASSVKACGTLFHTVIGMDP
QIAMYLAAVVIVGYTFLGGFSAVCWTDFFQGMLVLGSMFIAPIFAAFMLDTSSMSSLPGGYLNPFSSWQDIVSGLAWGLG
YFGMPHIIIRFMSIKSQKDLKKSAKIGITWTLLIVFFATLIGVIGRLFLGFDESINENSLVFIQIVRKIFPGVISGILLS
AVLAASMSTADSQLLAASSAFSSDVYKPVIRKGKASDKEMMWTGRIVVLGISVAALIIASNPNAGSIMALVSNAWGIFGA
AFGPAIMLSLFWKRFNFKGAVAGIITGAAVDILWYAFLTSTGIYELLPGFVLGLIAAVVVTLCTNEPSKEVYELFDKAQK
FDE

Sequences:

>Translated_483_residues
MKTSEIIVFIVYLLCMLGIGIFFFVKNRNGGEKTYFLGGREMGPWVAALSAGASDMSAWVLMGLPTSIYALGLGQVWIPV
GLAIGYTISWLLEAPRLRKFSIVANDSITIPQYLTNRFLSKSLALQVICAIVFLVAYTIYAASSVKACGTLFHTVIGMDP
QIAMYLAAVVIVGYTFLGGFSAVCWTDFFQGMLVLGSMFIAPIFAAFMLDTSSMSSLPGGYLNPFSSWQDIVSGLAWGLG
YFGMPHIIIRFMSIKSQKDLKKSAKIGITWTLLIVFFATLIGVIGRLFLGFDESINENSLVFIQIVRKIFPGVISGILLS
AVLAASMSTADSQLLAASSAFSSDVYKPVIRKGKASDKEMMWTGRIVVLGISVAALIIASNPNAGSIMALVSNAWGIFGA
AFGPAIMLSLFWKRFNFKGAVAGIITGAAVDILWYAFLTSTGIYELLPGFVLGLIAAVVVTLCTNEPSKEVYELFDKAQK
FDE
>Mature_483_residues
MKTSEIIVFIVYLLCMLGIGIFFFVKNRNGGEKTYFLGGREMGPWVAALSAGASDMSAWVLMGLPTSIYALGLGQVWIPV
GLAIGYTISWLLEAPRLRKFSIVANDSITIPQYLTNRFLSKSLALQVICAIVFLVAYTIYAASSVKACGTLFHTVIGMDP
QIAMYLAAVVIVGYTFLGGFSAVCWTDFFQGMLVLGSMFIAPIFAAFMLDTSSMSSLPGGYLNPFSSWQDIVSGLAWGLG
YFGMPHIIIRFMSIKSQKDLKKSAKIGITWTLLIVFFATLIGVIGRLFLGFDESINENSLVFIQIVRKIFPGVISGILLS
AVLAASMSTADSQLLAASSAFSSDVYKPVIRKGKASDKEMMWTGRIVVLGISVAALIIASNPNAGSIMALVSNAWGIFGA
AFGPAIMLSLFWKRFNFKGAVAGIITGAAVDILWYAFLTSTGIYELLPGFVLGLIAAVVVTLCTNEPSKEVYELFDKAQK
FDE

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=492, Percent_Identity=40.4471544715447, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI14140236, Length=492, Percent_Identity=23.3739837398374, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4507031, Length=473, Percent_Identity=24.7357293868922, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI11141885, Length=525, Percent_Identity=24.5714285714286, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI4507035, Length=371, Percent_Identity=25.6064690026954, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI110835708, Length=551, Percent_Identity=25.7713248638838, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI17941285, Length=470, Percent_Identity=24.0425531914894, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI206597483, Length=464, Percent_Identity=24.5689655172414, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI157671931, Length=456, Percent_Identity=25.219298245614, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI167466278, Length=495, Percent_Identity=25.2525252525253, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4507033, Length=481, Percent_Identity=22.8690228690229, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI206597487, Length=493, Percent_Identity=22.920892494929, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI109659836, Length=503, Percent_Identity=20.2783300198807, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1787251, Length=491, Percent_Identity=39.3075356415479, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI87082237, Length=463, Percent_Identity=27.8617710583153, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790503, Length=439, Percent_Identity=23.6902050113895, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790113, Length=449, Percent_Identity=22.9398663697105, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539284, Length=454, Percent_Identity=24.6696035242291, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648033, Length=446, Percent_Identity=25.3363228699552, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356865, Length=446, Percent_Identity=25.3363228699552, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24651739, Length=479, Percent_Identity=24.8434237995825, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221459584, Length=402, Percent_Identity=23.8805970149254, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645928, Length=371, Percent_Identity=24.2587601078167, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI221459586, Length=425, Percent_Identity=24.4705882352941, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI221459588, Length=435, Percent_Identity=25.5172413793103, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24650192, Length=373, Percent_Identity=23.8605898123324, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24651741, Length=458, Percent_Identity=23.5807860262009, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362918, Length=460, Percent_Identity=24.5652173913043, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI221459582, Length=388, Percent_Identity=25.5154639175258, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641117, Length=458, Percent_Identity=24.0174672489083, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 52177; Mature: 52177

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCC
>Mature Secondary Structure
MKTSEIIVFIVYLLCMLGIGIFFFVKNRNGGEKTYFLGGREMGPWVAALSAGASDMSAWV
CCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHH
LMGLPTSIYALGLGQVWIPVGLAIGYTISWLLEAPRLRKFSIVANDSITIPQYLTNRFLS
HHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
KSLALQVICAIVFLVAYTIYAASSVKACGTLFHTVIGMDPQIAMYLAAVVIVGYTFLGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SAVCWTDFFQGMLVLGSMFIAPIFAAFMLDTSSMSSLPGGYLNPFSSWQDIVSGLAWGLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
YFGMPHIIIRFMSIKSQKDLKKSAKIGITWTLLIVFFATLIGVIGRLFLGFDESINENSL
HCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VFIQIVRKIFPGVISGILLSAVLAASMSTADSQLLAASSAFSSDVYKPVIRKGKASDKEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MWTGRIVVLGISVAALIIASNPNAGSIMALVSNAWGIFGAAFGPAIMLSLFWKRFNFKGA
EEECCHHEEHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VAGIITGAAVDILWYAFLTSTGIYELLPGFVLGLIAAVVVTLCTNEPSKEVYELFDKAQK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FDE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]