Click here to switch to the map view.

The map label for this gene is yafV [C]

Identifier: 238921341

GI number: 238921341

Start: 3380034

End: 3380876

Strand: Reverse

Name: yafV [C]

Synonym: NT01EI_3485

Alternate gene names: 238921341

Gene position: 3380876-3380034 (Counterclockwise)

Preceding gene: 238921342

Following gene: 238921340

Centisome position: 88.68

GC content: 62.75

Gene sequence:

>843_bases
ATGAGGAATGCCAACGTCGCCTTACTGCAACTGTGCAGCGGCGAAAACAGCCGCGCCAACCTGGCACAGATTGAGCAGCA
GTTGAAGCAACTCAACGGCAATATCAGGCTGGCGTTGACCCCGGAGAATGCGCTGCTGTTCAGCGACGCCGCGGGGTACC
GCCAGCACGCTGAGCGGGAGGGTGACGGTCCGCTTCAGGCGGCGGTGTGCTCTCTGGCACGTCGCTACGGGGTGTGGCTG
TTGGTCGGCTCAATGCCGCTGTACAGCCGCGAGGATGTCGGTGGCATTACCTCCAGCAGTCTGCTGTTTGACGACGGCGG
CACGCTGCGCGCCCGCTACGATAAGCTGCACATGTTCGACGTCGAGGTGCGTGACGCGCACGGCCGTTACCGCGAGTCTG
ACGTTTACCGTCACGGTGAAATGGTGACGGTGGTGGATACGCCGGTTGGCCGCCTGGGGATGACCATTTGCTACGATCTG
CGCTTTCCCGGCCTGTTCCAGGCGCTGCGCGCGCAGGGGGCGGAGATTATCTCGGTACCGGCTGCTTTTACCCGGGTGAC
GGGGGAGGCGCATTGGGAGGTGCTGCTGCGCGCCCGGGCGATAGAAAACCAGTGCTATATTCTGGCGTCGGCCCAGGTCG
GCAGCCACGGAGATACCCGGCGCACCTGGGGCCACAGCATGGCAGTGGACGGCTGGGGCCGCGTGCTGCAGCAGAACACC
GACGCTGTACGGCCGATAAAGATTAACGTGAATACCAACTCACTGAGCGTGCTGCGCGATCAGATGCCACTGGCACAGCA
CAACCGATTCCAGCCTGAGCTGATCCATCTTCACCAAGGGTAA

Upstream 100 bases:

>100_bases
TGTGGAATAAAATATCGCTGATACGCTACAACATAAGCGGTGGGCTCGACAGCCCGACGATCCATGAAGTGTTACGTCAG
CCGAAGAAGGAGAAGGCACA

Downstream 100 bases:

>100_bases
ATACACCATGAGTCTGACGTTTGTCAGTGAGCAGTTACTGGCTGCCAACAATCTGAATCATCAGGATTTATATAATGTCT
TGGGGCAGTTGAATGCACGG

Product: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRNANVALLQLCSGENSRANLAQIEQQLKQLNGNIRLALTPENALLFSDAAGYRQHAEREGDGPLQAAVCSLARRYGVWL
LVGSMPLYSREDVGGITSSSLLFDDGGTLRARYDKLHMFDVEVRDAHGRYRESDVYRHGEMVTVVDTPVGRLGMTICYDL
RFPGLFQALRAQGAEIISVPAAFTRVTGEAHWEVLLRARAIENQCYILASAQVGSHGDTRRTWGHSMAVDGWGRVLQQNT
DAVRPIKINVNTNSLSVLRDQMPLAQHNRFQPELIHLHQG

Sequences:

>Translated_280_residues
MRNANVALLQLCSGENSRANLAQIEQQLKQLNGNIRLALTPENALLFSDAAGYRQHAEREGDGPLQAAVCSLARRYGVWL
LVGSMPLYSREDVGGITSSSLLFDDGGTLRARYDKLHMFDVEVRDAHGRYRESDVYRHGEMVTVVDTPVGRLGMTICYDL
RFPGLFQALRAQGAEIISVPAAFTRVTGEAHWEVLLRARAIENQCYILASAQVGSHGDTRRTWGHSMAVDGWGRVLQQNT
DAVRPIKINVNTNSLSVLRDQMPLAQHNRFQPELIHLHQG
>Mature_280_residues
MRNANVALLQLCSGENSRANLAQIEQQLKQLNGNIRLALTPENALLFSDAAGYRQHAEREGDGPLQAAVCSLARRYGVWL
LVGSMPLYSREDVGGITSSSLLFDDGGTLRARYDKLHMFDVEVRDAHGRYRESDVYRHGEMVTVVDTPVGRLGMTICYDL
RFPGLFQALRAQGAEIISVPAAFTRVTGEAHWEVLLRARAIENQCYILASAQVGSHGDTRRTWGHSMAVDGWGRVLQQNT
DAVRPIKINVNTNSLSVLRDQMPLAQHNRFQPELIHLHQG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=272, Percent_Identity=36.3970588235294, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI297632350, Length=272, Percent_Identity=36.3970588235294, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI297632348, Length=272, Percent_Identity=36.3970588235294, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI9910460, Length=198, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI297632346, Length=196, Percent_Identity=32.6530612244898, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1786412, Length=250, Percent_Identity=26.8, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=209, Percent_Identity=43.0622009569378, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6322335, Length=298, Percent_Identity=31.2080536912752, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323383, Length=278, Percent_Identity=30.2158273381295, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17933642, Length=271, Percent_Identity=37.2693726937269, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI21355835, Length=269, Percent_Identity=28.6245353159851, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 31159; Mature: 31159

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNANVALLQLCSGENSRANLAQIEQQLKQLNGNIRLALTPENALLFSDAAGYRQHAERE
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHCCC
GDGPLQAAVCSLARRYGVWLLVGSMPLYSREDVGGITSSSLLFDDGGTLRARYDKLHMFD
CCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEEEEECEEEEEE
VEVRDAHGRYRESDVYRHGEMVTVVDTPVGRLGMTICYDLRFPGLFQALRAQGAEIISVP
EEEECCCCCCCCCHHHHCCCEEEEECCCCCHHCEEEEEECCCCHHHHHHHHCCCEEEECC
AAFTRVTGEAHWEVLLRARAIENQCYILASAQVGSHGDTRRTWGHSMAVDGWGRVLQQNT
HHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHCCCCEEEHHHHHHHHCCC
DAVRPIKINVNTNSLSVLRDQMPLAQHNRFQPELIHLHQG
CCCCEEEEEECCCHHHHHHHCCCHHHCCCCCCEEEEEECC
>Mature Secondary Structure
MRNANVALLQLCSGENSRANLAQIEQQLKQLNGNIRLALTPENALLFSDAAGYRQHAERE
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHCCC
GDGPLQAAVCSLARRYGVWLLVGSMPLYSREDVGGITSSSLLFDDGGTLRARYDKLHMFD
CCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEEEEECEEEEEE
VEVRDAHGRYRESDVYRHGEMVTVVDTPVGRLGMTICYDLRFPGLFQALRAQGAEIISVP
EEEECCCCCCCCCHHHHCCCEEEEECCCCCHHCEEEEEECCCCHHHHHHHHCCCEEEECC
AAFTRVTGEAHWEVLLRARAIENQCYILASAQVGSHGDTRRTWGHSMAVDGWGRVLQQNT
HHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHCCCCEEEHHHHHHHHCCC
DAVRPIKINVNTNSLSVLRDQMPLAQHNRFQPELIHLHQG
CCCCEEEEEECCCHHHHHHHCCCHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]