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The map label for this gene is ygfZ [C]

Identifier: 238921204

GI number: 238921204

Start: 3229956

End: 3230951

Strand: Direct

Name: ygfZ [C]

Synonym: NT01EI_3346

Alternate gene names: 238921204

Gene position: 3229956-3230951 (Clockwise)

Preceding gene: 238921201

Following gene: 238921207

Centisome position: 84.72

GC content: 65.36

Gene sequence:

>996_bases
ATGAACCTTAATGCCCCCTTCTCCCCGCAGCCACCTCTGGCCGCAGAGCACCTTCCCCTGACCCTGATGCGGTTGGATGA
CTGGCTGCCGATTAACGTCAGTGGCCCGGATGCGCAAAGCTACCTGCAGGGGCAGCTTACCGCCGACCTGCCATCGCTGG
CCGCCACGCAGCATACGCTGTGCGGCCACTGCGATGCCCAAGGCAAGCTGTGGAGCAGCCTGCGTCTGCTGCGTCGCCGT
GATGGCTTCACCTACCTGCTGCGCCGTAGCGTAGCCACCCTGCAGATGCTGGAGCTGAAAAAGTATGCGGTATTTGCCAA
GGCCAGCATCGTCAGCGACGAGGGGGCGGTGCTGCTGGGGGTCGCCGGTGCACAGGCAAGCGAAGCCCTCGGCGCACTCT
TTCCACGGCTGCCGGACGCCGACGCGCCGCTGCTGCAGGCTGGCCGTAGCCATCTGCTCTATATGGCCTGGCCGCAGCCG
CGCTACCTGCTGATCTGCGATGATGCGGATGAAGCAGAACGGATCTTCGCCCCGCTGTCCGCACGTGCACGTCTGGCGGA
CAGCGCCCAGTGGCTGGCGCTGGATATTGAATCCGGGATCCCACTCATCGATGAGCCCAACTGCGATAGCTTCCTGCCCC
AAGCTGTAAACCTGCAAGCGCTGGGCGGGATCTCGTTTACCAAGGGATGCTACAGCGGTCAGGAGATGGTGGCGCGCGCC
AAATACCGCGGCGCCAACCGACGGGCGCTGTTCTGGCTGCGCGGTAGCGCAGAGCGACTGCCGCACGCCAGCGAAGATCT
GGAGCTGCGTCTGGGCGACAGCTGGCGACGCAGCGGCACGGTACTGGCGGCACAGCGGCTGGCTGACGGAGGCGTCTATC
TGCAGGCGGTGCTGAGCAGCGATCTCCCGGCTGACAGCGTACTGCGGGTACGCGACGACGCGCGGAGCCAGCTGACACTG
TCGCCCCTGCCCTATAGCACCGATCAGCAGGAGTGA

Upstream 100 bases:

>100_bases
TTCTGAACGTGGCCGCATCGAGCGCAATTAACCATTGGCATTGTAGGCGACCTTTCTTACCATGGTCACTACGTCCGGTA
TGACCTTACAGGGAACTCTC

Downstream 100 bases:

>100_bases
TTCCCGCCACGCCCGCCACAGCGACGTCCGTGGTCGGGCGTCACTCGCCCCGGGCGTGGGCCGCGGCCTGCAAAAACAGT
CCATGCTCCGGGCGAATGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNLNAPFSPQPPLAAEHLPLTLMRLDDWLPINVSGPDAQSYLQGQLTADLPSLAATQHTLCGHCDAQGKLWSSLRLLRRR
DGFTYLLRRSVATLQMLELKKYAVFAKASIVSDEGAVLLGVAGAQASEALGALFPRLPDADAPLLQAGRSHLLYMAWPQP
RYLLICDDADEAERIFAPLSARARLADSAQWLALDIESGIPLIDEPNCDSFLPQAVNLQALGGISFTKGCYSGQEMVARA
KYRGANRRALFWLRGSAERLPHASEDLELRLGDSWRRSGTVLAAQRLADGGVYLQAVLSSDLPADSVLRVRDDARSQLTL
SPLPYSTDQQE

Sequences:

>Translated_331_residues
MNLNAPFSPQPPLAAEHLPLTLMRLDDWLPINVSGPDAQSYLQGQLTADLPSLAATQHTLCGHCDAQGKLWSSLRLLRRR
DGFTYLLRRSVATLQMLELKKYAVFAKASIVSDEGAVLLGVAGAQASEALGALFPRLPDADAPLLQAGRSHLLYMAWPQP
RYLLICDDADEAERIFAPLSARARLADSAQWLALDIESGIPLIDEPNCDSFLPQAVNLQALGGISFTKGCYSGQEMVARA
KYRGANRRALFWLRGSAERLPHASEDLELRLGDSWRRSGTVLAAQRLADGGVYLQAVLSSDLPADSVLRVRDDARSQLTL
SPLPYSTDQQE
>Mature_331_residues
MNLNAPFSPQPPLAAEHLPLTLMRLDDWLPINVSGPDAQSYLQGQLTADLPSLAATQHTLCGHCDAQGKLWSSLRLLRRR
DGFTYLLRRSVATLQMLELKKYAVFAKASIVSDEGAVLLGVAGAQASEALGALFPRLPDADAPLLQAGRSHLLYMAWPQP
RYLLICDDADEAERIFAPLSARARLADSAQWLALDIESGIPLIDEPNCDSFLPQAVNLQALGGISFTKGCYSGQEMVARA
KYRGANRRALFWLRGSAERLPHASEDLELRLGDSWRRSGTVLAAQRLADGGVYLQAVLSSDLPADSVLRVRDDARSQLTL
SPLPYSTDQQE

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family

Homologues:

Organism=Escherichia coli, GI1789265, Length=324, Percent_Identity=54.6296296296296, Blast_Score=355, Evalue=2e-99,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YGFZ_EDWI9 (C5BAS5)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934719.1
- GeneID:   7959646
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3346
- OMA:   GAHCDPK
- ProtClustDB:   CLSK2813613
- GO:   GO:0005737
- HAMAP:   MF_01175
- InterPro:   IPR017703
- InterPro:   IPR006222
- TIGRFAMs:   TIGR03317

Pfam domain/function: PF01571 GCV_T

EC number: NA

Molecular weight: Translated: 36174; Mature: 36174

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNAPFSPQPPLAAEHLPLTLMRLDDWLPINVSGPDAQSYLQGQLTADLPSLAATQHTL
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHCCHHCCCCHHHHHHHHH
CGHCDAQGKLWSSLRLLRRRDGFTYLLRRSVATLQMLELKKYAVFAKASIVSDEGAVLLG
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEE
VAGAQASEALGALFPRLPDADAPLLQAGRSHLLYMAWPQPRYLLICDDADEAERIFAPLS
ECCCCHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECCCCCEEEEECCCCCHHHHHHCHH
ARARLADSAQWLALDIESGIPLIDEPNCDSFLPQAVNLQALGGISFTKGCYSGQEMVARA
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHCCCCCCHHHHHHH
KYRGANRRALFWLRGSAERLPHASEDLELRLGDSWRRSGTVLAAQRLADGGVYLQAVLSS
HHCCCCCEEEEEECCCHHHCCCCCCCCEEEECCCCCCCCCEEEEHHHCCCCCEEEEEHHC
DLPADSVLRVRDDARSQLTLSPLPYSTDQQE
CCCHHHEEEECCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MNLNAPFSPQPPLAAEHLPLTLMRLDDWLPINVSGPDAQSYLQGQLTADLPSLAATQHTL
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHCCHHCCCCHHHHHHHHH
CGHCDAQGKLWSSLRLLRRRDGFTYLLRRSVATLQMLELKKYAVFAKASIVSDEGAVLLG
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEE
VAGAQASEALGALFPRLPDADAPLLQAGRSHLLYMAWPQPRYLLICDDADEAERIFAPLS
ECCCCHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECCCCCEEEEECCCCCHHHHHHCHH
ARARLADSAQWLALDIESGIPLIDEPNCDSFLPQAVNLQALGGISFTKGCYSGQEMVARA
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHCCCCCCHHHHHHH
KYRGANRRALFWLRGSAERLPHASEDLELRLGDSWRRSGTVLAAQRLADGGVYLQAVLSS
HHCCCCCEEEEEECCCHHHCCCCCCCCEEEECCCCCCCCCEEEEHHHCCCCCEEEEEHHC
DLPADSVLRVRDDARSQLTLSPLPYSTDQQE
CCCHHHEEEECCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA