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The map label for this gene is guaD [H]

Identifier: 238921375

GI number: 238921375

Start: 3412518

End: 3413834

Strand: Reverse

Name: guaD [H]

Synonym: NT01EI_3519

Alternate gene names: 238921375

Gene position: 3413834-3412518 (Counterclockwise)

Preceding gene: 238921376

Following gene: 238921374

Centisome position: 89.55

GC content: 58.01

Gene sequence:

>1317_bases
ATGGTTTGTGAACATACCCTCAAGGCAGTCCGCGGTAGCATTCTGGATGTCACCCGTACGGTGACCGATCCGCAACAGGT
TGAATCTGCACTGCGTTTTATTGAGGACGGTCTGTTATTGATCCGCAATGGGAAAGTGGACTGGTGCGGCAGCTGGGAAG
AGGGGCGGGGGCGCATTCCCGCATCGATTCGGGTGCGGGACTACCGCGGCAAGCTGATCGTCCCCGGCTTTGTCGATACC
CATATTCACTATCCGCAGAGCGAGATGGTCGGGGCCTATGGCGAACAGCTGTTGGAGTGGTTGAACAAGCACACCTTTCC
CACCGAGCAGCGCTATAACGATCTCGATTACGCCCGCGAAATGTCTAACTTTTTCCTTAAACAGCTGCTGCGTAACGGCA
CCACCACCGCCCTGGTGTTCGGCACCGTGCATCCGGAGTCGGTGGATGCGCTGTTTGAGGCTGCCAGCCATATCAACATG
CGGATGATCGCCGGCAAGGTAATGATGGATCGCAATGCGCCGGACTATCTGTTGGACGATGCGCAGAGCAGCTACGAACA
GAGCAAGGCACTGATCGAGCGCTGGCACAAGAATGGCCGCCTGCTGTATGCGGTGACCCCGCGCTTTGCACCGACCTCCA
GTCCGGAGCAGCTGGCGATGGCCCAGCGCCTGCGCGAAGAGTATCCCGACACCTATTTGCATACCCATCTGTGTGAAAAC
CGCGACGAGATCGCCTGGGTCAAGTCGCTGTACCCGCACCATGACGGCTATCTGGATGTGTATCACCAGTATGGCCTGAC
CGGGAAGAACTGCGTGTTCGCCCACTGTATCCACCTGGAGGAGAAAGAGTGGGACTGCCTGAGCGATACCGGTTCTTCGA
TCGCCTTCTGCCCGACCTCTAACCTGTACCTCGGCAGCGGCCTGTTTAACCTGCAACAGGCCTGGCGCAAGCGGGTGAAG
GTGGGGATGGGAACCGACATCGGCGCCGGGACCACCTTTAACATGCTGCAGACGCTGAATGAGGCTTACAAGGTGATGCA
GCTGCAGGGCTGCCGCTTGTCCGCCTATGAGGCGTTCTACCTGGCAACCCTGGGCGGCGCGGCCTCTCTGGGGCTGGACG
ATCGCATCGGTAACTTCCTGGTCGGTAAAGAGGCCGACTTTGTGGTTCTGGAGCCGACGGCGACGCCGCTGCAACAGCTG
CGTTATGACAACTCGGTCTCTTTGGTGGATAAGCTGTTTGTCACCATGACACTGGGCGATGATCGCACCATATATCGTAC
CTATGTCGATGGTCGTTTAGTGTACGAACGCACCTAA

Upstream 100 bases:

>100_bases
GAAAGCGGAGGTCCGTCGGGAAGAGGATGAACCGACAGGCACATTGTCTGCAGAAGAAGAGATGGACTGAGTCGATGTCT
TGGCTGATTAGGAGTTAATC

Downstream 100 bases:

>100_bases
AATGTCGCGTTTGTGGGTTGAAATGACGAGGTATTAGGCCAGATAATCGTTTGCCTTTTGCTTCTGTCCCATTTTTACCC
GAATTTGGTTACAGGATGTA

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVCEHTLKAVRGSILDVTRTVTDPQQVESALRFIEDGLLLIRNGKVDWCGSWEEGRGRIPASIRVRDYRGKLIVPGFVDT
HIHYPQSEMVGAYGEQLLEWLNKHTFPTEQRYNDLDYAREMSNFFLKQLLRNGTTTALVFGTVHPESVDALFEAASHINM
RMIAGKVMMDRNAPDYLLDDAQSSYEQSKALIERWHKNGRLLYAVTPRFAPTSSPEQLAMAQRLREEYPDTYLHTHLCEN
RDEIAWVKSLYPHHDGYLDVYHQYGLTGKNCVFAHCIHLEEKEWDCLSDTGSSIAFCPTSNLYLGSGLFNLQQAWRKRVK
VGMGTDIGAGTTFNMLQTLNEAYKVMQLQGCRLSAYEAFYLATLGGAASLGLDDRIGNFLVGKEADFVVLEPTATPLQQL
RYDNSVSLVDKLFVTMTLGDDRTIYRTYVDGRLVYERT

Sequences:

>Translated_438_residues
MVCEHTLKAVRGSILDVTRTVTDPQQVESALRFIEDGLLLIRNGKVDWCGSWEEGRGRIPASIRVRDYRGKLIVPGFVDT
HIHYPQSEMVGAYGEQLLEWLNKHTFPTEQRYNDLDYAREMSNFFLKQLLRNGTTTALVFGTVHPESVDALFEAASHINM
RMIAGKVMMDRNAPDYLLDDAQSSYEQSKALIERWHKNGRLLYAVTPRFAPTSSPEQLAMAQRLREEYPDTYLHTHLCEN
RDEIAWVKSLYPHHDGYLDVYHQYGLTGKNCVFAHCIHLEEKEWDCLSDTGSSIAFCPTSNLYLGSGLFNLQQAWRKRVK
VGMGTDIGAGTTFNMLQTLNEAYKVMQLQGCRLSAYEAFYLATLGGAASLGLDDRIGNFLVGKEADFVVLEPTATPLQQL
RYDNSVSLVDKLFVTMTLGDDRTIYRTYVDGRLVYERT
>Mature_438_residues
MVCEHTLKAVRGSILDVTRTVTDPQQVESALRFIEDGLLLIRNGKVDWCGSWEEGRGRIPASIRVRDYRGKLIVPGFVDT
HIHYPQSEMVGAYGEQLLEWLNKHTFPTEQRYNDLDYAREMSNFFLKQLLRNGTTTALVFGTVHPESVDALFEAASHINM
RMIAGKVMMDRNAPDYLLDDAQSSYEQSKALIERWHKNGRLLYAVTPRFAPTSSPEQLAMAQRLREEYPDTYLHTHLCEN
RDEIAWVKSLYPHHDGYLDVYHQYGLTGKNCVFAHCIHLEEKEWDCLSDTGSSIAFCPTSNLYLGSGLFNLQQAWRKRVK
VGMGTDIGAGTTFNMLQTLNEAYKVMQLQGCRLSAYEAFYLATLGGAASLGLDDRIGNFLVGKEADFVVLEPTATPLQQL
RYDNSVSLVDKLFVTMTLGDDRTIYRTYVDGRLVYERT

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=405, Percent_Identity=36.2962962962963, Blast_Score=237, Evalue=1e-62,
Organism=Escherichia coli, GI1789249, Length=437, Percent_Identity=87.4141876430206, Blast_Score=816, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6319963, Length=418, Percent_Identity=33.0143540669856, Blast_Score=222, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24643849, Length=446, Percent_Identity=31.6143497757848, Blast_Score=207, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 49801; Mature: 49801

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCEHTLKAVRGSILDVTRTVTDPQQVESALRFIEDGLLLIRNGKVDWCGSWEEGRGRIP
CCCHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCC
ASIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTEQRYNDLDYARE
CEEEEEECCCCEEECCEECCCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHH
MSNFFLKQLLRNGTTTALVFGTVHPESVDALFEAASHINMRMIAGKVMMDRNAPDYLLDD
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCEECCCCCCHHHHH
AQSSYEQSKALIERWHKNGRLLYAVTPRFAPTSSPEQLAMAQRLREEYPDTYLHTHLCEN
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCC
RDEIAWVKSLYPHHDGYLDVYHQYGLTGKNCVFAHCIHLEEKEWDCLSDTGSSIAFCPTS
CHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHCCCCEEEECCCC
NLYLGSGLFNLQQAWRKRVKVGMGTDIGAGTTFNMLQTLNEAYKVMQLQGCRLSAYEAFY
CEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LATLGGAASLGLDDRIGNFLVGKEADFVVLEPTATPLQQLRYDNSVSLVDKLFVTMTLGD
EEECCCCCCCCCHHHCCCEEECCCCCEEEECCCCCHHHHHCCCCCHHHHHEEEEEEEECC
DRTIYRTYVDGRLVYERT
CCEEEEEEECCEEEEECC
>Mature Secondary Structure
MVCEHTLKAVRGSILDVTRTVTDPQQVESALRFIEDGLLLIRNGKVDWCGSWEEGRGRIP
CCCHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCC
ASIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTEQRYNDLDYARE
CEEEEEECCCCEEECCEECCCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHH
MSNFFLKQLLRNGTTTALVFGTVHPESVDALFEAASHINMRMIAGKVMMDRNAPDYLLDD
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCEECCCCCCHHHHH
AQSSYEQSKALIERWHKNGRLLYAVTPRFAPTSSPEQLAMAQRLREEYPDTYLHTHLCEN
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCC
RDEIAWVKSLYPHHDGYLDVYHQYGLTGKNCVFAHCIHLEEKEWDCLSDTGSSIAFCPTS
CHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHCCCCEEEECCCC
NLYLGSGLFNLQQAWRKRVKVGMGTDIGAGTTFNMLQTLNEAYKVMQLQGCRLSAYEAFY
CEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LATLGGAASLGLDDRIGNFLVGKEADFVVLEPTATPLQQLRYDNSVSLVDKLFVTMTLGD
EEECCCCCCCCCHHHCCCEEECCCCCEEEECCCCCHHHHHCCCCCHHHHHEEEEEEEECC
DRTIYRTYVDGRLVYERT
CCEEEEEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]