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The map label for this gene is arsB [C]

Identifier: 238922387

GI number: 238922387

Start: 591163

End: 592521

Strand: Reverse

Name: arsB [C]

Synonym: EUBELI_20623

Alternate gene names: 238922387

Gene position: 592521-591163 (Counterclockwise)

Preceding gene: 238922388

Following gene: 238922386

Centisome position: 94.54

GC content: 38.48

Gene sequence:

>1359_bases
ATGTTAGCAGAGCAGGTATTGGCATTAATTATTTTCATTTCTATGTTTGTATTGATAGTTCTTGATAAGATAGAGAGGCA
TTATGTGACGCTTGGATGTGGGGCATTGACGCTGATTGGGGTATTTGGAATTATCATGAGGTCGCCGGAGGCGGTTATTA
GTACTCTTAATTTAAGAAGCATTTTTACATCAGGTTTCTGGTATCAGTCCGGAGCTGCTGAAGAGGCAGTAACAGGGATT
AACTGGTCGACCATTATATTTATTGCGGGTATGATGATTATGGTTGAGGTTATGGCTGCCGCCGGATTTTTCAGATGGCT
GTGTATGCTTCTGGCTAAGGCTGTACACTATCGTACAAGAGCATTGTTTATTACATTTATGATAATGTCTTTTGGATTGG
CTATGTTTATAGACAGTATAACGGTTATATTGTTTCTTGCAGCTGTATCTATTGAGCTTGCCAAGCTGCTAAAGTTTAAC
CCAGTGCCAATGATTTTATCAGAGATATTCTGTGCTAATCTGGGCGGTTCGGCAACAATGTGCGGGGATCCACCTAATAT
AATTATTGGTACGTCACTTAATTTTTCATTTTCAACATTTATTACGCATACAGGACTGATTGCAGTAATCAGTCTGGTAT
TTATTGTGGTATATTTTCTTATAGCATATAGAAAAGAACTGGAATCATCTCCAGATATTGATAAACTGGCTGAATCTATG
CCAAGTCCGGCAGAGACTATTACTAACAGAAGAGATTTTGCATTAAGCTGTGTTATATTCATGTGTGCAGTTGTAATGCT
TGTAACGCATGCGCTGACAGGACTTACGGTTGCATTTATAGGACTTGTTATAGCAATATTTTCACTTATTGTATCAGGCA
GGAAGGCTTTACATATGTTGAAGTCTATTGATTATAAAACAATTTTGTTCTTTTTAGGACTGTTTATTGTTGTAGGTGGA
CTTGAACAGACGGGCATATTAGAGATTATTGCTGCATTTATAGGTCGTGTGAGCAAGGGTAATCTTAAGCTTATGGTTGC
AATTATTATTATTATTTCAGCATGTGCAAGTGCACTTATTGATAATATTCCTTTTGCAGCAACTATGGTACCGGTTATTA
AGAGTCTGGCTGCGACACAAGGGGTTGACCTTTCTGTTCTTGCGTGGGCACTTTCTATGGGAACTGATGTAGGTGGAAGT
GCAACACCTATAGGTGCATCTGCTAATGTTGTCGGAACATCAGTTGCAGCTAAGAATGGTTATATGATTGGTTGGGGTAA
GTATTGCCGCACAGCTGTTCCGGCAACTATAATAGTTGTGCTTATATCTATGGTGTGCATATTTGTAAGGTATTGTTAA

Upstream 100 bases:

>100_bases
GGACAGAATAAATGGAAAAATGTTGCTATTAGTTATATATGTTTGTATAATACAGTTAAAAGTTTGTTATTAATTTAACA
TGTGATGGGGGTTATTTAAT

Downstream 100 bases:

>100_bases
TTCTTCGTGCACAAAATGTGTACAGAAATGGGGGTTTTATGGGAGAACAGTTGATTATTTCTATAAGTCGTGAATATGGA
AGTGGAGGACATGAGATAGC

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MLAEQVLALIIFISMFVLIVLDKIERHYVTLGCGALTLIGVFGIIMRSPEAVISTLNLRSIFTSGFWYQSGAAEEAVTGI
NWSTIIFIAGMMIMVEVMAAAGFFRWLCMLLAKAVHYRTRALFITFMIMSFGLAMFIDSITVILFLAAVSIELAKLLKFN
PVPMILSEIFCANLGGSATMCGDPPNIIIGTSLNFSFSTFITHTGLIAVISLVFIVVYFLIAYRKELESSPDIDKLAESM
PSPAETITNRRDFALSCVIFMCAVVMLVTHALTGLTVAFIGLVIAIFSLIVSGRKALHMLKSIDYKTILFFLGLFIVVGG
LEQTGILEIIAAFIGRVSKGNLKLMVAIIIIISACASALIDNIPFAATMVPVIKSLAATQGVDLSVLAWALSMGTDVGGS
ATPIGASANVVGTSVAAKNGYMIGWGKYCRTAVPATIIVVLISMVCIFVRYC

Sequences:

>Translated_452_residues
MLAEQVLALIIFISMFVLIVLDKIERHYVTLGCGALTLIGVFGIIMRSPEAVISTLNLRSIFTSGFWYQSGAAEEAVTGI
NWSTIIFIAGMMIMVEVMAAAGFFRWLCMLLAKAVHYRTRALFITFMIMSFGLAMFIDSITVILFLAAVSIELAKLLKFN
PVPMILSEIFCANLGGSATMCGDPPNIIIGTSLNFSFSTFITHTGLIAVISLVFIVVYFLIAYRKELESSPDIDKLAESM
PSPAETITNRRDFALSCVIFMCAVVMLVTHALTGLTVAFIGLVIAIFSLIVSGRKALHMLKSIDYKTILFFLGLFIVVGG
LEQTGILEIIAAFIGRVSKGNLKLMVAIIIIISACASALIDNIPFAATMVPVIKSLAATQGVDLSVLAWALSMGTDVGGS
ATPIGASANVVGTSVAAKNGYMIGWGKYCRTAVPATIIVVLISMVCIFVRYC
>Mature_452_residues
MLAEQVLALIIFISMFVLIVLDKIERHYVTLGCGALTLIGVFGIIMRSPEAVISTLNLRSIFTSGFWYQSGAAEEAVTGI
NWSTIIFIAGMMIMVEVMAAAGFFRWLCMLLAKAVHYRTRALFITFMIMSFGLAMFIDSITVILFLAAVSIELAKLLKFN
PVPMILSEIFCANLGGSATMCGDPPNIIIGTSLNFSFSTFITHTGLIAVISLVFIVVYFLIAYRKELESSPDIDKLAESM
PSPAETITNRRDFALSCVIFMCAVVMLVTHALTGLTVAFIGLVIAIFSLIVSGRKALHMLKSIDYKTILFFLGLFIVVGG
LEQTGILEIIAAFIGRVSKGNLKLMVAIIIIISACASALIDNIPFAATMVPVIKSLAATQGVDLSVLAWALSMGTDVGGS
ATPIGASANVVGTSVAAKNGYMIGWGKYCRTAVPATIIVVLISMVCIFVRYC

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CitM (TC 2.A.11) transporter family [H]

Homologues:

Organism=Homo sapiens, GI157266326, Length=185, Percent_Identity=32.4324324324324, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI87082277, Length=375, Percent_Identity=22.1333333333333, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574683, Length=451, Percent_Identity=26.6075388026608, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI28574681, Length=451, Percent_Identity=26.6075388026608, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI28574685, Length=451, Percent_Identity=26.6075388026608, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI28574678, Length=451, Percent_Identity=26.6075388026608, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19920704, Length=451, Percent_Identity=26.6075388026608, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24581759, Length=451, Percent_Identity=26.6075388026608, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI221472711, Length=210, Percent_Identity=32.8571428571429, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24581764, Length=210, Percent_Identity=32.8571428571429, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45550608, Length=438, Percent_Identity=24.8858447488584, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI85724998, Length=495, Percent_Identity=20.4040404040404, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI62484377, Length=495, Percent_Identity=20.4040404040404, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24656008, Length=414, Percent_Identity=26.5700483091787, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802
- InterPro:   IPR004680 [H]

Pfam domain/function: PF03600 CitMHS [H]

EC number: NA

Molecular weight: Translated: 48596; Mature: 48596

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEQVLALIIFISMFVLIVLDKIERHYVTLGCGALTLIGVFGIIMRSPEAVISTLNLRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IFTSGFWYQSGAAEEAVTGINWSTIIFIAGMMIMVEVMAAAGFFRWLCMLLAKAVHYRTR
HHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFITFMIMSFGLAMFIDSITVILFLAAVSIELAKLLKFNPVPMILSEIFCANLGGSATM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
CGDPPNIIIGTSLNFSFSTFITHTGLIAVISLVFIVVYFLIAYRKELESSPDIDKLAESM
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
PSPAETITNRRDFALSCVIFMCAVVMLVTHALTGLTVAFIGLVIAIFSLIVSGRKALHML
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSIDYKTILFFLGLFIVVGGLEQTGILEIIAAFIGRVSKGNLKLMVAIIIIISACASALI
HHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
DNIPFAATMVPVIKSLAATQGVDLSVLAWALSMGTDVGGSATPIGASANVVGTSVAAKNG
HCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCC
YMIGWGKYCRTAVPATIIVVLISMVCIFVRYC
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLAEQVLALIIFISMFVLIVLDKIERHYVTLGCGALTLIGVFGIIMRSPEAVISTLNLRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IFTSGFWYQSGAAEEAVTGINWSTIIFIAGMMIMVEVMAAAGFFRWLCMLLAKAVHYRTR
HHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFITFMIMSFGLAMFIDSITVILFLAAVSIELAKLLKFNPVPMILSEIFCANLGGSATM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
CGDPPNIIIGTSLNFSFSTFITHTGLIAVISLVFIVVYFLIAYRKELESSPDIDKLAESM
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
PSPAETITNRRDFALSCVIFMCAVVMLVTHALTGLTVAFIGLVIAIFSLIVSGRKALHML
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSIDYKTILFFLGLFIVVGGLEQTGILEIIAAFIGRVSKGNLKLMVAIIIIISACASALI
HHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
DNIPFAATMVPVIKSLAATQGVDLSVLAWALSMGTDVGGSATPIGASANVVGTSVAAKNG
HCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCC
YMIGWGKYCRTAVPATIIVVLISMVCIFVRYC
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]