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The map label for this gene is melA [H]

Identifier: 238921279

GI number: 238921279

Start: 3307405

End: 3308784

Strand: Reverse

Name: melA [H]

Synonym: NT01EI_3422

Alternate gene names: 238921279

Gene position: 3308784-3307405 (Counterclockwise)

Preceding gene: 238921280

Following gene: 238921276

Centisome position: 86.79

GC content: 64.93

Gene sequence:

>1380_bases
ATGAGCAAGATCACCCTGATCGGCGCGGGCAGTATTATGTTTTCGCGTCAGATCATTTCCGCCCTGCTGTCCAGCCCGGT
GCTGGCGGGCAGCGAAATCATCCTGATGGATCTGGATGAACAGGTGCTGGCGCGCAGCCAGCGCCTGATCGGTATGATGG
TGGCACAGTCGGGGGTGCCGGTGACGGTACGCCACACCACCGACAGACGCAGCGCGCTGCGCGGCGCCGACTTTGTCATC
AACGCCATTCAGGTGGGCGGGCTGGCACCCTGGCGGCTGGACATGGAGATTCCGGCGCGCTACGGCGTGATTCAGGAGGT
GGGTGACACCCTGGGGCCCGGCGGTATCTTCCGCGCCCTGCGCCATATTCCCCCGATGCTGGATATTCTGCGCGATATGG
AGGATCTGTGCCCGCAGGCGCTGTTCATCAACTATGCCAATCCGCTGGCGCCGTTGACCTGGGCGGCCAAAGAGGCCAGC
GGCGTGCGCAGCATCGGCCTGTGCTATGGAGTGCGCTATACCGTGGCGCAGCTGGTCGGCTATCTGGGGCTGGGGCCGTG
GGTTGACCACCCCTCCACACCGCAGCGCTGGCAGCGCCTGATGTATCACGAGGTCCCGGCCGATATCGCGTATGAGTTTG
CCGGGATCAACCACATGACCTGGATTACGGCGCTGTCACGCCATGGTGAGGATCTGCTGCCGCGGGTGCGGGCACTGGCC
GATGACGCGCGTACCCGCCAGGCTGACGGGGTACGTTGCGAGGTGCTGCGCTTCTTCGGCCTGTGGTGTACCGAGAACCA
CTGGCACTGCAGCGACTATCTGCCCTACTTCCGCAAGAATCCGCAGATGATCGATCGCTTCCTGCCGCAGCGCTGGAACC
TGCTGGCGCTGGAGGAGCAGGTGCATGCCGCCGGCCAGGCGGAGATCGATGCCCAGCTGGCCGGCGAGCGTCCTTTCGTT
ATCGCGCCGAACATGCTCAACGCGCCCAAGCTGATCAGTGCACAGCTCAGCGGTGAGCGCATCCGTATCAACGGCAATAT
GGCCAACCGTCAGCCGCAGGGGCTGCTGGTGGAGAATCTACCGGCGGAGTGCGTGGTGGAGGTGCCGGTTTGGGTCGACG
GTGACGGGCTGCACCCCCAGACGATGGGGCGTCTGCCGACCCAGTGCGCCAGCCTGTGTAAGAGCAATATCGCGGTGCAG
GAGCTGGTGGTGCAGGCGGCGCTGACCGGCGATCTGGACGCGGCGCGCTATGCGCTCTCTCTCGATCCGCTGACCGCCGC
CGTCTGTACCCTGGATCAGATCCAGTCGATGTTCGACGCGCTGTTTGCCGCCCAGCGGCAGTGGCTGCCGCAGTTTGACG
CCGCACAGGCGTCAGCCTGA

Upstream 100 bases:

>100_bases
ATTCTGGCGGCGCTGGTGGTGCATGGCTCTCTGCTGCAGCGCCGCCCGCGACGGCGTTAAGGCTGCGCCGTCGGCGCAGC
GTATTGATTAAGGCAATGTA

Downstream 100 bases:

>100_bases
GGCGTAAGGGGGGCACTCCGCCCCCTTACGGAGATCTCTTGCGGCTACATGAGGCCGTATTGCCTGCCAAGGTGGTGGTG
TGATCAGTGATGTGATGCGG

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSKITLIGAGSIMFSRQIISALLSSPVLAGSEIILMDLDEQVLARSQRLIGMMVAQSGVPVTVRHTTDRRSALRGADFVI
NAIQVGGLAPWRLDMEIPARYGVIQEVGDTLGPGGIFRALRHIPPMLDILRDMEDLCPQALFINYANPLAPLTWAAKEAS
GVRSIGLCYGVRYTVAQLVGYLGLGPWVDHPSTPQRWQRLMYHEVPADIAYEFAGINHMTWITALSRHGEDLLPRVRALA
DDARTRQADGVRCEVLRFFGLWCTENHWHCSDYLPYFRKNPQMIDRFLPQRWNLLALEEQVHAAGQAEIDAQLAGERPFV
IAPNMLNAPKLISAQLSGERIRINGNMANRQPQGLLVENLPAECVVEVPVWVDGDGLHPQTMGRLPTQCASLCKSNIAVQ
ELVVQAALTGDLDAARYALSLDPLTAAVCTLDQIQSMFDALFAAQRQWLPQFDAAQASA

Sequences:

>Translated_459_residues
MSKITLIGAGSIMFSRQIISALLSSPVLAGSEIILMDLDEQVLARSQRLIGMMVAQSGVPVTVRHTTDRRSALRGADFVI
NAIQVGGLAPWRLDMEIPARYGVIQEVGDTLGPGGIFRALRHIPPMLDILRDMEDLCPQALFINYANPLAPLTWAAKEAS
GVRSIGLCYGVRYTVAQLVGYLGLGPWVDHPSTPQRWQRLMYHEVPADIAYEFAGINHMTWITALSRHGEDLLPRVRALA
DDARTRQADGVRCEVLRFFGLWCTENHWHCSDYLPYFRKNPQMIDRFLPQRWNLLALEEQVHAAGQAEIDAQLAGERPFV
IAPNMLNAPKLISAQLSGERIRINGNMANRQPQGLLVENLPAECVVEVPVWVDGDGLHPQTMGRLPTQCASLCKSNIAVQ
ELVVQAALTGDLDAARYALSLDPLTAAVCTLDQIQSMFDALFAAQRQWLPQFDAAQASA
>Mature_458_residues
SKITLIGAGSIMFSRQIISALLSSPVLAGSEIILMDLDEQVLARSQRLIGMMVAQSGVPVTVRHTTDRRSALRGADFVIN
AIQVGGLAPWRLDMEIPARYGVIQEVGDTLGPGGIFRALRHIPPMLDILRDMEDLCPQALFINYANPLAPLTWAAKEASG
VRSIGLCYGVRYTVAQLVGYLGLGPWVDHPSTPQRWQRLMYHEVPADIAYEFAGINHMTWITALSRHGEDLLPRVRALAD
DARTRQADGVRCEVLRFFGLWCTENHWHCSDYLPYFRKNPQMIDRFLPQRWNLLALEEQVHAAGQAEIDAQLAGERPFVI
APNMLNAPKLISAQLSGERIRINGNMANRQPQGLLVENLPAECVVEVPVWVDGDGLHPQTMGRLPTQCASLCKSNIAVQE
LVVQAALTGDLDAARYALSLDPLTAAVCTLDQIQSMFDALFAAQRQWLPQFDAAQASA

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=472, Percent_Identity=33.8983050847458, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788029, Length=482, Percent_Identity=25.9336099585062, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 50675; Mature: 50544

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITLIGAGSIMFSRQIISALLSSPVLAGSEIILMDLDEQVLARSQRLIGMMVAQSGVP
CCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCC
VTVRHTTDRRSALRGADFVINAIQVGGLAPWRLDMEIPARYGVIQEVGDTLGPGGIFRAL
EEEEECCHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHH
RHIPPMLDILRDMEDLCPQALFINYANPLAPLTWAAKEASGVRSIGLCYGVRYTVAQLVG
HHCCHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YLGLGPWVDHPSTPQRWQRLMYHEVPADIAYEFAGINHMTWITALSRHGEDLLPRVRALA
HHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
DDARTRQADGVRCEVLRFFGLWCTENHWHCSDYLPYFRKNPQMIDRFLPQRWNLLALEEQ
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHCCHHHHHHHCCCCCCEEEEHHH
VHAAGQAEIDAQLAGERPFVIAPNMLNAPKLISAQLSGERIRINGNMANRQPQGLLVENL
HHHCCCCCCHHHHCCCCCEEECCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCC
PAECVVEVPVWVDGDGLHPQTMGRLPTQCASLCKSNIAVQELVVQAALTGDLDAARYALS
CCCEEEEECEEECCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHEEE
LDPLTAAVCTLDQIQSMFDALFAAQRQWLPQFDAAQASA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SKITLIGAGSIMFSRQIISALLSSPVLAGSEIILMDLDEQVLARSQRLIGMMVAQSGVP
CEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCC
VTVRHTTDRRSALRGADFVINAIQVGGLAPWRLDMEIPARYGVIQEVGDTLGPGGIFRAL
EEEEECCHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHH
RHIPPMLDILRDMEDLCPQALFINYANPLAPLTWAAKEASGVRSIGLCYGVRYTVAQLVG
HHCCHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YLGLGPWVDHPSTPQRWQRLMYHEVPADIAYEFAGINHMTWITALSRHGEDLLPRVRALA
HHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
DDARTRQADGVRCEVLRFFGLWCTENHWHCSDYLPYFRKNPQMIDRFLPQRWNLLALEEQ
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHCCHHHHHHHCCCCCCEEEEHHH
VHAAGQAEIDAQLAGERPFVIAPNMLNAPKLISAQLSGERIRINGNMANRQPQGLLVENL
HHHCCCCCCHHHHCCCCCEEECCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCC
PAECVVEVPVWVDGDGLHPQTMGRLPTQCASLCKSNIAVQELVVQAALTGDLDAARYALS
CCCEEEEECEEECCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHEEE
LDPLTAAVCTLDQIQSMFDALFAAQRQWLPQFDAAQASA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]