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The map label for this gene is tusB [H]

Identifier: 238921448

GI number: 238921448

Start: 3487943

End: 3488233

Strand: Reverse

Name: tusB [H]

Synonym: NT01EI_3600

Alternate gene names: 238921448

Gene position: 3488233-3487943 (Counterclockwise)

Preceding gene: 238921449

Following gene: 238921447

Centisome position: 91.5

GC content: 62.2

Gene sequence:

>291_bases
ATGTTACATACCTTGACCCGATCAGCGCAGGGGGGCATCGATTTCACTGCGCTGGCGGCGTTGGTGCTCCCCGGTGATGC
GCTGGTTCTGTTGCAGGATGGTGTACTGTGCGCGCTGATGGGCAGCGCCGGGCTGGCGGCACTGAACGCTATGCCGGTGA
CTCTGCATGTCTTGCGGGAGGATGTGCTGGCGCGGGGGCTGACAGCGTATGTCGCACCGGCGTGTCAGCTGATTGGCTAT
GGCGAATTTGTCGCGTTGAGCGAGGCGCATTCGCATCAGATGGCCTGGTGA

Upstream 100 bases:

>100_bases
GCTGGGTTATTCCTGCCGATGTGCTGGCGGCCGATGCGTTGCGCGCGCGTCTGGATGCGTGCGACGTCATTTTGACCTTT
TAATACCGGGGGGTTCTGCG

Downstream 100 bases:

>100_bases
CGGTACAGCCAGTGTTGACAGTATTGCGCCGAATATCTTGTATATTTCTTGACACCCTCCCGACTCAGCCATAAAATTCT
GCGTCCCCATATTTTGCCCT

Product: protein TusB

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein B [H]

Number of amino acids: Translated: 96; Mature: 96

Protein sequence:

>96_residues
MLHTLTRSAQGGIDFTALAALVLPGDALVLLQDGVLCALMGSAGLAALNAMPVTLHVLREDVLARGLTAYVAPACQLIGY
GEFVALSEAHSHQMAW

Sequences:

>Translated_96_residues
MLHTLTRSAQGGIDFTALAALVLPGDALVLLQDGVLCALMGSAGLAALNAMPVTLHVLREDVLARGLTAYVAPACQLIGY
GEFVALSEAHSHQMAW
>Mature_96_residues
MLHTLTRSAQGGIDFTALAALVLPGDALVLLQDGVLCALMGSAGLAALNAMPVTLHVLREDVLARGLTAYVAPACQLIGY
GEFVALSEAHSHQMAW

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions [H]

COG id: COG2168

COG function: function code P; Uncharacterized conserved protein involved in oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrH/tusB family [H]

Homologues:

Organism=Escherichia coli, GI1789741, Length=96, Percent_Identity=46.875, Blast_Score=81, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007215 [H]

Pfam domain/function: PF04077 DsrH [H]

EC number: NA

Molecular weight: Translated: 9971; Mature: 9971

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTLTRSAQGGIDFTALAALVLPGDALVLLQDGVLCALMGSAGLAALNAMPVTLHVLRE
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCEEEEECCCCCHHHHHCCHHHHHHHHH
DVLARGLTAYVAPACQLIGYGEFVALSEAHSHQMAW
HHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MLHTLTRSAQGGIDFTALAALVLPGDALVLLQDGVLCALMGSAGLAALNAMPVTLHVLRE
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCEEEEECCCCCHHHHHCCHHHHHHHHH
DVLARGLTAYVAPACQLIGYGEFVALSEAHSHQMAW
HHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA