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The map label for this gene is bioH

Identifier: 238921500

GI number: 238921500

Start: 3539263

End: 3540039

Strand: Reverse

Name: bioH

Synonym: NT01EI_3652

Alternate gene names: 238921500

Gene position: 3540039-3539263 (Counterclockwise)

Preceding gene: 238921503

Following gene: 238921496

Centisome position: 92.86

GC content: 65.64

Gene sequence:

>777_bases
ATGAGTGCGCTGTTTTGGCATACCTGCGGCGCGGGTAAACGCGATCTTGTGCTGCTGCACGGCTGGGGACTGAATGCTGA
AGTGTGGCGTAGCATTGTCCCCCAGCTCTCTGCGCAGTTTCGCCTGCACCTGGTCGATCTGCCCGGTTATGGGCGCAGCG
GCGGTGATACGCCGTATTCGCTGGCGGAGATGACACAGCGGGTGTTGGCGCAGGCACCGGAGCGGGCGCTATGGCTGGGG
TGGTCGCTGGGGGGGCTCGTTGCCACCCAGGCGGCCCTGTATCACCCGCAGCGGGTCAGCGGTTTAATCACCGTCGCCTC
TTCGCCCTGTTTTACGGCGCAGATGGCGTGGCCGGGGATCCGTAGTGATGTGCTGTACCATTTTCAATCACAGCTGCGCG
ATGATTTTCAGCGTACGGTGGAGCGTTTTCTGGCGCTGCAGACGCTGGGGGCCGACAGCGCCCGGCAGGATACCCGCGCG
CTGAAGTCGGTGGTGCTGGCGCAGCCGATGCCGTCGGCGGCGGTATTGAACGCGGGGTTGTCCCTGCTGCGCGAAACCGA
TCTGCGCCCGCAGCTGGCGACGCTGGCGCTGCCGTGGCTGCGCTTCTATGGCGCGCTGGATGGCCTGGTACCGCGCCGGG
TGGCGCCGCTGGTGGATGCGCTCAGTCCGTGCGGCCGTTCGCTGATTATTCCCGGTGCCGCCCATGCCCCCTTTATCTCT
CATCCCGAGCCGTTTTGCGCCGCGCTGTGCGAGTTTGCCGACGGGCGGTCGGTATAA

Upstream 100 bases:

>100_bases
ACTGGCGCCCGTAGGGGAAGGAGGCAGATCGCGCAGCGTCCGTGAAGTGATACCATAGGCTTCGTCCTGAAAGGTCTAAG
GGAAAAGGAGACCATAACCG

Downstream 100 bases:

>100_bases
TGGGCAGCGATAAAAAAAGCAGCCGCATCTGCGGCTGCCTGTCGAGGGGCGTCAATGGTTACGGCGCGCGTTTAGTGCAG
CGTGCGTATCCGGTAGACGA

Product: carboxylesterase BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSALFWHTCGAGKRDLVLLHGWGLNAEVWRSIVPQLSAQFRLHLVDLPGYGRSGGDTPYSLAEMTQRVLAQAPERALWLG
WSLGGLVATQAALYHPQRVSGLITVASSPCFTAQMAWPGIRSDVLYHFQSQLRDDFQRTVERFLALQTLGADSARQDTRA
LKSVVLAQPMPSAAVLNAGLSLLRETDLRPQLATLALPWLRFYGALDGLVPRRVAPLVDALSPCGRSLIIPGAAHAPFIS
HPEPFCAALCEFADGRSV

Sequences:

>Translated_258_residues
MSALFWHTCGAGKRDLVLLHGWGLNAEVWRSIVPQLSAQFRLHLVDLPGYGRSGGDTPYSLAEMTQRVLAQAPERALWLG
WSLGGLVATQAALYHPQRVSGLITVASSPCFTAQMAWPGIRSDVLYHFQSQLRDDFQRTVERFLALQTLGADSARQDTRA
LKSVVLAQPMPSAAVLNAGLSLLRETDLRPQLATLALPWLRFYGALDGLVPRRVAPLVDALSPCGRSLIIPGAAHAPFIS
HPEPFCAALCEFADGRSV
>Mature_257_residues
SALFWHTCGAGKRDLVLLHGWGLNAEVWRSIVPQLSAQFRLHLVDLPGYGRSGGDTPYSLAEMTQRVLAQAPERALWLGW
SLGGLVATQAALYHPQRVSGLITVASSPCFTAQMAWPGIRSDVLYHFQSQLRDDFQRTVERFLALQTLGADSARQDTRAL
KSVVLAQPMPSAAVLNAGLSLLRETDLRPQLATLALPWLRFYGALDGLVPRRVAPLVDALSPCGRSLIIPGAAHAPFISH
PEPFCAALCEFADGRSV

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=250, Percent_Identity=62.8, Blast_Score=321, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_EDWI9 (C5BGT3)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002935015.1
- ProteinModelPortal:   C5BGT3
- GeneID:   7962160
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3652
- OMA:   HWPQKER
- ProtClustDB:   CLSK2813701
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 28076; Mature: 27945

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: ACT_SITE 82-82 ACT_SITE 207-207 ACT_SITE 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALFWHTCGAGKRDLVLLHGWGLNAEVWRSIVPQLSAQFRLHLVDLPGYGRSGGDTPYS
CCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCHH
LAEMTQRVLAQAPERALWLGWSLGGLVATQAALYHPQRVSGLITVASSPCFTAQMAWPGI
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCHHHHCEEEEECCCCCEEEECCCCCH
RSDVLYHFQSQLRDDFQRTVERFLALQTLGADSARQDTRALKSVVLAQPMPSAAVLNAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
SLLRETDLRPQLATLALPWLRFYGALDGLVPRRVAPLVDALSPCGRSLIIPGAAHAPFIS
HHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
HPEPFCAALCEFADGRSV
CCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SALFWHTCGAGKRDLVLLHGWGLNAEVWRSIVPQLSAQFRLHLVDLPGYGRSGGDTPYS
CCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCHH
LAEMTQRVLAQAPERALWLGWSLGGLVATQAALYHPQRVSGLITVASSPCFTAQMAWPGI
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCHHHHCEEEEECCCCCEEEECCCCCH
RSDVLYHFQSQLRDDFQRTVERFLALQTLGADSARQDTRALKSVVLAQPMPSAAVLNAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
SLLRETDLRPQLATLALPWLRFYGALDGLVPRRVAPLVDALSPCGRSLIIPGAAHAPFIS
HHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
HPEPFCAALCEFADGRSV
CCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA